962 resultados para Artawazd I, King of Armenia, 149 B.C.
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Half title: Artawazd.
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Mode of access: Internet.
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Human c-sis/PDGF-B proto-oncogene has been shown to be overexpressed in a large percentage of human tumor cells establishing a growth-promoting, autocrine growth circuit. Triplex forming oligonucleotides (TFOs) can recognize and bind sequences in duplex DNA, and have received considerable attention because of their potential for targeting specific genomic sites. The c-sis/PDGF-B promoter contains a unique homopurine/homopyrimidine sequence (SIS proximal element, SPE), which is crucial for binding nuclear factors that provoke transcription. In order to develop specific transcriptional inhibitors of the human c-sis/PDGF-B proto-oncogene, 20 potential TFOs targeting part or all of the SPE were screened by gel mobility analysis. DNase I footprinting shows that the TFOs we designed can form a sequence-specific triplex with the target. Protein binding assays demonstrate that triplex formation inhibits nuclear factors binding the c-sis/PDGF-B promoter. Both transient and stable transfection experiments demonstrate that the transcriptional activity of the promoter is considerably inhibited by the TFOs. We propose that TFOs represent a therapeutic potential to specifically diminish the expression of c-sis/PDGF-B proto-oncogene in various pathologic settings where constitutive expression of this gene has been observed.
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"List of authorities": v. 1, p. 540-553.
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Includes biographical references and index.
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Includes index.
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Mode of access: Internet.
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Includes bibliographical references.
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Mode of access: Internet.
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Sabin 58729; Wagner 70.
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Originally published (1751-66) as separate works. The life of Peters appeared without the author's name.
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Vols. 3-5 published by H. Colburn and R. Bently.
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Mode of access: Internet.
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Mode of access: Internet.