Analysis and functional annotation of an expressed sequence tag collection for tropical crop sugarcane

To contribute to our understanding of the genome complexity of sugarcane, we undertook a large-scale expressed sequence tag (EST),program. More than 260,000 cDNA clones were partially sequenced from 26 standard cDNA libraries generated from different sugarcane tissues. After the processing of the sequences, 237,954 high-quality ESTs were identified. These ESTs were assembled into 43,141 putative transcripts. of the assembled sequences, 35.6% presented no matches with existing sequences in public databases. A global analysis of the whole SUCEST data set indicated that 14,409 assembled sequences (33% of the total) contained at least one cDNA clone with a full-length insert. Annotation of the 43,141 assembled sequences associated almost 50% of the putative identified sugarcane genes with protein metabolism, cellular communication/signal transduction, bioenergetics, and stress responses. Inspection of the translated assembled sequences for conserved protein domains revealed 40,821 amino acid sequences with 1415 Pfam domains. Reassembling the consensus sequences of the 43,141 transcripts revealed a 22% redundancy in the first assembling. This indicated that possibly 33,620 unique genes had been identified and indicated that >90% of the sugarcane expressed genes were tagged.

Reproductive responses of rabbit does to a supplemental lighting program

The objective was to investigate whether the productivity of rabbit does can be improved, when natural photoperiod is decreasing, by adopting a supplemental lighting program. Three experiments were conducted involving two groups: control, submitted to the natural decreasing photoperiod, and supplemented with a lighting program which provided 14 h light/24 h beginning at 10 weeks of age. In the first experiment, 20 nulliparous does, 10 from each group, were euthanized 8 h after being presented to a buck; the overall number of follicles, whose diameter exceeded I mm, was determined macroscopically. The right ovaries were collected, histologically analyzed, and electronically measured. In the second experiment, 30 nulliparous does, 15 from each group, were presented to a buck (day 1). Receptive does were euthanized on day 8 to evaluate embryonic survival (number of normal embryos/ovulation rate). In the third experiment, 48 nulliparous does, 24 from each group, were followed from the first presentation to the buck until the weaning of the first litter. The effect of treatment on reproductive and body weight traits of does, and litter performance traits, at birth and weaning, was evaluated. The average number of follicles whose diameter exceeded 1 mm was higher in the treatment group (12.05 +/- 1.07 vs. 8.63 +/- 1.00, P=0.03 7). Receptive does of the treatment group had heavier ovaries relative to those of the control group (790 +/- 59 vs. 470 +/- 64 mg, P=0.004), whereas no treatment difference regarding this trait was found for non-receptive ones. Treatment had a favorable effect on pregnancy rate of total exposed and of receptive does (80.0% vs. 33.3%, P=0.01, and 92.3% vs. 50.0%, P=0.02, respectively). The number of underdeveloped embryos was lower (0.067 +/- 0.380 vs. 2.500 +/- 0.455, P=0.004), embryonic survival up to day 8, and uterus weight was higher in the treatment group (0.839 +/- 0.075 vs. 0.534 +/- 0.087, P=0.033 and 13.83 +/- 0.72 vs. 10.99 +/- 0.84, P=0.037, respectively). Number of presentations tended to be lower (1.32 +/- 0.17 vs. 1.75 +/- 0.16, P=0.077) and adjusted litter size in the first reproductive cycle tended to be higher (7.09 +/- 0.89 vs. 5.22 +/- 0.68, P=0.091) in the treatment group relative to the control.

Green lacewings (Neuroptera: Chrysopidae) associated with Melon Crop in Mossoró, Rio Grande do Norte State, Brazil

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

WATER NEEDS FOR WINTER BEAN CROP

Um estudo sobre o uso de água do feijoeiro de inverno (Phaseolus vulgaris L. cv. Carioca) foi realizado num solo Latossol Vermelho Amarelo de textura argilosa. Um sistema de sulcos de infiltração foi usado para proceder a irrigação com o intuito de manter o solo em potenciais de água superiores a -40,0 KPa. Duas doses de aplicação de N em cobertura (0 a 30 Kg N/ha) foram colocados 25 dias após o plantio. Os principais objetivos do estudo foram: avaliar a interação entre as duas doses de N com a evapotranspiração e medir os coeficientes de cultura (Kc). A evapotranspiração média máxima (ETm) foi 1,71 mm/dia, ou 157,16 mm nos 92 dias de observações; os valores de ETm para as fases vegetativa (1), florescimento (2) e formação de vagens (3) foram 1,48; 2,35 e 1,50 mm/dia, respectivamente, para a dose de 30 Kg/ha e 1,48, 1,88 e 1,45 mm/dia para o tratamento sem aplicação de N em cobertura. Os coeficientes de cultura (Kc = ETm/ETo) foram 0,62 e 0,78 para a fase 1, 0,80 e 1,10 para a 2, 0,45 e 0,55 para a 3 e 0,61 e 0,80 para o ciclo todo, respectivamente, baseados no método de FAO-Penman e do Tanque Classe A. Este mostrou melhores resultados para estimar o máximo uso de água pelo feijoeiro de inverno. Os tratamentos de N não afetaram a evapotranspiração significativamente. Entretanto, a evapotranspiração, medida pelo método do balanço de água, foi 59,78 e 27,12% maior no estágio do florescimento que no estádio vegetativo, respectivamente, nas doses de 30 e 0 Kg N/ha.

An Innovative Crop-Forage Intercrop System: Early Cycle Soybean Cultivars and Palisadegrass

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Potential of Brazilian eugenia myrtaceae - As ornamental and as a fruit crop

Brazilian Myrtaceae comprises several genera of trees and shrubs used for ornamental and fruit production. In addition to the well known Guava, Pitanga and Jaboticaba, other species could be used for fruitculture, due the value and quality of their fruits and adaptation to some climate conditions mainly the subtropical one. Nine species of Eugenia were evaluated at Jaboticabal, located at 48° W and 21° S in São Paulo state in a germplasm bank. The average rain by year is 1431 mm and the temperature 22,2° C at an altitude of 575 m. The species are Eugenia klozschiana Berg. (Pero-do-campo), E. stipitata Mc Vaugh (Araça-boi), E. tomentosa Camb. (Cabeludinha), E. dysentherica DC. (Cagaita), E. brasiliensis Berg. (Grumixama), E.pitanga (Pitanga-anã), E. luchsnathiana Berg. (Pitomba), E. uvalha Camb. (Uvaia) and E. involucrata DC. (Cereja-do-rio-grande). The evaluations comprised tree development, fruit quality and leaf and flower morphological studies. The main results are: the trees of Pera-do-campo and Pitanga-anã are small shrubs of 1 to 2 m height, Araça-boi and Cabeludinha are small trees, 3 to 5 m high, and the other species are tall trees, with 5 to 10 m height. The species adapted well to the subtropical conditions, except for Araça-boi, which is native to the Amazonian region and exhibited a severe fungus disease infection. In relation to fruit quality, all the species had edible fruit, some were sweet and juicy, Cabeludinha, Grumixama, Pitomba, Cereja-do-rio grande and Pitanga-anã, while others had high acidity (Araça-boi, Pera-do-campo, Cagaita and Uvaia and were more suitable for processing. Simple, single leaves were characteristic of all species, but with different sizes and shapes., With the addition of color, smell and other characteristics, leaf size and shape were useful for comparative classification. Flower components and structure are described also.

A genetic algorithm for crop rotation

In the last few years, crop rotation has gained attention due to its economic, environmental and social importance which explains why it can be highly beneficial for farmers. This paper presents a mathematical model for the Crop Rotation Problem (CRP) that was adapted from literature for this highly complex combinatorial problem. The CRP is devised to find a vegetable planting program that takes into account green fertilization restrictions, the set-aside period, planting restrictions for neighboring lots and for crop sequencing, demand constraints, while, at the same time, maximizing the profitability of the planted area. The main aim of this study is to develop a genetic algorithm and test it in a real context. The genetic algorithm involves a constructive heuristic to build the initial population and the operators of crossover, mutation, migration and elitism. The computational experiment was performed for a medium dimension real planting area with 16 lots, considering 29 crops of 10 different botanical families and a two-year planting rotation. Results showed that the algorithm determined feasible solutions in a reasonable computational time, thus proving its efficacy for dealing with this practical application.

Índice de seleção bioeconômico para fêmeas de corte da raça nelore

The reproductive efficiency of Nellore females was described on the basis of sexual precocity, staying productive in the herd (NP), maternal productivity (PM) and estimated cost for maintenance (CM). The combination of these traits resulted in the bioeconomic maternal revenue index (RMat) ensures reproductive efficiency. The index estimates the return in kilograms of live weight produced per cow per year. In addition, the composition of calf weight produced was considered, adding to the PM the information on the scores of conformation, muscling and early finishing at weaning, in order to input the calf biotype. The females considered precocious had their age at first calving before 30 months. The NP was expressed by the number of calving till 53 months of age. The CM was calculated according to the estimated feed intake of dry matter. The estimated average RMat was 62.02±24.12 kg/ cow/year. Estimates of additive genetic and residual variances for RMat, using restricted maximum likelihood under a single trait animal model was equal to 195.35 and 242.96, respectively. The heritability estimated was 0.45±0.02, indicating that RMat is inheritable and can be applied in the breeding program to improve reproductive efficiency. The NP was the main component of variation for RMat. Sires selected based on RMat tended to have more efficient daughters.

Genetic associations among average annual productivity, growth traits, and stayability: A parallel between Nelore and composite beef cattle

This study was conducted to examine the relationship among average annual productivity of the cow (PRODAM), yearling weight (YW), postweaning BW gain (PWG), scrotal circumference (SC), and stayability in the herd for at least 6 yr (STAY) of Nelore and composite beef cattle. Measurements were taken on animals born between 1980 and 2010 on 70 farms located in 7 Brazilian states. Estimates of heritability and genetic and environmental correlations were obtained by Bayesian approach with 5-trait animal models. Genetic trends were estimated by regressing means of estimated breeding values by year of birth. The heritability estimates were between 0.14 and 0.47. Estimates of genetic correlation among female traits (PRODAM and STAY) and growth traits ranged from-0.02 to 0.30. Estimates of genetic correlations ranged from 0.23 to 0.94 among growth traits indicating that selection for these traits could be successful in tropical breeding programs. Genetic correlations among all traits were favorable and simultaneous selection for growth, productivity, and stayability is therefore possible. Genetic correlation between PRODAM and STAY was 0.99 and 0.85 for Nelore and composite cattle, respectively. Therefore, PRODAM and STAY might be influenced by many of the same genes. The inclusion of PRODAM instead of STAY as a selection criterion seems to be more advantageous for tropical breeding programs because the generation interval required to obtain accurate estimates of genetic merit for PRODAM is shorter. Average annual genetic changes were greater in Nelore than in composite cattle. This was not unexpected because the breeding program of composite cattle included a large number of farms, different production environments, and genetic level of the herds and breeds. Thus, the selection process has become more difficult in this population. © 2013 American Society of Animal Science. All rights reserved.

Sorghum grain yield, forage biomass production and revenue as affected by intercropping time

Sorghum is an excellent alternative to other grains in poor soil where corn does not develop very well, as well as in regions with warm and dry winters. Intercropping sorghum [Sorghum bicolor (L.) Moench] with forage crops, such as palisade grass [Brachiaria brizantha (Hochst. ex A. Rich) Stapf] or guinea grass (Panicum maximum Jacq.), provides large amounts of biomass for use as straw in no-tillage systems or as pasture. However, it is important to determine the appropriate time at which these forage crops have to be sown into sorghum systems to avoid reductions in both sorghum and forage production and to maximize the revenue of the cropping system. This study, conducted for three growing seasons at Botucatu in the State of São Paulo in Brazil, evaluated how nutrient concentration, yield components, sorghum grain yield, revenue, and forage crop dry matter production were affected by the timing of forage intercropping. The experimental design was a randomized complete block design. Intercropping systems were not found to cause reductions in the nutrient concentration in sorghum plants. The number of panicles per unit area of sorghum alone (133,600), intercropped sorghum and palisade grass (133,300) and intercropped sorghum and guinea grass (134,300) corresponded to sorghum grain yields of 5439, 5436 and 5566kgha-1, respectively. However, the number of panicles per unit area of intercropped sorghum and palisade grass (144,700) and intercropped sorghum and guinea grass (145,000) with topdressing of fertilizers for the sorghum resulted in the highest sorghum grain yields (6238 and 6127kgha-1 for intercropping with palisade grass and guinea grass, respectively). Forage production (8112, 10,972 and 13,193Mg ha-1 for the first, second and third cuts, respectively) was highest when sorghum and guinea grass were intercropped. The timing of intercropping is an important factor in sorghum grain yield and forage production. Palisade grass or guinea grass must be intercropped with sorghum with topdressing fertilization to achieve the highest sorghum grain yield, but this significantly reduces the forage production. Intercropping sorghum with guinea grass sown simultaneously yielded the highest revenue per ha (€ 1074.4), which was 2.4 times greater than the revenue achieved by sowing sorghum only. © 2013 Elsevier B.V.

Avaliação de híbridos de mamona de porte baixo em espaçamento reduzido em condições de safrinha

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Alterações no número de cópias genômicas em mastocitomas cutâneos caninos

Pós-graduação em Medicina Veterinária - FCAV

Produtividade do feijoeiro sob supressão de irrigação em diferentes fases fenológicas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

An assessment of the economic impact of climate change on the agriculture sector in Trinidad And Tobago

The economic impact of climate change on root crop, fisheries and vegetable production for Trinidad and Tobago under the A2 and B2 scenarios were modeled, relative to a baseline ―no climate change‖ case, where the mean temperature and rainfall for a base period of 1980 – 2000 was assumed for the years up to 2050. Production functions were used, using ARMA specifications to correct for serial autocorrelation. For the A2 scenarios, rainfall is expected to fall by approximately 10% relative to the baseline case in the 2020s, but is expected to rise thereafter, until by the 2040s rainfall rises slightly above the mean for the baseline case. For the B2 scenario, rainfall rose slightly above the mean for the baseline case in the current decade, but falls steadily thereafter to approximately 15% by the 2040s. Over the same period, temperature is expected to increase by 1.34C and 1.37C under A2 and B2 respectively. It is expected that any further increase in rainfall should have a deleterious effect on root crop production as a whole, since the above mentioned crops represent the majority of the root crops included in the study. Further expected increases in temperature will result in the ambient temperature being very close to the optimal end of the range for most of these crops. By 2050, the value of yield cumulative losses (2008$) for root crops is expected to be approximately 248.8 million USD under the A2 scenario and approximately 239.4 million USD under the B2 scenario. Relative to the 2005 catch for fish, there will be a decrease in catch potential of 10 - 20% by 2050 relative to 2005 catch potentials, other things remaining constant. By 2050 under the A2 and B2 scenarios, losses in real terms were estimated to be 160.2 million USD and 80.1 million USD respectively, at a 1% discount rate. For vegetables, the mean rainfall exceeds the optimal rainfall range for sweet peppers, hot peppers and melongene. However, while the optimal rainfall level for tomatoes is 3000mm/yr, other vegetables such as sweet peppers, hot peppers and ochroes have very low rainfall requirements (as low as 300 mm/yr). Therefore it is expected that any further decrease in rainfall should have a mixed effect on individual vegetable production. It is expected that any further increase in temperature should have a mixed effect on individual vegetable production, though model results indicated that as a group, an increase in temperature should have a positive impact on vegetable production. By 2050, the value of yield cumulative gains (2008$) for vegetables is expected to be approximately 54.9 million USD under the A2 scenario and approximately 49.1 million USD under the B2 scenario, given a 1% discount rate. For root crops, fisheries and vegetables combined, the cumulative loss under A2 is calculated as approximately 352.8 million USD and approximately 270.8 million USD under B2 by 2050. This is equivalent to 1.37% and 1.05% of the 2008 GDP under the A2 and B2 scenarios respectively by 2050. Sea Level Rise (SLR) by 2050 is estimated to be 0.255 m under A2 and 0.215 m under B2. GIS estimation indicated that for a 0.255 m sea level rise, combined with a 0.5 m high tide, there would be no permanent inundation of agricultural land in Trinidad. The total inundation area is 1.18 km2. This occurs only in the Caroni Watershed, on the western coast of Trinidad, and the areas are outside the Caroni Swamp. Even with an additional rise of 0.5 m to simulate a high rainfall event, the estimated inundated area is 4.67 km2, but with no permanent inundation, though likely to be subject to flooding. Based on eleven (11) evaluation criteria, the top potential adaptation options were identified: 1. Use of water saving irrigation systems and water management systems e.g. drip irrigation; 2. Mainstream climate change issues into agricultural management; 3. Repair/maintain existing dams; 4. Alter crop calendar for short-term crops; 5. Adopt improved technologies for soil conservation; 6. Establish systems of food storage; 7. Promote water conservation – install on-farm water harvesting off roof tops; 8. Design and implement holistic water management plans for all competing uses; 9. Build on- farm water storage (ponds and tanks); 10. Agricultural drainage; and 11. Installation of greenhouses. The most attractive adaptation options, based on the Benefit-Cost Ratio are: (1) Build on- farm water storage such as ponds and tanks (2) Mainstreaming climate change issues into agricultural management and (3) Water Harvesting. However, the options with the highest net benefits are, (in order of priority): (1) Build on- farm water storage such as ponds and tanks, (2) Mainstreaming climate change issues into agricultural management and (3) Use of drip irrigation. Based on the area burnt in Trinidad and Tobago between 2005 and 2009, the average annual loss due to fires is 1717.3 ha. At US$17.41 per carbon credit, this implies that for the total land lost to forest fires on average each year, the opportunity cost of carbon credit revenue is 74.3 million USD. If a teak reforestation programme is undertaken in Trinidad and Tobago, the net benefit of reforestation under a carbon credit programme would be 69 million USD cumulatively to 2050.

Capacidade combinatória de linhagens de milho selecionadas para sistema radicular

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)