Morphological and biochemical analysis of the stored and larval food of an obligate necrophagous bee, Trigona hypogea

Trigona hypogea, T. crassipes, and T. necrophaga are obligate necrophagous bees that differ from the majority of bees by using animal material instead of pollen as a protein resource. Since T. hypogea does not store protein in cerumen pots, it was thought that glandular secretions were its only larval protein source. This is in contrast to T. necrophaga which stores a yellowish proteinaceous jelly in the pots. Our results show that the larval food of T. hypogea has a higher protein content than the food stored in the pots and that it presents an electrophoretical protein pattern similar to that of the hypopharyngeal gland, indicating that workers add glandular secretions to the larval food while provisioning the brood cells. Thus, it can be suggested that T. hypogea has a provisioning behavior similar to other Meliponinae. The presence of several bands of proteins in the food stored in the pots shows that this species stores carrion mixed with honey in storage pots. Morphological data suggest that both larvae and adults make use of the same foodstuffs. These results also suggest that T. hypogea is more similar to other necrophagous species than it is to T. necrophaga (a more specialized bee).

Electrophoretic analysis of the protein in the midgut of stingless bees (Apidae; Meliponinae) with a comparison of necrophagous and feeding pollen workers

SDS electrophoresis of midgut proteins from two Trigonini species with different feeding habits shows many similarities and the absence of unique protein in the necrophagous stingless bee Trigona hypogea, suggesting that biochemical adaptation to necrophagy, in this bee, occurs more in the qualitative than in quantitative level. Workers of different ages display some variations in the midgut protein pattern, suggesting that it is important to know the age in studies regarding to bee digestion.

Ultrastructure of the midgut epithelium of Meliponinae larvae with different developmental stages and diets

The comparative study of the ultrastructure of the midgut epithelium of stingless bee larvae that eat plant protein (pollen) and animal protein (carrion) throughout the larval phase, shows variations in the digestive cells that are only relative to larval aging and not to the type of larval diet. The cells of older larvae present a cytoplasm with empty spaces that result from emptying of lipid and glycogen stocks, and the presence of autophagic vacuoles. These results are discussed in relation to the hypothesis that variations in the digestive tract of insects may be associated with different diets or phylogeny. We conclude that different diets do not determine cell morphology adaptations in the studied species. As the variations in the ultrastructure of the midgut epithelium are the same in all studied species, including the necrophagous species Trigona hypogea, throughout the larval stage, this sequence of changes seems to be due to different physiological state during larval development.

A specialized midgut region in stingless bees (Hymenoptera: Apidae: Meliponinae)

Ultrastructure of the anterior end of the midgut of three Neotropical stingless bees, including an obligate necrophagous species Trigona hypogea, suggests that this midgut region has no role in nutrient absorption because its lumen is reduced by a thick cuticle that lines the outer epithelium of the cardiac valve. A possible involvement of this midgut region in the production of the peritrophic membrane compounds is discussed.

A molecular phylogeny and the evolution of nest architecture and behavior in Trigona s.s. (Hymenoptera : Apidae : Meliponini)

Stingless bees exhibit extraordinary variation in nest architecture within and among species. To test for phylogenetic association of behavioral traits for species of the Neotropical stingless bee genus Trigona s.s., a phylogenetic hypothesis was generated by combining sequence data of 24 taxa from one mitochondrial (16S rRNA) and four nuclear gene fragments (long-wavelength rhodopsin copy 1 (opsin), elongation factor-1 alpha copy F2, arginine kinase, and 28S rRNA). Fifteen characteristics of the nest architecture were coded and tested for phylogenetic association. Several characters have significant phylogenetic signal, including type of nesting substrate, nest construction material, and hemipterophily, the tending of hemipteroid insects in exchange for sugar excretions. Phylogenetic independent habits encountered in Trigona s.s. include coprophily and necrophagy.

Flower constancy of the stingless bee Trigona carbonaria Smith (Hymenoptera : Apidae : Meliponini)

We tested the constancy of floral choice by Trigona carbonaria Smith in a garden by examining, using a scanning electron microscope, the composition of the pollen loads of individual foragers over time. Constancy was tested on three levels. Within a single trip, 88% of the samples examined comprised pure pollen loads (97% or more of one pollen type). Within a single day, 88% of bees visited the same species across trips sampled. Across 2 and 3 days, 82% and 73%, respectively, of individual bees foraged on a single pollen type. The majority of the remaining bees collected only two species of pollen. This pattern is consistent with that of other highly social bees. It enhances the pollinator efficacy of these insects by increasing the chances of pollen being transferred to stigmas of the same plant species. This increases the ecological importance of these bees and their value in crop pollination.

Acumulação de macronutrientes pelo amendoim(Arachis hypogea L.) cultivado no outono em época da seca

Plantas de amendoim da variedade Tatu (tipo vegetativo Valencia), cultivadas em um latossol-vermelho amarelo,foram colhidas em varios estágios de desenvolvimento. Sobre estas, foram determinadas concentrações e quantidades dos macronutrientes acumulados. Verificou-se que o nitrogênio, o potássio e o cálcio foram os nutrientes que mais se acumularam na planta. Quanto ao potássio, observou-se que este nutriente foi absorvido acen tuadamente apos o florescimento. Constatou-se um decréscimo na quantidade de enxofre na parte aerea apos o florescimento. As quantidades de nutrientes extraidos, em kg/ha, em base a uma população de 160.000 plantas/ha, foram, através das vagens (sementes + cascas): 142 kg N, 15 kg P, 30 kg K, 5 kg Ca, 10 kg Mg e 7,5 kg S e através da parte aerea ou vegetativa: 201 kg N, 16 kg P, 140 kg K, 113 kg Ca, 20 kg Mg e 16 kg S.

Notas sobre o tipo de Trigona limao Smith (Hymenoptera, Apidae, Lestrimelitta)

O nome limao tem sido usado para espécies distintas do gênero Lestrimelitta. O material-tipo de Smith depositado no The Natural History Museum (Londres) foi examinado. A operária de L. limao é descrita e o lectótipo para Trigona limao Smith, 1863 é designado. Esta espécie encontra-se restrita aos cerrados da porção central do Brasil, de São Paulo ao Maranhão. Distingue-se de outras espécies pelos seguintes caracteres diagnósticos: vértice com pouquíssimas cerdas eretas, muito finas e curtas, restritas à região interocelar; bordo anterior do mesoscuto com cerdas eretas castanhas nas laterais; laterais do mesepisterno com cerdas eretas esparsas e restritas à porção ventral e curvatura entre porções lateral e ventral, tornando-se mais longas próximo à base da coxa média; contorno da abertura do espiráculo propodeal, aproximadamente 2,5 x mais longo do que largo; flancos do propódeo coberto por pilosidade decumbente esbranquiçada, fina e ramificada; esporão mesotibial muito reduzido; tergo metassomal 1 com cerdas enegrecidas nas porções laterais.

Pollen storages in nests of bees of the genera Partamona, Scaura and Trigona (Hymenoptera, Apidae)

Bees and angiosperms established a mutualistic relationship along the evolutionary time. The aim of this study is to contribute for the understanding of this relation analyzing pollen stored by stingless bees colonies distributed along the Rio Negro. Fourteen species of Meliponini from the genera Partamona, Scaura, and Trigona were studied with regard to the content of pollen pots. The pollen material was removed from the pollen pots, homogenized, and prepared according to the usual acetolysis technique. The overlap of the trophic niche and the grouping of species by similarity of niches was calculated. The identification revealed 78 pollen types belonging to 36 families, being 37 types attractive and 16 considered as promoters of a temporary specialization event. With the results, it was possible to indicate a list of important plants for meliponiculture in the Amazon.

First record of the behavior of latex drainage by Trigona spinipes (Fabricius) (Hymenoptera, Apidae) in laticiferous flowers

This paper describes the behavior of the bee Trigona spinipes, to avoid the latex, when piercing the base of the tubular corolla of the flowers of Mandevilla guanabarica in order to steal the nectar.

Trigona spinipes (Fabr.) (Hymenoptera: Apidae) em espécies de maracujazeiro: flutuação populacional, horário de visitação e danos às flores

Avaliou-se a infestação, flutuação populacional e horário de visitação de Trigona spinipes (Fabr.) (Hymenoptera: Apidae) em espécies de maracujazeiro. As espécies utilizadas foram Passiflora coccinea, P. setacea, P. alata, P. edulis f. flavicarpa, P. laurifolia e P. nitida, aos três anos de idade. O ensaio foi conduzido em parcelas de 1,5 m de comprimento, avaliando-se os dois lados da espaldeira, considerando-se apenas o 0,5 m superior desta e totalizando, para os dois lados, a área de 1,5 m². Avaliaram-se os seguintes parâmetros: número de irapuás e porcentagem de dano nos botões florais, flores, frutos, ramos, folhas e pedúnculos. Para a determinação do horário de visitação, foi utilizada P. coccinea, sendo as avaliações realizadas às 9:00h, 12:00h e 15:00h. A correlação entre o número médio de T. spinipes presentes nas flores de espécies de maracujazeiro e a porcentagem de danos foi positiva e significativa (r = 0,99). em nenhuma das observações efetuadas, constatou-se a presença de T. spinipes nos botões florais, frutos, ramos, folhas ou pedúnculos das espécies de maracujazeiro, não ocorrendo danos nessas estruturas. Esse resultado sugere que as abelhas são atraídas pelas flores ocasionando danos nessas estruturas, provavelmente, por utilizar o tecido floral ou resinas contidas neste para a construção de ninhos. P. coccinea foi a espécie mais danificada por T. spinipes, apresentando suscetibilidade ao ataque desse inseto. Os horários de maior incidência de T. spinipes foram 9:00h e 12:00h, ocorrendo decréscimo significativo no número de abelhas por flor às 15:00h. Constatou-se maior infestação de T. spinipes nas flores das plantas de maracujá nos meses de outubro a novembro, coincidindo com a primavera, em Jaboticabal, SP.

Effect of larval food amount on ovariole development in queens of Trigona spinipes (Hymenoptera, Apinae)

Caste determination in Trigona spinipes Fabricius (Hymenoptera, Apidae, Meliponini) is trophogenic. Larvae that eat about 360 mu l of food become queens, while those who consume 36 mu l develop into workers. We studied the effect of larval nutrition on the number and length of ovarioles and on ovarian development in fifth instar larvae, white eyed, pink eyed and black-eyed pupae as well as newly emerged adults. All larvae have four ovarioles per ovary, while in queen pupae this number ranged from 8 to 15. Cyst formation, the cell death and other characteristics of ovary morphogenesis were the same regardless of the quantity of food consumed. These results are discussed in relation to caste differentiation in other bees.

Impact of Aqueous Plant Extracts on Trigona spinipes (Hymenoptera: Apidae)

The stingless bees are an important component of the insect biomass in many tropical areas, due to their collection of nectar and pollen. Trigona spinipes is a widely distributed species in South America, and described as a pollinator of many crops that can be used in a commercial pollinating system. The effects of plant extracts on insects are studied because of the demand for organic food and their selectivity to natural enemies. Plant insecticides are reported as a potential agent for the control of insect pests, however little is known about their impact on beneficial insects. This study investigated the survival of Trigona spinipes (Hymenoptera: Apidae, Meliponini) Fabricius, after exposure to the leaf extracts of Azadiracha indica (Meliaceae), Lippia sidoides (Verbenaceae), Sapindus saponaria (Sapindaceae), Anonna squamosa (Anonnaceae) Cymbopogon winterianum (Poaceae), Corimbia citriodora (Myrtaceae), Jatropha curcas (Euphorbiaceae) and Ricinus communis (Euphorbiaceae) and of seeds of Azadiracha indica, Ricinus communis Nordestina and AL Guarany varieties and Jatropha curcas. The extracts that had the greatest influence on the survival of the bees were A. indica at 3% and 7% of concentration, A. squamosa at a concentration of 10% with 68.89% survival and green leaf of R. communis at a concentration of 7%. The results show that although the extracts were effective in controlling pests, they may also affect the pollinator Trigona spinipes.

Análise faunística de himenópteros visitantes florais em fragmento de cerradão em Itirapina, SP

Bees are considered one of the most efficient pollinators. Therefore, they have Indisputable role in the plants reproduction, since they increase the quality and quantity of fruit and seeds, and thus they help the ecosystems maintenance. Cerrado is one of the most affected ecosystems due to the growth of human activities, drastically reducing its biodiversity. The faunistic analysis identifies the species and the size of the populations, and can also indicate the degree of environmental Impact on a particular area. Surveys on flower-visiting hymenoptera in a cerradao area, with 40ha, in the Experimental Station of Itiparina (SP), were conducted every fifteen days from March 2003 to February 2004. From 181 insects collected, the Apidae family was represented by the largest number of species and individuals. The species Apis mellifera (55.8%), Trigona spinipes (14.4%) and Exomalopsis (Exomalopsis) sp. (8.3%) were the most prevalent fit the area. Among the bees species collected, 30.8% were classified as sociable and 69.2% as solitary. Considering all hymenoptera collected, 59.7% preferred the morning period and 40.3% the afternoon period for foraging and/or visiting. The Diversity index (Shannon-Wiener) H was 1.6933, V(H) = 0.0123 and Uniformity index E = 0.5652, following pattern found in other areas of cerrado.

Olfactory eavesdropping between two competing stingless bee species

Foragers can improve search efficiency, and ultimately fitness, by using social information: cues and signals produced by other animals that indicate food location or quality. Social information use has been well studied in predator-prey systems, but its functioning within a trophic level remains poorly understood. Eavesdropping, use of signals by unintended recipients, is of particular interest because eavesdroppers may exert selective pressure on signaling systems. We provide the most complete study to date of eavesdropping between two competing social insect species by determining the glandular source and composition of a recruitment pheromone, and by examining reciprocal heterospecific responses to this signal. We tested eavesdropping between Trigona hyalinata and Trigona spinipes, two stingless bee species that compete for floral resources, exhibit a clear dominance hierarchy and recruit nestmates to high-quality food sources via pheromone trails. Gas chromatography-mass spectrometry of T. hyalinata recruitment pheromone revealed six carboxylic esters, the most common of which is octyl octanoate, the major component of T. spinipes recruitment pheromone. We demonstrate heterospecific detection of recruitment pheromones, which can influence heterospecific and conspecific scout orientation. Unexpectedly, the dominant T. hyalinata avoided T. spinipes pheromone in preference tests, while the subordinate T. spinipes showed neither attraction to nor avoidance of T. hyalinata pheromone. We suggest that stingless bees may seek to avoid conflict through their eavesdropping behavior, incorporating expected costs associated with a choice into the decision-making process.