Binary vectors for sense and antisense expression of arabidopsis ESTs

Our laboratory is interested in devising methods to identify functions for the vast numbers of arabidopsis genes now available. For this purpose, we have constructed a set of binary vectors that will allow the quick production of transgenic arabidopsis plants containing either sense or antisense copies of EST clones obtained from the PRL2 library. These vectors are based on the pSLJ series containing the bialophos resistance (BAR) gene that confers resistance to the herbicide BASTA. Tn addition, our vectors contain a 35S CaMV promoter-polylinker-nos terminator cassette that allows the direct cloning of arabidopsis ESTs in either antisense (pAOV and pAOV2) or sense (pSOV and pSOV2) orientation. We also describe the construction of two additional vectors conferring BASTA resistance and containing the pBluescript polylinker in both orientations inserted between the 35S CaMV promoter and nos terminator (pKMB and pSMB).

Expressed sequence tags (ESTs) from the salivary glands of the tick Amblyomma cajennense (Acari : Ixodidae)

The neotropical tick Amblyomma cajennense is a significant pest to domestic animals, the most frequently human-biting tick in South America and the main vector of Brazilian spotted fever (caused by Rickettsia rickettsii), a deadly human disease. The purpose of this study is to characterize the adult A. cajennense salivary gland transcriptome by expressed sequence tags (ESTs). We report the analysis of 1754 clones obtained from a cDNA library, which reveal mainly transcripts related to proteins involved in the hemostatic processes, especially proteases and their inhibitors. Remarkably, five types of possible serine protease inhibitors were found, including a molecule with a distinguished structure that contains repeats of the active motif of hirudin inhibitors. Besides, other components that may be active over the host immune system or acting as defensins against infecting microorganisms were also described, including a molecule similar to insect venom allergens. The conjunction of components from this transcriptome suggests a diverse strategy of A. cajennense tick during feeding, but emphasized in the coagulation system. (c) 2008 Published by Elsevier Ltd.

Identification of Transcribed Sequences (ESTs) in the Trypanosoma cruzi Genome Project

Random single pass sequencing of cDNA fragments, also known as generation of Expressed Sequence Tags (ESTs), has been highly successful in the study of the gene content of higher organisms, and forms an integral part of most genome projects, with the objective to identify new genes and targets for disease control and prevention and to generate mapping probes. In the Trypanosoma cruzi genome project, EST sequencing has also been a starting point, and here we report data on the first 797 sequences obtained, partly from a CL Brener epimastigote non-normalized library, partly on a normalized library. Only around 30% of the sequences obtained showed similarity with Genbank and dbEST databases, half of which with sequences already reported for T. cruzi.

¿Estás ahí? : Duelo y luto por un joven muerto a través de Facebook

Después de la muerte de Toni, en agosto de 2011, sus amigos y conocidos continuaron dirigiéndole mensajes en su cuenta de Facebook. Este trabajo presenta un estudio cualitativo, desde una perspectiva etnográfica, de esa cuenta de Facebook, como un lugar virtual que permitió nuevas formas de duelo, luto y relación con la memoria de la persona fallecida, ante la desaparición o pérdida de significado de los ritos tradicionales relacionados con la muerte.

¿Estás en la onda? Aplicación de Praat a la enseñanza de la entonación

En este trabajo se pretende, por una parte, analizar cuáles son los métodos y programas existentes que se utilizan en la enseñanza de la entonación asistida por ordenador. Por otra parte, se pretende proporcionar algunas ideas para la aplicación del soporte visual en la enseñanza de la entonación

Eucalyptus ESTs involved in mechanisms against plant pathogens and environmental stresses

Eucalypt plantation has high economical importance in Brazil; however, it has been attacked by various pathogens under different environmental stress conditions. Disease resistance and survival under unfavorable environmental conditions have revealed that the eucalypt has developed highly efficient defense systems. Here we show the results of the Eucalyptus ESTs Genome Project (FORESTs). Using the expressed sequence tags (ESTs) obtained by the Project, contigs of similar sequences from each cDNA library induced and not induced by stress agents were formed, and cDNA sequences similar to other already known molecules, such as plant-signaling molecules, phytoalexins, lignin biosynthesis pathways, PR-proteins and putative genes corresponding to enzymes involved in the detoxification of reactive oxygen species, were identified. We also present general considerations about the mechanisms of Eucalyptus defense against biotic and abiotic stresses. These data are of extreme importance for future eucalypt breeding programs aimed at developing plants with enhanced resistance against pathogens and environmental stresses.

« Cy apres commencent les croniques de France abregiées : comment le royaulme de Gaules fut commanciez et depuis nommez France, et comment la cité de Lutesse fut construite et depuis nommée Paris, et aussi parle de tous les roys qui y ont estés, et dont ils sont premierement venus, et de pluseurs autres qui d'eulx sont descendus; par especial depuis la destruction de Troye la Grant et jusques en l'an de grace Nostre Seigneur Jhesu Crist mil quatre cens et trente, et depuis » jusqu'en 1506. (Fol. 1 à 21).

Contient : 1 Quatre vers, dont le premier est ainsi : « Debat contre mes debateurs » ; 2 « Par le roy en son conseil, monseigneur le constable present. Blondeau »

I tu? Ja estàs conectat?. '¿Y tu? ¿Ya estás conectado?'.

En este artículo el autor pretende motivar a profesores a tener la voluntad de ser creativos buscando distintas aplicaciones didácticas y educativas en el mundo de las nuevas tecnologías, ya que considera que se utilizan muy poco los recursos que estas tecnologías aportan a la escuela. No se puede dejar a los alumnos solos ante la pantalla, por lo que se les debe enseñar a hacer un buen uso de estas tecnologías. Éste es, según el autor, uno de los objetivos que deben tener los maestros de hoy en día.

¿Estás on line, amiguito?

Resumen tomado de la publicación. Monográfico con el título: Nuevas tecnologías y educación en medios

¿Me estás escuchando? : cómo conversar con niños entre los 4 y los 12 años.

Resumen basado en el de la publicación

Donde quieras que estés, comunicaré contigo.

Título del encuentro: 'Niños y adolescentes en el aula de ELE', organizado por el Instituto Cervantes de Nápoles

Transcriptome analysis of Taenia solium cysticerci using Open Reading Frame ESTs (ORESTES)

Background: Human infection by the pork tapeworm Taenia solium affects more than 50 million people worldwide, particularly in underdeveloped and developing countries. Cysticercosis which arises from larval encystation can be life threatening and difficult to treat. Here, we investigate for the first time the transcriptome of the clinically relevant cysticerci larval form. Results: Using Expressed Sequence Tags (ESTs) produced by the ORESTES method, a total of 1,520 high quality ESTs were generated from 20 ORESTES cDNA mini-libraries and its analysis revealed fragments of genes with promising applications including 51 ESTs matching antigens previously described in other species, as well as 113 sequences representing proteins with potential extracellular localization, with obvious applications for immune-diagnosis or vaccine development. Conclusion: The set of sequences described here will contribute to deciphering the expression profile of this important parasite and will be informative for the genome assembly and annotation, as well as for studies of intra- and inter-specific sequence variability. Genes of interest for developing new diagnostic and therapeutic tools are described and discussed.

Eucalyptus ESTs involved in mechanisms against plant pathogens and environmental stresses

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Satellyptus: analysis and database of microsatellites from ESTs of Eucalyptus

The main goal of our research was to search for SSRs in the Eucalyptus EST FORESTs database (using a software for mining SSR-motifs). With this objective, we created a database for cataloging Eucalyptus EST-derived SSRs, and developed a bioinformatics tool, named Satellyptus, for finding and analyzing microsatellites in the Eucalyptus EST database. The search for microsatellites in the FORESTs database containing 71,115 Eucalyptus EST sequences (52.09 Mb) revealed 20,530 SSRs in 15,621 ESTs. The SSR abundance detected on the Eucalyptus ESTs database (29% or one microsatellite every four sequences) is considered very high for plants. Amongst the categories of SSR motifs, the dimeric (37%) and trimeric ones (33%) predominated. The AG/CT motif was the most frequent (35.15%) followed by the trimeric CCG/CGG (12.81%). From a random sample of 1,217 sequences, 343 microsatellites in 265 SSR-containing sequences were identified. Approximately 48% of these ESTs containing microsatellites were homologous to proteins with known biological function. Most of the microsatellites detected in Eucalyptus ESTs were positioned at either the 5 or 3 end. Our next priority involves the design of flanking primers for codominant SSR loci, which could lead to the development of a set of microsatellite-based markers suitable for marker-assisted Eucalyptus breeding programs.

PR gene families of citrus: their organ specific-biotic and abiotic inducible expression profiles based on ESTs approach

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)