Detecção dos genes integron, invA e spvC em Salmonella Enteritidis proveniente de material avícola e transferência horizontal do gene integron entre enterobactérias

Pós-graduação em Medicina Veterinária - FMVZ

Resposta imune celular e humoral em aves (Gallus gallus) vacinadas, antes e após o desafio com Salmonella enteritidis

Pós-graduação em Medicina Veterinária - FCAV

Experimental infection of one-day-old chicks with Salmonella Serotypes Previously isolated from poultry facilities, wild birds, and swine

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Transferência de Salmonella Enteritidis por contaminação cruzada e formação de biofilme em diferentes superfícies de corte

Pós-graduação em Biologia Geral e Aplicada - IBB

Streptococcus agalactiae in Brazil: serotype distribution, virulence determinants and antimicrobial susceptibility

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Detecção de Salmonella Enteritidis em ovos de codornas japonesas submetidas a dietas com diferentes níveis de cálcio e fósforo

The eggs are pointed in several studies as the main cause of human salmonellosis. Have been identified that eggs are eaten raw or poorly processed are mainly responsible for outbreaks of human infection with Salmonella spp. Besides causing problems to public health, the presence of bacteria impedes or hinders the international food trade, as a sanitary barrier. Several factors predisposing to contamination of the internal contents of eggs for Salmonella spp., including the egg shell quality (shell quality), which is related to levels of calcium (Ca) and phosphorus (P) in the diet of quails . The experiment used eggs of Japanese quail under different diets containing two levels of Ca (2.0 and 3.5%) and two levels of available P (0.25 and 0.45%). Eggs of 120 japanese quails were divided into four treatments with three replicates. The experiment was divided into three production stages: initial, intermediate and final. Were assessed at each stage the presence of bacteria in internal and external content of experimentally contaminated eggs during periods of 0, 24, 96 and 168 hours after immersion in broth containing Salmonella Enteriditis. Salmonella Enteriditis was detected in the shell during all periods of storage, in decreasing amounts in all treatments. None of the treatments within the three production stages analyzed, we detected significant amounts of the bacteria inside the egg, in our experimental conditions. Therefore, the levels of Ca and P in the diets of quail do not determine higher or lower risks to public health represented by eggs

Efeito da inoculação de probiótico in ovo sobre a morfometria intestinal e controle de salmonella enteritidis

Pós-graduação em Zootecnia - FMVZ

Infecção de aves (Gallus gallus domesticus) com Salmonella enterica subesp. enterica sorovar Enteritidis contendo deleções nos genes cobS, cbiA, pduC, pduD e pduE: avaliação da colonização intestinal, invasão sistêmica e resposta imune

Pós-graduação em Medicina Veterinária - FCAV

Efficacy of prebiotics, probiotics, and synbiotics on laying hens and broilers challenged with salmonella enteritidis

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Detection of CD4+ and CD8+ lymphocytes in the intestine of broiler chicks treated with lactobacillus spp. and challenged with Salmonella enterica serovar enteritidis

The expression of immune response as a leukocytic infiltrate by CD4+ and CD8+ cells in the epithelium and in the intestinal lamina propria of chicks fed Lactobacillus spp or cecal microflora (CM) and experimentally challenged or not with Salmonella enterica serovar Enteritidis (SE) was studied using immunohistochemistry. Three hundred and twenty day-of-hatch broiler chicks were divided into four groups of 80 birds each and orally received L. reuteri, L. salivarius, L. acidophilus, or CM. Each group was subdivided into four subgroups of 20 birds each, classified as follows: a subgroup did not receive any oral treatment (negative control), subgroup treated with L. spp or CM, subgroup treated with L. spp or CM and challenged with SE, and subgroup only challenged with SE (positive control). The results show that the oral treatment with L. reuteri, L. salivarius, L. acidophilus, or CM and challenge or not with SE stimulated bird immune response as determined by the leukocytic infiltrate by CD8+ lymphocytes followed by CD4+ in the epithelium and in the lamina propria of the duodenum, jejunum, and cecum of chicks up to 12 days of age. CD8+ lymphocyte number was significantly higher in the intestine of chicks receiving CM and challenged with SE. The duodenum, followed by the jejunum, were the segments in which the immune response, as shown by T, CD4+ and CD8+ cells, was stimulated with the greatest intensity.

Effects of 5-hydroxytryptophan and m-hydroxybenzylhydrazine associated to Lactobacillus spp. on the humoral response of broilers challenged with Salmonella Enteritidis

This study investigates the effects of different doses of serotonin, its precursor 5-hydroxytry-ptophan (5HTP), and m-hydroxybenzylhydrazine inhibitor (NSD1015), administered via intraperitoneal for 5 consecutive days, on behavior and average body weight of broilers. We also measured the humoral immune response and quantification of Salmonella Enteritidis in broilers chickens that received the drugs evaluated and a Lactobacillus pool. The study was divided into 3 experiments: Experiment 1--administration of pharmaceuticals with choice of dosage; Experiment 2--administration of pharmaceuticals and a Lactobacillus pool in birds that were not challenged with S. Enteritidis, and Experiment 3--administration of pharmaceuticals and a Lactobacillus pool in birds challenged with S. Enteritidis. The ELISA was used to scan dosages of intestinal IgA and serum IgY. We used colony-forming units to quantify S. Enteritidis. The concentrations of IgA and IgY did not show significant differences (P>0.05) in Experiment 2. In Experiment 3, NSD1015 associated with Lactobacillus determined higher IgA concentrations, promoting greater stimulus to the immune system than 5HTP. Regarding quantification of S. Enteritidis in the cecal content of birds, 5HTP associated to Lactobacillus determined the smallest number of bacteria, showing possible interaction of 5-hydroxytryptophan and Lactobacillus spp. with the immune system of broiler chickens.

Immunomodulatory activity and control of Salmonella Enteritidis colonization in the intestinal tract of chickens by Lactobacillus based probiotic

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Distribution of serotype-specific genotypes of Aggregatibacter actinomycetemcomitns in Brazilian patients with Down syndrome with different periodontal conditions

Fundação do Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Comparação de métodos para extração de dna na reação em cadeia da polimerase para detecção de Salmonella enterica sorovar enteritidis em produtos avícolas

The identification of Salmonella spp. in food samples by microbiological diagnosis is time consuming, with approximately five different stages, requiring about 120 hours until the final result. The utilization of the polymerase chain reaction technique (PCR) can reduce this time, but substances present in samples may affect the reaction. The present work aimed to compare DNA extraction by thermic treatment and by the use of cetyltrimethil ammonium bromide (CTAB), in products originated from poultry houses corresponding to raw material (meat meal) and experimentally contaminated drag swabs. Materials obtained from the extractions were submitted to PCR, utilizing a pair of initiator oligonucleotides for amplification of Sdf 1 gene fragments. Comparing the methods of extraction, it was observed that when CTAB was employed, SE was detected in 70% of meat meal and in 80% of drag swabs, while the thermic treatment method yielded positive results in 20% of meat meal and in 40% of drag swabs. SE was detected under both methods utilized for DNA extraction, but the use of CTAB detected a greater number of positive samples, compared with thermal treatment.