Benthic habitats of Buck Island Reef National Monument

NOAA’s Center for Coastal Monitoring and Assessment’s Biogeography Branch has mapped and characterized large portions of the coral reef ecosystems inside the U.S. coastal and territorial waters, including the U.S. Caribbean. The complementary protocols used in these efforts have enabled scientists and managers to quantitatively compare different marine ecosystems in tropical U.S. waters. The Biogeography Branch used these same general protocols to generate three seamless habitat maps of the Bank/Shelf (i.e., from 0 ≤50 meters) and the Bank/Shelf Escarpment (i.e., from 50 ≤1,000 meters and from 1,000 ≤ 1,830 meters) inside Buck Island Reef National Monument (BIRNM). While this mapping effort marks the fourth time that the shallow-water habitats of BIRNM have been mapped, it is the first time habitats deeper than 30 meters (m) have been characterized. Consequently, this habitat map provides information on the distribution of mesophotic and deep-water coral reef ecosystems and serves as a spatial baseline for monitoring change in the Monument. A benthic habitat map was developed for approximately 74.3 square kilometers or 98% of the BIRNM using a combination of semi-automated and manual classification methods. The remaining 2% was not mapped due to lack of imagery in the western part of the Monument at depths ranging from 1,000 to 1,400 meters. Habitats were interpreted from orthophotographs, LiDAR (Light Detection and Ranging) imagery and four different types of MBES (Multibeam Echosounder) imagery. Three minimum mapping units (MMUs) (100, 1,000 and 5,000 square meters) were used because of the wide range of depths present in the Monument. The majority of the area that was characterized was deeper than 30 m on the Bank/Shelf Escarpment. This escarpment area was dominated by uncolonized sand which transitioned to mud as depth increased. Bedrock was exposed in some areas of the escarpment, where steep slopes prevented sediment deposition. Mesophotic corals were seen in the underwater video, but were too sparsely distributed to be reliably mapped from the source imagery. Habitats on the Bank/Shelf were much more variable than those seen on the Bank/Shelf Escarpment. The majority of this shelf area was comprised of coral reef and hardbottom habitat dominated by various forms of turf, fleshy, coralline or filamentous algae. Even though algae was the dominant biological cover type, nearly a quarter (24.3%) of the Monument’s Bank/Shelf benthos hosted a cover of 10%-<50% live coral. In total, 198 unique combinations of habitat classes describing the geography, geology and biology of the sea-floor were identified from the three types of imagery listed above. No thematic accuracy assessment was conducted for areas deeper than about 50 meters, most of which was located in the Bank/Shelf Escarpment. The thematic accuracy of classes in waters shallower than approximately 50 meters ranged from 81.4% to 94.4%. These thematic accuracies are similar to those reported for other NOAA benthic habitat mapping efforts in St. John (>80%), the Main Eight Hawaiian Islands (>84.0%) and the Republic of Palau (>80.0%). These digital maps products can be used with confidence by scientists and resource managers for a multitude of different applications, including structuring monitoring programs, supporting management decisions, and establishing and managing marine conservation areas. The final deliverables for this project, including the benthic habitat maps, source imagery and in situ field data, are available to the public on a NOAA Biogeography Branch website (http://ccma.nos.noaa.gov/ecosystems/coralreef/stcroix.aspx) and through an interactive, web-based map application (http://ccma.nos.noaa.gov/explorer/biomapper/biomapper.html?id=BUIS). This report documents the process and methods used to create the shallow to deep-water benthic habitat maps for BIRNM. Chapter 1 provides a short introduction to BIRNM, including its history, marine life and ongoing research activities. Chapter 2 describes the benthic habitat classification scheme used to partition the different habitats into ecologically relevant groups. Chapter 3 explains the steps required to create a benthic habitat map using a combination of semi-automated and visual classification techniques. Chapter 4 details the steps used in the accuracy assessment and reports on the thematic accuracy of the final shallow-water map. Chapter 5 summarizes the type and abundance of each habitat class found inside BIRNM, how these habitats compare to past habitat maps and outlines how these new habitat maps may be used to inform future management activities.

Field applications of the second-generation Environmental Sample Processor (ESP) for remote detection of harmful algae: 2006-2007

We assess the application of the second-generation Environmental Sample Processor (ESP) for the detection of harmful algal bloom (HAB) species in field and laboratory settings using two molecular probe techniques: a sandwich hybridization assay (SHA) and fluorescent in situ hybridization (FISH). During spring 2006, the first time this new instrument was deployed, the ESP successfully automated application of DNA probe arrays for various HAB species and other planktonic taxa, but non-specific background binding on the SHA probe array support made results interpretation problematic. Following 2006, the DNA array support membrane that we were using was replaced with a different membrane, and the SHA chemistry was adjusted. The sensitivity and dynamic range of these modifications were assessed using 96-well plate and ESP array SHA formats for several HAB species found commonly in Monterey Bay over a range of concentrations; responses were significantly correlated (p < 0.01). Modified arrays were deployed in 2007. Compared to 2006, probe arrays showed improved signal:noise, and remote detection of various HAB species was demonstrated. We confirmed that the ESP and affiliated assays can detect HAB populations at levels below those posing human health concerns, and results can be related to prevailing environmental conditions in near real-time.

Algae and water pollution in Mahi Estuary

Distribution of phytoplankton, chlorophyll A and phaeophytin was studied at different locations in the Mahi Estuary, Gujarat, India during 1982. The water quality at the discharge point was poor while the region away from it was relatively unpolluted. The results indicated a wide range of variation in phytoplankton population (7.68-5010, 96 x 10 super(4) cells/l, chl. a 2.22-58.22 mg/m super(3) and phaeophytin [0.20-10.21 mg/m super(3)]. The ratio of chl. a/phaeophytin were remarkably low at highly polluted stations. Higher abundance of the genera Oscillatoria and Nitzschia were recorded at polluted stations. The diversity of species at the unpolluted station was higher (1.56) as compared to the polluted station (1.07).

Sterol composition of marine algae from Karachi coast of Arabian Sea

During the course of chemical investigation of marine algae collected from Karachi coast of Arabian Sea, five sterols named as sarangosterol(1), 23-methyl cholesta-5, 25-dien-3ß-ol(2) from Endarachne binghamiae (brown alga), sargasterol(3) from Dictyota indica (brown alga), cholesterol(4) from Laurencia obtusa (red alga) and clerosterol(5) from Codium iyengarii (green alga) have been isolated. Their structures were elucidated with the help of spectroscopic means.

Acyclic diterpene alcohols isolated from four algae of Bryopsidophyceae and their toxicity

Three new acylic diterpenoids belonging to the class of phytol series have been isolated. They were obtained from the ethyl acetate soluble fractions of four siphonaceous green seaweeds, Bryopsis pennata Lamour., Caulerpa taxifolia (Vahl) C. Ag., Codium decorticatum (Woodw.) Howe and Valoniopsis pachynema (Mart.) Børg., collected from Karachi coast of Pakistan. Structures of these compounds were elucidated with the help of spectroscopic methods and confirmed by comparison with the known compounds. Even the known compounds are being reported for the first time from a green algal source. All the compounds were found to display a strong toxicity at all the three concentrations tested in the brine shrimp bioassay.

Contribution to the study of the marine algae of Ceylon

Description of Ceylon marine algae are scattered through a range of scientific publications which are not readily available to students and research workers in Ceylon. There are few detailed studies in Algal taxonomy and the general distribution of algae in Ceylon has not been studied thoroughly. Some work has been done by foreign phycologist each of whom was in the island for a few months only and experienced difficulty collecting specimens from different parts of Ceylon.

Some marine algae from Ceylon - 1

Since his last publication "Contribution to the Study of the Marine Algae of Ceylon"(1961), the author has made several collections which contain interesting material which he has not hitherto described. This and any other material which he come across in future surveys will be described in a series of papers of which this is the first. The present paper includes the description of fourteen species of which three are new to science. These are deposited in the Herbarium of the Royal Botanical Gardens, Peradeniya, Ceylon.

Preliminary trials on the toxicity of "Algistat" to fish and algae

Preliminary toxicity trials conducted with "Algistat" (an algicide) indicated that a dose of 0,66ppm of the compound was highly toxic to fish and 0.8ppm was the general lethal level for Spirogyra sp. The blue green algae, Oscillatoria sp., Mycrocystis sp. and Anabaena sp. were adversely affected by dosages higher than 0.5 ppm Euglena sp. was not affected even at 1.0 ppm.

Taxonomic study of brown algae commonly growing on the coast of Karachi, Pakistan

Fourteen commonly occurring species of marine benthic algae, i.e., Colpomenia sinuosa (Mertens ex Roth) Derbes et Solier, Dictyopteris australis (Sonder) Askenasy, Dictyota dichotoma (Hudson) Lamouroux D. dumosa Børgesen, D. hauckiana Nizamuddin, D. indica Sonder, D. maxima Zanardini, Hincksia mitchelliae (Harvey) Silva, Jolyna laminarioides Guimardes in Guimarâes et al., Padina tetrastromatica Hauck, Sargassum tenerrinum J. Agardh, Spatoglossum variabille Figari et De Notaris, Stoechospermum marginatum (C. Agardh) Kültzing and Stokeyia indica Thivy et Doshi, belonging to all three classes of Phaeophyta, were collected from coastal areas near Karachi, Pakistan and their taxonomy determined. Although all are taxonomically known species, Dictyota dichotoma, D. dumosa, D. hauckiana, D. indica, D. maxima, Jolyna laminarioides, Padina tetrastromatica, Sargassum tenerritnum and Stokeyia indica are described for the first time from the coast of Pakistan.

Some marine algae from Ceylon - 2. Laurencia lamouroux

The herbarium material belonging to the genus Laurencia kept at the Royal Botanical Gardens, Peradeniya together with my collections of material belonging to this genus from various parts of Ceylon have been examined. Most of the material belonging to the genus Laurencia had been incorrectly identified and their true identity has been determined. A key to the Ceylon species of Laurencia is given.

Systematic study of the blue green algae of Ceylon kept at the herbarium of the Royal Botanical Gardens, Peradeniya

The collection of blue green algae kept at the herbarium of the Royal Botanical Gardens, Peradeniya, was collected by Ferguson more than 70 years ago. Since then many changes have taken place in the taxonomy of the blue green algae. West (1902), Lemmermann (1907), Wine (1915), Crow (1923), Bharadwaja (1934) and Holsinger (1935) had described some of the blue green algae of Ceylon. While examining the collections of blue green algae kept at the herbarium, the authors found that most of the identifications were incorrect and required revision. In the present paper 20 blue green algae are described. The classification and key to the species are based on the characters given by Desikachary (1959). Blue green algae are important to fisheries since Chanos larvae feed on them.

Antibacterial properties of some marine algae of Sri Lanka

The authors report on the antibacterial activity of 11 algae, green, brown and red. Prepared extracts were tested, and varying degrees of activity were exhibited. Most activity was shown by Ulva fasciata, Sargassum cervicone and Halimeda macroloba.

A taxonomic study of some red algae commonly growing on the coast of Karachi

Eighteen commonly occurring species of marine benthic red algae, i.e., Asparagopsis taxiformis (Delile) Trevisan, Bangia atropurpurea (Roth) C. Agardh, Centroceras clavulatum (C. Agardh) Montagne, Calliblepharis fimbriata (Greville) Kiitzing, Coelarthrum muelleri (Sonder) B¢rgesen, Cottoniella filamentosa (Howe) B¢rgesen, Gracilariafoliifera (Forsskai) B¢rgesen, Halymenia porphyraeformis (B¢rgesen) Parkinson, Hypnea muscifor:mis (Wulfen) Lamouroux, Hypnea valentiae (Turner) Montagne, Laurencia obtusa (Hudson) Lamouroux, Me!anothamnus somalensis Bomet et Falkenberg, Porphyra vietnamensis Tanaka et Pham-hoang H6, Sarconema filiforme (Sonder) Kylin, Sebdenia flabellata (J. Agardh) Parkinson, Scinaia fascicularis (B¢rgesen) Huisman, Scinaia hatei B¢rgesen, and Solieria robusta (Greville) Kylin were collected from coastal areas near Karachi (Pakistan) and taxonomically investigated. All the investigated seaweeds are taxonomically known species. During this study, Melanothamnus somalensis is reported for the first time from northern Arabian Sea and Asparagopsis taxiformis, Bangia atropurpurea, Cottoniella filamentosa, Gracilaria foliifera, Halymenia porphyraeformis, Melanothamnus somalensis, Sarconema filiforme, Sebdenia flabellata, Scinaia fascicularis, and Solieria robusta are taxonomically described for the first time from the coast of Pakistan.

Growth and reproductive performance of locally isolated brackish water rotifer (Brachionus plicatilis) feeding different micro algae

Population growth and reproductive capacity of brackishwater rotifer, Brachionus plicatilis, were evaluated, for a period of 8 days in a temperature controlled ( =25°C) microalgallaborarory, under three different algal feeding regimens. The algal species that were tested are: (i) Chlorella sp. (T1), Tetraselmis chui (T2), Nannochloropsis oculata (T 3). The feeding density of each algal species was maintained similar as of 4.5xW6 ceHs mi. The rotifer fed on T. chui showed the highest (p<0.05) population growth (131.5 ind./ml), compared to that fed on Chlorella sp (45.67 ind./ml) and N oculata (43.44 ind./ml). The abundance of egg bearing rotifers was also higher (35.77%) with T. chuithan with Chlorella sp (27.76%) and N oculara (24.60%). The results of the present study indicate that T. chui could be the most suitable algal food for the stock culture of locally isolated rotifer B. plicatilis.