Studies on threshold current densities for convulsion of fish using a square wave stimulator

The threshold current densities and voltage tensions (body voltages) between the head and tail for bringing about distinct reactions in Salmo irideus, Cyprinus Carpio, Tinea tinca, Gasterosteus aculeatus and Salmo fario were studied. In C. carpio and T. tinca, absolute current densities required decreased with increase in length of fish. Threshold current densities for different reactions of fish increased with rise in water temperature and conductivity of surrounding medium except in case of T. tinca where low current densities were sufficient in higher conductivity of water. Impulse D.C. was superior to continuous D.C. Better effect was noticed in fishes in lower current densities when their bodies were parallel to the lines of current conduction.

Genetic diversity in crucian carp (Carassius auratus)

A survey of restriction fragment polymorphism in mitochondrial DNA of three subspecies of Carassius auratus throughout four provinces in China was undertaken using 17 restriction enzymes. Two carp, Cyprinus carpio rubbrofuscus and Cyprinus carpio carpio, were included as the outgroup. A total of 16 haplotypes was observed: 5 in tetraploids of C. auratus auratus; 8 in hexaploids of C. auratus auratus; and 2 in C. auratus gibelio and C. auratus cuvieri, respectively. The tetraploids and hexaploids share three common haplotypes as I, V, and VI. C. a. Cuvieri may have diverged first among the three subspecies. Interestingly, C. a. auratus and C. a. cuvieri did not form monophyletic clades, which indicated that the classification of carassius auratus required further studies. The current hypothesis, that hexaploids originated from tetraploids by a polyploidy event, is less favorable, based on the distribution of haplotypes and the lower diversity in tetraploids than in hexaploids. Our data also indicate that divergence of hexaploids and tetraploids might be recent and mtDNA polymorphism existed before the divergence. Meanwhile, genetic isolation exists between the hexaploids and the tetraploids.

Nucleotide degradation and production of hypoxanthine in some Indian marine and freshwater fishes

Changes in nucleotides and production of hypoxanthine in rohu (Labeo rohita), mrigal (Cihhrina mrigala) and common carp (Cyprinus carpio) during storage at 2-4°C were examined. Differences were observed between common carp and others. Production of hypoxanthine in pomfret (Stromateus argenteus), cat fish (Arius macronotacanthus), shark (Scoliodon spp.), seer fish (Scomberomorus guttatus), ray fish (Dasyatis imbricata) and prawns (Parapenaeopsis stylifera) was examined during storage at 2-4°C and -28°C. At 2-4°C hypoxanthine increased regularly but at -28°C there was no increase during storage over 28 weeks.

The complete mitochondrial genomes of two species from Sinocyclocheilus (Cypriniformes: Cyprinidae) and a phylogenetic analysis within Cyprininae

We determined the complete mitochondrial DNA sequences for two species of surface- and cave-dwelling-cyprinid fishes, Sinocyclocheilus grahami and S. altishoulderus. Sequence comparison of 13 protein-coding genes shows that the mutation pattern of each single gene is quite similar to those of other vertebrate animal species. Analysis of the ratios of Ka/Ks at these loci between Sinocyclocheilus and two other cyprinid species (Cyprinus carpio and Procypris rabaudi) show that Ka/Ks ratios are differed, consistent with purifying selection and variation in functional constraint among genes. Bayesian analysis and maximum likelihood analysis of the concatenated mitochondrial protein sequences for 14 cyprinid taxa support the monophyly of the family Cyprininae, and further confirm the monophyly of the genus Sinocyclocheilus. The two Sinocyclocheilus species fall within the Cyprinion-Onychostoma lineage, including Cyprinus, Carassius, and Procypris, rather than among the Barbinae, as previously suggested on morphological grounds.

Integrated cage-cum-pond culture systems with high valued climbing perch (Anabas testudineus) in cages and low-valued carps in open ponds

An on-farm trial was conducted over 150 days to determine appropriate stocking ratio, growth and production of climbing perch (Anabas testudineus) in cages and carps in open water of ponds in eighteen farmers' ponds from Haluaghat Upazila at Mymensingh district of Bangladesh. One or two 1 m super(3) cage was suspended in each of 12 earthen ponds and other 6 ponds served as control without cages. Climbing perch of 2-3 g in size were stocked in cages while fingerlings of silver carp (Hypophthalmicthys molitrix), catla (Catla catla), rohu (Labeo rohita), mirgal (Cirhinus cirrhosus), rajputi (Puntius sarana) and common carp (Cyprinus carpio) were stocked at 1 fish/m super(2) with a species ratio of 5:4:4:4:2:1 in open water of all ponds to give cage to open-pond fish ratios of 1:1 (T sub(1:1)) and 2:1 (T sub(2:1)) and 0:1 (T sub(0:1)) as three treatments with six replicates each. Survival of climbing perch was higher in T sub(1:1) (61.67%) than that of T sub(2:1) (29.5%) and was significantly different (p>0.05) between the treatments. Stocking of small size climbing perch fry increased the mortality rate in cages. The net yields of Thai koi were 0.13±0.01 (t/ha) and 0.10±0.01 (t/ha) in treatments T sub(1:1) and T sub(2:1), respectively and both were significantly different (p>0.05). Survival of-open-pond carps was high, ranging from 50 to 91.67% with significantly lower in T sub(0:1) than that of T sub(1:1) and T sub(2:1) treatment. Net and gross yield of each carp species were significantly higher in the T sub(1:1) and T sub(2:1) treatment than that in T sub(0:1) treatment. Net revenues were positive but low in all treatments. Therefore, bigger size climbing perch with lower stocking ratio (T sub(1:1)) is suitable for integrated cage-pond culture of climbing perch and carps. However, more on-farm trials in different ecosystem with scientific interventions are necessary to develop the technology for further dissemination among the rural farmers.

Distribution, prevalence and intensity of protozoan and monogenean parasites of carp fingerlings in selected nursery ponds

An experiment was carried out on the distribution, prevalence and intensity of infestation of protozoan and monogenean parasites of carp fingerlings in two selected areas of Bangladesh. Six hundred and forty fingerlings of seven species viz. Catla calla, Hypophthalmicthys molitrix, Labeo rohita, Ctenophalyngodon idella, Cyprinus carpio, Barbodes gonionotus and Cirrhinus cirrhosus from different nursery ponds of Shambhuganj, Mymensingh, and Santaher, Bogra were examined during this investigation. This study revealed that carp fingerlings carried a large number of protozoan and monogenean parasites. Different protozoan parasites viz Trichodina domerguei, Trichodina reticulata, Myxobolus koi, Chilodonella cyprini and monogenean parasites such as Dactylogyrus extensus, Dactylogyrus catlarius and Dactylogyroides tripathi were identified in the experimental fishes throughout the study period. It has been observed that distribution, prevalence and intensity of parasite in carp fingerlings are species and zone specific. In case of C. cirrhosus, the highest prevalence of protozoan parasite viz Trichodina domerguei were 93.75% and 75.92% in Santhahar and Shambhuganj area respectively. Irrespective of host, the most prevalent ectoparasite was Dactylogyrus extensus, followed by Myxobolus koi, Chilodonella cyprini and Dactylogyroides tripathi. Relatively higher mortality of carp fingerlings was found at younger stage. The highest prevalent parasitic species was identified as Trichodina domerguei followed by Trichodina reticulata and Dactylogyrus catlarius were found 93.75% in Cirrhinus cirrhosus, 68.75% in Cyprinus carpio and 39.44% in Hypophthalmicthys molitrix respectively.

Studies on the growth and production of six major and exotic carps in Nasti baor, Jhenaidah, Bangladesh

An investigation on growth, production and fishery of three Indian major carps: rohu, Labeo rohita, catla, Catla catla and mrigal, Cirrhinus mrigala and three exotic carps: silver carp, Hypophthalmichthys molitrix, grass carp, Ctenopharyngodon idella, and common carp, Cyprinus carpio was carried out in Nasti baor during February to April months. In catch per unit effort (CPUE) study the highest catch/day/person (3.13 kg) and catch/day/gear (40.65 kg) was recorded in the month of March for kochal fishing. In komar fishing catch/day/person (15.08 kg) and catch/day/gear (1206 kg) was also found higher in March. Komar fishing was done only in March and April and its CPUE was greater in both the months than that of kochal. The average recovery rate (combination of all six species) was 37.80 considering the stocking from July month of the previous year. The recovery rate of common carp (54.1) was the highest and lowest (13.90) in case of silver carp. When the recovery was calculated on the basis of one year data and stocking, it was 55.6%. Analysis of production model revealed that the present production (54,806 kg/year) is less than both theoretical production (model I- 85,285 kg/year and model II -75,952 kg/year) estimated. Therefore, it may be concluded that the fish production from Nasti baor could still be increased from the present level of production.

What you should know about carp: its origin, varieties, physical appearance, feeding habits

The article discusses the different varieties of carp, their origin, physical appearance and feeding habits. The species discussed are grass carp (Ctenopharyngodon idella), silver carp (Hypophthalmichthys molitrix), bighead carp (Aristichthys nobilis), catla (Catla catla), rohu (Labeo rohita), mrigal (Cirrhinus mrigala), and common carp (Cyprinus carpio).

Gross clinical signs and haematological changes associated with artifical infection of Edwardsiella tarda in Koi Carp

The occurrence of diseases is a significant setback for successful aquafarming. One of the common fish bacterial disease syndromes, Edwardsiellosis is caused by Edwardsiella tarda, a gram-negative, rod shaped bacterium associated with several diseases of marine and fresh water fish. In this study, an attempt was made to observe and analyze the onset of clinical symptoms and certain haematological parameters in Koi Carp, Cyprinus carpio L., following artificial infection with Edwardsiella tarda. The disease progress was observed and the clinical symptoms were monitored over a period of 15 days following infection. Fish were sampled at three day intervals to analyse the haematological parameters: total erythrocyte counts (RBC), total leucocyte counts (WBC), haemoglobin content and differential leucocyte count. Clinical symptoms observed included: erratic swimming behaviour, loss of appetite, haemorrhages, dropsy and exophthalmia. There was a significant decrease in the total RBC and haemoglobin levels by the 3rd and 6th day post infection, and an increase thereafter. WBC counts were higher in all infected groups in comparison to the control group. A significant increase in the number of neutrophils was found in the infected group up to the 9th day and a decrease thereafter. The lymphocyte number was significantly less up to the 12th day while the monocyte counts were significantly higher up to the 12th day post infection. The results showed that the bacterium, E. tarda, is pathogenic to Koi Carp. The hematological changes and clinical signs in infected fish reported in this paper will be helpful in the identification and the control of this infection.

云南两种珍稀鱼类的形态度量学和分子系统学研究

鱇鱼良白鱼（Anabarilius grahami）、大头鲤（Cyprinus pellegrini）均隶 属于鲤形目（Cypriniformes）鲤科（Cyprinidae），是中国特有鱼类之一，仅在 云南省分布，是抚仙湖和星云湖各自的主要经济鱼类。 本研究首先基于线粒体控制区全序列来研究我国抚仙湖鱇鱼良白鱼自然种群 及人工驯养种群的遗传多样性，发现鱇鱼良白鱼人工驯养种群遗传多样性较高，种 群复壮程度良好；自然种群遗传多样性匮乏，可能种群经历过“建群者效应”和 “瓶颈效应”；并且各自然地理居群具有广泛的基因交流，不存在遗传分化，建 议应被当成一个管理单元进行整体保护；同时，对照其他已报道的鱼类控制区结 构，对鱇鱼良白鱼控制区结构进行了分析，识别了其终止序列区、中央保守区和保 守序列区，找到了终止相关的序列TAS 以及保守序列（CSB-F、CSB-D、CSB-1、 CSB-2、CSB-3）。 其次，采用主成分分析方法对大头鲤及与其相关的三个鲤鱼类群——杞麓鲤 （Cyprinus chilia）、华南鲤（Cyprinus rubrofuscus）及柏元鲤（Cyprinus carpio var. yuankiang×Cyprinus pellegrini）进行了可量性状分析，了解了 不同类群的外部差异；依据主成分分析结果，运用逐步判别方法从37 个可量性 状中筛选出能有效鉴定大头鲤的10 项形态指标：头长、吻长、吻宽、眼间距、 尾柄高、头背部末端到腹鳍起点距离、背鳍起点到腹鳍起点距离、背鳍起点到臀 鳍起点距离、背鳍末端到尾鳍背部起点距离、尾鳍背部起点到臀鳍起点距离，这 些形态指标可以简单易行的从不同鲤鱼群体中筛选出大头鲤个体；同时通过60 年代、80 年代及2007 年大头鲤的对比，发现大头鲤的外部形态已经发生了很大 变化总体趋势是头部更加宽大，身体更加延长。 最后，利用线粒体控制区序列，并结合Genbank 上的已发布的鲤鱼线粒体控 制区序列，对从星云湖采集的62 尾个体进行了分子系统学研究。结果显示：星 云湖土著鲤鱼已经被大规模非土著鲤鱼入侵，大头鲤仅以杂交品系存在，星云湖 土著鲤鱼面临严重危机，迫切需要进行有效保护。

Invertebrate communitites of lakes Mutanda, Mulehe, Kayumbu and Chahafi and their role in fishery production

Kisoro is a small district (734 km2 ) located in the highland areas of south western Uganda; bordering with Rwanda in the south, Democratic Republic of Congo in the west and Kabale District in the north and the east. The district contains four medium- to- small lakes namely: Mutanda (26.4 km2 ), Mulehe (4.1 km\ Kayumbu (2.2 km2) and Chahafi 1.0 km2). These lakes support small subsistence fisheries for a largely agricultural local population. They are, therefore, locally important as a source of animal (fish) protein, income and employment to the riparian human communities. The fish species include tilapiine fishes: Oreochromis niloticus, 0. leucostictus, Tilapia zillii; Clarias carsoni (Nsonzi), Barbus spp, Cyprinus carpio (Common carp) and the red shrimps

鲤病毒病原的感染性测定

为查明养殖鲤(Cyprinus carpio)突然大批发病死亡的原因,对鲤病样品进行了细胞攻毒、空斑测定、电镜观察,以及鱼体感染等实验。先以患病鲤的组织匀浆液,经过滤后,分别接种到草鱼鳍条细胞(GCF)、鲤上皮瘤细胞(EPC)等14种培养细胞中。利用倒置显微镜观察显示,在1~2 d内,该病鱼组织匀浆液可使其中9种细胞出现典型的细胞病变。收集出现病变的细胞液(即病毒悬液),进一步进行病毒滴度检测、空斑测定和鱼体感染实验。结果显示,在GCF细胞上的病毒滴度为107.3TCID50/mL;在FHM,TSB和GC

鲤鱼3个亚种线粒体DNA ND5/6区段的PCR-RFLP分析及亚种间分子标记的确立

从鲤鱼3个亚种(Cyprinus carpio carpio,Cyprinus carpio haematopterus和Cyprinus carpiorubrofuscus)中选出具代表性的品系共10个,运用PCR方法,扩增出2.4 kb的mtDNA ND5/6区段.共用9种限制性内切酶进行酶切分析,结果表明,每个亚种拥有一种单倍型,有4种限制性内切酶(Dde I,Hae III,Taq I和Mbo I)酶切后产生了能将3个亚种区分开来的诊断性限制性酶切位点.可利用其作为鉴定3个亚种的遗传标记和遗传育种

鱼类转基因早期胚胎细胞的核移植研究

像许多转基因高等生物一样,用显微注射方法研制的转基因鱼均为转植基因嵌合体,培育纯合转基因品系已成为研究者们向往和追求的目标。以澎泽鲫(Carassius auratus,Pengze var.)和黄河鲤(Cyprinus carpio,Huanghe var.)为实验材料,以转基因囊胚细胞核或原肠胚细胞核为供体,进行了细胞核移植.检测了转植基因在所获得的移核胚胎和5尾澎泽鲫移核鱼及1尾黄河鲤移核鱼中的存在状况,分析了用转基因早期胚胎细胞核移植方法研制纯合转基因鱼的可能性。

人生长激素和转移的人生长激素基因对去垂体鱼的生长代偿效应

通过显微注射技术,将小鼠重金属螯合蛋白(MT-1)基因启动顺序与人生长激素基因顺序的重组体pMThGH注入鲤鱼(Cyprinus carpio)的受精卵内,由此发育的转基因鱼及其后代F1和F2均显示出快速生长效应。去垂体后,转基因鲤鱼F2持续生长,而非转基因鲤鱼和鲫鱼(Carassius auratus)的生长停止。给去垂体的鲫鱼腹腔注射生物合成的人生长激素(hGH),可恢复其生长。实验结果表明,转基因鱼体内表达和体外生物合成的hGH均能代偿鲤鱼和鲫鱼的内源生长激素并刺激去垂体鱼的生长。