Detection of Copper Ion with Laser-Induced Fluorescence in A Capillary Electrophoresis Microchip

A capillary electrophoresis microchip coupled with a confocal laser-induced fluorescence (LIF) detector was successfully constructed for the analysis of trace amounts of heavy metals in environmental sources. A new fluorescence dye, RBPhOH, synthesized from rhodamine B, was utilized in a glass microchip to selectively determine copper with high sensitivity. A series of factors including running buffer concentration, detection voltage, and sample loading time were optimized for maximum LIF detector response and, hence, method sensitivity.

A study of the influence of initial liquid volume on the capillary flow in an interior corner under microgravity

In this work the influence of initial liquid volume on the capillary flow in an interior corner is studied systematically by microgravity experiments using the drop tower, under three different conditions: the Concus-Finn condition is satisfied,close to and dissatisfied. The capillary flow is studied by discussing the movement of tip of the meniscus in the corner. Experimental results show that with the increase of initial liquid volume the tip location increases for a given microgravity time, the achievable maximum tip velocity increases and the flow reaches its maximum tip velocity earlier However, the results for the three different conditions show some difference. (C) 2010 Elsevier Ltd All rights reserved

Hot Nuclear Matter Equation of State and Finite Temperature Kaon Condensation

We perform a systematic calculation of the equation of state of asymmetric nuclear matter at finite temperature within the framework of the Brueckner-Hartree-Fock approach with a microscopic three-body force. When applying it to the study of hotka on condensed matter, we find that the thermal effect is more profound in comparison with normal matter, in particular around the threshold density. Also, the increase of temperature makes the equation of state slightly stiffer through suppression of kaon condensation.

A correlation between radiation sensitivity and initial chromatid breaks in cancer cell lines revealed by Calyculin A-induced premature condensation

Three human malignancy cell lines were irradiated with Co-60 gamma-rays. Initial chromatid breaks were measured by using the chemically induced premature chromosome condensation technique. Survival curves of cells exposed to gamma rays was linear-quadratic while the efficiency of Calyculin A in inducing PCC of G(2) PCC was about five times more than G(1) PCC. A dose-dependent increase in radiation-induced chromatid/isochromatid breaks was observed in G(1) and G(2) phase PCC and a nearly positive linear correlation was found between cell survival and chromatin breaks. This study implies that low LET radiation-induced chromatid/isochromatid breaks can potentially be used to predict the radiosensitivity of tumor cells either in in vitro experimentation or in in vivo clinical radiotherapy.

Antikaon condensation and in-medium kaon and antikaon production in protoneutron stars

Antikaon condensation and kaon and antikaon production in protoneutron stars are investigated in a chiral hadronic model (also referred to as the FST model in this paper). The effects of neutrino trapping on protoneutron stars are analyzed systematically. It is shown that neutrino trapping makes the critical density of K- condensation delay to higher density and (K) over bar (0) condensation not occur. The equation of state (EOS) of (proto)neutron star matter with neutrino trapping is stiffer than that without neutrino trapping, As a result, the maximum masses of (proto)neutron stars with neutrino trapping are larger than those without neutrino trapping. If hyperons are taken into account, antikaon does not form a condensate in (Proto)neutron stars. Meanwhile, the corresponding EOS becomes much softer, and the maximum masses of (proto)neutron stars are smaller than those without hyprons. Finally, our results illustrate that the Q values for K+ and K- production in (proto)neutron stars are not sensitive to neutrino trapping and inclusion of hyperons.

Microscopic three-body forces and kaon condensation in cold neutrino-trapped matter

We investigate the composition and the equation of state of the kaon condensed phase in neutrino-free and neutrino-trapped star matter within the framework of the Brueckner-Hartree-Fock approach with three-body forces. We find that neutrino trapping shifts the onset density of kaon condensation to a larger baryon density, and reduces considerably the kaon abundance. As a consequence, when kaons are allowed, the equation of state of neutrino-trapped star matter becomes stiffer than the one of neutrino free matter. The effects of different three-body forces are compared and discussed. Neutrino trapping turns out to weaken the role played by the symmetry energy in determining the composition of stellar matter, and thus reduces the difference between the results obtained by using different three-body forces.

Phase transition to color-flavor-locked matter and condensation of Goldstone bosons in neutron star matter

Deconfinement phase transition and condensation of Goldstone bosons in neutron star matter are investigated in a chiral hadronic model (also referred as to the FST model) for the hadronic phase (HP) and in the color-flavor-locked (CFL) quark model for the deconfined quark phase. It is shown that the hadronic-CFL mixed phase (MP) exists in the center of neutron stars with a small bag constant, while the CFL quark matter cannot appear in neutron stars when a large bag constant is taken. Color superconductivity softens the equation of state (EOS) and decreases the maximum mass of neutron stars compared with the unpaired quark matter. The K-0 condensation in the CFL phase has no remarkable contribution to the EOS and properties of neutron star matter. The EOS and the properties of neutron star matter are sensitive to the bag constant B, the strange quark mass m(s) and the color superconducting gap Delta. Increasing B and m(s) or decreasing Delta can stiffen the EOS which results in the larger maximum masses of neutron stars.

Comparison of clonogenic assay with premature chromosome condensation assay in prediction of human cell radiosensitivity

Aim: To determine whether the number of non-rejoining G2-chromatid breaks can predict the radiosensitivity of human cell lines. Methods: Cell lines of human ovary carcinoma cells (HO8910), human hepatoma cells (HepG2) and liver cells (L02) were irradiated with a range of doses and assessed both of cell survival and non-rejoining G2-chromatid breaks at 24 h after irradiation. Cell survival was documented by a colony assay. Non-rejoining G2-chromatid breaks were measured by counting the number of non-rejoining G2 chromatid breaks at 24 h after irradiation, detected by the prematurely chromosome condensed (PCC) technique. Results: A linear-quadratic survival curve was observed in three cell lines, and HepG2 was the most sensitive to gamma-radiation. A dose-dependent linear increase was observed in radiation-induced non-rejoining G2-PCC breaks measured at 24 h after irradiation in all cell lines, and HepG2 was the most susceptible to induction of non-rejoining G2-PCC breaks. A close correlation was found between the clonogenic radiosensitivity and the radiation-induced non-rejoining G2-PCC breaks (r=0.923). Furthermore, survival-aberration correlations for two or more than two doses lever were also significant. Conclusion: The number of non-rejoining G2 PCC breaks holds considerable promise for predicting the radiosensitivity of normal and tumor cells when two or more than two doses lever is tested.

Antikaon condensation and deconfinement phase transition in neutron stars

Antikaon condensation and deconfinement phase transition in neutron stars are investigated in a chiral hadronic model (also referred as to the FST model) for the hadronic phase and in the MIT bag model for the deconfined quark matter phase. It is shown that the existence of quark matter phase makes antikaon condensation impossible in neutron stars. The properties of neutron stars are sensitive to the bag constant. For the small values of the bag constant, the pure quark matter core appears and hyperons are strongly suppressed in neutron stars, whereas for the large bag constant, the hadron-quark mixed phase exists in the center of neutron stars. The maximum masses of neutron stars with the quark matter phase are lower than those without the quark matter phase; meanwhile, the maximum masses of neutron stars with the quark matter phase increase with the bag constant.

An etched porous interface for on-line capillary electrophoresis-based two-dimensional separation system

The construction and evaluation of an on-column etched fused-silica porous junction for on-line coupling of capillary isoelectric focusing (CIEF) with capillary zone electrophoresis (CZE) are described. Where two separation columns were integrated on a single piece of fused-silica capillary through the etched similar to4 to 5-mm length porous junction along the capillary. The junction is easily prepared by etching a short section of the capillary wall with HF after removing the polyimide coating. The etched section becomes a porous glass membrane that allows only small ions related to the background electrolyte to pass through when high voltage is applied across the separation capillary. The primary advantages of this novel porous junction interface over previous designs (in which the interface is usually formed by fracturing the capillary followed by connecting the two capillaries with a section of microdialysis hollow fiber membrane) are no dead volume, simplicity, and ruggedness, which is particularly well suited for an on-line coupling capillary electrophoresis-based multiple dimensional separation system. The performance of the 2D CIEF-CZE system constructed by such an etched porous junction was evaluated by the analyses of protein mixtures.

Recent advances in the study of biomolecular interactions by capillary electrophoresis

Capillary electrophoresis (CE) has been abundantly used in the study of molecular interactions owing to such advantages as short analysis time, low sample size requirement, high separation efficiency, and flexible applications. The focus of this paper is to 2 review recent studies and advances (mainly from 1998 to now) in biomolecular interactions using CE. Five CE modes: zone migration CE, affinity CE, frontal analysis (FA), Hummel-Dreyer (HD) and vacancy peak (VP) are cited and compared. Quantitative aspects of the thermodynamics and kinetics of biomolecular interaction are reviewed. Several biomolecular binding systems, including protein-protein (polypeptide), protein-DNA (RNA), protein(polypeptide)-carbohydrate, protein-small molecule, DNA-small molecule, small molecule-small molecule, have been well characterized by CE. CE is shown to be a powerful tool for the determination of the binding parameters of various bioaffinity interactions.