Gastroprotective effect of the ethanolic extract and fractions obtained from syngonanthus bisulcatus Rul

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

The Vampirome: Transcriptome and proteome analysis of the principal and accessory submaxillary glands of the vampire bat Desmodus rotundus, a vector of human rabies

Vampire bats are notorious for being the sole mammals that strictly feed on fresh blood for their survival. While their saliva has been historically associated with anticoagulants, only one antihemostatic (plasminogen activator) has been molecularly and functionally characterized. Here, RNAs from both principal and accessory submaxillary (submandibular) salivary glands of Desmodus rotundus were extracted, and ~. 200. million reads were sequenced by Illumina. The principal gland was enriched with plasminogen activators with fibrinolytic properties, members of lipocalin and secretoglobin families, which bind prohemostatic prostaglandins, and endonucleases, which cleave neutrophil-derived procoagulant NETs. Anticoagulant (tissue factor pathway inhibitor, TFPI), vasodilators (PACAP and C-natriuretic peptide), and metalloproteases (ADAMTS-1) were also abundantly expressed. Members of the TSG-6 (anti-inflammatory), antigen 5/CRISP, and CCL28-like (antimicrobial) protein families were also sequenced. Apyrases (which remove platelet agonist ADP), phosphatases (which degrade procoagulant polyphosphates), and sphingomyelinase were found at lower transcriptional levels. Accessory glands were enriched with antimicrobials (lysozyme, defensin, lactotransferrin) and protease inhibitors (TIL-domain, cystatin, Kazal). Mucins, heme-oxygenase, and IgG chains were present in both glands. Proteome analysis by nano LC-MS/MS confirmed that several transcripts are expressed in the glands. The database presented herein is accessible online at http://exon.niaid.nih.gov/transcriptome/D_rotundus/Supplemental-web.xlsx. These results reveal that bat saliva emerges as a novel source of modulators of vascular biology. Biological significance: Vampire bat saliva emerges as a novel source of antihemostatics which modulate several aspects of vascular biology. © 2013.

A novel function for CUGBP2 in controlling the pro-inflammatory stimulus in H9c2 cells: Subcellular trafficking of messenger molecules

Accumulating evidence demonstrates that chronic inflammation plays an important role in heart hypertrophy and cardiac diseases. However, the fine-tuning of cellular and molecular mechanisms that connect inflammatory process and cardiac diseases is still under investigation. Many reports have demonstrated that the overexpression of the cyclooxygenase-2 (COX-2), a key enzyme in the conversion of arachidonic acid to prostaglandins and other prostanoids, is correlated with inflammatory processes. Increased level of prostaglandin E2 was also found in animal model of left ventricle of hypertrophy. Based on previous observations that demonstrated a regulatory loop between COX-2 and the RNA-binding protein CUGBP2, we studied cellular and molecular mechanisms of a pro-inflammatory stimulus in a cardiac cell to verify if the above two molecules could be correlated with the inflammatory process in the heart. A cellular model of investigation was established and H9c2 was used.We also demonstrated a regulatory connection between COX-2 and CUGBP2 in the cardiac cells. Based on a set of different assays including gene silencing and fluorescence microscopy, we describe a novel function for the RNA-binding protein CUGBP2 in controlling the pro-inflammatory stimulus: subcellular trafficking of messenger molecules to specific cytoplasmic stress granules to maintain homeostasis. © 2013 International Federation for Cell Biology.

Produção de prostaglandinas e leucotrienos por Paracoccidioides brasiliensis

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efeito do geraniol sobre a doença ulcerosa péptica experimental

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Avaliação dos mecanismos de ação envolvidos nas atividades antiulcerogênica e cicatrizante do extrato etanólico obtido a partir das folhas de Terminalia catappa L. (COMBRETACEAE)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação do efeito antinociceptivo, anti-inflamatório e gastroprotetor do extrato hidroalcoolico das entrecascas de Machaerium hirtum (Vell) Stellfeld

Pós-graduação em Ciências Biológicas (Farmacologia) - IBB

Papel de prostaglandinas e leucotrienos sobre as funçoes das células dendrícas em resposta ao Paracoccidioides brasiliensis

Pós-graduação em Patologia - FMB

Estudo dos mecanismos envolvidos na migração celular induzida pelo hidróxido de cálcio para bolha de ar subcutânea em camundongos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Características e cronologia do parto induzido com cloprostenol ou d-cloprostenol em associação a carbetocina em cabras saanen

Pós-graduação em Medicina Veterinária - FMVZ

Cetoprofeno e expressão de citocinas TNF-alfa e IL-1 após hemorragia aguda em ratos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Papel da angiotensina II e das prostaglandinas no mecanismo de regulação da função renal de cães após ingestão de uma carga de proteína

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Identificação e purificação de prostaglandinas e leucotrienos produzidos por Paracoccidioides brasiliensis

Pós-graduação em Doenças Tropicais - FMB

Efeito de prostaglandinas sobre a atividade fingicida de monócitas humanos desafiados com o Paracoccidioides brasiliensis

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Ensaios pré-clínicos de híbridos ftalimídicos e pró-fármacos taurínicos derivados de antiinflamatórios não esteróides

Pós-graduação em Ciências Farmacêuticas - FCFAR