Evaluating genetic traceability methods for captive-bred marine fish and their applications in fisheries management and wildlife forensics

Growing demands for marine fish products is leading to increased pressure on already depleted wild populations and a rise in aquaculture production. Consequently, more captive-bred fish are released into the wild through accidental escape or deliberate releases. The increased mixing of captive-bred and wild fish may affect the ecological and/or genetic integrity of wild fish populations. Unambiguous identification tools for captive-bred fish will be highly valuable to manage risks (fisheries management) and tracing of escapees and seafood products (wildlife forensics). Using single nucleotide polymorphism (SNP) data from captive-bred and wild populations of Atlantic cod Gadus morhua L. and sole Solea solea L., we explored the efficiency of population and parentage assignment techniques for the identification and tracing of captive-bred fish. Simulated and empirical data were used to correct for stochastic genetic effects. Overall, parentage assignment performed well when a large effective population size characterized the broodstock and escapees originated from early generations of captive breeding. Consequently, parentage assignments are particularly useful from a fisheries management perspective to monitor the effects of deliberate releases of captive-bred fish on wild populations. Population assignment proved to be more efficient after several generations of captive breeding, which makes it a useful method in forensic applications for well-established aquaculture species. We suggest the implementation of a case-by-case strategy when choosing the best method.

Observations on some birds procured near Charleston, South Carolina

This document contains notes on eight species of birds observed by Arthur T. Wayne in Charleston, South Carolina including the Horned Grebe, the Yellow-crowned Night Heron, Bachman’s Warbler, the Rocky Mountain Orange-crowned Warbler, the Cape May Warbler, the Black-poll Warbler, the Red-breasted Nuthatch, and the Blue-gray Gnatcatcher.

Notes on seven birds taken near Charleston, South Carolina

This document contains notes on seven species of birds observed by Arthur T. Wayne in Charleston, South Carolina including the Yellow-bellied Flycatcher, the Purple Martin, the Black-throated Blue Warbler, Cairn’s Warbler, Grinnell’s Water-Thrush, the Kentucky Warbler, and the Mockingbird.

Migração de ligações Wi-Fi não seguras para ligações seguras, após autenticação em captive portal

Atualmente a popularidade das comunicações Wi-Fi tem crescido, os utilizadores acedem a partir de vários dispositivos como telemóveis, tablets, computadores portáteis sendo estes utilizados por qualquer pessoa nos mais variados locais. Com esta utilização massiva por parte dos utilizadores surgiram os hotspots Wi-Fi públicos (em aeroportos, estações de comboios, etc) que permitem a ligação de clientes recorrendo a ligações wireless não seguras (ou abertas). Tais hotspots utilizam, após a ligação de um cliente, um captive portal que captura o tráfego IP com origem no cliente e o redireciona para uma página Web de entrada. A página Web permite ao cliente comprar tempo de acesso à Internet ou, caso já seja um cliente da empresa, autenticar-se para ter acesso à Internet. A necessidade da ligação aberta assenta na possibilidade do operador do hotspot vender acesso à Internet a utilizadores não conhecidos (caso contrário teria de fornecerlhes uma senha previamente). No entanto, fornecer um acesso à Internet wireless sem qualquer tipo de segurança ao nível físico permite que qualquer outro utilizador consiga obter informação sobre a navegação Web dos utilizadores ligados (ex.: escuta de pedidos DNS). Nesta tese pretende-se apresentar uma solução que estenda um dos atuais mecanismos de autenticação Wi-Fi (WPA, WPA2) para que permita, após autenticação em captive portal, a migração de uma ligação aberta para uma ligação segura.

Distribution of the entodiniomorphid ciliate Troglocorys cava Tokiwa, Modrý, Ito, Pomajbíková, Petrželková, & Imai, , (Entodiniomorphida: Blepharocorythidae) in wild and captive chimpanzees.

Trophozoites of Troglocorys cava were detected in all but one of the wild chimpanzee populations from Rubondo Island (Tanzania), with a prevalence ranging between 20% and 78%. However, the ciliate was absent in all captive groups. Prevalence appeared to increase with the number of sequential samples taken from a particular individual and reached 95.5% in wild individuals sampled at least 4 times.

Mitochondrial uncoupling as a regulator of life-history trajectories in birds: an experimental study in the zebra finch.

Mitochondria have a fundamental role in the transduction of energy from food into ATP. The coupling between food oxidation and ATP production is never perfect, but may nevertheless be of evolutionary significance. The 'uncoupling to survive' hypothesis suggests that 'mild' mitochondrial uncoupling evolved as a protective mechanism against the excessive production of damaging reactive oxygen species (ROS). Because resource allocation and ROS production are thought to shape animal life histories, alternative life-history trajectories might be driven by individual variation in the degree of mitochondrial uncoupling. We tested this hypothesis in a small bird species, the zebra finch (Taeniopygia guttata), by treating adults with the artificial mitochondrial uncoupler 2,4-dinitrophenol (DNP) over a 32-month period. In agreement with our expectations, the uncoupling treatment increased metabolic rate. However, we found no evidence that treated birds enjoyed lower oxidative stress levels or greater survival rates, in contrast to previous results in other taxa. In vitro experiments revealed lower sensitivity of ROS production to DNP in mitochondria isolated from skeletal muscles of zebra finch than mouse. In addition, we found significant reductions in the number of eggs laid and in the inflammatory immune response in treated birds. Altogether, our data suggest that the 'uncoupling to survive' hypothesis may not be applicable for zebra finches, presumably because of lower effects of mitochondrial uncoupling on mitochondrial ROS production in birds than in mammals. Nevertheless, mitochondrial uncoupling appeared to be a potential life-history regulator of traits such as fecundity and immunity at adulthood, even with food supplied ad libitum.

Bionomics, cryopreservation of gametes and captive breeding behaviour of threatened hillstream cyprinid, garra surendranathanii(shaji, arun & easa, 1996)

Garra surendranathanii is a hill stream cyprinid endemic to Kerala. According to IUCN based classification, G. surendranathanii is grouped under the threatened category. This endemic fish is having highly restricted and fragmented distribution and reported only from 5 river systems viz. Chalakudy, Periyar, Pamba, Achenkoil and Bharathapuzha. Categorization of this fish as a potential ornamental candidate can invariably add more pressure on the threat status of this particular species. Hence, this species is considered as one which requires foremost attention for conservation. Hitherto, no infomiation is available on the bionomics, resource characteristics and any conservation attempts of G. surendranathanii. Studies on detailed life history traits and development of captive breeding technique are indispensable for successful fishery management.The present study was undertaken with the following obj ectives: To study the Length-weight relationship and condition factor to ascertain the relationship between length and weight and general wellbeing ofthe fish To study the age and growth to understand the age composition of the exploited stock, age at first maturation and life span of the species. To study the reproductive biology of G. surendranathanii to gain insights in the process of gametogenesis, spawning, sex ratio, fecundity and other related aspects which are essential for developing captive breeding technology of this species. To develop captive breeding technology and cryopreservation ofgametes of G. surendranathanii for conservation

Birds.

Tiene como objeto explorar los pájaros y ofrecer respuestas a muchos de los qué, por qué y cómo que los niños pequeños preguntan sobre el mundo que les rodea. Para ayudar a los niños a entenderpor qué los pájaros tienen plumas,cómo las aves migratorias encuentran su camino. Cada dos páginas hay tres secciones organizadas por temas:un Ahora sé, es una revisión de los principales aspectos que refuerza lo aprendido. Eso es asombroso, ofrece características destacadas de la costa. Mira y encuentra sirve para alentar a los niños a identificar y asociar nombres con imágenes.

Can salvage logging affect seed dispersal by birds into burned forests?

The recovery of vegetation in Mediterranean ecosystems after wildfire is mostly a result of direct regeneration, since the same species existing before the fire regenerate on-site by seeding or resprouting. However, the possibility of plant colonization by dispersal of seeds from unburned areas remains poorly studied. We addressed the role of the frugivorous, bird-dependent seed dispersal (seed rain) of fleshy-fruited plants in a burned and managed forest in the second winter after a fire, before on-site fruit production had begun. We also assessed the effect on seed rain of different microhabitats resulting from salvage logging (erosion barriers, standing snags, open areas), as well as the microhabitats of unlogged patches and an unburned control forest, taking account of the importance of perches as seed rain sites. We found considerable seed rain by birds in the burned area. Seeds, mostly from Olive trees Olea europaea and Evergreen pistaches Pistacia lentiscus, belonged to plants fruiting only in surrounding unburned areas. Seed rain was heterogeneous, and depended on microhabitat, with the highest seed density in the unburned control forest but closely followed by the wood piles of erosion barriers. In contrast, very low densities were found under perches of standing snags. Furthermore, frugivorous bird richness seemed to be higher in the erosion barriers than elsewhere. Our results highlight the importance of this specific post-fire management in bird-dependent seed rain and also may suggest a consequent heterogeneous distribution of fleshy-fruited plants in burned and managed areas. However, there needs to be more study of the establishment success of dispersed seeds before an accurate assessment can be made of the role of bird-mediated seed dispersal in post-fire regeneration

Blood transfusion in birds

Birds, both companion and wild, have become frequent patients in veterinary clinics and hospitals as well in wildlife rehabilitation centers in the last two decades. In emergency situations, it is imperative to give the best critical care to these patients, which frequently implies reversing shock and re-establishing homeostasis. In order to do so, a blood transfusion can be life-saving. It is important to the clinician to properly evaluate these critical patients and decide when to administer blood or blood products.