L'ecole et la vie pour un adolescent de 14-15 ans. 'La escuela y la vida para un adolescente de 14-15 años'.

Proyecto financiado a través del programa Sócrates Comenius 1.1. Otros centros que participaron en el proyecto: I.E.S. Gabriel y Galán (Plasencia, España), Collège 'Les Gondoliers' (La Roche sur Yon, Francia), 2ième Gymnase (Livadia, Grecia), Dominican Collège (Portstewart, Irlanda del Norte), Scuola Media 'A cavallari' (Portomaggiore, Italia) Escola Secundaria Nuno Alvares (Castelo Branco, Portugal) y Lycée Jacques Yves Cousteau (Saint Pétersbourg, Rusia)

Mechanisms underlying the vasorelaxant action of the pimarane ent-8(14),15-pimaradien-3 beta-ol in the isolated rat aorta

Pimarane-type diterpenes were described to exert antispasmodic and relaxant activities. Based on this observation we hypothesized that the diterpene ent-8(14),15-pimaradien-3 beta-ol (PA-3 beta-ol) induced vascular relaxation. With this purpose, the present work investigates the mechanisms involved in the vasorelaxant effect of the pimarane-type diterpene PA-3 beta-ol. Vascular reactivity experiments, using standard muscle bath procedures, were performed in isolated aortic rings from male Wistar rats. Cytosolic calcium concentration ([Ca(2+)]c) was measured by confocal microscopy using the fluorescent probe Fluo-3AM. PA-3 beta-ol (10, 50 and 100 mu mol/l) inhibited phenylephrine and KCl-induced contraction in either endothelium-intact or denuded rat aortic rings. PA-3 beta-ol also reduced CaCl(2)-induced contraction in Ca(2+)-free solution containing KCl (30 mmol/l) or phenylephrine (0.1 mu mol/l). PA-3 beta-ol (1-300 mu mol/l) concentration dependently relaxed phenylephrine-pre-contracted rings with intact or denuded endothelium. The diterpene also relaxed KCl-pre-contracted rings with intact or denuded endothelium. Moreover, Ca(2+) mobilization study showed that PA-3 beta-ol (100 mu mol/l) and verapamil (1 mu mol/l) inhibited the increase in Ca(2+)-concentration in smooth muscle and endothelial cells induced by phenylephrine (10 mu mol/l) or KCl (60 mmol/l). Pre-incubation of intact or denuded aortic rings with N(G)-nitro-L-arginine methyl ester (L-NAME, 100 mu mol/l) and 1H-[1,2,4] Oxadiazolo[4,3-a]quinoxalin-1-one (ODQ 1 mu mol/l) produced a rightward displacement of the PA-3 beta-ol concentration-response curves. On the other hand, 7-nitroindazole (100 mu mol/l), 1400 W (1 mu mol/l), indomethacin (10 mu mol/l) and tetraethylammonium (1 mmol/l) did not affect PA-3 beta-ol-induced relaxation. Collectively, our results provide evidence that the effects elicited by PA-3 beta-ol involve extracellular Ca(2+) influx blockade. Its effects are also partly mediated by the activation of NO-cGMP pathway. (C) 2009 Elsevier B.V. All rights reserved.

Hominoid fission of chromosome 14/15 and the role of segmental duplications.

Ape chromosomes homologous to human chromosomes 14 and 15 were generated by a fission event of an ancestral submetacentric chromosome, where the two chromosomes were joined head-to-tail. The hominoid ancestral chromosome most closely resembles the macaque chromosome 7. In this work, we provide insights into the evolution of human chromosomes 14 and 15, performing a comparative study between macaque boundary region 14/15 and the orthologous human regions. We construct a 1.6-Mb contig of macaque BAC clones in the region orthologous to the ancestral hominoid fission site and use it to define the structural changes that occurred on human 14q pericentromeric and 15q subtelomeric regions. We characterize the novel euchromatin-heterochromatin transition region (∼20 Mb) acquired during the neocentromere establishment on chromosome 14, and find it was mainly derived through pericentromeric duplications from ancestral hominoid chromosomes homologous to human 2q14-qter and 10. Further, we show a relationship between evolutionary hotspots and low-copy repeat loci for chromosome 15, revealing a possible role of segmental duplications not only in mediating but also in "stitching" together rearrangement breakpoints.

Operación Eureka XXXII, 14-15 febrero, 1975

Expone los resultados de la Operación Eureka XXXII, realizado a fin de obtener información sobre el reclutamiento 1975, así como otros aspectos biológicos y de medio ambiente, que como parte del sistema de monitoreo del recurso anchoveta realizó el Instituto del Mar del Perú.

Operación Búsqueda de Peces Reclutas (14-15 Noviembre 1974)

Expone los resultados de la operación realizada durante los días 14 y 15 de noviembre en la que participaron 31 bolicheras que exploraron la costa entre 4° y 18°L.S, con el fin de determinar la distribución y concentración de los peces reclutas provenientes del desove de invierno-primavera 1974 para efectuar así un estimado de su abundancia.

Tutkimuksen raaka-aineita ja monitieteisiä reseptejä : : III Kulttuurintutkimuksen päivät 14.-15.12.2007 Joensuu

Ajankohtaista

Suomen Pyöräilymestaruuskilpailut Helsingissä 14-15 p. heinäkuuta 1934

14 x 22 cm

Ratsastusseuran ja Suomen Kenttäratsastusklubin Kampin kentällä toukokuun 14-15 p:nä 1927 järjestämien ratsastuskilpailujen ohjelma

15 x 40 cm