Signal Processing Techniques for Chipless UWB RFID Thermal Threshold Detector Detection.

This letter presents signal processing techniques to detect a passive thermal threshold detector based on a chipless time-domain ultrawideband (UWB) radio frequency identification (RFID) tag. The tag is composed by a UWB antenna connected to a transmission line, in turn loaded with a biomorphic thermal switch. The working principle consists of detecting the impedance change of the thermal switch. This change occurs when the temperature exceeds a threshold. A UWB radar is used as the reader. The difference between the actual time sample and a reference signal obtained from the averaging of previous samples is used to determine the switch transition and to mitigate the interferences derived from clutter reflections. A gain compensation function is applied to equalize the attenuation due to propagation loss. An improved method based on the continuous wavelet transform with Morlet wavelet is used to overcome detection problems associated to a low signal-to-noise ratio at the receiver. The average delay profile is used to detect the tag delay. Experimental measurements up to 5 m are obtained.

Riconoscimento delle emozioni tramite strumentazione per EEG

Lo scopo di questa trattazione è quindi di illustrare il lavoro svolto nel tentativo di classificare le reazioni emozionali ad immagini con una forte carica emozionale, sia positiva che negativa. A tale scopo sono stati acquisiti i segnali EEG di diversi soggetti durante l’esposizione ad immagini di vario contenuto, insieme alla loro reazione dichiarata alle immagini stesse. Queste sono state immagazzinate, elaborate utilizzando diversi metodi di estrazione delle informazioni, ed infine si è tentato di effettuare un riconoscimento di pattern sui segnali tramite algoritmi di apprendimento supervisionato; i dati sono stati quindi divisi tra dati di “training”, utilizzati per la strutturazione dell’algoritmo, e dati di test, necessari per la verifica dell’affidabilità dell’algoritmo.

Use of a novel DNA melting profile assay for the identification of PCR-amplified genomic sequences encoding for variant-specific surface proteins from the clonal GS/M-83-H7 line of Giardia lamblia.

During infections, Giardia lamblia undergoes a continuous change of its major surface antigens, the variant-specific surface proteins (VSPs). Many studies on antigenic variation have been performed using G. lamblia clone GS/M-83-H7, which expresses surface antigen VSP H7. The present study was focused on the identification and characterization of vsp gene sequences within the genome of the clonal G. lamblia GS/M-83-H7 line. For this purpose, we applied a PCR which specifically amplified truncated sequences from the 3'-terminal region of the vsp genes. Upon cloning, most of the vsp gene amplification products were shown to be approximately identical in size and thus could not be distinguished from each other by conventional gel electrophoresis. In order to pre-estimate the sequence complexity within the large panel of vsp clones isolated, we elaborated a novel concept which facilitated our large-scale genetic screening approach: PCR products from cloned DNA molecules were generated and then subjected to a DNA melting profile assay based on the use of the LightCycler Instrument. This high-throughput assay system proved to be well suited to monitor sequence differences between the amplification products from closely related vsp genes and thus could be used for the primary, sequence-related discrimination of the corresponding clones. After testing 50 candidates, vsp clones could be divided into five groups, each characterized by an individual DNA melting profile of the corresponding amplification products. Sequence analysis of some of these 50 candidates confirmed data from the aforementioned assay in that clones were demonstrated to be identical within, but different between, the distinct groups. The nucleotide and deduced amino acid sequences of five representative vsp clones showed high similarities both among each other and also with the corresponding gene segment of the variant-specific surface antigen (VSP H7) expressed by the original GS/M-83-H7 variant type. Furthermore, three of the genomic vsp sequences turned out to be identical to vsp sequences that represented previously characterized transcription products from in vivo- or in vitro-switched GS/M-83-H7 trophozoites. In conclusion, the DNA melting profile assay seems to be a versatile tool for the PCR-based genotyping of moderately or highly diversified sequence orthologues.

Upward muon through IMB detector

IMB (Irvine, Michigan, Brookline), a collaboration between the University of Michigan, the University of California at Irvine, and the U.S. Department of Energy, was an experiment designed to determine the ultimate stability of matter. A high energy muon, created by a neutrino interaction in the earth below the detector, enters the bottom and exits the top. The slashes are the PMT [photomultiplier tubes] hits and the purple line is the estimated path of the muon.

Regression analysis of ranked segment parameters for optic nerve head classification:a pilot study

Purpose: To determine whether curve-fitting analysis of the ranked segment distributions of topographic optic nerve head (ONH) parameters, derived using the Heidelberg Retina Tomograph (HRT), provide a more effective statistical descriptor to differentiate the normal from the glaucomatous ONH. Methods: The sample comprised of 22 normal control subjects (mean age 66.9 years; S.D. 7.8) and 22 glaucoma patients (mean age 72.1 years; S.D. 6.9) confirmed by reproducible visual field defects on the Humphrey Field Analyser. Three 10°-images of the ONH were obtained using the HRT. The mean topography image was determined and the HRT software was used to calculate the rim volume, rim area to disc area ratio, normalised rim area to disc area ratio and retinal nerve fibre cross-sectional area for each patient at 10°-sectoral intervals. The values were ranked in descending order, and each ranked-segment curve of ordered values was fitted using the least squares method. Results: There was no difference in disc area between the groups. The group mean cup-disc area ratio was significantly lower in the normal group (0.204 ± 0.16) compared with the glaucoma group (0.533 ± 0.083) (p < 0.001). The visual field indices, mean deviation and corrected pattern S.D., were significantly greater (p < 0.001) in the glaucoma group (-9.09 dB ± 3.3 and 7.91 ± 3.4, respectively) compared with the normal group (-0.15 dB ± 0.9 and 0.95 dB ± 0.8, respectively). Univariate linear regression provided the best overall fit to the ranked segment data. The equation parameters of the regression line manually applied to the normalised rim area-disc area and the rim area-disc area ratio data, correctly classified 100% of normal subjects and glaucoma patients. In this study sample, the regression analysis of ranked segment parameters method was more effective than conventional ranked segment analysis, in which glaucoma patients were misclassified in approximately 50% of cases. Further investigation in larger samples will enable the calculation of confidence intervals for normality. These reference standards will then need to be investigated for an independent sample to fully validate the technique. Conclusions: Using a curve-fitting approach to fit ranked segment curves retains information relating to the topographic nature of neural loss. Such methodology appears to overcome some of the deficiencies of conventional ranked segment analysis, and subject to validation in larger scale studies, may potentially be of clinical utility for detecting and monitoring glaucomatous damage. © 2007 The College of Optometrists.

Hybrid approaches to estimating freeway travel times using point traffic detector data

The accurate and reliable estimation of travel time based on point detector data is needed to support Intelligent Transportation System (ITS) applications. It has been found that the quality of travel time estimation is a function of the method used in the estimation and varies for different traffic conditions. In this study, two hybrid on-line travel time estimation models, and their corresponding off-line methods, were developed to achieve better estimation performance under various traffic conditions, including recurrent congestion and incidents. The first model combines the Mid-Point method, which is a speed-based method, with a traffic flow-based method. The second model integrates two speed-based methods: the Mid-Point method and the Minimum Speed method. In both models, the switch between travel time estimation methods is based on the congestion level and queue status automatically identified by clustering analysis. During incident conditions with rapidly changing queue lengths, shock wave analysis-based refinements are applied for on-line estimation to capture the fast queue propagation and recovery. Travel time estimates obtained from existing speed-based methods, traffic flow-based methods, and the models developed were tested using both simulation and real-world data. The results indicate that all tested methods performed at an acceptable level during periods of low congestion. However, their performances vary with an increase in congestion. Comparisons with other estimation methods also show that the developed hybrid models perform well in all cases. Further comparisons between the on-line and off-line travel time estimation methods reveal that off-line methods perform significantly better only during fast-changing congested conditions, such as during incidents. The impacts of major influential factors on the performance of travel time estimation, including data preprocessing procedures, detector errors, detector spacing, frequency of travel time updates to traveler information devices, travel time link length, and posted travel time range, were investigated in this study. The results show that these factors have more significant impacts on the estimation accuracy and reliability under congested conditions than during uncongested conditions. For the incident conditions, the estimation quality improves with the use of a short rolling period for data smoothing, more accurate detector data, and frequent travel time updates.

Human Presence Detection in ambiente Home Manager: sperimentazione su piattaforma Raspberry

Home Manager, è una piattaforma sperimentale per la gestione di Smart Space e in particolare di una casa intelligente immersa in uno ambiente, avente l'ambizione di anticipare le necessità dell'utente. Questa tesi ha due obiettivi fondamentali: in primo luogo, implementare su piattaforma Raspberry la parte di Home Manager relativa allo scenario del riconoscimento delle persone negli ambienti della casa, mediante l'utilizzo del modulo telecamera; in secondo luogo, attraverso le informazioni ricavate precedentemente, implementare e simulare una gestione intelligente e automatica delle luci presenti all'interno della casa, sfruttando a tal fine un modulo relè.

Mobil-eye detector: lo studio del comportamento visivo del ciclista in ambito urbano

In questa tesi viene studiato il comportamento visivo del ciclista durante la guida in ambiente urbano diversificato, così da esaminare quali siano le diverse strategie visive adottate dal conducente della bicicletta, in rapporto al contesto in cui si trova. L’analisi del comportamento visivo è stata effettuata mediante l’esecuzione di test su campo, utilizzando il Mobile-Eye Detector, grazie al quale si sono potuti studiare i filmati relativi alle prove compiute da 12 utenti. In seguito ad una fase primaria di elaborazione generale dei dati e di scarto di quelli non ritenuti validi ai fini dello studio, sono state rilevate le fissazioni, le saccadi e i loro relativi tempi. In particolare, studiando i video, si è creato un database in cui vengono analizzate una ad una (fotogramma per fotogramma) le fissazioni e vengono individuati gli elementi ad esse relativi, quali: goal, target, pedoni, ciclisti, veicoli, ammaloramenti della pavimentazione, edifici, segnaletica verticale e orizzontale e intersezioni. Tali elementi sono quelli che attirano maggiormente l'attenzione del ciclista sia da un punto di vista pratico, ai fini della conduzione del mezzo, sia a fini di "svago". Inoltre sono state individuate delle aree di dispersione visiva, ovvero zone su cui è maggiormente ricaduto lo sguardo dei partecipanti all’esperimento: esse sono state studiate, analizzandone le dimensioni e mettendole in relazione col tipo di tragitto e con gli elementi fissati. In questo modo si è cercato di dare un'interpretazione del comportamento visivo del ciclista, sottoposto a tragitti con caratteristiche diverse. In seguito a tale analisi, è stata verificata la compatibilità tra i risultati ottenuti mediante i filmati visionati e le risposte date dai partecipanti nei questionari, proposti al termine della prova, finalizzati ad attestare la percezione soggettiva del ciclista.

Implementación de un detector de coral utilizando filtros Gabor Wavelets y máquinas de aprendizaje

Este trabajo se enfoca en la implementación de un detector de arrecife de coral de desempeño rápido que se utiliza para un vehículo autónomo submarino (Autonomous Underwater Vehicle, AUV, por sus siglas en inglés). Una detección rápida de la presencia de coral asegura la estabilización del AUV frente al arrecife en el menor tiempo posible, evitando colisiones con el coral. La detección de coral se hace en una imagen que captura la escena que percibe la cámara del AUV. Se realiza una clasificación píxel por píxel entre dos clases: arrecife de coral y el plano de fondo que no es coral. A cada píxel de la imagen se le asigna un vector característico, el mismo que se genera mediante el uso de filtros Gabor Wavelets. Éstos son implementados en C++ y la librería OpenCV. Los vectores característicos son clasificados a través de nueve algoritmos de máquinas de aprendizaje. El desempeño de cada algoritmo se compara mediante la precisión y el tiempo de ejecución. El algoritmo de Árboles de Decisión resultó ser el más rápido y preciso de entre todos los algoritmos. Se creó una base de datos de 621 imágenes de corales de Belice (110 imágenes de entrenamiento y 511 imágenes de prueba).

[the Use Of Fish On Buccal Smear To Investigate Mosaicism With A 45,x Cell Line: Study On Healthy Men And Patients With Disorders Of Sex Development].

To verify whether fluorescence in situ hybridization (FISH) of cells from the buccal epithelium could be employed to detect cryptomosaicism with a 45,X lineage in 46,XY patients. Samples of nineteen 46,XY healthy young men and five patients with disorders of sex development (DSD), four 45,X/46,XY and one 46,XY were used. FISH analysis with X and Y specific probes on interphase nuclei from blood lymphocytes and buccal epithelium were analyzed to investigate the proportion of nuclei containing only the signal of the X chromosome. The frequency of nuclei containing only the X signal in the two tissues of healthy men did not differ (p = 0.69). In all patients with DSD this frequency was significantly higher, and there was no difference between the two tissues (p = 0.38), either. Investigation of mosaicism with a 45,X cell line in patients with 46,XY DSD or sterility can be done by FISH directly using cells from the buccal epithelium.

Graded Meshes In Bio-thermal Problems With Transmission-line Modeling Method.

In this study, the transmission-line modeling (TLM) applied to bio-thermal problems was improved by incorporating several novel computational techniques, which include application of graded meshes which resulted in 9 times faster in computational time and uses only a fraction (16%) of the computational resources used by regular meshes in analyzing heat flow through heterogeneous media. Graded meshes, unlike regular meshes, allow heat sources to be modeled in all segments of the mesh. A new boundary condition that considers thermal properties and thus resulting in a more realistic modeling of complex problems is introduced. Also, a new way of calculating an error parameter is introduced. The calculated temperatures between nodes were compared against the results obtained from the literature and agreed within less than 1% difference. It is reasonable, therefore, to conclude that the improved TLM model described herein has great potential in heat transfer of biological systems.

Antitumor Activity Of Irradiated Riboflavin On Human Renal Carcinoma Cell Line 786-o.

Riboflavin (vitamin B2) is a precursor for coenzymes involved in energy production, biosynthesis, detoxification, and electron scavenging. Previously, we demonstrated that irradiated riboflavin (IR) has potential antitumoral effects against human leukemia cells (HL60), human prostate cancer cells (PC3), and mouse melanoma cells (B16F10) through a common mechanism that leads to apoptosis. Hence, we here investigated the effect of IR on 786-O cells, a known model cell line for clear cell renal cell carcinoma (CCRCC), which is characterized by high-risk metastasis and chemotherapy resistance. IR also induced cell death in 786-O cells by apoptosis, which was not prevented by antioxidant agents. IR treatment was characterized by downregulation of Fas ligand (TNF superfamily, member 6)/Fas (TNF receptor superfamily member 6) (FasL/Fas) and tumor necrosis factor receptor superfamily, member 1a (TNFR1)/TNFRSF1A-associated via death domain (TRADD)/TNF receptor-associated factor 2 (TRAF) signaling pathways (the extrinsic apoptosis pathway), while the intrinsic apoptotic pathway was upregulated, as observed by an elevated Bcl-2 associated x protein/B-cell CLL/lymphoma 2 (Bax/Bcl-2) ratio, reduced cellular inhibitor of apoptosis 1 (c-IAP1) expression, and increased expression of apoptosis-inducing factor (AIF). The observed cell death was caspase-dependent as proven by caspase 3 activation and poly(ADP-ribose) polymerase-1 (PARP) cleavage. IR-induced cell death was also associated with downregulation of v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homologue (avian)/protein serine/threonine kinase B/extracellular signal-regulated protein kinase 1/2 (Src/AKT/ERK1/2) pathway and activation of p38 MAP kinase (p38) and Jun-amino-terminal kinase (JNK). Interestingly, IR treatment leads to inhibition of matrix metalloproteinase-2 (MMP-2) activity and reduced expression of renal cancer aggressiveness markers caveolin-1, low molecular weight phosphotyrosine protein phosphatase (LMWPTP), and kinase insert domain receptor (a type III receptor tyrosine kinase) (VEGFR-2). Together, these results show the potential of IR for treating cancer.

Química forense: perspectivas sobre novos métodos analíticos aplicados à documentoscopia, balística e drogas de abuso

In this review recent methods developed and applied to solve criminal occurences related to documentoscopy, ballistic and drugs of abuse are discussed. In documentoscopy, aging of ink writings, the sequence of line crossings and counterfeiting of documents are aspects to be solved with reproducible, fast and non-destructive methods. In ballistic, the industries are currently producing ''lead-free'' or ''nontoxic'' handgun ammunitions, so new methods of gunshot residues characterization are being presented. For drugs analysis, easy ambient sonic-spray ionization mass spectrometry (EASI-MS) is shown to provide a relatively simple and selective screening tool to distinguish m-CPP and amphetamines (MDMA) tablets, cocaine and LSD.

Determinação rápida de fármacos básicos em plasma por cromatografia a gás com detector de nitrogênio e fósforo

A simple and fast method for determination of 40 basic drugs in human plasma employing gas-chromatography with nitrogen-phosphorus detection was developed and validated. Drugs were extracted from 800 µL of plasma with 250 µL of butyl acetate at basic pH. Aliquots of the organic extract were directly injected on a column with methylsilicone stationary phase. Total chromatographic run time was 25 min. All compounds were detected in concentrations ranging from therapeutic to toxic levels, with intermediate precision CV% below 11.2 and accuracy in the range of 92-114%.

Utilização da discriminação grafica de Fisher para indicação dos dermatoglifos como referencial de potencialidade de atletas de basquetebol

Universidade Estadual de Campinas. Faculdade de Educação Física