Effect of food on immature development, consumption rate, and relative growth rate of Toxorhynchites splendens (Diptera: Culicidae), a predator of container breeding mosquitoes

Food utilization by the larvae of Toxorhynchites splendens (Wiedemann) was studied in the laboratory by offering larvae of Aedes aegypti Linnaeus, Anopheles stephensi (Liston), and Culex quinquefasciatus (Say). Quantitative analyses of data indicated that immature development was significantly faster with increase in food availability. The regression analysis showed that the degrees of the relationship between immature duration (Id) and food availability were higher when offered early instars of prey (first and second instars) than late instars. Consumption rate (Cr) of the predator increased with increase in food availability and this relationship was highly significant when larvae of An. stephensi were offered as food. Consumption rate to food level decreased with increase in the age class of the prey. There was a significant negative correlation between Id and Cr. This aspect helps to increase population turnover of T. splendens in a shorter period when the prey is abundant. Conversely, the predator compensated the loss in daily food intake at low food level by extending Id thereby attains the minimum threshold pupal weight for adult emergence. There was an increase in the relative growth rate (RGR) of the predator when An. stephensi was offered as prey and this was related to the high protein content of the prey per body weight. There was a positive correlation between Cr and RGR. This adaptive life characteristic strategy of this predator is useful for mass-rearing for large scale field release programmes in the control of container breeding mosquitoes is discussed.

Physiological responses of two tropical weeds to shade: I. Growth and biomass allocation

Ipomoea asarifolia (Desr.) Roem. & Schultz (Convolvulaceae) and Stachytarpheta cayennensis (Rich) Vahl. (Verbenaceae), two weeds found in pastures and crop areas in Brazilian Amazonia, were grown in controlled environment cabinets under high (800-1000 µmol m-² s-¹) and low (200-350 µmol m-² s-¹) light regimes during a 40-day period. For both species leaf dry mass and leaf area per total plant dry mass, and leaf area per leaf dry mass were higher for low-light plants, whereas root mass per total plant dry mass was higher for high-light plants. High-light S. cayennensis allocated significantly more biomass to reproductive tissue than low-light plants, suggesting a probably lower ability of this species to maintain itself under shaded conditions. Relative growth rate (RGR) in I. asarifolia was initially higher for high-light grown plants and after 20 days started decreasing, becoming similar to low-light plants at the last two harvests (at 30 and 40 days). In S. cayennensis, RGR was also higher for high-light plants; however, this trend was not significant at the first and last harvest dates (10 and 40 days). These results are discussed in relation to their ecological and weed management implications.

Copper oxychloride fungicide and its effect on growth and oxidative stress of potato plants

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Influence of fertilization on the growth of radicchio "Rosso di Chioggia" cultivated in two different environments

Este trabajo evalúa la influencia de la fertilización en radicchio tipo "Rosso de Chioggia" (precocidad media) sobre algunos índices de crecimiento. Se realizaron ensayos durante dos campañas en Rovigo (Italia) y una en Mendoza (Argentina), aplicándose dosis crecientes de NPK, identificadas como N0P0K0, N1P1K1, N2P2K2, en Mendoza y en Rovigo, además, N3P2K2. Durante el cultivo se calcularon índices de crecimiento como: relative growth rate (RGR), net assimilation rate (NAR), leaf area ratio (LAR), specific leaf area (SLA), leaf weigh ratio (LWR), crop growth ratio (CGR), leaf area index (LAI) and leaf area duration (LAD). En Mendoza, el CGR estuvo fuertemente influenciado por NAR desde el trasplante hasta alcanzar 776 grados días (GDD); desde 1052 a 1653 GDD el CGR fue afectado por el LAI el cual aumentó marcadamente debido a las condiciones ambientales favorables. Entre los 1052 y 1653 GDD el incremento del LAI determinó una reducción en la eficiencia fotosintética. En Rovigo, la tendencia de los índices fue disímil en los dos años, encontrándose respuestas diferentes en LAR y en SLA. En el segundo año, el CGR siempre arrojó valores más altos, mientras que NAR no difirió en ninguno de los años. En la segunda mitad del ciclo, CGR estuvo fuertemente asociado a una menor eficiencia fotosintética, debido a la formación de la cabeza. Valores elevados de LAI indicaron una extensión del ciclo, retrasando la formación de la cabeza. Las plantas alcanzaron la madurez comercial con LWR entre 0,35 - 0,40 g g-1. En ambos ambientes, no se observó claramente el efecto de la fertilización sobre los índices; si bien las dosis más altas mostraron mayor actividad de crecimiento en las etapas tempranas.

Growth response to ozone of annual species from Mediterranean pastures

Ozone (O3) phytotoxicity has been reported on a wide range of plant species. However, scarce information has been provided regarding the sensitivity of semi-natural grassland species, especially those from dehesa Mediterranean grasslands, in spite of their great biological diversity and the high O3 levels recorded in the region. A screening study was carried out in open-top chambers (OTCs) to assess the O3-sensitivity of representative therophytes of these ecosystems based on the response of selected growth-related parameters. Three O3 treatments and 3 OTCs per treatment were used. Legume species were very sensitive to O3, because 78% of the tested species showed detrimental effects on their total biomass relative growth rate (RGR) following their exposure to O3. The Trifolium genus was particularly sensitive showing O3-induced adverse effects on most of the assessed parameters. Gramineae plants were less sensitive than Leguminosae species because detrimental effects on total biomass RGR were only observed in 14% of the assessed species. No relationship was found between relative growth rates when growing in clean air and O3 susceptibility. The implications of these effects on the performance of dehesa acidic grasslands and on the definition of ozone critical levels for the protection of semi-natural vegetation are discussed.

Lobe connections and lobe crowding are associated with growth rate in the lichen Xanthoparmelia conspersa

The association between lobe connections and the degree of lobe crowding and radial growth was studied in thalli of the foliose lichen Xanthoparmelia conspersa. In 35 thalli, 15% of the lobes were not physically connected to either of their neighbours before the lobes merged into the centre of the thallus. Twenty-five percent of the lobes were connected in pairs and 29% in groups of three. Approximately 5% of the lobes were interconnected in larger groups of six or more. The mean number of lobes per group in a thallus was positively correlated with thallus diameter and with the degree of lobe growth variation but was unrelated to annual radial growth rate (RGR). The degree of crowding of the lobes in a thallus was defined as a 'crowding index', viz., the product of lobe density and mean lobe width. Crowding index increased rapidly with size in smaller thalli but changed less with size in larger thalli. Crowding index was positively correlated with RGR but was unrelated to lobe growth variation. Lobes removed from large thalli and glued in various configurations to simulate different degrees of crowding did not demonstrate an association between lobe crowding and RGR over one year. These results suggest that the pattern of lobe connectivity of a thallus is associated with lobe growth variation in X. conspersa. The degree of lobe crowding is associated with the increase in RGR with thallus size in smaller thalli and by restricting lobe width, could also be a factor associated with the more constant growth of larger thalli.

Growth and gas exchange responses of Brazilian pepper (Schinus terebinthifolius) and native South Florida species to salinity

Schinus terebinthifolius Raddi (Schinus) is an invasive exotic species widely found in disturbed and native communities of Florida. This species has been shown to displace native species as well as alter community structure and function. The purpose of this study was to determine if the growth and gas exchange patterns of Schinus, under differing salinity conditions, were different from native species. Two native upland glycophytic species (Rapanea punctata and Randia aculeata) and two native mangrove species (Rhizophora mangle and Laguncularia racemosa) were compared with the exotic. Overall, the exotics morphologic changes and gas exchange patterns were most similar to R. mangle. Across treatments, increasing salinity decreased relative growth rate (RGR), leaf area ratio (LAR) and specific leaf area (SLA) but did not affect root/shoot ratios (R:S). Allocation patterns were however significantly different among species. The largest proportion of Schinus biomass was allocated to stems (47%), resulting in plants that were generally taller than the other species. Schinus also had the highest SLA and largest total leaf area of all species. This meant that the exotic, which was taller and had thinner leaves, was potentially able to maintain photosynthetic area comparable to native species. Schinus response patterns show that this exotic exhibits some physiological tolerance for saline conditions. Coupled with its biomass allocation patterns (more stem biomass and large area of thin leaves), the growth traits of this exotic potentially provide this species an advantage over native plants in terms of light acquisition in a brackish forested ecosystem.

Estudo comparativo de dois tipos de cultivo: monocultivo (camarões) versus cultivo integrado (algas/camarões)

The integrated culture of seaweed and aquatic animals is an ancient practice in Asian countries. The expansion of this practice to western countries is consequence of the recognition of this system as a sustainable alternative that allows economical diversification and mitigation of environmental impacts generated by effluents of aquaculture. This study evaluated the growth of the seaweed Gracilaria caudata and of the shrimp Litopenaeus vannamei in monoculture (shrimps) and integrated culture (shrimps and algae) systems, and accessed the effect of the seaweed in the water quality. There were two treatments in the experiment: monoculture (shrimps) and integrated culture (shrimps/ algae). The organisms were cultured in 6 aquaria (10L) filled with seawater (35.0±0.0 PSU and 28.1±0.4°C) for 28 days. The nutrients of water (PO43-, NH4+, NO2-, NO3- and DIN), the biomass and the relative growth rate (RGR, % day-1) of seaweed and shrimps were measured weekly. The parameters pH, temperature, salinity and dissolved oxygen were measured daily. The concentration of NH4+ in integrated culture (62.8±25.2µM) was lower (Mann-Whitney p<0.001) than in monoculture (85.6±24.3µM). The mean of PO4- in monoculture (10.4±4.6µM) was markedly higher (Mann-Whitney; p=0.024) than that in integrated culture (8.7±4.1µM). The level of dissolved oxygen in integrated culture (6.0±0.6mg/L) was higher (t-Student; P=0.014) than that in shrimp monoculture (5.8±0.6mg/L). The mean values of the parameters pH, NO2-, NO3- and DIN were 7.5±0.2, 10.1±12.2µM, 24.5±3.2µM and 120.17±30.76µM in monoculture, and 7.5±0.2, 10.5±13.2µM, 27.4±3.5µM and 100.76±49.59µM in integrated culture. There were not differences in these parameters between treatments. The biomass and RGR of seaweed reached 15.0±1.9g and 7.4±2.8% day-1 at the end of the experiment. The performance of shrimp was favorable in monoculture (1.5±0.8g; 5.7±1.6% dia-1) and in integrated culture (1.5±0.7g; 5.2±1.2% dia-1), and the rate of survival was 100% in both treatments. The tolerance and favorable performance of Gracilaria caudata suggest that this seaweed might be integrated into shrimp (Litopenaeus vannamei) culture systems

Curva de crescimento e acúmulo de matéria seca em couve-flor cultivada em substrato

Aiming to evaluate the curve of growth and dry matter accumulation in cauliflower cv. Verona in commercial substrate for vegetables Plantmax (R), an experiment was conducted in a greenhouse at UNESP, Jaboticabal, SP. The experimental design was randomized blocks with six treatments, five replicates, each plot had a plan. The treatments consisted of sampling times (20, 30, 40, 50, 60 and 70 days after transplantation). Evaluations of plant height, leaf number, stem diameter, leaf area, dry matter of shoot, root and whole plant, and fresh weight of the inflorescence. Furthermore, calculated the relative growth rate (RGR) of leaves, stems, roots, flowers and whole plant, presented in g day(-1) plant(-1). The hybrid of cauliflower 'Verona' shows initial slow growth, stepping up from 30 days after transplantation. The maximum accumulation of dry matter per plant was 87g at 70 DAT, with the participation of sheets of 53.8%, stem 9.9%, 30.7% of inflorescence and root 6.5%. The cultivation cauliflower Verona in substrate showed high accumulation of dry Inflorescence.

Fotossíntese, reservas orgânicas e rebrota do capim-mombaça (Panicum maximum Jacq.) sob diferentes intensidades de desfolha do perfilho principal

Buscando-se avaliar morfofisiologicamente a rebrota do capim-mombaça, quatro desfolhas foram impostas ao perfilho principal, sendo estudado o comportamento da planta em termos da taxa de expansão da área foliar, crescimento do sistema radicular, nível de carboidratos totais não estruturais (CTNE) da raiz e do colmo, taxa de crescimento relativo (TCR), taxa de assimilação líquida (TAL) e razão de área foliar (RAF) às idades de 2, 5, 9 e 16 dias após as desfolhas, bem como da taxa fotossintética máxima às idades de 2, 6 e 13 dias das folhas remanescentes à desfolha. As desfolhas foram as seguintes: remoção de todas as lâminas foliares (desfolha total), a remoção da lâmina da folha adulta mais jovem (desfolha superior), a remoção das lâminas das duas folhas adultas mais velhas (desfolha inferior) e controle (sem desfolha), juntamente com o corte dos demais perfilhos, realizado a 8 cm do solo. Foram observadas cinco repetições por tratamento, segundo o delineamento inteiramente casualizado. As folhas adultas não diferiram quanto às taxas fotossintéticas máximas, que exibiram aumento nos primeiros dias após a desfolha, e queda aos 13 dias. A desfolha reduziu os teores de CTNE da base do colmo, principalmente nas plantas sob desfolha total. Comprometimento do crescimento do sistema radicular e do teor de CTNE das raízes foi observado nas plantas sob desfolha total, que também tiveram sua TCR reduzida nos primeiros dias de rebrotação. Entretanto, o aumento na RAF possibilitou a estas plantas recuperação da TCR e alta taxa de expansão da área foliar, igualando a área foliar das demais plantas aos 16 dias de rebrota.

Translocação orgânica, produtividade e rendimento de óleo essencial de Mentha piperita L. cultivada em solução nutritiva com variação dos níveis de N, P, K e Mg

O estudo objetivou avaliar translocação orgânica, índices fisiológicos da análise de crescimento e rendimento do óleo essencial de Mentha piperita L. cultivada em solução nutritiva com variação dos níveis de N, P, K e Mg. Assim, foram avaliados os quatro tratamentos contendo 50% N, P, K, 25% Mg; 50% N, P, K, Mg; 65%N, 50%P, 25%K, 100% Mg e 100% N, P, K, Mg. A translocação orgânica foi avaliada por meio da determinação da razão de massa foliar (RMF) e da distribuição de massa seca para os diferentes órgãos. Os índices fisiológicos razão de área foliar (RAF), área foliar específica (AFE), taxa assimilatória líquida (TAL) e taxa de crescimento relativo (TCR) derivadas que compõem a análise de crescimento foram estimados pelo programa ANACRES, após ajuste exponencial quadrático da área foliar e massa seca de lâminas foliares e total da planta em relação ao tempo. O rendimento do óleo essencial, em porcentagem, foi calculado após extração da parte aérea por hidrodestilação. As plantas submetidas ao tratamento com nível completo de nutrientes (100%N/P/K/Mg) exportaram com menor eficiência o material orgânico a partir da folha e a RMF mostrou queda mais lenta, devido à retenção desse material por mais tempo no local de sua produção. Além disso, não apresentaram melhor produtividade e as curvas da TAL e TCR mostraram quedas mais lentas. As plantas submetidas ao tratamento com 65%N/50%P/25%K/100%Mg revelaram adequada exportação de matéria orgânica da folha para caule e pecíolos, conforme demonstra a RMF e a distribuição de massa seca para esses órgãos. Revelaram ainda a RAF mais elevada no inicio do desenvolvimento e mais baixa aos 94 DAT, indicando sombreamento das folhas como resultado de seu crescimento. A AFE dessas plantas mostrou menor variação durante o ciclo, refletindo espessura mais constante de suas folhas. A TAL e a TCR apresentaram curvas decrescentes, com quedas bem acentuadas em relação às demais plantas. Todos esses índices indicam melhor produtividade das plantas submetidas ao tratamento 65%N/50%P/25%K/100%Mg, que também apresentaram rendimento de óleo essencial, em média, maior durante o ciclo. Conclui-se que a redução de 35% de N, 50% de P, 75% de K (65%N/50%P/25%K/100%Mg) é indicada para o cultivo e extração de óleo essencial de M. piperita.

Índices biométricos e fisiológicos em feijoeiro sob diferentes doses de adubação fosfatada

The study had the objective of evaluate the growth of bean cultivar Carioca early cultivated during the rainy season under different doses of phosphorus applied to soil. We adopted the experimental design at random blocks, evaluating six doses of phosphorus (0, 30, 60, 90, 120 and 150 kg P(2)O(5) ha(-1)), relying in the form of superphosphate triple, with five replicates. Biometric indices were evaluated leaf area (cm(2) plant(-1)) and dry matter (g seedling(-1)). Physiological indices of crop growth rate (CGR, g m(-2) day(-1)), relative growth rate (RGR, g g(-1) day(-1)), net assimilation rate (NAR, g m(-2) day(-1)), leaf area ratio (LAR, cm(2) g(-1)) and specific leaf area (SLA, cm(2) g(-1)) were obtained through analysis of functional growth. The data from the biometric indices were submitted to analysis of variance, with subsequent construction of regression models. The dry matter and leaf area of bean Carioca Early increase linearly with increasing doses of applied phosphorus in the soil. The dry matter has increased constantly throughout the cycle, and the leaf area indices reach its maximum at 52 days after emergence (DAE). The restriction of phosphorus reduces the Relative Growth Rate at the beginning of the development and lengthen the physiological cycle of bean cultivar Carioca early.

Influência de diferentes níveis de cálcio em plantas de estilosantes (Stylosanthes guyanensis (Aubl.) Sw. cv. Cook), avaliados através de alguns parâmetros fisiológicos

O objetivo do presente trabalho foi estudar os efeitos de diferentes níveis de cálcio, no desenvolvimento de plantas de Stylosanthes guyanensis (Aubl.) Sw. cv. Cook, através de alguns parâmetros que compõem a análise fisiológica de crescimento. Para tal foram empregados 4 tratamentos a saber: T1 (200 mg de cálcio/litro); T2 (133,33 mg de cálcio/litro); T3 (66,66 mg de cálcio/litro) e T4 (omisso em cálcio). O trabalho foi montado em cultivo hidropônico, utilizando-se a solução nutritiva n° 1 de Hoagland & Arnon e conduzido em casa de vegetação. Foram realizadas observações em intervalos entre 5 coletas, distribuidas da seguinte maneira: intervalo I (entre 24° e 38° dia pós-germinação; intervalo II ( do 38° ao 52° dia); intervalo III (do 52° ao 66° dia) e intervalo IV (do 66° ao 80° dia). Os parâmetros estudados foram: a. taxa assimilatória líquida (TAL); b. taxa de crescimento relativo (TCR) e c. razão alfa. Dos resultados obtidos pode-se concluir que soluções com 200 e 133,33 mg de cálcio/litro, foram mais eficientes em promover o desenvolvimento de plantas de estilosantes.

Crescimento de plantas de Salvia officinalis sob ação de reguladores de crescimento vegetal

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)