Annexin A2 and PSF proteins interact with p53 IRES and regulate translation of p53 mRNA

p53 mRNA has been shown to be translated into two isoforms, full-length p53 (FL-p53) and a truncated isoform Delta N-p53, which modulates the functions of FL-p53 and also has independent functions. Previously, we have shown that translation of p53 and Delta N-p53 can be initiated at Internal Ribosome Entry Sites (IRES). These two IRESs were shown to regulate the translation of p53 and Delta N-p53 in a distinct cell-cycle phase-dependent manner. Earlier observations from our laboratory also suggest that the structural integrity of the p53 RNA is critical for IRES function and is compromised by mutations that affect the structure as well as RNA protein interactions. In the current study, using RNA affinity approach we have identified Annexin A2 and PTB associated Splicing Factor (PSF/SFPQ) as novel ITAFs for p53 IRESs. We have showed that the purified Annexin A2 and PSF proteins specifically bind to p53 IRES elements. Interestingly, in the presence of calcium ions Annexin A2 showed increased binding with p53 IRES. Immunopulldown experiments suggest that these two proteins associate with p53 mRNA ex vivo as well. Partial knockdown of Annexin A2 and PSF showed decrease in p53 IRES activity and reduced levels of both the p53 isoforms. More importantly the interplay between Annexin A2, PSF and PTB proteins for binding to p53mRNA appears to play a crucial role in IRES function. Taken together, our observations suggest pivotal role of two new trans-acting factors in regulating the p53-IRES function, which in turn influences the synthesis of p53 isoforms.

Digital ‘lens’ on the UK PSF: supporting the digital capabilities of teaching staff in UK HE

This resource can also be used by professional staff who are seeking accreditation via the UK PSF for relevant aspects of their role, e.g. staff who support the use of technologies for learning and/or support the development of digital literacies.

O trabalho do assistente social no Programa Saúde da Família (PSF) de Ipatinga/MG : limites e perspectivas

Esta dissertação analisa o trabalho do Assistente Social no Programa Saúde da Família PSF, tomando por base o município de Ipatinga-MG. Buscou-se mostrar a concepção de parte da gestão local da Política de Saúde e dos profissionais da equipe básica do programa sobre o trabalho do Assistente Social na saúde e no PSF, e se esse profissional tem conseguido realizar um trabalho que contribua para a efetivação do programa enquanto estratégia de reorientação do modelo assistencial na atenção básica. O estudo foi desenvolvido através da metodologia de estudo caso, com enfoque qualitativo. A coleta de dados se deu por meio de consultas documentais e bibliográficas sobre a Política de Saúde e PSF a nível nacional e municipal; visitas às unidades básicas de saúde e a realização de entrevistas com uma porcentagem dos gerentes, dos profissionais da equipe básica e com os Assistentes Sociais. O estudo apontou que, o profissional de Serviço Social não vem conseguindo realizar um trabalho em conjunto com o Saúde da Família, se limitando aos atendimentos de casos isolados, assumindo papel complementar e subsidiário aos profissionais da equipe básica, não conseguindo, assim, contribuir para a concretização do programa enquanto estratégia que traga mudanças na organização dos serviços de atenção básica em saúde no município.

O efeito da implantação do programa saúde da família (PSF) nas taxas de procedimentos odontológicos em municípios do Estado do Rio de Janeiro

O objetivo deste estudo foi comparar as taxas de implantação das Equipes de Saúde Bucal, dos 92 municípios do estado do Rio de Janeiro, com as taxas dos procedimentos odontológicos de primeira consulta, procedimentos preventivos e procedimentos básicos dos mesmos municípios, verificando o período de 1998 a 2010. Os resultados, obtidos através de dados secundários fornecidos pelas fontes de dados do Ministério da Saúde, mostraram que o crescimento nas taxas dos procedimentos odontológicos foi muito mais modesto do que o crescimento verificado nas taxas de cobertura das Equipes de Saúde Bucal no período estudado e que, após a implantação dessas equipes, também não se observou impacto do aumento dessa implantação nas taxas de procedimentos odontológicos. Os únicos procedimentos que parecem ter alguma relação com a implantação dessas equipes são os procedimentos preventivos. Concluiu-se que, pelo menos no estado do Rio de Janeiro, não se pode afirmar que as Equipes de Saúde Bucal tenham melhorado significativamente o acesso aos serviços odontológicos da população em anos recentes e que isso serve de alerta para que, antes de mais investimentos no aumento do número de Equipes de Saúde Bucal, haja esforços no sentido de avaliar por que esse programa não tem proporcionado o aumento no acesso esperado.

Da mudança de modelo ao modelo de mudanças: um estudo sobre o curso introdutório do PSF

O Programa de Saúde da Família, criado em 1994, vem aumentando sua importância no cenário das políticas de saúde desde então. Em 2006, a política nacional de atenção básica adota suas diretrizes como eixo para sua própria estruturação. Este trabalho tem por objetivo estudar o processo de produção e disseminação do antagonismo PSF versus o modelo tradicional. Para tanto, foram entrevistados quatro profissionais que já atuaram como gestores do programa no município do Rio de Janeiro ou como integrantes de equipe de PSF em outras cidades do país. Os resultados das entrevistas foram analisados à luz da técnica da análise de conteúdo de Bardin. Os resultados apontam uma clara intenção dos gestores de gerar uma mobilização entre os participantes do curso introdutório de PSF, no sentido de combater prováveis resistências que estes últimos deverão enfrentar nos seus cotidianos.

Processo de trabalho em saúde bucal e necessidades de educação permanente: a experiência no PSF do Município de Petrópolis

A inserção das Equipes de Saúde Bucal no Programa de Saúde da Família (PSF) representa um desafio na mudança do modelo de atenção, tradicionalmente centrado no indivíduo e na doença para uma abordagem integral do indivíduo inserido em seu contexto familiar, comunitário e social. A formação do cirurgião-dentista, eminentemente técnica e voltada para o mercado privado, não contribui para subsidiar o serviço público com profissionais preparados, com conhecimento da realidade social e necessidades de saúde da população usuária do Sistema Único de Saúde. A presente pesquisa teve como objetivo principal conhecer o processo de trabalho das Equipes de Saúde Bucal no Programa de Saúde da Família do Município de Petrópolis, a partir das perspectivas dos profissionais cirurgiões-dentistas e identificar as tendências no manejo de situações-problema emergentes, no intuito de contribuir para o processo de Educação Permanente em Saúde. Com caráter exploratório e qualitativo, os métodos empregados foram a entrevista em grupo no formato de Grupo Focal, e inquérito realizado com o universo dos profissionais para caracterizar as atividades realizadas pelos cirurgiões-dentistas no PSF. Subsidiariamente foram incluídas informações provenientes de participação observante da autora. A análise do inquérito permite caracterização das atividades dos cirurgiões-dentistas no PSF, cuja visibilidade é ainda restrita, apontando condições de trabalho que permitem a realização da maioria das ações preconizadas nos documentos oficiais. A análise dos Grupos Focais pode subsidiar iniciativas de Educação Continuada e Permanente, destacando-se as seguintes temáticas: trabalho em equipe, organização e planejamento da assistência clínica e das ações coletivas, fortalecimento da supervisão, ferramentas para realização de diagnóstico coletivo e apoio à integração da saúde bucal ao processo de trabalho no Programa de Saúde da Família.

环形光栅光学扫描全息系统的OTF和PSF

于2010-11-23批量导入

[PSF-PDMS-PHS]_n/PSF共混物的相容性及表面组成

利用ＤＳＣ、ＤＭＡ、ＴＥＭ和ＸＰＳ对［ＰＳＦ－ＰＤＭＳ－ＰＨＳ］ｎ／ＰＳＦ共混物的相容性及表面组成进行了研究．结果表明，ＰＤＭＳ在共混物表面的富集与ＰＳＦ均聚物和［ＰＳＦ－ＰＤＭＳ－ＰＨＳ］ｎ中硬段的相容性有关；ＰＤＭＳ在相容的共混物体系表面的富集与对应的多嵌段共聚物组成基本相近；不相容共混物体系表面ＰＤＭＳ的富集程度相对较高，当共混物本体中有机硅含量从１％增至５％，表面层ＰＤＭＳ的含量迅速增加，可达到嵌段共聚物中ＰＤＭＳ的含量．

含有机硅三元多嵌段共聚物的研究──Ⅱ．（PSF－PDMS－PHS）_n的动态力学性能研究

以双酚Ａ聚砜（ＰＳＦ）为硬段、聚对羟基苯乙烯（ＰＨＳ）为半硬段、聚二甲基硅氧烷（ＰＤＭＳ）为软段合成了三元多嵌段共聚物，并对其动态力学性能和抗张性能进行了比较详细的研究，结果表明该三元多嵌段共聚物为多相结构；在引入半硬段ＰＨＳ后两相间的界面相增宽，粘接力增大，其强度和模量高且稳定，使含有机硅高分子材料强度低的弱点得以改善。扭辩分析（ＴＢＡ）结果显示，这种三元多嵌段共聚物具有多重转变，且在－２０℃至５０℃间有两个较大的几乎重叠的吸收峰；这表明这种三元多嵌段共聚物的相分离结构较为复杂，并有良好的阻尼性能。

Prática do enfermeiro do Programa Saúde da Família PSF na promoção da saúde do adolescente

Attention to the health of adolescents, based on paradigm flexneriano, needs to be overcome. Since the International Conference of Ottawa (1986), the literature is developing a discussion of the promotion of health, based on the paradigm of social production in health, suggests a design to overcome the health care traditional practices. Program Health of the Family PSF has this purpose to transmute the model of existing assistance, where the nurse is an essential element to the work done in the program. Around this context, it is our purpose to analyze the practice of nurse of the PSF for the promotion of health of adolescents, produced by a search of descriptive quantitative approach with the inclusion of qualitative data. Interviews were conducted with 9 nurses 3 units of health of the family USF, Mossoró-RN and applied questionnaires with 74 teenagers aged between 15 and 19 years old, with some nearby public schools where USF operate these nurses. The quantitative descriptions were transformed on tables, pictures and graphics using the program Excel (Microsoft) and the qualitative were worked through the technique of analyzing the content of Bardin (2004). The review was realized using the reference to promote health brought by the study. The results show that the most common problems that happen with teenagers are the drugs (33,8%), pregnancy (27,0%) and political problems-socio-economic-cultural issues (24,3%). Adolescents are spontaneous demand and rarely seek the USF. The actions presented by the nurses as, lectures and groups, are nothing comparing to the macro-problems presented by adolescents, and verticalized irregular. The nurses know the promotion of health generally, not explaining how operate it from its daily practice. Concluded that the practice of nurse of the PSF has not yet reach the promotion of health of the adolescent, being necessary to scheduling modules on the subject to continuous training of teams, professionals from USF, as well as teachers and other staff of schools, giving space to the participation of academic. The discussions should be socialized with the community to discuss possibilities of confrontation of the problems, which also require socio-structural changes. This research can contribute as work-diagnosis, which experienced the reality of care in nursing PSF to a specific group

Atividades preventivas realizadas pelos cirurgiões-dentistas do PSF de Natal-RN

Family Health Program (FHP) presents itself as a restructured model on basic cares besides having prevention practices and promotion in health as essential parts. According to that, the purpose of this study is to perceive which prevention practices in oral health are accomplished by the dentists from the FHP of Natal-RN as well as the way they have been developed, the knowledge basis to execute the procedure and the use of evaluation instruments by the dentists and by the Oral Health Co-Ordinator. A list of dentists participating on the FHP of Natal in March, 2006 (n= 91) was obtained so it was possible to formulate a structured interview. After excluding those with less than six months attending to the program, and considering the loss, eighty dentists got to be interviewed. It had questions about individual and group preventive procedures, sources of basement to develop the activities and to verify the impact and evaluation instruments. Besides that, an interview was made with the Oral Health Co-Ordinator about the evaluating process and the existence of a protocol as well as about document analysis in the Ambulatory Informations System Unique Health System (AIS-UHS), Information of Basic Care System and Pact of Recorder. The main individual activities consist on Oral Hygiene Orientation (87,5%) and Fluoridization (95%). Group activities remain at scholar groups acts(91,25%) being fluoridization done by 91,25% and educative activities by 86,25% mainly through speeches (61,25%).Orientations about oral cancer were detached on the aged group (39,96%)and hypertensive and diabetic group (19,51%), as well as integration with the health team was respectively 21,93% and 39,02%. Speeches and talk rounds are the main activities for aged, hypertensive and diabetics, and are also expressive to pregnant women. For pregnant women and babies group predominate hygiene and diet orientation. About the evaluation instruments 73,75% of the dentists do not consider their existence, despite 73,75% consider that actions have impact specially due to a increase of hygiene and decrease of caries index on the children. The main sources of basement are clinical experience (42,5%) and preparative courses (33,75%), only 7,5% mentioned population need and local reality. The Oral Health Co-Ordinator related the Attention Pact Recorder and records from the AIS - UHS as evaluation source but also detached the lack of epidemiologic surveying. She mentioned the increase of supervised brushing as a positive result and detached the hardness of the professional to act inside the family and as a team. The AIS-UHS records showed that fluoridization is more used individually and the supervised brushing is predominant as a group action, besides that educative activities at the Health Establishment predominate due to actions at the community. Facing the results, it was verified the act in different groups, special attention given to scholars, as well as focus the activities on caries giving less emphasis to other oral problems. Despite that, there was no epidemiologic instruments or data of the local reality to guide the actions and to be part of the planning and evaluation process

A educação em saúde pública na práqtica cotidiana: atuação dos cirurgiões-dentistas do PSF de Natal-RN

Considering education a support to health promotion, care integration and citizenship formation,the purpose of this research was to analyze the perception of the oral surgeons from the Family Health Program of Natal-RN over education in health as well as their performance as educators based on their activities on the program. A qualitative study was accomplished by a semi-structured interview and a Free Association of Words Test with 80 oral surgeons from the Family Health Program of Natal-RN. The instruments were analyzed through the meaning analysis and the Central Nucleus of Vergès Theory. The results showed a lack of planning in health actions so there is no standardization on the educative practices done by the oral surgeons which mostly are focused on scholars. There was an agreement among the group according to the oral surgeons´ perception about education in health that education is related to its function of recall prevention ideas to the population. Most part of the context units analyzed by the professionals´ speech show the knowledge of education in health as an inadequate behavior change instrument of the individuals. An interesting point was a quotation cited by some professionals that included actual themes such as citizenship, motivation and life quality, put inside the speech of education in health. To the oral surgeons the biggest difficulties on the development of the educative actions are due to the lack of incentive by the Municipal Health Bureau and to the detachment and lack of valorization of the themes by the population. The oral surgeons consider themselves co-responsible for the formation of a population which is able to request its health. They also mention the knowledge about the need of the community participation on the planning of the Family Health Program actions. Finally, it is notable the need for more encouragement so the oral surgeons can be more capable and have more interest in applying education in health on the perspective of a new model in health, because once capable and stimulated they can awake the population to education importance as a great transformation instrument for people searching for a fair, equalitarian and citizeness society