983 resultados para dt-RFLP


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Trägerband: 'Spirae 3a Julii 1655'; Vorbesitzer: Stadtarchiv Frankfurt am Main

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The first derivative of pressure over time (dP/dt) is a marker of left ventricular (LV) systolic function that can be assessed during cardiac catheterization and echocardiography. Radial artery dP/dt (Radial-dP/dt) has been proposed as a possible marker of LV systolic function (Nichols & O’Rourke, McDonald’s Blood Flow in Arteries) and we sought to test this hypothesis. Methods:We compared simultaneously recorded RadialdP/ dt (by high-fidelity tonometry) with LV-dP/dt (by highfidelity catheter and echocardiography parameters analogous to LV-dP/dt) in patients without aortic valve disease. In study 1, beat to beat Radial-dP/dt and LV-dP/dt were recorded at rest and during supine exercise in 12 males (aged 61±12 years) undergoing cardiac catheterization. In study 2, 2D-echocardiography and Radial-dP/dt were recorded in 59 patients (43 men; aged 64±10 years) at baseline and peak dobutamine-induced stress. Three measures at the basal septum were taken as being analogous to LV-dP/dt: (1) peak systolic strain rate, (2) strain rate (SR-dP/dt), and (3) tissue velocity during isovolumic contraction. Results: Study 1; there was a significant difference between resting LV-dP/dt (1461±383 mmHg/s) and Radial-dP/dt (1182±319 mmHg/s; P < 0.001), and a poor, but statistically significant, correlation between the variables (R2 = 0.006; P < 0.001) due to the high number of data points compared (n = 681). Similar results were observed during exercise. Study 2; there was a moderate association between baseline Radial-dP/dt and SRdP/ dt (R2 =−0.17; P < 0.01), but no significant relationship between Radial-dP/dt and all other echocardiographic measures analogous to LV-dP/dt at rest or peak stress (P > 0.05). Conclusion: The radial pressurewaveform is not a reliable marker of LV contractility.

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A partir da análise exegética de Deuteronômio 6,4-9, esta pesquisa aborda o significado da unicidade de Deus, sua relação com a Lei, em defesa do Único Deus representado por elas. Em princípio, pensamos em Deuteronômio 6,4-9 por ser um texto localizado em um dos livros mais importantes na formação do povo de Israel. A importância se faz em meio à constituição legal que rege a vida do povo, como também no sentimento que o povo nutre durante sua história posterior. Nossa investigação nos conduzirá ao Deus Único que hoje ainda sustentamos, com a percepção de sua presença, e em sua relevância. Nossa conclusão buscará compreender que o Deus Javé de Deuteronômio 6,4-9 ainda se faz presente e atuante, com sua participação efetiva que se move e caminha em meio ao seu povo, da mesma forma como chamou a atenção de Israel por meio da voz que convida a ouvir.

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A presente pesquisa tem como objeto de investigação o vocábulo lev na perícope de Dt 6,1-9. Este vocábulo também é utilizado em várias partes do Antigo Testamento. Assim a pesquisa também faz um levantamento deste uso na perspectiva de diversos autores. No primeiro capítulo se apresentam os blocos que constituem a moldura do livro do Deuteronômio com o objetivo de identificarmos o contexto maior onde encontra-se a perícope de Dt 6,1-9. No segundo capítulo partimos de uma análise exegética da perícope ressaltando os vários aspectos da linguagem, como estilo literário, gênero literário, e análise de conteúdo. Nesta parte também consta uma análise das realidades concretas subjacentes à produção do texto, como assento histórico, contexto social, político e ideológico. No terceiro capítulo apresentase o amplo campo semântico do vocábulo lev no Antigo Testamento na perspectiva de diversos autores. Tal delineação tem por objetivo explorar a polissemia do vocábulo, e assim neutralizá-la, buscando o sentido mais apropriado do vocábulo para a perícope de Dt 6,1-9.

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Féachann an tráchtas seo le solas a chaitheamh ar an amhránaíocht mar a chleachtaítí agus mar a chleactaítear fós i gcontae Phort Láirge í. Ardaítear ann ceisteanna a bhaineann le seachadadh agus le sealbhú na n-amhrán I measc an phobail i gceantar na nDéise sa tréimhse c.1750-1960, ó aimsir Sheáin Chláraigh go dtí an ré sin ina raibh Nioclás Tóibín, ‘rí-amhránaí Éireann’, ar bhuaic a réime. Cuirtear spéis anseo i bhfás agus i dteacht chun cinn an Rómánsachais agus (a leathchúpla) an náisiúnachais ar Mhór-roinn na hEorpa in earr an 18ú haois agus amach san 19ú haois; ar thionchar na ngluaiseachtaí sin i bhfad ó bhaile ar Éirinn i gcoitinne san aimsir úd; orthu sin a raibh díolamaí amhrán á gcur in eagar acu in Éirinn san 19ú agus amach san 20ú haois; agus, ar deireadh, ar an stór amhrán mar atá le clos inniu I measc na ndaoine i nGaeltacht na Déise.

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Neogobius caspius is a small benthic fish that is native to the Caspian Sea. The importance of this fish is because of it is role as a main food resource of the sturgeon fish. The genetic diversity of N. caspius population in the Caspian Sea was studied using PCR- RFLP technique. A total of 135 samples of N. caspius were collected from coastal line in the north Caspian sea, including specimens from coasts of Anzali , Torkman Port and Chalus. Genomic DNA was extracted by phenol-chloroform method and then was amplified using a pair primer of cytochrom b gene, 2 tRNA gene and the control region sequences by a thermal cycler. D2 (5'-CCGGAGTATGTAGGGCATTCTCAC-3'), CY1 (5'-YYTAACCRRGACYAATGACTTGA-3') 12 restriction enzyme were used to digest the target gene region including: Alul HincII —Tas1 —Rsa1 -MboI -DraI -BSeNI(BSRI) Alw261(BsmAI). Bsul 51 Hin11 Bsh12851- BsuRI(HaeIII) digested PCR products were observed by silver staining method followed by Polyacrylamide gel electrophoresis (PAGE). The results were shown the same pattern among the species. There was no polymorphism and no differentiation in population in the Neogobius caspius fish and all individuals have shown homogenous genotype.

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Resumen La deficiencia en la adhesión leucocitaria bovina (BLAD) y en la enzima arginosuccinato sintetasa (Citrulinemia) son enfermedades de herencia autosómica recesiva que han sido descritas a nivel mundial en la raza Holando. El objetivo de este estudio fue optimizar e implementar una metodología de genotipado para la identificación de animales portadores de los alelos causantes de estas enfermedades en una población cohorte de terneras de recría de la raza Holando de la cuenca lechera de Cerro Largo. Las muestras de ADN de 190 terneras Holando fueron extraídas a partir de sangre fresca, siguiendo normas y protocolos del Banco de ADN de la Unidad de Biotecnología INIA Las Brujas. El genotipado fue realizado mediante análisis PCR-RFLP con las enzimas de restricción TaqI para BLAD y Eco47I (AvaII) para Citrulinemia. La confirmación del genotipado fue evaluada mediante secuenciación de los productos amplificados de ambas enfermedades. Se detectó la presencia del alelo mutante para BLAD en una sola ternera de recría y no se encontró portadoras de Citrulinemia en la población analizada. Este trabajo representó el primer relevamiento de la prevalencia génica de las enfermedades BLAD y Citrulinemia en la región Este del Uruguay

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Dt 4, 1-40 it a Biblical text particularly relevant, both for its location and sense within the Deuteronomy book, as well as for its relation with the overall Deuteronomist literature. However, we do not handle in-depth and extensive studies of this text, with exception of the works published by G. Braulik516, D. Knapp517 and K. Holter518, and other exploratory studies much thoroughly investigated, as well as small monographic ones specified in a particular matter. On the other hand, the investigation of the text has been focused mainly around the historical and theological analysis of it, with the purpose to determine the time in which the text was introduced in the total of the book, as well as to weight the significance of the different stratum of the text, its sources... For this reason, other medium of approach to the text has been left aside, or had been used only as instruments to be served to the main purpose of this study. This has been for instance the study of the literary analysis. Nevertheless, during these last years, the literary investigation of the biblical texts (linguistics, narrative, rhetoric, comparative literature...) has gained boom, and had allowed a great appreciation of these texts, overall its historical or theological relevance. Dt 4, 1-40 has been benefit from all of it. The studies dedicated to the literary analysis of Dt 4, 1-40 show considerable patterns on the ways they had been carried. We have analysed them from the syntax and narrative points of view, fields very little investigated up to now. We believe it is necessary to study the text from these two perspectives to appreciate the wealth of this literary composition beyond the topics covered on it, and to contribute this way to a deep investigation of such a significant text in shape and content for the present Biblical Philology. The final objective of our study it is to arrive to a full comprehension of the thematic and literary unity of the text through its syntactic and narrative analysis, and, at the same time, to determine the mutual and necessary relationship that exist between one to another in this line of investigation...

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Méthodologie: Recherche qualitative de type phénoménologique, ethnographique (Fortin, Côté, & Filion, 2006) ; Interactionnisme symbolique (Poisson, 1992)

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Fusarium decemcellulare é encontrado como agente causal de doenças com diferentes sintomas em diversas espécies de plantas em regiões tropicais e subtropicais. Em guaranazeiro, espécie nativa da Amazônia de importância econômica e social, a doença denominada de complexo superbrotamento é atualmente um dos principais problemas da cultura. Técnicas moleculares são cada vez mais requeridas para identificação rápida e segura de patógenos como complemento as técnicas convencionais. O objetivo do trabalho foi desenvolver métodos moleculares por PCR e PCR-RFLP para rápida identificação de F. decemcellulare.

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The genetic diversity of E. oleifera is strongly structured by geographical origin, with four groups clearly distinguished: Brazil, Surinam/French Guyana, north of /Colombia/Central America and Peru. Within the Amazon basin, thereis a moderate structure that corresponds to the major tributaries of the Amazon river. From the 37 polymorphic RFLP probe/enzyme combinatios used, 19 probes (51%) presented simple restriction profiles, with one (1) or two bands/plant, suggesting a single locus with different alleles, allowing allelic co-dominant coding for them.

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L’avvento dell’Internet of Things, in parallelo con il sempre più diffuso fenomeno della sensorizzazione, ha aperto la strada allo sviluppo di numerose tecnologie, tra le quali sono presenti anche i Digital Twin. Se inizialmente l’idea di Digital Twin era limitata a mantenere una copia virtuale di un oggetto fisico, con il passare del tempo questa visione si è ampliata, permettendo di riprodurre in un ambiente virtuale interi sistemi, come ad esempio città ed ospedali. Questi sistemi sono costituiti da vere e proprie reti di Digital Twin, tra loro connessi attraverso l’uso di relazioni. A questo proposito le sfide sono presenti e numerose: quel che manca è una visione unica e condivisa riguardante l’organizzazione di tali sistemi. Sotto questo aspetto, una delle proposte è il Web of Digital Twin, di cui verrà discusso all’intero della tesi. L’obiettivo di questa tesi è quello di capire come è possibile realizzare ecosistemi di Digital Twin coerenti con i principi del WoDT, attraverso la piattaforma Azure Digital Twin. A tale scopo è anche esposto un caso di studio pratico che comprende la prototipazione di un sistema di questo tipo.

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Desmoid tumor (DT) is a common manifestation of Gardner's Syndrome (GS), although it is a rare condition in the general population. DT in patients with GS is usually located in the abdominal wall and/or intra-abdominal cavity. We report a case of a 32 years-old female patient with familial adenomatous polyposis (FAP), who was already submitted to total colectomy and developed multiple DT, located in the abdominal wall and in the left breast. The patient underwent several surgical procedures, with a multidisciplinary team of surgeons. Wide surgical resections of the left breast and the abdominal wall tumors were performed in separate steps. Polypropylene mesh reconstruction and muscle flaps were needed to cover the defects of the thoracic and abdominal walls. After partial necrosis of the adipose-cutaneous flap in the abdomen that required a new skin graft, she had a satisfactory outcome with complete healing of the surgical incisions. DT is frequent in GS, however, breast localization is very rare, with few cases reported in the literature. Recurrence of DT is not negligible, even after a wide surgical resection. GS patients must be followed up closely, and clinical examination, associated with imaging studies, should be performed to detect any signs of tumor. DT represents one of the most significant causes of the morbidity and mortality that affects FAP patients following colectomy. In general, the surgical procedures to excise DT are highly complex, requiring a multidisciplinary team.

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To evaluate associations between polymorphisms of the N-acetyltransferase 2 (NAT2), human 8-oxoguanine glycosylase 1 (hOGG1) and X-ray repair cross-complementing protein 1 (XRCC1) genes and risk of upper aerodigestive tract (UADT) cancer. A case-control study involving 117 cases and 224 controls was undertaken. The NAT2 gene polymorphisms were genotyped by automated sequencing and XRCC1 Arg399Gln and hOGG1 Ser326Cys polymorphisms were determined by Polymerase Chain Reaction followed by Restriction Fragment Length Polymorphism (PCR-RFLP) methods. Slow metabolization phenotype was significantly associated as a risk factor for the development of UADT cancer (p=0.038). Furthermore, haplotype of slow metabolization was also associated with UADT cancer (p=0.014). The hOGG1 Ser326Cys polymorphism (CG or GG vs. CC genotypes) was shown as a protective factor against UADT cancer in moderate smokers (p=0.031). The XRCC1 Arg399Gln polymorphism (GA or AA vs. GG genotypes), in turn, was a protective factor against UADT cancer only among never-drinkers (p=0.048). Interactions involving NAT2, XRCC1 Arg399Gln and hOGG1 Ser326Cys polymorphisms may modulate the risk of UADT cancer in this population.