Genotoxicity evaluation of environmental pollutants using analysis of nucleolar alterations

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Biochemical responses, morphometric changes, genotoxic effects and CYP1A expression in the armored catfish Pterygoplichthys anisitsi after 15 days of exposure to mineral diesel and biodiesel

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

In vitro mucus transportability, cytogenotoxicity, and hematological changes as non-destructive physiological biomarkers in fish chronically exposed to metals

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Hematological changes and cytogenotoxicity in the tilapia Oreochromis niloticus caused by sub-chronic exposures to mercury and selenium

Fish bioassays are valuable tools that can be used to elucidate the toxicological potential of numerous substances that are present in the aquatic environment. In this study, we assessed the antagonistic action of selenium (Se) against the toxicity of mercury (Hg) in fish (Oreochromis niloticus). Six experimental groups with six fish each were defined as follows: (1) control, (2) mercury (HgCl2), (3) sodium selenite (Na2Se4O3), (4) sodium selenate (Na2Se6O4), (5) mercury + sodium selenite (HgCl2 + Na2Se4O3), and (6) mercury + sodium selenate (HgCl2 + Na2Se6O4). Hematological parameters [red blood cells (RBC), white blood cells (WBC), and erythroblasts (ERB)] in combination with cytogenotoxicity biomarkers [nuclear abnormalities (NAs) and micronuclei (MN)] were examined after three, seven, ten, and fourteen days. After 7 days of exposure, cytogenotoxic effects and increased erythroblasts caused by mercury, leukocytosis triggered by mercury + sodium selenite, leukopenia associated with sodium selenate, and anemia triggered by mercury + sodium selenate were observed. Positive correlations that were independent of time were observed between WBC and RBC, ERB and MN, and NA and MN. The results suggest that short-term exposure to chemical contaminants elicited changes in blood parameters and produced cytogenotoxic effects. Moreover, NAs are the primary manifestations of MN formation and should be included in a class characterized as NA only. Lastly, the staining techniques used can be applied to both hematological characterization and the measurement of cytogenotoxicity biomarkers.

Allium cepa and Tradescantia pallida bioassays to evaluate effects of the insecticide imidacloprid

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Genotoxic responses of juvenile tilapia (Oreochromis niloticus) exposed to florfenicol and oxytetracycline

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Conventional and whitening toothpastes: Cytotoxicity, genotoxicity and effect on the enamel surface

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Mutagenic and genotoxic effect of hydroxyurea

The hydroxyurea, a cytotoxic drug, is the mainly available therapeutical strategy for the treatment of sickle cell disease. This study aimed to evaluate the mutagenic and genotoxic potential of the hydroxyurea through the Salmonella/Microsome assay and micronucleus test in peripheral blood of mice. The doses were evaluated at 29.25-468 μmol/plate in Salmonella/Microsome assay in presence and absence of metabolic activation the drug. In the micronucleus test the doses were evaluated at 12.5; 25; 50; 75 and 100 mg/kg. The results show that hydroxyurea present mutagenic activity in TA98 and TA100 in doses above 117 μmol/plate and 234 μmol/plate respectively. The drug induced a significant increase in the frequency of micronuclei in reticulocytes of mice at concentrations of 50, 75 and 100 mg/kg, compared to negative control (water). These results demonstrated the mutagenic and genotoxic potential of hydroxyurea.

Analysis of the genotoxic potential of low concentrations of Malathion on the Allium cepa cells and rat hepatoma tissue culture

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Avaliação da toxicidade da vinhaça tratada quimicamente utilizando Oreochromis niloticus (Perciformes: Cichlidae) como organismo teste

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Investigação dos efeitos tóxicos, citotóxicos, genotóxicos e mutagênicos do inseticida Curbix® 200SC (ethiprole) em organismos não alvos

Pós-graduação em Ciências Biológicas (Biologia Celular e Molecular) - IBRC

Descrição micromorfológica e efeitos do benzo[α]pireno em células hepáticas e sanguíneas das espécies Physalaemus cuvieri e Leptodactylus fuscus (Anura: Leptodactylidae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Avaliação de atividades biológicas dos extratos de Rosmarinus officinalis L. (alecrim) e Thymus vulgaris L. (tomilho)

Pós-graduação em Biopatologia Bucal - ICT

Análise da integridade genômica por meio do ensaio cometa e teste do micronúcleo em células-tronco mesenquimais derivadas do tecido adiposo

Pós-graduação em Biociências - FCLAS

Bioestimulação de célula-tronco mesenquimal de rato sob ação da luz contínua e pulsátil de 630 nm utilizando LED

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)