Inorganic polyphosphate and the induction of rpoS expression

Inorganic polyphosphate [poly(P)] levels in Escherichia coli were reduced to barely detectable concentrations by expression of the plasmid-borne gene for a potent yeast exopolyphosphatase [poly(P)ase]. As a consequence, resistance to H2O2 was greatly diminished, particularly in katG (catalase HPI) mutants, implying a major role for the other catalase, the stationary-phase KatE (HPII), which is rpoS dependent. Resistance was restored to wild-type levels by complementation with plasmids expressing ppk, the gene for PPK [the polyphosphate kinase that generates poly(P)]. Induction of expression of both katE and rpoS (the stationary-phase σ factor) was prevented in cells in which the poly(P)ase was overproduced. Inasmuch as this inhibition by poly(P)ase did not affect the levels of the stringent-response guanosine nucleotides (pppGpp and ppGpp) and in view of the capacity of additional rpoS expression to suppress the poly(P)ase inhibition of katE expression, a role is proposed for poly(P) in inducing the expression of rpoS.

What to do till the doctor comes? Frank Capra e a Grande Depressão

Nenhum outro realizador a trabalhar em Hollywood durante os anos 30 do século XX alcançou maior notoriedade do que Frank Capra. A sua biografia, tão parecida com as histórias de rags to riches, parece coexistir com as narrativas ilustradas em alguns dos seus filmes mais perenes. No entanto, apesar de todo este sucesso, a carreira de Frank Capra seria relativamente curta e, sobretudo, quase exclusivamente associada à década da Grande Depressão. A elaboração deste trabalho tem por objetivo articular a história pessoal de Frank Capra com as histórias da Grande Depressão, utilizando os filmes que dirigiu durante essa década e, neste processo, tornar aparentes alguns dos motivos para o sucesso do realizador durante os anos 30.

The Liber studiorum of J.M.W. Turner, R.A., in the Victoria and Albert Museum.

"This catalogue has been compiled by Mr. E.F. Strange".

L'Espagnole /

"Tirage: cinq cents exemplaires ... 350 vélin du Marais (nos 151 à 500). no. 208."

Some account of the manor of Apuldrefield in the parish of Cudham, Kent,

"From the 'Topographer and genealogist', vol. III."

The U. S. Grinnell Expedition in search of Sir John Franklin : a personal narrative /

Mode of access: Internet.

The dispensation, and other plays,

The dispensation.--The star of Bethlehem.--"Through Christmas bells."--The awakening of Barbizon.

The letters of the Rev. John Wesley ...

First edition.

La Nuova Italia: dizionario amministrativo, statistico, industriale, commerciale ...

Mode of access: Internet.