Avaliação de um método de cromatografia em fase gasosa head space e estudo da estabilidade do etanol em amostras de sangue

The ethanol is the most overused psychoactive drug over the world; this fact makes it one of the main substances required in toxicological exams nowadays. The development of an analytical method, adaptation or implementation of a method known, involves a process of validation that estimates its efficiency in the laboratory routine and credibility of the method. The stability is defined as the ability of the sample of material to keep the initial value of a quantitative measure for a defined period within specific limits when stored under defined conditions. This study aimed to evaluate the method of Gas chromatography and study the stability of ethanol in blood samples, considering the variables time and temperature of storage, and the presence of preservative and, with that check if the conditions of conservation and storage used in this study maintain the quality of the sample and preserve the originally amount of analyte present. Blood samples were collected from 10 volunteers to evaluate the method and to study the stability of ethanol. For the evaluation of the method, part of the samples was added to known concentrations of ethanol. In the study of stability, the other side of the pool of blood was placed in two containers: one containing the preservative sodium fluoride 1% and the anticoagulant heparin and the other only heparin, was added ethanol at a concentration of 0.6 g/L, fractionated in two bottles, one being stored at 4ºC (refrigerator) and another at -20ºC (freezer), the tests were performed on the same day (time zero) and after 1, 3, 7, 14, 30 and 60 days of storage. The assessment found the difference in results during storage in relation to time zero. It used the technique of headspace associated with gas chromatography with the FID and capillary column with stationary phase of polyethylene. The best analysis of chromatographic conditions were: temperature of 50ºC (column), 150ºC (jet) and 250ºC (detector), with retention time for ethanol from 9.107 ± 0.026 and the tercbutanol (internal standard) of 8.170 ± 0.081 minutes, the ethanol being separated properly from acetaldehyde, acetone, methanol and 2-propanol, which are potential interfering in the determination of ethanol. The technique showed linearity in the concentration range of 0.01 and 3.2 g/L (0.8051 x + y = 0.6196; r2 = 0.999). The calibration curve showed the following equation of the line: y = x 0.7542 + 0.6545, with a linear correlation coefficient equal to 0.996. The average recovery was 100.2%, the coefficients of variation of accuracy and inter intra test showed values of up to 7.3%, the limit of detection and quantification was 0.01 g/L and showed coefficient of variation within the allowed. The analytical method evaluated in this study proved to be fast, efficient and practical, given the objective of this work satisfactorily. The study of stability has less than 20% difference in the response obtained under the conditions of storage and stipulated period, compared with the response obtained at time zero and at the significance level of 5%, no statistical difference in the concentration of ethanol was observed between analysis. The results reinforce the reliability of the method of gas chromatography and blood samples in search of ethanol, either in the toxicological, forensic, social or clinic

Demanda energética e eficiência da distribuição de sementes de milho sob variação de velocidade e condição de solo

O trabalho teve o objetivo de avaliar a demanda energética e a eficiência da distribuição de sementes de uma semeadora-adubadora para semeadura direta, submetida à variação de velocidade e condições de solo, na semeadura da cultura do milho. O estudo foi desenvolvido em um Nitossolo Vermelho distrófico, na Fazenda Experimental Lageado, no município de Botucatu - SP. O delineamento experimental foi em blocos casualizados, com oito repetições, combinando-se três velocidades de deslocamento (4,4; 6,1 e 8,1 km h-1) e duas condições de solo (solo manejado sob sistema de plantio direto há cinco anos e solo preparado com escarificador há 18 meses). Avaliaram-se a força de tração, a potência na barra de tração, o consumo de combustível, a capacidade de campo efetiva, a distribuição longitudinal de plantas, o coeficiente de variação, o índice de precisão e o número de plantas por hectare (estande inicial). Os resultados revelaram que, aumentando-se a velocidade de 4,4 para 8,1 km h-1, consegue-se aumentar em 86% a capacidade operacional, com incremento de 96% na demanda de potência na barra de tração e redução de 26% no consumo operacional de combustível. A maior velocidade (8,1 km h-1) proporcionou menor porcentual de espaçamentos normais e aumento no porcentual de espaçamentos múltiplos e falhos, maior coeficiente de variação e pior índice de precisão. A variação da velocidade não interferiu no número de plantas por hectare.

Genetic diversity of two Brazilian populations of the Pampas deer (Ozotoceros bezoarticus, Linnaeus 1758)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeito do manejo sobre a produção e composição química de perfilhos do capim-colonião (Panicum maximum Jacq.)

O experimento foi conduzido com o objetivo de verificar os efeitos das freqüências de corte de 28, 35 e 42 dias, obtidas em dezembro, janeiro e fevereiro, cortadas rente a 15 cm e a 30 cm do solo, sobre a produção e composição química de perfilhos do capim-colonião. A área experimental foi adubada na semeadura e por ocasião dos cortes de uniformização. Verificou-se que a produção de MS foi o principal fator da produção de nutrientes por área. Os teores de PB dos perfilhos apresentaram comportamento não-linear com o avanço da idade e diminuíram sensivelmente a baixas alturas e altas freqüências de corte. Os teores de FDA dos perfilhos variaram pouco entre as idades de 28 e 42 dias. Concluiu-se que o melhor manejo do capim-colonião seria o corte a cada 42 dias e a 15 cm do solo.

Trends in the karyotypic evolution of the Neotropical catfish family Heptapteridae Bockmann 1998 (Teleostei: Siluriformes)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Fitossociologia e distribuição de espécies arbóreas em uma floresta ribeirinha secundária no município de Rio Claro, SP, Brasil

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Genetic variability in natural populations of Zeyheria montana mart. from the Brazilian Cerrado

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Phylogenetic relationship of Sirodotia species (Batrachospermales, Rhodophyta) in North and South America

Sequence data from the RUBISCO large subunit (rbcL) plastid gene and nuclear small-subunit ribosomal DNA (SSU rDNA) were examined for five samples of Sirodotia delicatula from southeastern Brazil. Data from six North American samples previously identified as S. huillensis and S. suecica were also included in the analysis. Molecular data supported the continued recognition of these three species as separate entities, although one of the North American collections was misidentified. These results were shown to be congruent with morphology, chromosome number and geographic distribution. S. delicatula is more closely related to S. huillensis, both occurring in tropical-subtropical regions, than either to S. suecica with a temperate-boreal distribution. There was little rbcL variation within S. delicatula from Brazil and Costa Rica (the latter a collection previously identified as S. huillensis), with the six samples sequenced diverging from each other by 0-8 bp (0-0.67%). SSU rDNA data set did not provide sufficient resolution to infer phylogenetic relationships among the species of this group due to the low rates of variation (5 bp). Sirodotia was a well-supported clade (100% bootstrap or 1.00 a posteriori probability) based on rbcL sequences. Thus, the results confirm that Sirodotia is a monophyletic group within the Batrachospermales and we continue to recognize it at the generic level. The species S. delicatula, S. huillensis and S. suecica are morphologically and genetically distinct.

COMPARISON OF 3 TECHNIQUES FOR ESTIMATING PERIPHYTON ABUNDANCE IN BEDROCK STREAMS

Three procedures (line transect, point intercept and quadrat) for estimating abundance of macroscopic components of periphyton (macroalgae and bryophytes) were compared in two bedrock streams from southeastern Brazil on the basis of frequency and percent cover. In addition, two quadrat sizes (25 and 50 cm) were tested for best size. Samplings were all made within a 10 m length stream reach. Differences were tested by means of analysis of variance, ANOVA - one way, Student's t test and Chi-square test, whereas associations among them were evaluated by Pearson's r correlation coefficient. Values of frequency and percent cover varied among the different techniques and periphyton species in both sites but ANOVA, t and Chi-square tests revealed no significant difference (p<0.05) for percent cover in the two sites. No consistent pattern was observed for the different procedures between sites. Values for quadrat (25 and 50 cm) percent cover were positively correlated among distinct periphyton species. Significant differences for frequency were found in only one site. Quadrats of 50 cm produced the highest frequency values, whereas point intercept the lowest in both sites. In terms of spatial variation within the stream segment, significant differences were generally observed among all procedures, except between quadrats. As a rule, highly significant correlations for percent cover were found among the different techniques along the stream reach. The strongest relationships were found for quadrats with the other procedures, whereas the weakest between Line and point. Pros and cons of the three techniques are fully discussed.

Modelagem da variação horária da temperatura do ar em Petrolina, PE, e Botucatu, SP

The air temperature is a climatic element of interest for several agricultural related studies. Hence, daily air temperature from two locations in Brazil (Botucatu and Petrolina, with different climate classification), hourly recorded from January 2004 to December 2005, were analysed to verify if 24 h long air temperature variation can be predicted with 6 h interval records. Three data set were selected, with different first time record of air temperature, and analyses performed using geoestatistics to confirm the temporal dependency of records. Then, ordinary krigging was used to model air temperature variation within 1 h interval, and spherical model presented the best approach, with range of 11.6 h for Botucatu and 12.7 for Petrolina. For both locations, the time of air temperature recording was the most importnat factor on accuracy of estimation of air temperature variation. Records at 2, 8, 14 and 20 h and at 4, 10, 16 and 22 h presented the best approaches, but for records at 9, 15 and 21 h, the approach was worst.

Biogeography of common dolphins (genus Delphinus) in the Southwestern Atlantic Ocean

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Hemoglobinas anormais e dificuldade diagnóstica

The human hemoglobins, with genetically defined inheritance patterns, have shown characteristic polymorphic variation within the Brazilian population, depending on the racial groups of each region. They have appeared under the form of hemoglobin variants or thalassemias, the variant types S and C and the alpha and beta thalassemias being more common, all of them in heterozygote form. During the year of 1999, blood samples from 506 individuals, with suspected anemia or that had already passed through hemoglobinopathies screening, were sent to the Hemoglobin Reference Center - UNESP for diagnostic confirmation and submitted to electrophoresis proceedings, biochemical and cytological analyses in order to characterize the type of abnormal hemoglobins. The goal of the present study was to verify which abnormal hemoglobin types show greater diagnostic difficulty. The samples came from 24 cities in twelve states. The results showed that 354 (69.96%) individuals presented abnormal hemoglobins, 30 (5.93%) being Hb AS, 5 (0.98%) being Hb AC, 76 (15.02%) suggestive of heterozygote alpha thalassemia, 134 (26.48%) suggestive of heterozygote beta thalassemia and 109 (21.54%) with other forms of abnormal hemoglobin, including rare variants and different forms of thalassemias and variant hemoglobin interactions. It has been concluded that, despite the improved techniques currently available and a constant influx of capacitated personnel, the heterozygote form of thalassemias (210 individuals -41.50%) is challenging to diagnose, followed in difficulty by rare variant characterization and interactive forms of hemoglobinopathies (109 individuals-21,54%), suggesting that the capacity for production of qualified professionals and information about these genetic changes in our population should be increased.

An assessment of the economic impact of climate change on the water sector in Grenada

Changing precipitation patterns and temperature relate directly to water resources and water security. This report presents the findings of an assessment of the water sector in Grenada with respect to the projected impact of climate change. Grenada‘s water resources comprise primarily surface water, with an estimated groundwater potential to satisfy about 10%-15% of the present potable requirement. On the smaller islands Carriacou and Petite Martinique, domestic water is derived exclusively from rainwater catchments. Rainfall seasonality is marked and the available surface water during the dry season declines dramatically. Changing land use patterns, increase in population, expansion in tourism and future implementation of proposed irrigation schemes are projected to increase future water requirements. Economic modeling approaches were implemented to estimate sectoral demand and supply between 2011 and 2050. Residential, tourism and domestic demand were analysed for the A2, B2 and BAU scenarios as illustrated. The results suggest that water supply will exceed forecasted water demand under B2 and BAU during all four decades. However under the A2 scenario, water demand will exceed water supply by the year 2025. It is important to note that the model has been constrained by the omission of several key parameters, and time series for climate indicators, data for which are unavailable. Some of these include time series for discharge data, rainfall-runoff data, groundwater recharge rates, and evapotranspiration. Further, the findings which seem to indicate adequacy of water are also masked by seasonality in a given year, variation from year to year, and spatial variation within the nation state. It is imperative that some emphasis be placed on data generation in order to better project for the management of Grenada‘s water security. This analysis indicates the need for additional water catchment, storage and distribution infrastructure, as well as institutional strengthening, in order to meet the future needs of the Grenadian population. Strategic priorities should be adopted to increase water production, increase efficiency, strengthen the institutional framework, and decrease wastage. Grenada has embarked on several initiatives that can be considered strategies toward adaptation to the variabilities associated with climate change. The Government should ensure that these programs be carried out to the optimal levels for reasons described above. The ―no-regrets approach‖ which intimates that measures will be beneficial with or without climate change should be adopted. A study on the Costs of Inaction for the Caribbean in the face of climate change listed Grenada among the countries which would experience significant impacts on GDP between now and 2100 without adaptation interventions. Investment in the water sector is germane to building Grenada‘s capacity to cope with the multivariate impact of changes in the parameters of climate.

Sistema plantio direto: população de plantas de milho e velocidade de semeadura em consórcio com braquiárias

Pós-graduação em Agronomia (Ciência do Solo) - FCAV

Desempenho operacional de máquinas agrícolas na implantação da cultura do sorgo forrageiro

Pós-graduação em Agronomia (Energia na Agricultura) - FCA