Proteinograma do líquido sinovial de equinos hígidosobtido por eletroforese em gel de poliacrilamida

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Avaliação do potencial efeito cicatrizante gástrico e antiartrítico da mangiferina, uma xantona glicosilada da Mangifera indica

Rheumatoid arthritis (RA) is a chronic illness with great potential to cause comorbidities resulting from cumulative inflammatory activities, and it contributes with the increase of disabilities and death of patients. It affects 1 to 2% of world population and usually occurs between 30 and 50 years of age. Among existing therapeutic options for the disease non-steroidal anti-inflammatory drugs (NSAIDs) still play an important part in the treatment, being widely used by patients to relieve pain and stiffness. However, this class of drugs causes many adverse gastrointestinal effects, such as dyspepsia, heartburn, nausea and vomit, and its use is one of the most common causes of peptic ulcers. Mangiferin (a glicosilated xanthone extracted mainly from the bark of Mangifera indica L.) is the main compound of an aqueous extract made from the bark stem of the mango tree. Previous studies conducted by our research group prove the anti-inflammatory action of mangiferin on an animal model of periodontitis, and its gastroprotective action has been described before. Considering these informations this study evaluated mangiferin’s potential on the treatment of RA and on gastric ulcer healing in animal models, and analyzed toxicity parameters to assure efficacy and safety of the compound as potential new drug for the treatment of the disease. RA was induced in rats by subcutaneous injection of bovine collagen and Freund’s complete adjuvant. This method presented low incidence of RA in rats, but we were able to induce the disease in 60 to 70% of the animals. Due to the wide use of NSAIDs and its potential to cause peptic ulcers, we induced gastric ulcer on arthritic rats to analyze mangiferin’s gastric healing effect. After 14 days of treatment we noticed small increase of the lesion area of animals treated with mangiferin or ibuprofen, when compared to the animals... (Complete abstract click electronic access below)

Avaliação da toxicidade do cloreto de níquel utilizando Allium cepa como organismo teste

O impacto das atividades antropogênicas sobre o meio ambiente é um tema de grande relevância atualmente, atraindo a atenção da comunidade científica em geral, visto que essas atividades têm causado alterações significativas na qualidade da água, do solo e do ar. Dentre a ampla gama de poluentes resultantes das atividades humanas, merece destaque a classe dos metais pesados, os quais possuem propriedades tóxicas e genotóxicas, constituindo fatores de risco para o desenvolvimento de desordens neurodegenerativas, artrite e câncer nas populações expostas a estes contaminantes, bem como alterações nos ecossistemas contaminados. Neste sentido, o presente trabalho teve por objetivo avaliar o potencial citotóxico, genotóxico e mutagênico do níquel como contaminante, utilizando Allium cepa (Liliaceae) como organismo-teste, a fim de detectar possíveis efeitos deletérios em seu material genético. Estas avaliações foram feitas por meio da exposição de sementes de A. cepa a três soluções, com diferentes concentrações de níquel. Os resultados obtidos no primeiro biensaio e na repetição estão apresentados na forma de artigo científico, a ser submetido para revista especializada. O estudo dos efeitos do níquel em A. cepa foram importantes, uma vez que os dados da avaliação dos danos induzidos neste organismo modelo podem ser utilizados para a comparação com modelos animais, sendo possível assim, a inferência dos perigos aos ecossistemas e à população que está exposta a esse contaminante

Leishmaniose visceral canina: estudo imagiológico em cães naturalmente infectados

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Compostos antioxidantes e sua importância nos organismos

A large amount of external factors that can foster the emergence of free radicals in organisms has created a imminent need for understanding and technologies that enables the search of antioxidant componds and extraction of antioxidants from natural sources. These compounds have great potential for kidnapping free radicals which are highly harmful to organisms, being the principal responsible for the decrease in the shelf life of foods, and aging. These free radicals are also related to diseases such as AIDS, arthritis, diabetes, heart disease and others. Fruits and vegetables are true mines of compounds with antioxidant potential, its vitamins and phenolic compounds present an essential role in the nutrition of living beings acting as a defense against degradation of lipids and proteins, which indicates the importance of a diet rich in these nutrients, given that part of the antioxidant defenses of the body comes from the diet. This revision study aims to put in evidence the indispensable role of antioxidants in the survival of organisms.

Avaliação da mutagenicidade e da estrogenicidade dos extratos etanólicos padronizados, frações e substâncias isoladas de Arrabidaea brachypoda (DC.) Bureau

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Inquérito sobre a qualidade de vida relacionada à saúde em crianças e adolescentes portadores de artrites idiopáticas juvenis

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Avaliação das atividades cicatrizante e antiinflamatória de Casearia sylvestris Swartz em animais e identificação dos marcadores químicos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Testes preliminares de motilidade intestinal e toxicidade oral aguda com extrato de cascas pulverizadas de Endopleura uchi (Huber) Cuatrec: (Humiriaceae) em camundongos

The aim of this work was to analyze the acute oral toxicity and the effects on intestinal motility of the extract obtained through decoction 20% (m/v) of Endopleura uchi, popularly known as "uxi-amarelo", a native plant from the Brazilian Amazon. The plant is used indiscriminately against several diseases: arthritis, cholesterol, diabetes, ulcers, myomas, and intestinal illnesses in general, among others. The present results show that there were no significant alterations in intestinal motility and that the extract does not present signs of toxicity, showing its safety for consumption purposes.

Avaliação audiológica em pacientes com doenças reumáticas pediátricas

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Produtividade e perfil químico de óleo essencial de acessos de Varronia curassavica Jacq. em diferentes horários de coleta e período sazonal

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Índice de resistência múltipla aos antimicrobianos, concentração inibitória e beta-lactamases de espectro estendido em linhagens de Proteus mirabilis e Proteus vulgaris isoladas de diferentes afecções em animais domésticos

Pós-graduação em Medicina Veterinária - FMVZ

Patofisiologia de osteonecrose induzida por bisfosfonatos e inibidores de RANK-L

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Enhanced phagocytosis of Corynebacterium pseudotuberculosis by monocyte-macrophage cells from goats naturally infected with caprine arthritis encephalitis virus

Sanches B.G.S., Souza F.N., Azedo M.R., Batista C.F., Bertagnon H.G., Blagitz M.G. & Della Libera A.M.M.P. 2012. [Enhanced phagocytosis of Corynebacterium pseudotuberculosis by monocyte-macrophage cells from goats naturally infected with caprine arthritis encephalitis virus.] Fagocitose intensificada de Corynebacterium pseudotuberculosis por celulas da serie monocito-macrofago de caprinos naturalmente infectados pelo virus da artrite encefalite. Pesquisa Veterinaria Brasileira 32(12):1225-1229. Departamento de Clinica Medica, Faculdade de Medicina Veterinaria e Zootecnia, Universidade de Sao Paulo, Avenida Prof. Dr. Orlando Marques de Paiva 87, Cidade Universitaria, Sao Paulo, SP 05508-270, Brazil. E-mail: camilafb@usp.br Caprine arthritis encephalitis (CAE) and caseous lymphadenitis (CL) have high incidence and transmissibility in small ruminants. Since both virus have tropism for macrophages and monocytes and affect the innate immune response, it is believed that CAE can predispose the animal to infection by Corynebacteruim pseudotuberculosis, the etiological agent of CL. To confirm this hypothesis, we evaluated phagocytosis from the monocyte-macrophage cells from 30 Saanen goats. Goats were uniformly divided in two groups according to results of agar gel immunodiffusion test for CAE virus (CAEV). Peripheral blood mononuclear cells were isolated by density gradient centrifugation and the monocyte-macrophage cells were isolated from the mononuclear cells by their adhesion properties in plaques. Afterwards, phagocytosis of C. psudotuberculosis was performed for two hours at 37 degrees C, 5% of CO2, and assessed by microscopic visualization. There was no difference in the percentage of monocyte-macrophage cells that phagocytozed C. bovis between groups (P = 0.41). However, when phagocytosis rates were classified according to the number of C. pseudotuberculosis phagocyted, the percentage of monocyte-macrophage cells that internalized more than 12 bacteria were higher in serologically CAEV positive animals compared to the serologically negative ones (P < 0.001). Furthermore, a positive and significant correlation (r = 0.488; P = 0.006) between the percentage of monocyte-macrophage cells that internalized more than 12 bacteria and the percentage of monocyte that were carrying out phagocytosis was also encountered in serologically CAEV positive goats, however the same were not observed in serologically negative ones. These results demonstrated an alteration in the intensity of C. pseudotuberculosis phagocytosis by monocytes-macrophages from goats infected by CAEV. Thus, these results indicated that goats infected with CAEV may be more susceptible to CL.

Infection and immune-mediated meningococcal-associated arthritis: combination features in the same patient

We present a case of a 16-year-old male patient with sudden-onset, rash, arthritis and meningitis by Neisseria meningitidis one week after an acute upper respiratory infection. On the 10th day of treatment followed by neurological and arthritis clinical improvement, he presented once again a tender and swollen left knee with a moderate effusion, and active and passive range of motion was severely limited secondary to pain, and when he was submitted to surgical drainage and synovial fluid analysis he showed inflammatory characteristics. A non-steroidal anti-inflammatory drug was taken for five days with complete improvement of symptoms. The case is notable for its combination of features of septic and immune-mediated arthritis, which has rarely been reported in the same patient.