Beryllofluoride mimics phosphorylation of NtrC and other bacterial response regulators

Two-component systems, sensor kinase-response regulator pairs, dominate bacterial signal transduction. Regulation is exerted by phosphorylation of an Asp in receiver domains of response regulators. Lability of the acyl phosphate linkage has limited structure determination for the active, phosphorylated forms of receiver domains. As assessed by both functional and structural criteria, beryllofluoride yields an excellent analogue of aspartyl phosphate in response regulator NtrC, a bacterial enhancer-binding protein. Beryllofluoride also appears to activate the chemotaxis, sporulation, osmosensing, and nitrate/nitrite response regulators CheY, Spo0F, OmpR, and NarL, respectively. NMR spectroscopic studies indicate that beryllofluoride will facilitate both biochemical and structural characterization of the active forms of receiver domains.

Map of the Huron River Valley from Surveys made in 1905-08 by Gardner Stewart Williams [Map 5109]

Argo Pond , ca. 1905-1909: Map of the Huron River Valley

Map of the Huron River Valley from Surveys made in 1905 by Gardner Stewart Williams [Map 5107]

Arboretum, 1905: Map of the Huron River Valley

Map of the Huron River Valley from Surveys made in 1905 by Gardner Stewart Williams [Map 5106]

Geddes Pond, 1905: Map of the Huron River Valley

Map of the Huron River Valley from Surveys made in 1907 by Gardner Stewart Williams [Map 5105]

Vivienne Farm, 1907: Map of the Huron River Valley

Map of the Huron River Valley from Surveys made in 1907 by Gardner Stewart Williams [Map 5104]

Fleming Creek, 1907: Map of the Huron River Valley

Map of the Huron River Valley from Surveys made in 1906-07 by Gardner Stewart Williams [Map 5103]

Superior Pond, ca. 1906-1907: Map of the Huron River Valley

Map of the Huron River Valley from Surveys made in 1907 by Gardner Stewart Williams [Map 5102]

Leforge Road, 1907: Map of the Huron River Valley

Map of the Huron River Valley from Surveys made in 1907 by Gardner Stewart Williams [Map 5101]

Ypsilanti, 1907: Map of the Huron River Valley

Map of the Huron River Valley from Surveys made in 1905-1909, by Gardner Stewart Williams.

Ann Arbor, ca. 1905-1909: Map of the Huron River Valley, revised 5/11/1915

Report to the government of Saskatchewan on wheat marketing,

Mode of access: Internet.

Map of the Huron River Valley from Surveys made in 1909 by G. S. Williams. 5123 [Tuttle Hill Road-Harris Road]

Tuttle Hill Road-Harris Road, 1909: Map of the Huron River Valley

Map of the Huron River Valley from Surveys made in 1909 by G. S. Williams. 5122 [Whittaker Road-Huron River Drive]

Whittaker Road-Huron River Drive, 1909: Map of the Huron River Valley

Map of the Huron River Valley from Surveys made in 1908 by G. S. Williams. 5126 [Belleville West]

Belleville West, 1908: Map of the Huron River Valley

Map of the Huron River Valley from Surveys made in 1908 by G. S. Williams. 5125 [Rawsonville East]

Rawsonville East, 1908: Map of the Huron River Valley