Evaluación de la primera alimentación en larvas de capaz pimelodus grosskopfii bajo condiciones de laboratorio

Evaluate the performance and survival of capaz larvae Pimelodus grosskopfii after supplying live food (Cladocera, Copepod and Artemia Salina). Materials and methods. Capaz larva were placed in plastic containers with an useful volume of 3 L , and a larvae density of 10 L-1, they were fed four times a day during 15 days with newly hatched Artemia nauplii, Cladocerans of the genders Moina and Ceriodaphnia and Copepods calanoides. Capaz larva were weighed and measured at the beginning and the end of the experiment in order to evaluate their weight gain (WG), length gain (LG), specific growth rate (SGR), relative growth factor (RGF), and survival rate(S). Results. The treatment that showed the best results in WG, LG and S was the lot fed with Artemia nauplii (3.8±0.2 mg, 8±0.7 mm and 48.3% respectively), followed by sample supplied with Cladocera and Copepods. Conclusions. The Artemia nauplii treatment revealed the best results on the productive variables evaluated in P. grosskopfii larvae at the beginning of their exogenous feeding.

Desempeño de tanques decantadores de sólidos en un sistema de recirculación para producción de tilapia

To compare the removal efficiency of solids, turbidity and apparent color between a conventional and a column settling tanks in a recirculating aquaculture system (RAS) for tilapia farming. Materials and methods. Tilapia with a stocking density between 30 and 33 kg/m3 were cultured in a RAS consisting of a water level control box, PVC piping system, three plastic tanks for culture, conventional horizontal flow settling tank (Con.ST), column vertical flow settling tank (Col.ST), three phase fluidized bed reactor, oxygen transfer reactor, air compressor, air blower, centrifugal pump. The Con.ST operated at a volume of 1.4 m3 and hydraulic retention time (HRT) of 2.94 h; and was drained weekly for washing and sludge collection, representing a 55%discharge of system water volume. The Col.ST operated with a volume of 0.30 m3 and HRT of 0.553 h. Three daily partial draining operations were executed, representing a discharge of 50% of the system volume. Results. The mean solids removal efficiencies were: 34.01 and 44.44%for total solids; 64.45 and 71.71% for suspended solids; 21.10 and 45.65% volatile solids; 65.51% and 62.79% for turbidity; and 56.37 and 50.91% for apparent color, respectively for Con.ST and Col.ST. Conclusions. The two settling devices are useful on removal of the studied parameters and presented similar performance on turbidity and apparent color removal; however, the Col.ST was more efficient than Con.ST for solids removal, requires less space, less volume and requires less discharge water volume, displaying feasibility for its use on RAS.

Hacia una nueva estrategia del desarrollo de los recursos mineros de América Latina y el Caribe

Incluye Bibliografía

Interação genótipo-ambiente para a produção de leite em bubalinos no Brasil e na Colômbia

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Lectinhistochemical staining of granuloma induced by bacillus Calmette- Guerin in Piaractus mesopotamicus

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Influence of glyceryl guaiacolate ether on anesthetics in tilapia compared to benzocaine and eugenol

Objective. The study aimed to investigate the effectiveness of glyceryl guaiacolate ether (GGE) and compare the times of induction, recovery, hematological changes, total protein and glycaemia among anesthetics in Nile tilapia, Oreochromis niloticus. Materials and methods. A total of 60 tilapia distributed in 3 aquariums (N=20) were used, which formed the group benzocaine (100 mg/L), eugenol (50 mg/L) and guaiacol glyceryl ether (9.000 mg/L). After the induction of anesthesia fish blood samples were collected to determine the complete hemogram and glycemia. Then the animals were placed in aquariums with running water for assessing the anesthesia recovery. Results. It was verified that GGE showed longer induction and recovery times as well a significant increase (p<0.05) of glycemia, when compared with the other groups (p<0.05). The concentration of total protein did not differ between groups (p>0.05). An increase in the number of monocytes in the group treated with benzocaine (p<0.05) was observed in the analysis of the hematological parameters with no difference between groups for other variables. Conclusions. Eugenol and benzocaine allow rapid induction and recovery in Nile tilapia, without evidence of stress during handling and GGE showed high induction and recovery times, being inadequate for anesthetic use in Nile tilapia.

Accumulated productivity and its genetic association with reproductive traits in females Brahman

Objective. Assessment of genetic parameters for accumulative productivity trait (ACP) and genetic correlations with age at first calving (AFC), between calving interval of first and second parity (BCI1) and longevity (LONG). Materials and methods. 8584 Brahman female records were used with an animal model in multi-trait analysis with restricted maximum likelihood method, implemented using the WOMBAT software. The models considered the fixed effects of contemporary group, parity and weaning weight of first calf covariate, the only random effect was the genetic additive direct. Weaning weight (P240) was included to reduce the effect of selection on the estimation of variance components. Results. The heritability estimates were 0.3 +/- 0.04, 0.11 +/- 0.03, 0.07 +/- 0.03 and 0.24 +/- 0.04 for AFC, BCI1, LONG and ACP respectively. Correlations between ACP and the other features were moderate to high and favorable. Conclusions. ACP can be included in breeding programs for Brahman, and used as selection criteria for its moderate heritability and genetic correlation with reproductive traits.

The Mutagenic Potential Caused by the Emissions from Combustion of Crude Glycerin and Diesel Fuel

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Maternal-calf relationships and their influence on calves up to 120 days

Objectives. To research parental behavior and measure the weight of Nelore and Guzerat calves up to 120 days of age, collecting data from birth up to the first lactation. Materials and methods. Seven different corporal positions were considered to obtain specific data for each of the following categories: time to stand (TP), time to intend to feed after standing (TPTM), weight at birth (PN), weight at 120 days (P120), and time in contact with the calf (TCC). Additionally, independent variables were also evaluated, organized by class: weight at birth (CPN), number of calvings (CNP), order of calving in relation with the herd (CLPR), time of calving (CHP), and finally, hindering to feed (CTAT). Results. There was a significant result in favor of the Nelore race over the Guzerat race. The statistical averages for Nelore and Guzerat were: Time to stand averaged 71 and 153 minutes; time to intend to feed after standing, 39 minutes for Nelore and of 114 minutes for Guzerat. For variable weight at 120 days, Nelore reached an average of 122 kg, and Guzerat 109 kg. For the time in contact with the calf variable there was no significant variance between races. Conclusions. Results showed the superiority of the Nelore race, considering it better adapted to local environmental conditions.

Random regression models for milk, fat and protein in Colombian Buffaloes

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Pulmonary adenocarcinoma in cattle

The Macroscopic, histological and immunohistochemical aspects of lung acinar adenocarcinoma and the presence of nodules in the abdominal cavity of an adult female bovine are reported. In the necropsy analysis samples were collected from the: lung, heart, spleen, liver, pancreas, kidney, uterus, intestine, brain, and from nodules found in the lung and abdominal cavity, which were routinely processed to be stained by hematoxylin-eosin and for an immunohistochemistry exam with the antibodies: cytokeratin (dilution 1: 200 mu L) and vimentin (dilution 1: 1000 mu L). The histopathological examination revealed neoplastic epithelial cells with acini formation. The immunohistochemical examination of the tumor cells showed positive marking for cytokeratin and the absence of marking for vimentin. According to anatomical, morphological, and histopathological findings, as well as the result of the immunohistochemical examination, the tumor was characterized as lung acinar adenocarcinoma.

Kinetic and Thermodynamic Studies on the Adsorption of Reactive Red 239 by Carra Sawdust Treated with Formaldehyde

In this study, carra sawdust pre-treated with formaldehyde was used to adsorb reactive red 239 (RR239). The effects of several experimental conditions, including the concentration of dye, sorbent dosage, temperature, ionic strength, stirring speed and solution pH, on the kinetics of the adsorption process have been studied, and the experimental data were fitted to pseudo-second-order model. A study of the intra-particle diffusion model indicates that the mechanism of dye adsorption using carra sawdust is rather complex and is most likely a combination of external mass transfer and intra-particle diffusion. The experimental data obtained at equilibrium were analyzed using the Langmuir and Freundlich isotherm models, and the results indicated that at this concentration range, both models can be applied for obtaining the equilibrium parameters. The maximum dye uptake obtained at 298 K was found to be 15.1 mg g(-1). In contrast to the usual systems, the reactive dye studied in the present work is strongly attached to the sawdust even after several washes with water, allowing it to be discarded as a solid waste.

Conformational polymorphism of the antidiabetic drug chlorpropamide

In this paper, the main features of Raman spectroscopy, one of the first choice methods in the study of polymorphism in pharmaceuticals, are presented taking chlorpropamide as a case of study. The antidiabetic drug chlorpropamide (1-[4-chlorobenzenesulphonyl]-3-propyl urea), which belongs to the sulfonylurea class, is known to exhibit, at least, six polymorphic phases. These forms are characterized not only by variations in their molecular packing but also in their molecular conformation. In this study, the polymorphism of chlorpropamide is discussed on the basis of Raman scattering measurements and quantum mechanical calculations. The main spectroscopic features that fingerprint the crystalline forms are correlated with the corresponding crystalline structures. Using a theoretical approach on the energy dependence of the conformers, simulated molecular torsion angles are plotted versus the formation energy, which provides a satisfactory agreement between the torsion angles at the energy minima and the experimental values observed in the different solid forms of chlorpropamide. Copyright (C) 2011 John Wiley & Sons, Ltd.

Galaxy triplets in Sloan Digital Sky Survey Data Release 7-I. Catalogue

We present a new catalogue of galaxy triplets derived from the Sloan Digital Sky Survey (SDSS) Data Release 7. The identification of systems was performed considering galaxies brighter than Mr=-20.5 and imposing constraints over the projected distances, radial velocity differences of neighbouring galaxies and isolation. To improve the identification of triplets, we employed a data pixelization scheme, which allows us to handle large amounts of data as in the SDSS photometric survey. Using spectroscopic and photometric data in the redshift range 0.01 =z= 0.40, we obtain 5901 triplet candidates. We have used a mock catalogue to analyse the completeness and contamination of our methods. The results show a high level of completeness ( 80 per cent) and low contamination ( 5 per cent). By using photometric and spectroscopic data, we have also addressed the effects of fibre collisions in the spectroscopic sample. We have defined an isolation criterion considering the distance of the triplet brightest galaxy to the closest neighbour cluster, to describe a global environment, as well as the galaxies within a fixed aperture, around the triplet brightest galaxy, to measure the local environment. The final catalogue comprises 1092 isolated triplets of galaxies in the redshift range 0.01 =z= 0.40. Our results show that photometric redshifts provide very useful information, allowing us to complete the sample of nearby systems whose detection is affected by fibre collisions, as well as extending the detection of triplets to large distances, where spectroscopic redshifts are not available.

Improving the performance of a glucose biosensor using an ionic liquid for enzyme immobilization. On the chemical interaction between the biomolecule, the ionic liquid and the cross-linking agent

The effect of the room temperature ionic liquid (1-butyl-2,3-dimethylimidazolium tetrafluoroborate ([BMMI][BF4])) on the immobilization of glucose oxidase (GOx) was studied. The electrochemical performance of biosensors prepared following different protocols indicated a beneficial effect of the ionic liquid on the analytical parameters. The chemical interaction between GOx, [BMMI][BF4] and glutaraldehyde was investigated using UV-visible spectroscopy (UV-vis) and circular dichroism (CD). Structural changes of the biomolecule were observed to depend on the method used for the immobilization. (C) 2011 Elsevier Ltd. All rights reserved.