957 resultados para agricultural and horticultural pest
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Another ed. of the same date has 357 p.
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[v.2] Supporting appendixes.
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Mode of access: Internet.
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Mode of access: Internet.
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Bibliography: p. 357-378.
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First edition.
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"New York, D. Van Nostrand company" stamped on t.-p.
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Mode of access: Internet.
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Mode of access: Internet.
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Mode of access: Internet.
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Volumes for 1922- also called 4th ed.-
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Prentice-Hall journalism series.
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Includes "References."
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Phytophthora diseases cause major losses to agricultural and horticultural production in Australia and worldwide. Most Phytophthora diseases are soilborne and difficult to control, making disease prevention an important component of many disease management strategies. Detection and identification of the causal agent, therefore, is an essential part of effective disease management. This paper describes the development and validation of a DNA-based diagnostic assay that can detect and identify 27 different Phytophthora species. We have designed PCR primers that are specific to the genus Phytophthora. The resulting amplicon after PCR is subjected to digestion by restriction enzymes to yield a specific restriction pattern or fingerprint unique to each species. The restriction patterns are compared with a key comprising restriction patterns of type specimens or representative isolates of 27 different Phytophthora species. A number of fundamental issues, such as genetic diversity within and among species which underpin the development and validation of DNA-based diagnostic assays, are addressed in this paper.