No caminho das artes marciais: a relação mestre e discípulo como educação sensível

This research reflects the relationship between Master and Disciple original from the Martial Arts, and anchors their focus on sensitive education that emerges from this relationship. The interest here is knowing how the tradition of millenarian teachings is passed through the years, and how it gives from the relationship of Master and Disciple. To that end, I lean me in that context and also reflect on my experience as a Disciple of the Martial Arts, to that end, I lean me in that context and also reflect on my experience as a Disciple of the Martial Arts, and is from the immemorial fund that can give voice to that experience, through my body attached in the world of significations in which the experience lived is narrated by the story. Anchored from the phenomenological attitude from the philosopher french Maurice Merleau-Ponty, I think this research on three central pillars to guide our study categories, namely: the lived experience, body and liberty. Still, as a form of highlighting this sensitive work, beyond the texts of the philosopher Merleau-Ponty, we bring our dialogue of the cinema, literature and the writings of some the Masters of Martial Arts. For that, I think this research as a journey, where it, Master and Disciple march together in the ways of Martial Arts, baptizing and celebrating this sensitive education from that relationship affective and empathic

Erythrocyte ghost cell-alkaline phosphatase: construction and characterization of a vesicular system for use in biomineralization studies

Alkaline phosphatase is required for the mineralization of bone and cartilage. This enzyme is localized in the matrix vesicle, which plays a role key in calcifying cartilage. In this paper we standardize a method to construction a resealed ghost cell-alkaline phosphatase system to mimic matrix vesicles and examine the kinetic behavior of the incorporated enzyme. Polidocanol-solubilized alkaline phosphatase, free of detergent, was incorporated into resealed ghost cells. This process was time-dependent and practically 50% of the enzyme was incorporated into the vesicles in 40 h of incubation, at 25 degreesC. Alkaline phosphatase-ghost cell systems were relatively homogeneous with diameters of about 300 nm and were more stable when stored at -20 degreesC.Alkaline phosphatase was completely released from the resealed ghost cell-system using only phospholipase C. These experiments confirm that the interaction between alkaline phosphatase and the lipid bilayer of resealed ghost cell is exclusively via glycosylphosphatidylinositol (GPI) anchor of the enzyme.An important point shown is that an enzyme bound to resealed ghost cell does not lose the ability to hydrolyze ATP, pyrophosphate and p-nitrophenyl phosphate (PNPP), but the presence of a ghost membrane, as a support of the enzyme, affects its kinetic properties. Moreover, calcium ions stimulate and phosphate ions inhibit the PNPPase activity of alkaline phosphatase present in resealed ghost cells. (C) 2002 Elsevier B.V. B.V. All rights reserved.

Avaliação do desempenho de uma cortina de estacas espaçadas, atirantada, em areia

It is presented the analysis of a retaining wall designed for the basement of a residential building, located in Natal/RN, which consists in a spaced pile wall, anchored by tiebacks, in sand. This structure was instrumented in order to measure the wall s horizontal movements and the load distribution throughout the anchor fixed length. The horizontal movements were measured with an inclinometer, and the loads in the anchors were measured with strain gages, installed in three places throughout the anchor fixed length. Measurements for displacement were done right after the implementation of each stage of the building and right after the conclusion of the building, and the measurements for loads in the anchors were done during the performance test, at the moment of the locking off and, also, right after the conclusion of the building. From the data of displacement were obtained velocity and acceleration data of wall. It was found that the time elapsed on braced installation was decisive in the magnitude of the displacements. The maximum horizontal displacement of wall ranged between 0,18 and 0,66% of the final depth of excavation. The loads in the anchors strongly reduced to approximately half the anchor fixed length, followed an exponential distribution. Furthermore, it was found that there was a loss of load in the anchors over time, reaching 50% loss in one of them

Letramento no trânsito: eventos e práticas na formação de condutores de veículos

In the contemporary society, the language is presented in all social spaces and assumes many different purposes in order to meet the needs that emerge from each of these sphere. In traffic, this reality is not different. To guide vehicles, it is necessary to know, by means of reading artifacts, what the legislation establishes in what concerns the way to act in this domain. Thus, this works aims at describing the practices of literacy held in events of driver trainings and know the expectations generated by drivers/learners from this training. In theoretical terms, it anchors in Literacy Studies, comprehended here as social practices (BARTON; HAMILTON, 1998; KLEIMAN, 1995, 2008; MORTATTI, 2004; STREET, 1984; OLIVEIRA, 2008, 2010; ROJO, 2009; PAZ, 2008). Genre Theory (BRONCKART, 2004, 1999; OLIVEIRA, 2010) and in your multimodal instance (KRESS; VAN LEEUWEN, 1996; DIONÍSIO, 2006). In terms of methodology, it follows the bias of qualitative research, because of its ethnographic nature (BOGDAN; BIKLEN, 1994; MINAYO, 2010; CANÇADO, 1994; CHIZZOTTI, 2005). The research corpus was generated by reading the Brazilian Traffic Code, by observing the literacy events held in Drivers Training Centers of Natal, analysis of course books used in these events, plus questionnaires with open and closed questions and semistructured interviews. The collaborators are constituted of drivers in training, and instructors who work in this field. The analyses show significant contributions regarding the placement more committed of future drivers with the welfare and safety of those who use the public roads, from the practice or reading done during the traffic training. The contribution of this work lies in the possibility to expand the discussion about the language practice uses regarding the training for the traffic, more specifically, the training of drivers of vehicles

Short-Term Effects of the Use of Non-Rigid Tools for Postural Control by Adults with Intellectual Disabilities

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Developing an Anchor system to enhance postural control

This article uses an anchor metaphor to explain the dynamic interplay between the human body's active uses of nonrigid tools to mediate information about its adjacent environment to enhance postural control. The author used an anchor system (ropes attached to varying weights resting on the floor) to test blindfolded adults who performed a restricted-balance task (30 s one-foot standing). Participants were tested while holding the anchors under a variety of weight conditions (125 g, 250 g, 500 g, and I kg) and again during a baseline condition (no anchors). When compared with the baseline condition, there was a significant reduction in the amount of body sway across the anchor conditions. The author found that mechanical support provided by the anchor system was secondary to its haptic exploratory function and that an individual can use the anchoring strategy with a dual purpose: for resting and for reorientation after intrinsic disruptions.

Kinetic characterization of hypophosphatasia mutations with physiological substrates

We have analyzed 16 missense mutations of the tissue-nonspecific AP (TNAP) gene found in patients with hypophosphatasia. These mutations span the phenotypic spectrum of the disease, from the lethal perinatal/infantile forms to the less severe adult and odontohypophosphatasia. Site-directed mutagenesis was used to introduce a sequence tag into the TNAP cDNA and eliminate the glycosylphosphatidylinositol (GPI)-anchor recognition sequence to produce a secreted epitope-tagged TNAP (setTNAP). The properties of GPI-anchored TNAP (gpiTNAP) and setTNAP were found comparable. After introducing each single hypophosphatasia mutation, the setTNAP and mutant TNAP cDNAs were expressed in COS-1 cells and the recombinant flagged enzymes were affinity purified. We characterized the kinetic behavior, inhibition, and heat stability properties of each mutant using the artificial substrate p-nitrophenylphosphate (pNPP) at pH 9.8. We also determined the ability of the mutants to metabolize two natural substrates of TNAP, that is, pyridoxal-5'-phosphate (PLP) and inorganic pyrophosphate (PPi), at physiological pH. Six of the mutant enzymes were completely devoid of catalytic activity (R54C, R54P, A94T, R206W, G317D, and V365I), and 10 others (A16V, A115V, A160T, A162T, E174K, E174G, D277A, E281K, D361V, and G439R) showed various levels of residual activity. The A160T substitution was found to decrease the catalytic efficiency of the mutant enzyme toward pNPP to retain normal activity toward PPi and to display increased activity toward PLP. The A162T substitution caused a considerable reduction in the pNPPase, PPiase, and PLPase activities of the mutant enzyme. The D277A mutant was found to maintain high catalytic efficiency toward pNPP as substrate but not against PLP or PPi. Three mutations ( E174G, E174K, and E281K) were found to retain normal or slightly subnormal catalytic efficiency toward pNPP and PPi but not against PLP. Because abnormalities in PLP metabolism have been shown to cause epileptic seizures in mice null for the TNAP gene, these kinetic data help explain the variable expressivity of epileptic seizures in hypophosphatasia patients.

Porous titanium scaffolds produced by powder metallurgy for biomedical applications

Porous titanium scaffolds are promising materials for biomedical applications such as prosthetic anchors, fillers and bone reconstruction. This study evaluated the bone/titanium interface of scaffolds with interconnected pores prepared by powder metallurgy, using scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS). Porous scaffolds and dense samples were implanted in the tibia of rabbits, which were subsequently killed 1, 4, and 8 weeks after surgery. Initial bone neoformation was observed one week after implantation. Bone ingrowth in pores and the Ca/P ratio at the interface were remarkably enhanced at 4 and 8 weeks. The results showed that the interconnected pores of the titanium scaffolds promoted bone ingrowth, which increased over time. The powder metallurgy technique thus proved effective in producing porous scaffolds and dense titanium for biomedical applications, allowing for adequate control of pore size and porosity and promoting bone ingrowth.

Argentine convertibility: is it a relevant precedent for the dollarization process in Ecuador?

Includes bibliography

Physical factors affecting plasmid DNA compaction in stearylamine-containing nanoemulsions intended for gene delivery

Cationic lipids have been used in the development of non-viral gene delivery systems as lipoplexes. Stearylamine, a cationic lipid that presents a primary amine group when in solution, is able to compact genetic material by electrostatic interactions. In dispersed systems such as nanoemulsions this lipid anchors on the oil/water interface confering a positive charge to them. The aim of this work was to evaluate factors that influence DNA compaction in cationic nanoemulsions containing stearylamine. The influence of the stearylamine incorporation phase (water or oil), time of complexation, and different incubation temperatures were studied. The complexation rate was assessed by electrophoresis migration on agarose gel 0.7%, and nanoemulsion and lipoplex characterization was done by Dynamic Light Scattering (DLS). The results demonstrate that the best DNA compaction process occurs after 120 min of complexation, at low temperature (4 ± 1 °C), and after incorporation of the cationic lipid into the aqueous phase. Although the zeta potential of lipoplexes was lower than the results found for basic nanoemulsions, the granulometry did not change. Moreover, it was demonstrated that lipoplexes are suitable vehicles for gene delivery. © 2012 by the authors; licensee MDPI, Basel, Switzerland.

Tornando-me professora de ciências com alunos indíginas Kaiowá e Guarani

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Representações sociais de jovens-alunos de uma escola ribeirinha sobre exploração sexual juvenil nas balsas do Marajó e as implicações nas suas escolarizações

Este estudo teve como contexto uma escola ribeirinha no município de Breves, situado na Ilha do Marajó, no Estado do Pará. Objetivo mestre: Analisar as representações sociais de jovens-alunos do ensino fundamental da Escola Municipal de Ensino Fundamental São Francisco, da Comunidade São Francisco, no município de Breves sobre exploração sexual nas balsas do Marajó e as implicações nas suas vidas escolares. Objetivos condutores: Identificar o perfil dos alunos-jovens; Verificar as imagens e os significados destes alunos-jovens sobre a exploração sexual juvenil; Destacar as objetivações e as ancoragens como elementos que compõem as Representações Sociais de alunos-jovens e alunas-jovens sobre a exploração sexual juvenil; Relacionar as Representações Sociais de alunos e alunas jovens sobre a exploração sexual juvenil nas balsas do Marajó e as implicações nas suas vidas escolares. Participaram deste estudo 16 jovens, na faixa etária entre 14 e 20 anos, matriculados entre a 4ª e 6ª série do ensino fundamental. Tratou-se de uma pesquisa qualitativa-descritiva. O referencial teórico foi a Teoria das Representações Sociais – TRS, com base em Moscovici (2010) e Nascimento (2002). A análise de dados se deu a partir dos referenciais metodológicos de Franco (2008) e Nascimento e Moraes (2010); As Representações Sociais dos jovens-alunos sobre a exploração sexual juvenil nas balsas do Marajó constituíram-se no Rio de possibilidades, cuja imagem se apresenta pelo encantamento, e Rio que traga, imagem representada pelo desencantamento. Além destas RS, destacaram-se significações cuja imagem foi o sentimento de culpa, que se destaca como significado de ser julgado pela comunidade, ter a atribuição de balseira, medo de sofrer ameaças e pelo sentimento de ausência do poder público. A partir destas significações, ressaltamos enquanto ponto de ancoragem o silêncio. Este sentimento que não se pode ouvir brada de sentidos, ora pelo medo de sofrer alguma espécie de julgamento social ora pelo sentimento de esvaziamento que esta condição da exploração sexual se materializa em suas vivências. As implicações escolares constituídas a partir das Representações Sociais de jovens-alunos sobre a exploração sexual juvenil foram: fragilização na formação escolar e no processo psicossocial de desenvolvimento juvenil; falta de aprendizagem; desatenção nos conteúdos e atividades escolares; desinteresse pela escolarização (Abandono escolar e Repetência escolar). Estes elementos e as análises teóricas articuladas a partir dessas Representações Sociais nos apontam como conclusão que a exploração sexual nos rios e furos da paisagem amazônica ainda é pouco visível, uma vez que as ações do poder público ainda não dão o lugar para este fenômeno no sentido de seu combate e enfrentamento. Entendemos, portanto, que deve haver uma ação educativa que possibilite o enfrentamento da exploração sexual juvenil.

Representações sociais de jovens da ilha de Cotijuba – Belém (PA) sobre o ensino médio e as relações com seus projetos de vida

Este estudo enfoca as Representações Sociais (RS) de jovens da ilha de Cotijuba, Belém/PA, sobre o Ensino Médio e as relações desse ensino com seus projetos de vida. O objetivo geral dessa investigação foi analisar as Representações Sociais (RS) de jovens estudantes do Ensino Médio em Cotijuba, sobre o Ensino Médio e as relações deste ensino com seus projetos de vida. Os objetivos específicos foram: caracterizar o perfil sociocultural dos jovens sujeitos da pesquisa; identificar as objetivações e ancoragens constitutivas das RS dos jovens sobre o Ensino Médio; conhecer os projetos de vida dos jovens; indicar as relações entre Ensino Médio e os projetos de vida dos jovens. A Teoria das Representações Sociais embasa este estudo, cujos procedimentos de análise utilizados a partir dessa teoria foram a objetivação e a ancoragem para caracterizar as RS. O lócus de pesquisa foi a Escola Estadual de Ensino Fundamental e Médio Profª Marta da Conceição, situada na ilha de Cotijuba, Belém (PA). Os sujeitos desse estudo foram jovens estudantes do 3º ano do Ensino Médio dessa escola. Na pesquisa de campo foram utilizados questionários para caracterizar o perfil da escola e dos alunos. Houve ainda uma roda de conversa com os sujeitos do estudo. A Análise do Discurso possibilitou avaliar o conteúdo das informações geradas a partir dos questionários e da roda de conversa para caracterizar as RS. As Representações Sociais dos jovens ilhéus de Cotijuba revelaram que o Ensino Médio representa uma conquista para esses sujeitos. Por outro lado, as RS dos jovens indicam que as dificuldades enfrentadas no processo de ensino e a pouca articulação do currículo com o contexto de vivência possibilitaram uma dissociação entre o Ensino Médio e projetos de vida.

Representações sociais de adolescentes: ato infracional e projeto de vida

Este estudo tem por objeto as representações sociais das adolescentes autoras de atos infracionais sobre a socioeducação e as implicações nos seus projetos de vida. A problemática se orientou com o foco nas representações sociais anunciadas pelas adolescentes autoras de atos infracionais sobre as medidas socioeducativas de internação e semiliberdade e as implicações nos seus projetos de vida. O lócus da pesquisa foi o Centro Socioeducativo Feminino - CESEF que pertence a Fundação de Atendimento Socioeducativo do Estado do Pará. Os objetivos do estudo foram: a) identificar o perfil das adolescentes custodiadas no CESEF, na faixa etária de 13 a 18 anos incompletos em cumprimento de medida socioeducativa de internação e semiliberdade; b) caracterizar as imagens e sentidos que atribuem às medidas socioeducativas que cumprem; c) destacar as objetivações e as ancoragens que organizam as representações sociais que as adolescentes possuem sobre as medidas que cumprem; d) compreender o projeto de vida das adolescentes; e) correlacionar as representações sociais das adolescentes sobre as medidas com os seus projetos de vida. A metodologia seguiu a abordagem qualitativa e o referencial teórico centrou-se na Teoria das Representações Sociais de Moscovici (1978) e Jodelet (2001). Os aportes teóricos de Nascimento, 2002; 2006 e 2011; e Melucci, 1996 nos auxiliaram nas incursões e articulações sobre a adolescência. Os referenciais de Volpi, 2001; Louro, 1998; Saffioti, 1987; Assis e Constantino, 2001; Zaluar, 1994; Voegeli, 2003 no entendimento de gênero e no envolvimento de mulheres em práticas delitivas. Rizzini e Pilotti, 2011; Marcílio, 1996; 2002; Oliveira e Assis, 1999; sobre o histórico das políticas de atendimento à adolescência autora de ato Infracional no Brasil e no Pará. Os sujeitos de estudo foram seis adolescentes do Centro Socioeducativo Feminino em cumprimento das medidas socioeducativas de internação e semiliberdade. O levantamento de dados constou de: entrevistas com roteiro semi estruturado; observação in loco e diário de campo. Os instrumentos para a análise dos dados utilizamos a análise de conteúdo de acordo com a abordagem proposta por Franco (2003). Os resultados revelam que as representações sociais das adolescentes infratoras sobre a socioeducação e as implicações nos seus projetos de vida se constituem em torno da medida disciplinar. Que por sua vez se consolidou ao longo do processo histórico das medidas socioeducativas. O sentido que as adolescentes produzem sobre estas medidas ancoram na dúvida quanto a sua eficiência. A imagem consensual que elaboram sobre as suas vidas após o cumprimento dessas medias assinalam que pode haver retorno a instituição socioeducativa por infringirem as leis. Assim estas representações sociais comprometem os projetos de vida das adolescentes autoras de atos infracionais.

Construção e ancoragem ontológica do vocabulário básico bilíngë do turismo para fins didáticos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)