Continuous Formation and Separation of Calcium-Alginate Capsules Containing Viable Mammalian Cells in Microfluidic Devices

Microfluidic devices can be used for many applications, including the formation of well-controlled emulsions. In this study, the capability to continuously create monodisperse droplets in a microfluidic device was used to form calcium-alginate capsules.Calcium-alginate capsules have many potential uses, such as immunoisolation of cells and microencapsulation of active drug ingredients or bitter agents in food or beverage products. The gelation of calcium-alginate capsules is achieved by crosslinking sodiumalginate with calcium ions. Calcium ions dissociated from calcium carbonate due to diffusion of acetic acid from a sunflower oil phase into an aqueous droplet containing sodium-alginate and calcium carbonate. After gelation, the capsules were separated from the continuous oil phase into an aqueous solution for use in biological applications. Typically, capsules are separated bycentrifugation, which can damage both the capsules and the encapsulated material. A passive method achieves separation without exposing the encapsulated material or the capsules to large mechanical forces, thereby preventing damage. To achieve passiveseparation, the use of a microfluidic device with opposing channel wa hydrophobicity was used to stabilize co-laminar flow of im of hydrophobicity is accomplished by defining one length of the channel with a hydrogel. The chosen hydrogel was poly (ethylene glycol) diacrylate, which adheres to the glass surface through the use of self-assembled monolayer of 3-(trichlorosilyl)-propyl methacrylate. Due to the difference in surface energy within the channel, the aqueous stream is stabilized near a hydrogel and the oil stream is stabilized near the thiolene based optical adhesive defining the opposing length of the channel. Passive separation with co-laminar flow has shown success in continuously separating calcium-alginatecapsules from an oil phase into an aqueous phase. In addition to successful formation and separation of calcium alginate capsules,encapsulation of Latex micro-beads and viable mammalian cells has been achieved. The viability of encapsulated mammalian cells was determined using a live/dead stain. The co-laminar flow device has also been demonstrated as a means of separating liquid-liquidemulsions.

O professor e sua formação: o impacto do PEC - formação universitária - ao professor da pessoa

Pós-graduação em Educação Escolar - FCLAR

Formação continuada de professoras de infância no Baixo Tocantins: concepções de criança [infância] e interdisciplinaridade

Os processos de formação continuada de professoras de infância têm ganhado importância no âmbito das políticas de educação no Brasil nos primeiros anos do século XXI. Seu principal objetivo tem sido proporcionar níveis de qualidade da atuação dos docentes que cuidam e educam as crianças menores de sete anos. Para combater o déficit histórico no plano do acesso e da qualidade, algumas iniciativas governamentais têm sido apresentadas à comunidade acadêmica numa tentativa de integração em rede. Afinal, qualquer ação que pretenda impactar na qualidade da formação dos professores em nível nacional exige a participação das universidades. Nesse sentido, a parceria entre os governos federal, estadual e municipal com as universidades tem buscado construir experiências consistentes, com ênfase na modalidade da educação a distância. Neste trabalho, discuto as concepções sobre criança, infância e interdisciplinaridade das professoras da região paraense do Baixo Tocantins a partir da atuação do Programa EDUCIMAT: Formação, Tecnologia e Serviços em Educação em Ciências e Matemática que tem por objetivo formar professores tutores capazes de mediar a formação continuada de outros professores no contexto do município. Para isso, construímos os dados da pesquisa por meio de questionários, entrevistas e registros escritos destacando as reflexões das professoras sobre suas memórias, concepções e práticas. O objetivo é ressaltar a importância da formação continuada para a transição conceitual vivenciada pelas professoras em formação. Concluo que o diálogo entre os fundamentos teóricos e as experiências educacionais das professoras possibilita um repensar de suas concepções e, por conseguinte, de suas práticas pedagógicas.

Oralidade e eventos de letramento relacionados à leitura: práticas sociais constitutivas da escrita

This research is the result of a senior research project and longed to verify if the ways of child‘s participation in orality in classroom and the literacy events related to reading would make possible to child construct a discursive relation with the writing. Besides it investigated the conception of 20 teachers from second year from primary school about orality and literacy and also identifies which theory purposes could be discerned in one of these teachers exercise. The methodology used was the qualitative approach, with exploratory- descriptive character, and based in system of references methodological of bibliographic research and collecting of facts research. The corpus of this research constituted in deflagrate element that stimulated the search for methodology answers which could clear the possible essence of set of problems evidenced. The results indicated that the ignorance of some basic conceptions that imbricate in teaching Portuguese language, principally in orality development, reading of writing by some individuals was alarming component parts, since many of them mediate the teachinglearning process of numberless individuals. Considering that orality and literacy play fundamental function in social interactions, this research has the intention to contribute for teacher‘s continuous formation, just as help them in development of students‘ linguistic competences, and elucidate them about the importance of literacy, besides to make public the more recently theoretical subsidies about this subject

A questão da licenciatura em Educação Fisica : a transição a pratica profissional

Universidade Estadual de Campinas. Faculdade de Educação Física

Da formação e desenvolvimento profissional do professor de educação fisica a inovação educativa

Universidade Estadual de Campinas . Faculdade de Educação Física

Formação, educação física e inclusão : compreendendo os processos inclusivos

No intuito de contribuir para a problematização da Educação, numa perspectiva inclusiva, esta dissertação procura conhecer e analisar a prática pedagógica inclusiva experimentada pelos professores de Educação Física na escola, por meio de suas narrativas, em uma ação de formação continuada. O projeto de extensão intitulado Formação Continuada de Professores de Educação Física para a Educação Inclusiva foi a estratégia utilizada para a criação de um grupo de estudos, denominado Grupo Operativo de Formação (GOF). A ação de formação continuada foi realizada durante o período de julho a dezembro de 2011, em um encontro presencial semanal, totalizando dezesseis encontros, com uma carga horária final de 90h. Participaram da ação de formação quatro professores da Secretaria Municipal de Educação de Vitória-ES. A pesquisa baseia-se em elementos da pesquisa-ação, utilizando como instrumentos e procedimentos para a produção dos dados a narrativa, o diário de campo, o memorial e a entrevista. Da análise dos dados produzidos identifica três categorias norteadoras das discussões: a) o trabalho coletivo como possibilidade para inclusão; b) trilhando caminhos para o desenvolvimento de práticas inclusivas: o autismo como foco do percurso; c) o olhar dos professores sobre a ação de formação experimentada no GOF. As análises realizadas revelam que essa ação de formação traz resultados que sinalizam positivamente para sua efetivação como instrumento ativo no processo de formação de professores para a educação básica, na perspectiva da inclusão. Portanto, essa é uma interessante ferramenta a ser utilizada pelas redes de ensino como alternativa metodológica em programas de formação continuada. Além disso, a proposta promove momentos para a prática reflexiva dos professores, representando uma rica oportunidade, capaz de fazer com que eles se apropriem de teorias educacionais que poderão contribuir na elaboração de suas aulas.

O fim de um ciclo? A coconstrução da articulação curricular entre o 1.º e o 2.º Ciclos do Ensino Básico

Dissertação Apresentada à Escola Superior de Educação de Lisboa para obtenção de grau de mestre em Ciências da Educação - Especialidade Supervisão em Educação

Medindo valor em comunidades de prática: caso multicurso

Communities of Practice are groups of people who share a concern, problem or passion about a topic, and who deepen their knowledge and expertise by interacting regularly. It¿s use as a tool for planning and implementation of programs for continuous training of teachers in Brazil is not widespread, but used occasionally. This dissertation seeks to demonstrate the use of the communities of practice theory in continuous teachers training in the Brazilian reality. It presents some models of continuous formation currently in process in Brazil, points to the principles of knowledge management taken by this work, presents the communities of practice theory and the criticism of this in its theoretical part. For the case study was used the methodology described by Robert Yin (2005) of single case study to examine the Multicurso, program to continuously train math teachers in the state of Goiás, Brazil. The community of practice formed in this program was examined on its form, as to the cultivation of communities of practice, as to the development and about the challenges of geographical distribution. Going beyond, this work has as its main objective to propose a methodology for measuring the value of communities of practice from the case studied, without the intention to make generalizations about the measurement of value in projects of various kinds. The model proposed indicators separated into two categories, the outcome and impact, to facilitate the measurement of value and prevent that communities of practice are to be criticized or super estimated by assignment of value to those without the unequivocal proof of unique causality at the same time it searchs to attest that the contribution of communities of practice in improvements in the systems in which the CoP are in.

Um estudo sobre a aprendizagem de números irracionais no ensino médio

The present study describes theoretical practical relationships between development and application of activities in Mathematics education. It s proposed a methodological approach to Mathematics in the first grade of Ensino Médio, supported by an experiment involving Irrational Numbers education by using constructive activities, applied obeying an educational sequence. Constructivism is used as an important theoretical reference in teaching learning process of Mathematics. The methodological intervention was done with two classes of students of the first grade of Ensino Médio, in two public schools, a state one and a federal one, located on the city of Natal, Rio Grande do Norte. The development, application and testing of the activities used on this experiment led us to think more profoundly about the value of constructivism ideas and understand that the use of activities that obey an educational sequence favors the learning. It s also discussed the research results, commented on a way to contribute to the advances of the proposal and it s more constant use. The participation and testing of the students were analyzed and judged using Skemp s Instrumental Understanding and Relational Understanding concepts. The results of the research were considered good, so we believe this methodological intervention can be used more frequently in the classes of Ensino Médio and also be applied to teachers in courses of initial education and continuous formation

Formação continuada de professores de ciências para o ensino de astronomia nos anos iniciais do ensino fundamental

Situated at the intersection of Continuing Formation, Science Teaching and content of astronomy, the research aims to discuss this problematic : What are the challenges encountered in a continuous formation in service of science teachers of the early years of elementary school with contents of astronomy? Aiming to answer this question, we carried out a collaborative action research in a school of Natal / RN, with 6 teachers who teach or lectured Natural Sciences in the early years of elementary school. The study involved 14 encounters of continuos formation in service, to enable a better understanding criticism and propositive concerning the teaching science, especially some content of astronomy for the initial years, through a shared discussion. To this end, we consider that more profound understanding of astronomical knowledge allows the establishment of relationships and connections between theoretical knowledge and the daily teaching practice. All discussions were recorded on audio and transcripts. This research was realized using questionnaires and diary, however, the audio recording was its principal working instrument. About the data collected, several issues emerged as the little field of conceptual content, several misconceptions among teachers about the content of astronomy, lack of adequate space in the school environment to study and the need for collective reflection on the practice. Finally, is indicated that the contents of astronomy should be used in classrooms of Sciences, provided that teaching plan of action consider the existence of diverse alternative conceptions about the subject, among both teachers and students, and the need of permanente continuing education in the teaching

A prática pedagógica de professores de alunos com transtorno de déficit de atenção/hiperativadade

This study is inserted at the Line of Strategical Research of Thinking and Knowledge production which scientific projects about the relation of thinking and knowledge production are realized. The accomplishment of this dissertation involved an empiric research at a school of the municipal district of Natal RN. Our purpose was to investigate the practice of the staff working with students who present Disabilities and Attention Deficit Hyperactivity (DADH) at regular classes due to the organization of thinking in pedagogical strategies. The object of this study is presented at the center of the questions which involves the conscious analysis of the problems and needs that emerge at the school. Considering the specialty of the theme, we choose a methodology whose focus is the dialogue and the sharing of meanings with the partners of this research through the observation of the activities developed at class/school and interviews/conversations with six teachers of first and second cycles of primary education. According with the study, some theoretician presumptions of Mr. Freire (2001), Mr. Nóvoa (1995), Mr. Bohm (2005) and son on. The results reveled at the research indicate the fragility of a continuous formation directed to the development of critical-reflexive thinking of the teachers.The teachers revel conceptions due to formation, pedagogical practices and the relation with the parents and coordinators, through their performances and speeches, allowing us to identify some pedagogical strategies used. We identified some negative response about the process of learning-developing of these pedagogical strategies such as the one we call unconcern . The strategy of sitting the student at the front row chairs can have positive and negative responses depending on the way the teacher act and follow the student. Other strategies identified as positive response bringers at the learning-developing process and that should be reinforced by the staff are the playful and the group assignments . At this perspective, the school needs to develop a collective project between the pedagogical team and teachers to overcome the needs of all students, and as a consequence, of the staff and improve the positive strategies, minimizing the negative ones and allowing the organizations of new strategies that promotes the improvement of learning-teaching process of the students with DADH

A fábrica de professores e a padronização do conhecimento

This paper concern the general subject of teacher career construction, since the period of the initial formation in the teaching course of pedagogy crossing the first effectives experiences of professional performance, until the reflections about the results of the continuous formation propositions. The specifics subject is the formative quotidian in the teacher s life beyond of the traditional stereotypes about individuals and institutions bring up for discussion a micro sociological approach about the professionals uncertainty and the personal hopes, the teacher displeasures and the pedagogical satisfactions. The epistemological reflection about the different formatives experiences, taking as example the Programa de Formação de Alfabetizadores PROFA (Alphabetizers Formation Program) developed by the Secretaria Municipal de Educação of Natal City, consider the ambiguous tension between the knowledge that the teachers acquire in their formation and the concrete teachers practical since those experiences. The study methodology is based in the qualitative comprehensive research, sustained by observations, impressive reflective accounts, and also in the theoretic analyses with contemporary references to the critical pedagogical through and education sociology. I used the autobiographic reflective account by the writer-researcher in her owner academic and professional way. I propose an image of a teacher s factory in such a way for its conformist aspects to the continuity stands as much the internal contradictions of the system. Gears, pieces and manual instructions are the educational components of this factory as ideological, institutional and technical structures to aim at to limit the critics and the changes. However the teachers thinking and acting with their owner creativity to adapted uncontexted professional knowledge s to their pedagogical lives. Under those analogies I propose the problem of this research the teacher s formation and theirs pedagogical practices- and also examine briefly its theoretic-philosophical and methodological basis. From the analysis and interpretation of my owner experiential life as teacher s educator I put the necessity of re-thinking the teacher s formation in terms to rebound more meaningfully in their pedagogical actuation. I believe an important part of the teacher s formation process is the personal wish to learn more about the office, explicitly connected to human convictions and values such as to a positive professional identity. Thus, the teacher able to reflect about your owner educator life can find the best way to help the students to think critically about their culture and history, valorizing creative exits by themselves to face the conformist social stands connected with school education

Entrando na dança: reflexos de um curso de formação continuada para professores de educação infantil

Pós-graduação em Artes - IA

Novas formas de pensar/dizer, dizer/pensar: a imagem como linguagem mediadora da experiência do formador de professores

Pós-graduação em Artes - IA