Entorn de programació pel robot Stäubli mitjantçant MatLab, aplicació a un sistema de recol·lecció de peces basat en visió artificial

Els objectius del projecte són: realitzar un intèrpret de comandes en VAL3 que rebi les ordres a través d’una connexió TCP/IP; realitzar una toolbox de Matlab per enviar diferents ordres mitjançant una connexió TCP/IP; adquirir i processar mitjançant Matlab imatges de la càmera en temps real i detectar la posició d’objectes artificials mitjançant la segmentació per color i dissenyar i realitzar una aplicació amb Matlab que reculli peces detectades amb la càmera. L’abast del projecte inclou: l’estudi del llenguatge de programació VAL3 i disseny de l’ intèrpret de comandes, l’estudi de les llibreries de Matlab per comunicació mitjançant TCP/IP, per l’adquisició d’imatges, pel processament d’imatges i per la programació en C; el disseny de la aplicació recol·lectora de peces i la implementació de: un intèrpret de comandes en VAL3, la toolbox pel control del robot STAUBLI en Matlab i la aplicació recol·lectora de peces mitjançant el processament d’imatges en temps real també en Matlab

Desenvolupament d’una biblioteca per a la generació de diagrames vectorials basat en l’entorn gràfic de JAVA2D i aplicació d’aquesta biblioteca per a la implementació d’una eina per al desenvolupament de diagrames d’E/R

En aquest projecte es vol implementar una llibreria en Java, que proporcioni les eines necessàries per a crear diagrames i esquemes. Es vol proporcionar a la llibreria un conjunt d’objectes bàsics, també mètodes i funcionalitats suficients per a gestionar aquests objectes. Per altra part, es vol implementar una aplicació per a dissenyar diagrames Entitat-Relació que utilitzi aquesta llibreria

High Content Screening of a Kinase-Focused Library Reveals Compounds Broadly-Active against Dengue Viruses

Dengue virus is a mosquito-borne flavivirus that has a large impact in global health. It is considered as one of the medically important arboviruses, and developing a preventive or therapeutic solution remains a top priority in the medical and scientific community. Drug discovery programs for potential dengue antivirals have increased dramatically over the last decade, largely in part to the introduction of high-throughput assays. In this study, we have developed an image-based dengue high-throughput/high-content assay (HT/HCA) using an innovative computer vision approach to screen a kinase-focused library for anti-dengue compounds. Using this dengue HT/HCA, we identified a group of compounds with a 4-(1-aminoethyl)-N-methylthiazol-2-amine as a common core structure that inhibits dengue viral infection in a human liver-derived cell line (Huh-7.5 cells). Compounds CND1201, CND1203 and CND1243 exhibited strong antiviral activities against all four dengue serotypes. Plaque reduction and time-of-addition assays suggests that these compounds interfere with the late stage of viral infection cycle. These findings demonstrate that our image-based dengue HT/HCA is a reliable tool that can be used to screen various chemical libraries for potential dengue antiviral candidates. © 2013 Cruz et al.

Intermolecular interactions of the malate synthase of Paracoccidioides spp

Background: The fungus Paracoccidioides spp is the agent of paracoccidioidomycosis (PCM), a pulmonary mycosis acquired by the inhalation of fungal propagules. Paracoccidioides malate synthase (PbMLS) is important in the infectious process of Paracoccidioides spp because the transcript is up-regulated during the transition from mycelium to yeast and in yeast cells during phagocytosis by murine macrophages. In addition, PbMLS acts as an adhesin in Paracoccidioides spp. The evidence for the multifunctionality of PbMLS indicates that it could interact with other proteins from the fungus and host. The objective of this study was to identify and analyze proteins that possibly bind to PbMLS (PbMLS-interacting proteins) because protein interactions are intrinsic to cell processes, and it might be possible to infer the function of a protein through the identification of its ligands. Results: The search for interactions was performed using an in vivo assay with a two-hybrid library constructed in S. cerevisiae; the transcripts were sequenced and identified. In addition, an in vitro assay using pull-down GST methodology with different protein extracts (yeast, mycelium, yeast-secreted proteins and macrophage) was performed, and the resulting interactions were identified by mass spectrometry (MS). Some of the protein interactions were confirmed by Far-Western blotting using specific antibodies, and the interaction of PbMLS with macrophages was validated by indirect immunofluorescence and confocal microscopy. In silico analysis using molecular modeling, dynamics and docking identified the amino acids that were involved in the interactions between PbMLS and PbMLS-interacting proteins. Finally, the interactions were visualized graphically using Osprey software. Conclusion: These observations indicate that PbMLS interacts with proteins that are in different functional categories, such as cellular transport, protein biosynthesis, modification and degradation of proteins and signal transduction. These data suggest that PbMLS could play different roles in the fungal cell. © 2013 de Oliveira et al.; licensee BioMed Central Ltd.

Investigação por eletroresistividade de ambientes cársticos no município de São João de Pirabas - PA

A presente tese procura investigar ambientes cársticos, usando os métodos eletroresistivos de sondagem elétrica vertical e caminhamento dipolo-dipolo. A área piloto para essa investigação está localizada na porção nordeste do Estado do Pará, no Município de São João de Pirabas. Nessa localidade, durante a perfuração de um poço para abastecimento de água para a cidade, a equipe de prospecção da Fundação Nacional de Saúde encontrou problemas devido a presença de cavidades nos calcários da Formação Pirabas. Para analisar esse problema foram realizadas 15 sondagens elétricas verticais, usando o arranjo Schlumberger, em diversos pontos da cidade. A abertura máxima dos eletrodos de corrente, para a realização das sondagens, foi em torno de 600 metros. As curvas de resistividade aparente das sondagens foram, inicialmente, interpretadas usando programas computacionais que calculam as resistividade do meio investigado com modelos unidimensionais, sem variações laterais de resistividade. Porém, as sondagens realizadas próximas ao poço, onde ocorrem cavernas, foram interpretadas usando modelos bidimensionais para representar as variações laterais de resistividade devido às cavernas. O algoritmo usado para essa interpretação se baseia na técnica dos elementos finitos. Os resultados das interpretações das sondagens elétricas, para os vários modelos de cavernas estudados, mostraram que as anomalias de resistividade devido a presença de cavernas são muito pequenas. Devido a este fato, o método de sondagem elétrica vertical não apresentou boa resolução para identificar com precisão a presença deste tipo de caverna em subsuperfície. Por outro lado, os resultados numéricos obtidos pelo caminhamento dipolo-dipolo mostraram que esse método é mais eficiente e menos ambíguo, para estudar cavernas, que o método de sondagem elétrica vertical. Contudo o método de caminhamento dipolo-dipolo é muito mais dispendioso do que o de sondagem elétrica verticais, tanto do ponto de vista de trabalho de campo como no processamento e interpretação de dados.

An experiment on a unified control construct /

Bibliography: p. 34.

SPORRT; simplified polynomial root finding at a remote terminal.

"Supported in part by ... Grant no. US NSF GP-9665."

GIML reference manual /

"Supported in part by the Energy Research and Development Administration under contract US ERDA-EY-76-S-02-2383."

Arrays in PL/I : a lesson for PLATO /

Thesis (M.S.)-University of Illinois at Urbana-Champaign.

Floyd productions for ALGOL-60* /

"*This work was supported in part by the U.S. Atomic Energy Commission under contract AT(11-1)-1018."

An algorithm for evaluating array expressions in OL/2 /

"January, 1971."

A generalized assembler.

Issued also as thesis (M.S.) University of Illinois.

Lisp, a CAI implementation /

Bibliography: p. 29.

A multiprocessor for simulation applications.

Vita.

plldo, the DO loop in PL/1 : a lesson for PLATO IV /

Bibliography: p. 28.