Analysis of genetic instability during mammary tumor progression using a novel selection-based assay for in vivo mutations in a bacteriophage lambda transgene target.

Genetic instability is thought to be responsible for the numerous genotypic changes that occur during neoplastic transformation and metastatic progression. To explore the role of genetic instability at the level of point mutations during mammary tumor development and malignant progression, we combined transgenic mouse models of mutagenesis detection and oncogenesis. Bitransgenic mice were generated that carried both a bacteriophage lambda transgene to assay mutagenesis and a polyomavirus middle T oncogene, mammary gland-targeted expression of which led to metastatic mammary adenocarcinomas. We developed a novel assay for the detection of mutations in the lambda transgene that selects for phage containing forward mutations only in the lambda cII gene, using an hfl- bacterial host. In addition to the relative ease of direct selection, the sensitivity of this assay for both spontaneous and chemically induced mutations was comparable to the widely used mutational target gene, lambda lacI, making the cII assay an attractive alternative for mutant phage recovery for any lambda-based mouse mutagenesis assay system. The frequencies of lambda cII- mutants were not significantly different in normal mammary epithelium, primary mammary adenocarcinomas, and pulmonary metastases. The cII mutational spectra in these tissues consisted mostly of G/C-->A/T transitions, a large fraction of which occurred at CpG dinucleotides. These data suggest that, in this middle T oncogene model of mammary tumor progression, a significant increase in mutagenesis is not required for tumor development or for metastatic progression.

Rock 314: A Novel

During this Capstone project, the author composed and revised the introductory chapters of the novel Rock 314. This Capstone is comprised of the first four of those chapters and also includes a reflective essay that explores the many influences and inspirations that led to its creation. Rock 314 is a story about a lonely young artist who is unexpectedly taken away from the world he knows and finds himself flung across the cosmos in search of his kidnapped grandfather. This novel uses humor as a literary device to satirize contemporary society. In addition to an explanation of influences and story conception, the essay includes all cognitive decisions and discoveries made by the author throughout the evolution of this project.

Essay on the French Revolution and the Napoleonic Wars,

This is a contemporary draft composition reviewing the course of the French Revolution and Napoleonic Wars. Tudor offers opinions on England’s diplomatic options after the renewal of hostilities and Prime Minister William Pitt the Younger: "His task is momentous and all Europe are watching his movements with anxiety." The document is signed "Marcus Valinus."

Anatomy improv'd and illustrated with regard to the uses thereof in designing : not only laid down from an examen of the bones and muscles of the human body, but also demonstrated and exemplified from the most celebrated antique statues in Rome : exhibited in a great number of copper plates, with all the figures in various views : intended originally for ye use of the Royal French Academy of Painting and Sculpture /

Re-engraved by Michael van der Gucht and others after the original edition published at Rome in 1691.

Contemporary France,

Vols. 2-4 have imprint: London, A Constable & co. ltd.

Cousin Geoffrey, the old bachelor. A novel. To which is added Claude Stocq.

"Claude Stocq: a tale of the sixteenth century. From the French" (v. 3, p. [135]-323) not by the same author. cf. Advertisement, v. 1.

Contemporary thought in France, a seen in her literature from 1900 to 1914.

Bibliography: p. 29-30.

Tradition and change : studies in contemporary literature /

Includes index.

The Napoleon anecdotes : illustrating the mental energies of the late Emperor of France, and the character and actions of his contemporary statesmen and warriors /

Mode of access: Internet.

Elfrida, or Paternal ambition : a novel in two volumes /

Manuscript signature of J. Harman appears in pencil or ink in each volume on page preceding t.p. and first page of text.

The Huguenot : a tale of the French Protestants /

In blue cloth with white paper spine labels.

The Napoleon anecdotes : illustrating the mental energies of the late emperor of France; and the characters and actions of his contemporary statesmen and warriors /

Mode of access: Internet.

The memoirs of François René, vicomte de Chateaubriand, sometime ambassador to England : being a translation by Alexander Teixeira de Mattos of the Mémoires d'outre-tombe, with illustrations from contemporary sources.

Includes bibliographical references and index.

Napoleon and the Queen of Prussia; an historical novel,

Tr. of Napoleon und konigen Louise.

The origins of contemporary France

Includes bibliographical references and index.