Isolating and recognizing crudeoiklegrading [sic] bacteria of Queshm Island shores for biodegradation of crude oil pollutions

This research was carried out for recognizing Natural Flora Bacteria of oil pollution in the coasts of Queshm island. In The First steps, The coasts of this Island were scrutinized as a Field of research and For knowing whether oil stains exist or not. It gets obvious That southern coasts of Queshm have got oil pollution which is created by oil tankers which carry oil of Iran continental shelf. Them oil stains were sampled from to certain stations. In The First step, primary isolation of exisiting bacteria in every oil sample was done and then purification of each bacterium was carried out. Then each purified bacterium that has got strong, recognized, typic growth was enriched oil sample of T5 station. And Bacterium C4 (gram—negative coccobacillus) was chosen as the second priority From oil sample of TA station and Bacterium B1 (gram—positive coccus) was chosen as The third priority From oil sample of TI station. All The above mentioned bacteria were biochemically, physiologically and morphologically experimented For specking The species. According To The tests done and comparing with The tests done and comparing with the reference Berge y' s, bacterium A5 Pelongs to the species pseudomonas sp and becterium C4 belongs to the species Aeromonas sp and bacterium BI belongs to The species micrococcus sp. In The Last stage, bacterium with The First priority (TA5 pseudomonas sp) was used in the planned microcosm. The sake of optimum and adapting to Laboratory conditions Each enriched and purified bacterium was given a code for station and a code For itself . Then This bacterium was studied and it was proved that it has potentiality For using oil as a source of carbon. From oil samples of 10 stations, 30 various Colonies of bacterium were Isolated, of which 20 bacteria had the highest potentiality of growth. And the other bacteria that has no typic growth were omitted From being studied. Since all of These 20 bacterium are able to use oil, a bacterium with maximum rate of growth in the presence of crude oil and Lack of other hydrocarbonic sources and with The code A5 ( gram — negative Bacillus ) was chosen as First priority From The mentioned microcosm contains sea water , suspension oil degrading bacterium , crude oil, azote and various concentrations of carbon and Incubated in 30°` and shook 150 PRA1 According to the results , index oil degrading bacterium (pseudomonas sp) belongs oil sample of T5 stations (east of sheeb draz Gulf) which growth best and have the potentiality of degrading oil in 25 glli malas and 50 glli cheese water and with 5 gill urea .

一种中草药复方制剂对罗非鱼非特异免疫力的影响

将杜仲、当归、黄芪、枸杞等10味中草药均匀粉碎后配制成复方制剂,按质量分数为0(对照组)、1%(A组)、3%(B组)、6%(C组)添加到饲料中投喂罗非鱼Oreochromis niloticus,分别在饲养试验的第4、11、18、25、32天采样,检测其血清溶菌酶活性、头肾巨噬细胞吞噬活性、血清杀菌活性、红细胞比容等免疫指标的变化,并在最后一次采样中检测其脾脏脏器系数。用嗜水气单胞菌Aeromonas hydrophila对试验鱼进行人工感染试验,分析中草药对鱼体免疫功能及免疫保护率的增强作用。饲养结果表

高铁酸钾对几种常见鱼类病原菌的杀菌效果测定

试验测定了高铁酸钾(K2FeO4)溶液对8种常见鱼类病原菌的MIC(最小抑菌浓度,minimal inhibitory concen-tration,MIC)和MBC(最低杀菌浓度,minimal bactericidal concentration,MBC),并研究了不同浓度的K2FeO4溶液对几种病原菌的杀灭效果。结果显示,K2FeO4对温和气单胞菌(Aeromonas sobria)、河弧菌(Vibrio flurialis)、弧菌I组淡水亚组弧菌(Vibriogroup I freshwater

噬菌蛭弧菌的分离及初步鉴定

采用双层平板法,以滤膜过滤的方法来收集噬菌蛭弧菌,以嗜水气单胞菌(Aeromonas hudrophila)、荧光假单胞菌(Pseudomonas fluorescent)和绿脓杆菌(Pseudomonas aeruginosa)为宿主菌,进行噬菌蛭弧菌的分离研究;并在此基础上,通过接触酶检测和寄生性确认对噬菌蛭弧菌(Bdellovibrio bacteriovorus)进行了初步的鉴定。结果表明未使用滤膜过滤,采用自来水琼脂双层平板法分离噬菌蛭弧菌的效果较好;并经过接触酶和寄生性检测初步鉴定此BD-SP

欧洲鳗鲡免疫球蛋白阳性细胞的组织化学定位与分布特点

采用蛋白A亲和层析法分离纯化欧洲鳗鲡(Anguilla anguilla,欧鳗)血清免疫球蛋白(Ig),制备其特异性兔抗血清,并运用免疫组织化学技术研究了嗜水气单胞菌(Aeromonas hydrophila)感染的试验组鳗鲡和未经感染的对照组鳗鲡脾脏、肾脏和肝脏Ig阳性(Ig+)细胞定位与分布特点。结果表明:纯化后的Ig经SDS-PAGE检测含有分子质量约68 ku重链和26 ku轻链的2条清晰蛋白条带,由其制备的兔抗Ig血清效价达1∶51 200。对照组欧鳗脾脏Ig+细胞数较少,肾脏相对较多,肝脏未发

嗜水气单胞菌感染的中华鳖主要器官差减cDNA文库的构建

以致病性嗜水气单胞菌(Aeromonas hydrophila)人工感染的中华鳖(Trionyx sinensis)肝、脾、肾组织为材料,应用抑制性差减杂交(SSH)技术,构建了嗜水气单胞菌感染组织的差减cDNA文库。以中华鳖管家基因-βactin作为差减指标检测该文库差减效率达210倍,表明感染细菌后某些差异表达基因得到了相应倍数的富集。将获得的cDNA片段连接到pMD18-T载体并转化大肠杆菌DH5α感受态细胞。PCR阳性检测显示差减片段在150—800bp之间。该差减cDNA文库的构建为快速分离和鉴

广东中山地区气单胞菌环境株的分离、鉴定及系统发生关系

从广东省中山市的池塘水样、底泥、健康鱼、肠道及稻田土样中用Aeromonas的选择培养基分离到10株气单胞菌。通过生理生化测试、16S rDNA序列测定、与气单胞菌典型菌株的16S rDNA序列进行比对和聚类分析,对它们进行了鉴定,并研究了它们之间的系统发生关系。结果显示该地区环境中气单胞菌的优势种除A.hydrophila(HG1组)外,还有A.caviae(HG4组)、A.jandaei(HG9组)和A.veronii(HG10组),其中后两种是国内新记录。这是国内首次对环境中气单胞菌多样性进行研究。

嗜水气单胞菌和河弧菌二联疫苗对鲫的免疫效果

在采用正交试验设计优化了 Aeromonas hydrophila和 Vibrio fluvialis二联全菌疫苗制备工艺的基础上 ,研究了采用该工艺所制备的疫苗对鲫的免疫保护效果。结果表明注射免疫鲫4周后 ,抗 A.hydrophila和 V.fluvialis血清凝集效价分别为 :1∶ 4— 1∶ 3 2和 1∶ 2— 1∶ 1 6 ,平均都为 1∶ 1 0 ,但对攻毒均具有有效的保护作用。浸泡免疫、高渗浸泡免疫或加多糖浸泡免疫在免疫 4周后 ,对 A.hydrophila攻毒均无保护作用 ;然而 ,

鳖类疾病及其防治对策(七)

<正> (2)病原体:根据蔡完其等(1995)的研究,上海病鳖的病原菌是嗜水气单胞菌、普通变形菌(Proteus vulgaris)。然而齐彩霞等(1995)在湖北省分离、鉴定的病原菌是气单胞菌(Aeromonas sp.)、产碱菌(Alcaligemes sp.)。因此穿孔病是多种病原菌的鳖病。(3)危害与流行情况:从稚鳖到亲鳖都会患病,

鳖病防治技术问答(中)

<正> 9.问:红脖子病和大脖子病是不是一种病? 答:红脖子病和大脖子病是一种病。病鳖背甲失去光泽而呈暗黑色,颈部肿大,行动迟缓,鳖体消瘦。随着病情的日趋严重,颈部充血发红,成为红脖子现象,以致颈部不能正常伸缩。严重时,病鳖眼睛失明,口鼻、舌头都出血。解剖病鳖,见肝、脾脏均肿大,质脆易碎,口腔、食道、胃、肠的粘膜层呈明显的点状、弥蔓性出血占80％。从红脖子病鳖分离到病原菌是嗜水气单胞菌嗜水亚种(Aeromonas,hydrophila sub-sp.hydrophila);从大脖子病鳖分离到的也是嗜水气单

淡水养殖鱼类暴发性传染病致病细菌的研究

于1990—1991年的5—10月间,从湖北、湖南、河南和广东4省的9种淡水养殖鱼类的发病鱼睥、肝、肾中分离的93株细菌为材料,经100多个表型特征和细菌DNA中G+Cmol％遗传型特性测定等研究结果,可归属为产气单胞菌属中的运动性嗜中温产气单胞菌和弧菌属中的河弧菌。按照国际细菌命名法规优先律应为点状产气单胞菌[Aeromonas punctata(Zimmermann)nom.rev];而河弧菌与以前所描述的生物变种Ⅰ、Ⅱ型不同:在43℃能生长;发酵水杨苷产酸;利用D-葡糖酸钙、腐胺;不能利用戊二酸,D

鲢鳙鱼流行性传染病的流行病学和细菌病因的初步研究

1990年3～10月,作者对湖北、湖南,河南三省部分地区的28个养殖场进行了鲢鳙鱼流行病学调查和致病菌的初步研究。结果显示:在调查范围内发病率达60%以上;受感染的除鲢、鳙外,还有鲫、鳊(鲂)、鲤、草鱼以及其它野杂鱼、河蚌;分离到138株细菌,经鉴定可归为三类,早期(3～4月)出现的鲁克氏耶尔森菌(Yersinia ruckeri)、中后期(5月下旬以后)出现的产气单胞菌(Aeromonas sp.)及弧菌(Vibrio sp.)。结合水质环境条件分析,致病细菌是继发侵入者,原发病因除水体的理化因子外,不

鳗鲡烂尾病病原菌的研究

鳗鲡烂尾病是鳗鲡养殖中的一种常见病,主要流行于夏季。在日本报道了由枉状屈桡杆菌引起的鳗鲡烂尾病。本文记述了用TYE培养基从广东潮安养鳗场病鳗中分离到的另外一种引起烂尾病的病原菌,并研究了该病原菌的致病作用,生物学和生理生化特性,鉴定为点状产气单胞杆菌(Aeromonas punctata)。该菌对青霉素、新生素、磺胺噻唑不敏感,而对呋唑唑酮、土霉素、氯霉素、合霉素和金霉素等敏感。

塔式生物滤池处理洗涤剂工业废水的模拟试验

本项研究工作表明,塔式生物滤池在处理模拟洗涤剂工业废水时,能够适应和克服一般好氧生化法所不能解决的泡沫问题,并对废水中的LAS和COD具有一定的去除效果。根据实验结果,初步认为塔滤可应用于洗涤剂工业废水的生化处理,并向洗涤剂行业首次推荐这种废水生物学净化方法。从塔滤的生物膜中分离出了优势菌,经鉴定为一种气单胞菌(Aeromonas sp.D-4)。

二龄草鱼肠炎病发病机理

本文通过对患肠炎病的二龄草鱼病鱼和正常草鱼的肠道及血液中的产气单胞菌(Aeromonas)的数量和毒力的比较,病鱼肠道、血液和其他内脏中的产气单胞菌所出现比率的调查,不同水温对该菌致病力的影响和用菌体提取液接种鱼体,以及用鲎试剂(鲎变形细胞溶解物Tachypleus Amebocyte Lysatc “TAL”)对病鱼和正常鱼体内的内毒素进行测定等一系列的试验和观察,从而推断出二龄草鱼细菌性肠炎病的发病机理。