The nitrogen sufficiency index underlying estimates of nitrogen fertilization requirements of common bean

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

The nitrogen sufficiency index underlying estimates of nitrogen fertilization requirements of common bean

Chlorophyll determination with a portable chlorophyll meter can indicate the period of highest N demand of plants and whether sidedressing is required or not. In this sense, defining the optimal timing of N application to common bean is fundamental to increase N use efficiency, increase yields and reduce the cost of fertilization. The objectives of this study were to evaluate the efficiency of N sufficiency index (NSI) calculated based on the relative chlorophyll index (RCI) in leaves, measured with a portable chlorophyll meter, as an indicator of time of N sidedressing fertilization and to verify which NSI (90 and 95 %) value is the most appropriate to indicate the moment of N fertilization of common bean cultivar Perola. The experiment was carried out in the rainy and dry growing seasons of the agricultural year 2009/10 on a dystroferric Red Nitosol, in Botucatu, São Paulo State, Brazil. The experiment was arranged in a randomized complete block design with five treatments, consisting of N managements (M1: 200 kg ha-1 N (40 kg at sowing + 80 kg 15 days after emergence (DAE) + 80 kg 30 DAE); M2: 100 kg ha-1 N (20 kg at sowing + 40 kg 15 DAE + 40 kg 30 DAE); M3: 20 kg ha-1 N at sowing + 30 kg ha-1 when chlorophyll meter readings indicated NSI < 95 %; M4: 20 kg ha-1 N at sowing + 30 kg ha-1 N when chlorophyll meter readings indicated NSI < 90 % and, M5: control (without N application)) and four replications. The variables RCI, aboveground dry matter, total leaf N concentration, production components, grain yield, relative yield, and N use efficiency were evaluated. The RCI correlated with leaf N concentrations. By monitoring the RCI with the chlorophyll meter, the period of N sidedressing of common bean could be defined, improving N use efficiency and avoiding unnecessary N supply to common bean. The NSI 90 % of the reference area was more efficient to define the moment of N sidedressing of common bean, to increase N use efficiency.

Re-evaluation of pulmonary titanium dioxide nanoparticle distribution using the "relative deposition index": Evidence for clearance through microvasculature

ABSTRACT: BACKGROUND: Translocation of nanoparticles (NP) from the pulmonary airways into other pulmonary compartments or the systemic circulation is controversially discussed in the literature. In a previous study it was shown that titanium dioxide (TiO2) NP were "distributed in four lung compartments (air-filled spaces, epithelium/endothelium, connective tissue, capillary lumen) in correlation with compartment size". It was concluded that particles can move freely between these tissue compartments. To analyze whether the distribution of TiO2 NP in the lungs is really random or shows a preferential targeting we applied a newly developed method for comparing NP distributions. METHODS: Rat lungs exposed to an aerosol containing TiO2 NP were prepared for light and electron microscopy at 1 h and at 24 h after exposure. Numbers of TiO2 NP associated with each compartment were counted using energy filtering transmission electron microscopy. Compartment size was estimated by unbiased stereology from systematically sampled light micrographs. Numbers of particles were related to compartment size using a relative deposition index and chi-squared analysis. RESULTS: Nanoparticle distribution within the four compartments was not random at 1 h or at 24 h after exposure. At 1 h the connective tissue was the preferential target of the particles. At 24 h the NP were preferentially located in the capillary lumen. CONCLUSION: We conclude that TiO2 NP do not move freely between pulmonary tissue compartments, although they can pass from one compartment to another with relative ease. The residence time of NP in each tissue compartment of the respiratory system depends on the compartment and the time after exposure. It is suggested that a small fraction of TiO2 NP are rapidly transported from the airway lumen to the connective tissue and subsequently released into the systemic circulation.

MERIS Terrestrial Chlorophyll Index

Resources created at the University of Southampton for the module Remote Sensing for Earth Observation

Resposta de plantas de alho livres de vírus ao nitrogênio em ambiente protegido

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Índice relativo de clorofila e o estado nutricional em nitrogênio durante o ciclo do cafeeiro fertirrigado

O clorofilômetro (SPAD-502, Minolta) é um aparelho portátil que permite obtenção do índice relativo de clorofila (IRC) de modo simples, rápido e não destrutivo no próprio campo, o qual se correlaciona com a concentração de N em várias culturas, e que pode tornar mais rápida a correção de deficiência, otimizando a utilização da fertirrigação. Objetivou-se com este trabalho verificar se o IRC pode ser indicativo do estado nutricional em N, podendo auxiliar no ajuste da fertirrigação nitrogenada durante o ciclo do cafeeiro. O experimento foi desenvolvido em Botucatu, SP, em área com cafeeiros do cultivar Catuaí Vermelho, com dois anos, no espaçamento de 2,5 x 0,8 m e irrigado por gotejamento. O delineamento experimental utilizado foi em blocos casualizados, com quatro repetições, aplicando-se um tratamento sem adubação, mas com irrigação, e mais quatro tratamentos utilizando fertirrigação com 33, 66, 100 e 133 % da dose de N recomendada, parcelada semanalmente (52,8; 105,6; 160,0; e 212,7 kg ha-1, respectivamente). O IRC aumentou linearmente com as doses de N, e a produtividade correlacionou-se significativamente com o IRC, do florescimento até a colheita, porém não com a concentração de N foliar. Os IRCs nos cafeeiros com maior produtividade foram de 81,5 a 83,2 (florescimento e início de expansão dos frutos), de 76,2 a 78,3 (expansão dos frutos), de 68,3 a 69,8 (início da granação), de 64,0 a 65,9 (na granação) e de 61,7 a 62,7 unidades SPAD (na maturação). O clorofilômetro pode ser utilizado para definir a probabilidade de resposta ao N no decorrer do ciclo do cafeeiro.

Nitrogênio em cobertura e molibdênio via foliar no feijoeiro irrigado cultivado em solo de cerrado

O nitrogênio é o nutriente exigido em maior quantidade pelo feijoeiro e, mesmo quando suprida adequadamente desse nutriente, a planta de feijão pode ter sua produtividade limitada pela deficiência de molibdênio, pela participação deste no metabolismo do nitrogênio. O objetivo deste trabalho foi avaliar a resposta do feijoeiro (Phaseolus vulgaris L.), cultivar 'BRS Pontal', irrigado, à aplicação de nitrogênio em cobertura e molibdênio via foliar, quando cultivado em Neossolo Quartzarênico, no município de Cassilândia, MS. O delineamento experimental foi o de blocos ao acaso, no esquema fatorial 4x2, com quatro repetições. Os tratamentos foram constituídos de quatro doses de nitrogênio (0, 30, 60 e 120 kg ha-1) aplicados em cobertura, utilizando como fonte a ureia, e pela aplicação ou não de molibdênio (80 g ha-1) via foliar, na forma de molibdato de amônio. O índice relativo de clorofila nas folhas do feijoeiro foi aumentado pela aplicação de nitrogênio em cobertura e molibdênio via foliar. A aplicação de nitrogênio em cobertura proporcionou aumento na produtividade de grãos do feijoeiro apenas quando combinado com o fornecimento de molibdênio via foliar. O fornecimento de 80 g ha-1 de molibdênio via foliar aumentou a eficiência de utilização do nitrogênio pelo feijoeiro.

Molibdênio via semente e nitrogênio em cobertura no feijoeiro irrigado em solo de cerrado

O nitrogênio é o nutriente mais exigido pelo feijoeiro e seu metabolismo pode ser seriamente prejudicado pela deficiência de molibdênio. Conduziu-se este trabalho, com o objetivo de avaliar o efeito da aplicação de molibdênio via semente e nitrogênio em cobertura nas características agronômicas e produtividade do feijoeiro 'BRS Pontal' irrigado, cultivado em Neossolo Quartzarênico, no município de Cassilândia, MS. O delineamento experimental utilizado foi o de blocos casualizados, no esquema fatorial 4x2, com quatro repetições. Os tratamentos foram constituídos pela aplicação ou não de Mo (80 g ha-1) via semente e quatro doses de N (0, 30, 60 e 120 kg ha-1), em cobertura aos 16 dias, após a emergência, na forma de uréia. A aplicação de Mo via semente aumentou o índice relativo de clorofila, o número de vagens por planta, a massa e a produtividade de grãos no feijoeiro, principalmente com o uso de altas doses de N em cobertura. O índice relativo de clorofila, o número de vagens por planta, a massa e a produtividade de grãos do feijoeiro foi aumentada linearmente pela aplicação de até 120 kg ha-1 de N em cobertura. O fornecimento de Mo via tratamento de semente aumentou a eficiência de utilização do N pelo feijoeiro.

Teores foliares de nutrientes, índice relativo de clorofila e teores de nitrato e de potássio na seiva do pecíolo na videira 'Niagara Rosada

A nutritional survey was carried out in 93 vineyards of the wine regions of Jundiai, Sao Miguel Arcanjo and Jales (state of São Paulo-Brazil), aiming to compare the nutrient concentration in leaf samples, the concentration of nitrate and potassium in the petiole sap and the relative chlorophyll index (IRC) of 'Niagara Rosada' vineyard. Leaves, blade and petiole in full bloom vines were sampled in 20 plants selected from each vineyard, and evaluations of the IRC in the leaf blade and the levels of NO3-and K in the petioles sap were realized. Macro and micronutrients concentrations were measured in leaf samples. The sampled vineyards were grouped by region of study, and each vineyard was considered as a replicate sample, totaling 45, 24 and 24 repetitions, in Jundiai, Sao Miguel Arcanjo and Jales, respectively. Sampled vineyards from the Jales region showed higher leaf concentrations of P, K, Ca, Mg, S, Fe and Mn, and also higher IRC and levels of nitrate and potassium in the petiole. These differences were related to the different rootstocks used, which were 'IAC 766', 'IAC 572' and 'Riparia of Traviu', respectively, for Jundiai, Jales and Sao Miguel Arcanjo. The differences highlight the need to adjust the concentration ranges of nutrients in leaf analysis, through regional trials, taking into account the rootstock and the region under study.

Uso do clorofilômetro portátil na determinação da adubação nitrogenada de cobertura em cultivares de feijoeiro

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Resposta de cultivares de feijão comum à adubação nitrogenada

Pós-graduação em Agronomia (Agricultura) - FCA

Estimativa da necessidade de nitrogênio em cobertura no feijoeiro IAC Alvorada com clorofilômetro portátil

The aims of this study was evaluated the efficiency of nitrogen sufficiency index (NSI) calculated with base on the measures relative chlorophyll index (RCI) leaves, measured with portable chlorophyll meter, as an indicator of time of sidedressing N fertilization and verify which value of NSI (90% and 95%) is the most appropriate to indicate the moment of N fertilization on common bean cultivar IAC Alvorada. An experiment with five N managements (M1: 200 kg ha(-1) N (40 kg at sowing + 80 kg at 15 days after emergency (DAE) + 80 kg 30 DAE); M2: 100 kg ha(-1) N (20 kg at sowing + 40 kg at 15 DAE + 40 kg at 30 DAE); M3: 20 kg ha(-1) N at sowing + 30 kg ha(-1) when chlorophyll meter readings indicated NSI < 95%; M4: 20 kg ha(-1) N at sowing + 30 kg N ha(-1) N when chlorophyll meter readings indicated NSI <90% and, M5: control (without N application)) and four replications was carried out in the rainy and dry growing seasons of the agricultural year 2009/10, in Botucatu, Sao Paulo State, Brazil. The variables RCI, aboveground dry matter, leaf N concentration, yield components, grain yield, and N use efficiency were evaluated. The NSI of 90%, calculated based on monitoring of RCI in common bean leaves with chlorophyll meter, was more effective than 95% to define need for N sidedressing in common bean cultivar IAC Alvorada, reducing the N application and improving the use efficiency of applied N.

Interação genótipo vs formas de inoculação com Azospirillum brasilense em milho

Pós-graduação em Agronomia (Genética e Melhoramento de Plantas) - FCAV

Critérios para o manejo da adubação nitrogenada na cultura do feijão utilizando clorofilômetro portátil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Growth and nutritional disorders of eggplant cultivated in nutrients solutions with suppressed macronutrients

The objective was to evaluate the effects of omitting macronutrients in the nutrients solution on growth characteristics and nutritional status of eggplants. The treatments were complete nutrients solution and solutions with nutrient omission: nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), and sulfur (S). The experiment was carried out under greenhouse conditions with three replicates in a completely random design. Plant height, number of leaves per plant, leaf area, relative chlorophyll index, photosynthesis rate, stomatal conductance, dry matter, concentration levels of macronutrients in plant aerial part and root system, and nutritional disorders were evaluated. Omitting elements interfered in the concentration of elements in the various plant tissues and this had as consequences limited vegetative growth, reduced dry matter and led to the development of the typical deficiency symptoms of each element. Although potassium was the most demanded of all elements, nitrogen and calcium were the most growth limiting ones.