Hidrocarbonetos policíclicos aromáticos no meio ambiente: diferenciação de fontes em sedimentos e metabólitos em bile de peixes

Many studies on environmental ecosystems quality related to polycyclic aromatic hydrocarbons (PAH) have been carried out routinely due to their ubiquotus presence worldwide and to their potential toxicity after its biotransformation. PAH may be introduced into the environmet by natural and anthropogenic processes from direct runoff and discharges and indirect atmospheric deposition. Sources of naturally occurring PAHs include natural fires, natural oil seepage and recent biological or diagenetic processes. Anthropogenic sources of PAHs, acute or chronic, are combustion of organic matter (petroleum, coal, wood), waste and releases/spills of petroleum and derivatives (river runoff, sewage outfalls, maritime transport, pipelines). Besides the co-existence of multiples sources of PAH in the environmental samples, these compounds are subject to many processes that lead to geochemical fates (physical-chemical transformation, biodegradation and photo-oxidation), which leads to an alteration of their composition. All these facts make the identification of the hydrocarbons sources, if petrogenic, pyrolytic or natural, a challenge. One of the objectives of this study is to establish tools to identify the origin of hydrocarbons in environmental samples. PAH diagnostic ratios and PAH principal component analysis were tested on a critical area: Guanabara Bay sediments. Guanabara Bay is located in a complex urban area of Rio de Janeiro with a high anthropogenic influence, being an endpoint of chronic pollution from the Greater Rio and it was the scenario of an acute event of oil release in January 2000. It were quantified 38 compounds, parental and alkylated PAH, in 21 sediment samples collected in two surveys: 2000 and 2003. The PAH levels varied from 400 to 58439 ng g-1. Both tested techniques for origin identification of hydrocarbons have shown their applicability, being able to discriminate the PAH sources for the majority of the samples analysed. The bay sediments were separated into two big clusters: sediments with a clear pattern of petrogenic introduction of hydrocarbons (from intertidal area) and sediments with combustion characteristics (from subtidal region). Only a minority of the samples could not display a clear contribution of petrogenic or pyrolytic input. The diagnostic ratios that have exhibited high ability to distinguish combustion- and petroleum-derived PAH inputs for Guanabara Bay sediments were Phenanthrene+Anthracene/(Phenanthrene+Anthracene+C1Phenanthrene); Fluorantene/(Fluorantene+Pyrene); Σ (other 3-6 ring PAHs)/ Σ (5 alkylated PAH series). The PCA results prooved to be a useful tool for PAH source identification in the environment, corroborating the diagnostic indexes. In relation to the temporal evaluation carried out in this study, it was not verified significant changes on the class of predominant source of the samples. This result indicates that the hydrocarbons present in the Guanabara Bay sediments are mainly related to the long-term anthropogenic input and not directly related to acute events such as the oil spill of January 2000. This findings were similar to various international estuarine sites. Finally, this work had a complementary objective of evaluating the level of hydrocarbons exposure of the aquatic organisms of Guanabara Bay. It was a preliminary study in which a quantification of 12 individual biliar metabolites of PAH was performed in four demersal fish representing three different families. The analysed metabolites were 1-hydroxynaphtalene, 2-hidroxinaphtalene, 1hydroxyphenanthrene, 9-hydroxyphenanthrene, 2-hydroxyphenanthrene, 1hydroxypyrene, 3-hidroxibiphenil, 3- hydroxyphenanthrene, 1-hydroxychrysene, 9hydroxyfluorene, 4-hydroxyphenanthrene, 3-hydroxybenz(a)pyrene. The metabolites concentrations were found to be high, ranging from 13 to 177 µg g-1, however they were similar to worldwide regions under high anthropogenic input. Besides the metabolites established by the used protocol, it was possible to verified high concentrations of three other compounds not yet reported in the literature. They were related to pyrolytic PAH contribution to Guanabara Bay aquatic biota: 1-hydroxypyrine and 3-hydroxybenz(a)pyrine isomers

Mídia impressa e meio ambiente: um estudo da cobertura da mortandade de peixes no estuário do Rio Potengi, Natal-RN

Environmental issues are becoming increasingly habitual to the media, particularly when calamities are involved. A feature of environmental disasters is that they disclose both environmental crises and the media limitation in reporting them. The research tried to contribute to the subject through the study of press media coverage of a large fish kill along an estuary in the State of Rio Grande do Norte, Brazil, in 2007. Thematic content analysis of newspaper reports identified the predominance of a superficial and denunciative coverage, lacking an educational perspective, and with little contribution to formation of a critical sense in readers. It also has identified the precarious treatment of those scientific concepts that could substantiate the actual causes of mortality of tons of aquatic fauna

Valor nutritivo dos alimentos utilizados na formulação de rações para peixes tropicais

A seleção de ingredientes para a formulação de dietas para peixes requer o conhecimento prévio dos coeficientes de digestibilidade aparente da energia e nutrientes. O valor nutritivo de vários alimentos tem sido investigado no Brasil e o uso de fontes alternativas não deve resultar em efeitos negativos sobre o desempenho, saúde dos peixes e qualidade da água. Diversos fatores podem afetar a digestibilidade, sendo que os coeficientes de digestibilidade aparente dos ingredientes devem ser utilizados para a formulação de rações mais precisas e econômicas.

Karyotypic conservatism in samples of Characidium cf. zebra (Teleostei, Characiformes, Crenuchidae): physical mapping of ribosomal genes and natural triploidy

Basic and molecular cytogenetic analyses were performed in specimens of Characidium cf. zebra from five collection sites located throughout the Tietê, Paranapanema and Paraguay river basins. The diploid number in specimens from all samples was 2n = 50 with a karyotype composed of 32 metacentric and 18 submetacentric chromosomes in both males and females. Constitutive heterochromatin was present at the centromeric regions of all chromosomes and pair 23, had additional interstitial heterochromatic blocks on its long arms. The nucleolar organizer regions (NORs) were located on the long arms of pair 23, while the 5S rDNA sites were detected in different chromosomes among the studied samples. One specimen from the Alambari river was a natural triploid and had two extra chromosomes, resulting in 2n = 77. The remarkable karyotypic similarity among the specimens of C. cf. zebra suggests a close evolutionary relationship. on the other hand, the distinct patterns of 5S rDNA distribution may be the result of gene flow constraints during their evolutionary history.

Interspecific chromosomal divergences in the genus Characidium (Teleostei: Characiformes: Crenuchidae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Determinação de fósforo biodisponível em rações de peixes utilizando extração assistida por ultra-som e espectrofotometria no visível

The aim of the present work was to develop and optimize a method for determination of bioavailable phosphorus in samples of feces and fish feed using ultrasound extraction and subsequent quantification by visible spectrophotometry. Using as extractor solution HNO(3) 0.50 mol L(-1), the great conditions of extraction established were: sample mass - 100 mg, samples granulometry - < 60 mu m, sonification time - five cycles of 40 s and ultrasound potency - 136 W. The proposed method was applied in studies of digestibility of this nutrient in different feeds used in diets of juvenile of Nile tilapia.

Reprodução de peixes (Osteichthyes) em afluentes do reservatório de Volta Grande, Rio Grande, Sudeste do Brasil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Composition and structure of fish assemblage from Passa Cinco stream, Corumbataí river sub-basin, SP, Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Diversidade e ocorrência de peixes na área de influência da UHE Mogi Guaçu e lagoas marginais, bacia do alto rio Paraná, São Paulo, Brasil

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Alimentação de peixes na microbacia do Ribeirão Grande, Serra da Mantiqueira oriental, SP

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Distribuição de peixes na microbacia do Ribeirão Grande, Serra da Mantiqueira Oriental, São Paulo, Brasil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Peixes de riacho do parque estadual Morro do Diabo, Bacia do Alto Rio Paraná, SP

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Alimentação de peixes: proposta para análise de itens registrados em conteúdos gástricos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Integridade biótica de um córrego na bacia do Alto Rio Paraná avaliada por meio da comunidade de peixes

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)