Trypanosoma congolense procyclins: unmasking cryptic major surface glycoproteins in procyclic forms

In the tsetse fly, the protozoan parasite Trypanosoma congolense is covered by a dense layer of glycosylphosphatidylinositol (GPI)-anchored molecules. These include a protease-resistant surface molecule (PRS), which is expressed by procyclic forms early in infection, and a glutamic acid- and alanine-rich protein (GARP), which appears at later stages. Since neither of these surface antigens is expressed at intermediate stages, we investigated whether a GPI-anchored protein of 50 to 58 kDa, previously detected in procyclic culture forms, might constitute the coat of these parasites. We therefore partially purified the protein from T. congolense Kilifi procyclic forms, obtained an N-terminal amino acid sequence, and identified its gene. Detailed analyses showed that the mature protein consists almost exclusively of 13 heptapeptide repeats (EPGENGT). The protein is densely N glycosylated, with up to 13 high-mannose oligosaccharides ranging from Man(5)GlcNAc(2) to Man(9)GlcNAc(2) linked to the peptide repeats. The lipid moiety of the glycosylphosphatidylinositol is composed of sn-1-stearoyl-2-lyso-glycerol-3-HPO(4)-1-(2-O-acyl)-d-myo-inositol. Heavily glycosylated proteins with similar repeats were subsequently identified in T. congolense Savannah procyclic forms. Collectively, this group of proteins was named T. congolense procyclins to reflect their relationship to the EP and GPEET procyclins of T. brucei. Using an antiserum raised against the EPGENGT repeat, we show that T. congolense procyclins are expressed continuously in the fly midgut and thus form the surface coat of cells that are negative for both PRS and GARP.

Die Mehrstimmige Messe im ‘Goldenen Jahrhundert’. Überlieferung und Repertoirebildung in Quellen aus Spanien und Portugal (ca. 1490-1630)

Das Studium der Überlieferung und Repertoirbildung gibt über die Art der Nutzung von musikalischen Werken Aufschluss. Im iberischen Raum wurde im 16. Jh. das internationale Messenrepertoire aus Italien und Flamen ausgiebig genutzt, doch waren die kommerziellen Wege für die Beziehung wichtiger als politische Allianzen.

Ein merkwürdiger Vorfall in der Judengemeinde zu Worms im J. 1430

Christian Wilhelm Spieker

Allogeneic haematopoietic stem cell transplantation for mitochondrial neurogastrointestinal encephalomyopathy.

Haematopoietic stem cell transplantation has been proposed as treatment for mitochondrial neurogastrointestinal encephalomyopathy, a rare fatal autosomal recessive disease due to TYMP mutations that result in thymidine phosphorylase deficiency. We conducted a retrospective analysis of all known patients suffering from mitochondrial neurogastrointestinal encephalomyopathy who underwent allogeneic haematopoietic stem cell transplantation between 2005 and 2011. Twenty-four patients, 11 males and 13 females, median age 25 years (range 10-41 years) treated with haematopoietic stem cell transplantation from related (n = 9) or unrelated donors (n = 15) in 15 institutions worldwide were analysed for outcome and its associated factors. Overall, 9 of 24 patients (37.5%) were alive at last follow-up with a median follow-up of these surviving patients of 1430 days. Deaths were attributed to transplant in nine (including two after a second transplant due to graft failure), and to mitochondrial neurogastrointestinal encephalomyopathy in six patients. Thymidine phosphorylase activity rose from undetectable to normal levels (median 697 nmol/h/mg protein, range 262-1285) in all survivors. Seven patients (29%) who were engrafted and living more than 2 years after transplantation, showed improvement of body mass index, gastrointestinal manifestations, and peripheral neuropathy. Univariate statistical analysis demonstrated that survival was associated with two defined pre-transplant characteristics: human leukocyte antigen match (10/10 versus <10/10) and disease characteristics (liver disease, history of gastrointestinal pseudo-obstruction or both). Allogeneic haematopoietic stem cell transplantation can restore thymidine phosphorylase enzyme function in patients with mitochondrial neurogastrointestinal encephalomyopathy and improve clinical manifestations of mitochondrial neurogastrointestinal encephalomyopathy in the long term. Allogeneic haematopoietic stem cell transplantation should be considered for selected patients with an optimal donor.

Ausst. 328 - Ablaßbrief zum Besten des Kampfes gegen die Türken, 1490

Vorbesitzer: Ulrich Neuhaus (Ulricus Nuhuß) de Franckfordia"

Isabel Dunlo, Brustbild, Profil

Signatur des Originals: S 36/F08489

Isabel Dunlo, Brustbild

Signatur des Originals: S 36/F08490

Na 50 Nachlass Arthur Schopenhauer - 'Schopenhauer-Archiv' 1430 - Universitätsvorlesung: Metaphysik des Schönen

Bereitgestellt mit freundlicher Unterstützung der Staatsbibliothek zu Berlin - Preußischer Kulturbesitz

La misión fundante de los apodos en La Reina Isabel cantaba rancheras de Rivera Letelier

En su novela La Reina Isabel cantaba rancheras, Rivera Letelier retrata el sórdido mundo de las pampas salitreras del norte de Chile. Los personajes que habitan estas regiones carecen por completo de nombre propio. En su lugar encontramos apodos a partir de los que el autor crea y recrea la historia de los viejos y prostitutas que habitaron estas oficinas salitreras. Más allá de la evidente intención cómica que persigue el autor de la novela, creemos que el apodo es el elemento estructurante tanto de los personajes como de la novela en general. Esto se debe a que la historia de la desaparición de la "última oficina salitrera" se entrelaza con la historia de los personajes que vivieron en ella. Los protagonistas de la novela se destacan gracias a sus apodos del resto de aquellos viejos que no hicieron ningún mérito para conseguirlo y por lo tanto están condenados al anonimato. Precisamente, son las características del apodo las que permiten al autor configurar a estos personajes que al lector le costará olvidar. Como primera característica, aludiremos a la calidad referencial del apodo frente al nombre propio. Esto se debe a que el mote surge de los defectos, virtudes o de cualquier otra circunstancia vivida por alguna persona. Esto permite que se establezca una relación causal entre apodo y persona. Por su parte, el nombre propio establece una relación casual con la persona a la que designa. Por otro lado, mientras el nombre propio es otorgado de una vez y para siempre. El apodo presenta como segunda característica la validez. Es decir, el apodo, necesariamente, esta circuscripto a un tiempo, a un espacio y sólo es válido para un grupo determinado. Esto se debe a que sólo dentro de estos parámetros el apodo podrá ser interpretado. Cuando se altera alguna de estas coordenadas, indefectiblemente, el apodo cambia.

Horizontal profiles of longwave and shortwave radiation components over sea ice near Barrow, Alaska during the 2011 melt

An integrated instrument package for measuring and understanding the surface radiation budget of sea ice is presented, along with results from its first deployment. The setup simultaneously measures broadband fluxes of upwelling and downwelling terrestrial and solar radiation (four components separately), spectral fluxes of incident and reflected solar radiation, and supporting data such as air temperature and humidity, surface temperature, and location (GPS), in addition to photographing the sky and observed surface during each measurement. The instruments are mounted on a small sled, allowing measurements of the radiation budget to be made at many locations in the study area to see the effect of small-scale surface processes on the large-scale radiation budget. Such observations have many applications, from calibration and validation of remote sensing products to improving our understanding of surface processes that affect atmosphere-snow-ice interactions and drive feedbacks, ultimately leading to the potential to improve climate modelling of ice-covered regions of the ocean. The photographs, spectral data, and other observations allow for improved analysis of the broadband data. An example of this is shown by using the observations made during a partly cloudy day, which show erratic variations due to passing clouds, and creating a careful estimate of what the radiation budget along the observed line would have been under uniform sky conditions, clear or overcast. Other data from the setup's first deployment, in June 2011 on fast ice near Point Barrow, Alaska, are also shown; these illustrate the rapid changes of the radiation budget during a cold period that led to refreezing and new snow well into the melt season.