Occurrence of mycobacteria in bovine milk samples from both individual and collective bulk tanks at farms and informal markets in the southeast region of Sao Paulo, Brazil

Background: Mycobacterium spp. is one of the most important species of zoonotic pathogens that can be transmitted from cattle to humans. The presence of these opportunistic, pathogenic bacteria in bovine milk has emerged as a public-health concern, especially among individuals who consume raw milk and related dairy products. To address this concern, the Brazilian control and eradication program focusing on bovine tuberculosis, was established in 2001. However, bovine tuberculosis continues to afflict approximately 1,3 percent of the cattle in Brazil. In the present study, 300 samples of milk from bovine herds, obtained from both individual and collective bulk tanks and informal points of sale, were cultured on Löwenstein-Jensen and Stonebrink media. Polymerase chain reaction (PCR)-based tests and restriction-enzyme pattern analysis were then performed on the colonies exhibiting phenotypes suggestive of Mycobacterium spp., which were characterized as acid-fast bacilli.Results: Of the 300 bovine milk samples that were processed, 24 were positively identified as Mycobacterium spp.Molecular identification detected 15 unique mycobacterial species: Mycobacterium bovis, M. gordonae, M. fortuitum, M. intracellulare, M. flavescens, M. duvalii, M. haemophilum, M. immunogenum, M. lentiflavum, M. mucogenicum, M. novocastrense, M. parafortuitum, M. smegmatis, M. terrae and M. vaccae. The isolation of bacteria from the various locations occurred in the following proportions: 9 percent of the individual bulk-tank samples, 7 percent of the collective bulk-tank samples and 8 percent of the informal-trade samples. No statistically significant difference was observed between the presence of Mycobacterium spp. in the three types of samples collected, the milk production profiles, the presence of veterinary assistance and the reported concerns about bovine tuberculosis prevention in the herds.Conclusion: The microbiological cultures associated with PCR-based identification tests are possible tools for the investigation of the presence of Mycobacterium spp. in milk samples. Using these methods, we found that the Brazilian population may be regularly exposed to mycobacteria by consuming raw bovine milk and related dairy products. These evidences reinforces the need to optimize quality programs of dairy products, to intensify the sanitary inspection of these products and the necessity of further studies on the presence of Mycobacterium spp. in milk and milk-based products. © 2013 Franco et al.; licensee BioMed Central Ltd.

Incidence of Leptospirosis infection in the East Zone of Sao Paulo City, Brazil

Background: Leptospirosis is a zoonosis which is spread through contamined running water. This contaminations is seriously affected by the flooding which occurs in the area surrounding the Aricanduva river. The transmission of the disease results mainly from the contact of water with soil contaminated by the urine of infected animals. We aimed to conduct an epidemiological survey on Leptospirosis cases in Sao Paulo East Zone area. Method. The analysis conducted in this study was based on data collected from the health authorities of that region close the Aricanduva river between 2007 and 2008 years, which give the rates of confirmed cases, mortality and death from human Leptospirosis. Other information concerned with the relationships among rainfall index, points of flooding and incidence of Leptospirosis. Results: We observed a direct and important water contamination. Records of flooding points and dates of the reported cases in the region showed a direct relationship from which the period of higher rainfall also recorded an increase in cases. The annual record of the city and the region and rainfall regions also presented correlation. Conclusion: The association between the indices of flooding and Leptospirosis cases indicates that preventive measures are necessary to avoid exposing the community. © 2013 Miyazato et al.; licensee BioMed Central Ltd.

El plan de acción de Sao Paulo: una exposición sobre la técnica de planificación adoptada = The plan of action of Sao Paulo: a statement on the technique adopted for preparation of the plan

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Descripción de las actividades de información de la Fundación SEADE de Sao Paulo, Brasil

Contiene una descripción de objetivos y actividades en el área de población de la Fundación, información generada, unidades de información bibliográfica, procesamiento de datos y banco de datos, sugerencias de cooperación con otras instituciones.

Aspectos de la adaptación de los migrantes rurales a las condiciones urbano-industriales de Sao Paulo, Brasil = Aspects of the adjustment of rural migrants to urban-industrial conditions in Sao Paulo, Brazil

Los documentos del Seminario fueron publicados por UNESCO en 1961 con el título: La urbanización en América Latina/Urbanization in Latin America

Movimientos migratorios internos y problemas de acomodación del inmigrante nacional en Sao Paulo (Brasil)

Los documentos del Seminario fueron publicados por UNESCO en 1961 con el título: La urbanización en América Latina/Urbanization in Latin America

El plan de acción de Sao Paulo: una exposición sobre la técnica de planificación adoptada

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Conciliacion censal en una poblacion abierta: el caso del Estado de Sao Paulo, 1950-1970

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Elementos de la redistribucion espacial de la poblacion en el Estado de Sao Paulo-Brasil, 1960-1980; tesis de grado

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Estimación de la fecundidad en Brasil y en los Estados de Sao Paulo y Rio Grande do Norte en base a datos censales y de registro de los nacimientos vivos-1980; trabajo de investigación

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Estimaciones de mortalidad adulta femenina en Brasil, Sao Paulo y Bahía en base a datos de orfandad materna; trabajo de investigación

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Fecundidad y la nupcialidad en el Brasil y en los Estados de Sao Paulo y Rio Grande do Norte: tendencias pasadas y reflexiones sobre el comportamiento futuro de estas variables

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