259 resultados para Alignments.
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Pós-graduação em Agronomia (Genética e Melhoramento de Plantas) - FCAV
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Pós-graduação em Geociências e Meio Ambiente - IGCE
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Coffee is one of the main products of Brazilian agriculture, the country is currently the largest producer and exporter. Knowing the growth pattern of a fruit can assist in the development of culture indicating for example, the times of increased fruit weight and its optimum harvest, essential to improve the management and quality of coffee. Some authors indicate that the growth curve of the coffee fruit has a double sigmoid shape. However, it consists of just a visual observation without exploring the use of regression models. The aims of this study were: i) determine if the growth pattern of the coffee fruit is really double sigmoidal; ii) to propose a new approach in weighted importance re-sampling to estimate the parameters of regression models and select the most suitable double sigmoidal model to describe the growth of coffee fruits; iii) to study the spatial distribution effect of the crop in the growth curve of coffee fruits. In the first article the aim was determine if the growth pattern of the coffee fruit is really double sigmoidal. The models double Gompertz and double Logistic showed significantly superior fit to models of simple sigmoid confirming that the standard of coffee fruits growth is really double sigmoidal. In the second article we propose to consider an approximation of the likelihood as the candidate distribution of the weighted importance resampling, aiming to facilitate the process of obtaining samples of marginal distributions of each parameter. This technique was effective since it provided parameters with practical interpretation and low computational effort, therefore, it can be used to estimate parameters of double sigmoidal growth curves. The nonlinear model double Logistic was the most appropriate to describe the growth curve of coffee fruits. In the third article aimed to verify the influence of different planting alignments and sun exposure faces in the fruits growth curve. A difference between the growth rates in the two stages of fruit development was identified, regardless the side. Although it has been proven differences in productivity and quality of coffee, there was no difference between the growth curves in the different planting alignments herein studied.
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Pós-graduação em Agronomia (Energia na Agricultura) - FCA
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Pós-graduação em Geologia Regional - IGCE
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
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Pós-graduação em Genética e Melhoramento Animal - FCAV
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Caseins are the major milk proteins associated with lactation performance, milk composition and cheese yield efficiency, representing around 80% of the total amount of proteins found in milk. Among the caseins, kappa-casein is the protein that stabilizes micelle structure during milk coagulation process and being used during the cheese production. The kappa-casein gene (CSN3) has been previously mapped to buffalo chromosome 7 using a radiation hybrid panel and a comparative map was established using the sequence from bovine chromosome 6. The molecular structure of this gene has also been established in river buffalo, with a total length of 13,191 bp (GenBank: AM900443.1) and containing five exons. In this study we searched for single nucleotide variations in specific regions of the CSN3 gene in three animals representing the Murrah breed. Sequencing reactions were performed using ABI3730xl sequencer. The primer walking method was used to span the 5'-UTR and intron 2 regions of the gene, for which ten primer pairs were designed using Oligo 6 software. BLAST tool was used to verify the primers specificity. DNA sequences assemblies from all three animals were performed with Sequencher (R) software 4.1, while multiple alignments were performed using Clustal W software to identify single nucleotide variations. The sequencing revealed a total of 19 single nucleotide variations with 13 located in the upstream regulatory region of the gene (5'-UTR) and six on intron 2. These variations can be validated using commercial populations segregating specific economic traits.
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
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Sao Paulo State Research Foundation-FAPESP