Biostratigraphical investigations of Lago d'Averno, near Naples

Remains of diatoms, molluscs, ostracods, foraminifera and pollen exines preserved in the sediments of Lago d'Averno, a volcanic lake in the Phlegrean Fields west of Naples, allowed us to reconstruct the changes in the ecological conditions of the lake and of the vegetation around it for the period from 800 BC to 800 AD. Lago d'Averno was at first a freshwater lake, temporarily influenced by volcanic springs. Salinity increased slowly during Greek times as a result of subsidence of the surrounding land. Saline conditions developed only after the lake was connected with the sea by a canal, when Portus Julius was built in 37 BC. The first post-Roman period of uplift ended with a short freshwater phase during the 7th century after Christ. Deciduous oakwoods around the lake was transformed into a forest of evergreen oaks in Greek times and thrived there - apparently almost uninfluenced by man - until it was felled, when the Avernus was incorporated into the new Roman harbour in 37 BC, to construct a shipyard and other military buildings there. Land-use was never more intense than during Roman times and weakest in Greek and Early Roman times, when the Avernus was considered a holy place, the entrance to the underworld.

The effect of boundary conditions in the numerical solution of 3-D thermoelastic problems

After the extensive research on the capabilities of the Boundary Integral Equation Method produced during the past years the versatility of its applications has been well founded. Maybe the years to come will see the in-depth analysis of several conflictive points, for example, adaptive integration, solution of the system of equations, etc. This line is clear in academic research. In this paper we comment on the incidence of the manner of imposing the boundary conditions in 3-D coupled problems. Here the effects are particularly magnified: in the first place by the simple model used (constant elements) and secondly by the process of solution, i.e. first a potential problem is solved and then the results are used as data for an elasticity problem. The errors add to both processes and small disturbances, unimportant in separated problems, can produce serious errors in the final results. The specific problem we have chosen is especially interesting. Although more general cases (i.e. transient)can be treated, here the domain integrals can be converted into boundary ones and the influence of the manner in which boundary conditions are applied will reflect the whole importance of the problem.

Some consistent finite element formulations of 1-D beam models: a comparative study

A consistent Finite Element formulation was developed for four classical 1-D beam models. This formulation is based upon the solution of the homogeneous differential equation (or equations) associated with each model. Results such as the shape functions, stiffness matrices and consistent force vectors for the constant section beam were found. Some of these results were compared with the corresponding ones obtained by the standard Finite Element Method (i.e. using polynomial expansions for the field variables). Some of the difficulties reported in the literature concerning some of these models may be avoided by this technique and some numerical sensitivity analysis on this subject are presented.

Random mutagenesis of Thermus aquaticus DNA polymerase I: concordance of immutable sites in vivo with the crystal structure.

Expression of Thermus aquaticus (Taq) DNA polymerase I (pol I) in Escherichia, coli complements the growth defect caused by a temperature-sensitive mutation in the host pol I. We replaced the nucleotide sequence encoding amino acids 659-671 of the O-helix of Taq DNA pol I, corresponding to the substrate binding site, with an oligonucleotide containing random nucleotides. Functional Taq pol I mutants were selected based on colony formation at the nonpermissive temperature. By using a library with 9% random substitutions at each of 39 positions, we identified 61 active Taq pol I mutants, each of which contained from one to four amino acid substitutions. Some amino acids, such as alanine-661 and threonine-664, were tolerant of several or even many diverse replacements. In contrast, no replacements or only conservative replacements were identified at arginine-659, lysine-663, and tyrosine-671. By using a library with totally random nucleotides at five different codons (arginine-659, arginine-660, lysine-663, phenylalanine-667, and glycine-668), we confirmed that arginine-659 and lysine-663 were immutable, and observed that only tyrosine substituted for phenylalanine-667. The two immutable residues and the two residues that tolerate only highly conservative replacements lie on the side of O-helix facing the incoming deoxynucleoside triphosphate, as determined by x-ray analysis. Thus, we offer a new approach to assess concordance of the active conformation of an enzyme, as interpreted from the crystal structure, with the active conformation inferred from in vivo function.

Res. of E.D. Lay, Esq., Sec 2, Ypsilanti Tp. Mich.

Ypsilanti Township (Mich.) residence. Publication information: Chicago, Ill. : Everts & Stewart, 1874.

An investigation of truck size and weight limits - technical supplement. Volume I: analysis of truck payloads under various limits of size, weight and configuration. Final report.

Transportation Department, Office of the Assistant Secretary for Policy and International Affairs, Washington, D.C.

Methods for reducing large speed differences in traffic streams. Volume I - inventory of methods. Task A report.

Federal Highway Administration, Office of Safety and Traffic Operations Research and Development, Washington, D.C.

The picture gallery of Charles I.

Mode of access: Internet.

Research input for computer simulation of automobile collisions. Volume I: development of data bank. Final report.

National Highway Traffic Safety Administration, Washington, D.C.

Guidelines for permitting overloads. Part I: effect of overloaded vehicles on the Indiana highway network. Final report.

Indiana Department of Transportation, Indianapolis

Impact study on driving by special populations, final report. Volume I - conduct of the project and state of the art.

National Highway Traffic Safety Administration, Washington, D.C.

Driver's visibility requirements for roadway delineation. Volume I: effects of contrast and configuration on driver performance and behavior. Final report.

Federal Highway Administration, Washington, D.C.