Evaluation of colon cancer histomorphology: a comparison between formalin and PAXgene tissue fixation by an international ring trial.

The aim of our study was to evaluate the quality of histo- and cytomorphological features of PAXgene-fixed specimens and their suitability for histomorphological classification in comparison to standard formalin fixation. Fifteen colon cancer tissues were collected, divided into two mirrored samples and either formalin fixed (FFPE) or PAXgene fixed (PFPE) before paraffin embedding. HE- and PAS-stained sections were scanned and evaluated in a blinded, randomised ring trial by 20 pathologists from Europe and the USA using virtual microscopy. The pathologists evaluated histological grading, histological subtype, presence of adenoma, presence of lymphovascular invasion, quality of histomorphology and quality of nuclear features. Statistical analysis revealed that the reproducibility with regard to grading between both fixation methods was rather satisfactory (weighted kappa statistic (k w) = 0.73 (95 % confidence interval (CI), 0.41-0.94)), with a higher agreement between the reference evaluation and the PFPE samples (k w = 0.86 (95 % CI, 0.67-1.00)). Independent from preservation method, inter-observer reproducibility was not completely satisfactory (k w = 0.60). Histomorphological quality parameters were scored equal or better for PFPE than for FFPE samples. For example, overall quality and nuclear features, especially the detection of mitosis, were judged significantly better for PFPE cases. By contrast, significant retraction artefacts were observed more frequently in PFPE samples. In conclusion, our findings suggest that the PAXgene Tissue System leads to excellent preservation of histomorphology and nuclear features of colon cancer tissue and allows routine morphological diagnosis.

Protesta di Tranquillo Corcos ... al tribunale della santa inquisizione contro il neofio D. Paolo Sebastiano Medici, predicatore

pubbl. da un ms. orig. del 1697 con corr. e note da Vittorio Castiglioni

Gedenkbuch der Frankfurter Juden nach Aufzeichnungen der Beerdigungs-Bruderschaft : 1624-80

hrsg. vom Komitee zur Erhaltung und Wiederherstellung der Grabdenkmäler auf dem alten israelitischen Friedhofe am Börneplatz zu Frankfurt am Main. Bearb. und ins Deutsche übertr. von Simon Unna

Ketubah (Ehevertrag) [Illustration] : Italien, 1694 Zitadella

Scan von Monochrom-Mikroform

Herbert Hesse als Paolo Orsini, Paul Helm als Cola Rienzi, Jean Stern als Steffano Colonna, Magda Spiegel als Adriano {[u.a.], Szenenfoto, Gruppenbildnis, in: Richard Wagner: Rienzi, der Letzte der Tribunen

Signatur des Originals: S 36/F11337

Paolo Becchi. Muerte cerebral y trasplantes de órganos. Un problema de ética jurídica, trad. E. Frontini & A. Núñez Vaquero, Madrid, Trotta, 2011, 156 pp.

Fil: Massini Correas, Carlos I.. Universidad de Mendoza

Horizontal profiles of longwave and shortwave radiation components over sea ice near Barrow, Alaska during the 2011 melt

An integrated instrument package for measuring and understanding the surface radiation budget of sea ice is presented, along with results from its first deployment. The setup simultaneously measures broadband fluxes of upwelling and downwelling terrestrial and solar radiation (four components separately), spectral fluxes of incident and reflected solar radiation, and supporting data such as air temperature and humidity, surface temperature, and location (GPS), in addition to photographing the sky and observed surface during each measurement. The instruments are mounted on a small sled, allowing measurements of the radiation budget to be made at many locations in the study area to see the effect of small-scale surface processes on the large-scale radiation budget. Such observations have many applications, from calibration and validation of remote sensing products to improving our understanding of surface processes that affect atmosphere-snow-ice interactions and drive feedbacks, ultimately leading to the potential to improve climate modelling of ice-covered regions of the ocean. The photographs, spectral data, and other observations allow for improved analysis of the broadband data. An example of this is shown by using the observations made during a partly cloudy day, which show erratic variations due to passing clouds, and creating a careful estimate of what the radiation budget along the observed line would have been under uniform sky conditions, clear or overcast. Other data from the setup's first deployment, in June 2011 on fast ice near Point Barrow, Alaska, are also shown; these illustrate the rapid changes of the radiation budget during a cold period that led to refreezing and new snow well into the melt season.