Coplas por las folias, y otras por el A, B, C. [Texto impreso]

Contiene: Glosas de un amante, que se despide de su dama, hechas por el A, B, C ; Respuesta de la Dama en las otras letras restantes del abecedario

Triplex targeting of human PDGF-B (c-sis, proto-oncogene) promoter specifically inhibits factors binding and PDGF-B transcription

Human c-sis/PDGF-B proto-oncogene has been shown to be overexpressed in a large percentage of human tumor cells establishing a growth-promoting, autocrine growth circuit. Triplex forming oligonucleotides (TFOs) can recognize and bind sequences in duplex DNA, and have received considerable attention because of their potential for targeting specific genomic sites. The c-sis/PDGF-B promoter contains a unique homopurine/homopyrimidine sequence (SIS proximal element, SPE), which is crucial for binding nuclear factors that provoke transcription. In order to develop specific transcriptional inhibitors of the human c-sis/PDGF-B proto-oncogene, 20 potential TFOs targeting part or all of the SPE were screened by gel mobility analysis. DNase I footprinting shows that the TFOs we designed can form a sequence-specific triplex with the target. Protein binding assays demonstrate that triplex formation inhibits nuclear factors binding the c-sis/PDGF-B promoter. Both transient and stable transfection experiments demonstrate that the transcriptional activity of the promoter is considerably inhibited by the TFOs. We propose that TFOs represent a therapeutic potential to specifically diminish the expression of c-sis/PDGF-B proto-oncogene in various pathologic settings where constitutive expression of this gene has been observed.

Crash protection systems for handicapped school and transit bus occupants. Volume III: appendices A, B, C and D. Interim report.

National Highway Traffic Safety Administration, Washington, D.C.

A B C of gothic architecture /

Includes index.

Sumerian mythology, a study of spiritual and literary achievement in the third millennium, B.C.

Frontispiece accompanied by leaf with descriptive letterpress.

Zenon papyri; business papers of the third century B.C. dealing with Palestine and Egypt,

Vol. 2 cite as: P.Col. IV

Report of the National Conference on Orphan Drugs : the path to promise : May 18-19, 1988, George Washington University, Washington, D.C. / [sponsored jointly by] the National Institutes of Health and the Pharmaceutical Manufacturers Association.

Mode of access: Internet.

Dedications from the Athenian Akropolis; a catalogue of the inscriptions of the sixth and fifth centuries B. C.

Includes bibliographical references.

Geology and ore-deposits of Rossland, B.C. /

Vita.

A B C of Gothic architecture /

Includes index.