Detection of the peanut allergen Ara h 6 in foodstuffs using a voltammetric biosensing approach

A voltammetric biosensor for Ara h 6 (a peanut allergen) detection in food samples was developed. Gold nanoparticle-modified screen-printed carbon electrodes were used to develop a sandwich-type immunoassay using two-monoclonal antibodies. The antibody-antigen interaction was detected through the electrochemical detection of enzymatically deposited silver. The immunosensor presented a linear range between 1 and 100 ng/ml, as well as high precision (inter-day RSD ≤9.8 %) and accuracy (recoveries ≥96.7 %). The detection and quantification limits were 0.27 and 0.88 ng/ml, respectively. It was possible to detect small levels of Ara h 6 in complex food matrices.

Ara sortim! : 6. '¡Ahora salimos! : 6'.

Contiene seis visitas guiadas: la primera tiene como finalidad el conocimiento de la fauna autóctona de Vallcalent, la segunda, lleva a cabo el reconocimiento de la Vall de Núria. Seguidamente, se realiza una salida al aire libre en un jardín botánico, luego, nos presentan una actividad referida al mar y el submarinismo, y ya para finalizar, nos conducen al Canal Olímpico para llevar acabo las distintas actividades deportivas que permite la instalación (navegación, tiro con arco) y el estudio del ecosistema submarino.

Incidence of ipsilateral postoperative deep venous thrombosis in the amputated lower extremity of patients with peripheral obstructive arterial disease

Objective: Patients undergoing amputation of the lower limb due to peripheral arterial disease (PAD) are at risk of developing deep venous thrombosis (DVT). Few studies in the research literature report the incidence of DVT during the early postoperative period or the risk factors for the development of DVT in the amputation stump. This prospective study evaluated the incidence of DVT during the first 35 postoperative days in patients who had undergone amputation of the lower extremity due to PAD and its relation to comorbidities and death. Methods: Between September 2004 and March 2006, 56 patients (29 men), with a mean age of 67.25 years, underwent 62 amputations, comprising 36 below knee amputations (BKA) and 26 above knee amputations (AKA). Echo-Doppler scanning was performed preoperatively and on postoperative days 7 and 31 (approximately). All patients received acetylsalicylic acid (100 mg daily) preoperatively and postoperatively, but none received prophylactic anticoagulation. Results: DVT occurred in 25.8% of extremities with amputations (10 ARA and 6 BKA). The cumulative incidence in the 35-day postoperative period was 28% (Kaplan-Meier). There was a significant difference (P = .04) in the incidence of DVT between AKA (37.5%) and BKA (21.2%). Age >= 70 years (48.9% vs 16.8%, P = .021) was also a risk factor for DVT in the univariate analysis. Of the 16 cases, 14 (87.5%) were diagnosed during outpatient care. The time to discharge after amputation was averaged 6.11 days in-hospital stay (range, 1-56 days). One symptomatic nonfatal pulmonary embolism occurred in a patient already diagnosed with DVT. There was no relation between other comorbidities and DVT. The multivariate analysis showed no association between risk factors and the occurrence of DVT in the amputated extremity. DVT ipsilateral to the amputation did not influence the mortality rate (9.7%). Conclusion: The incidence of DVT in the early postoperative period (<= 35 days) was elevated principally in patients aged >= 70 years and for AKA. Patients with PAD who have recently undergone major amputations should be considered at high risk for DVT, even after hospital discharge. Given the high rate of postoperative DVT observed in this study, we now recommend prophylactic anticoagulation for these patients, but further study is needed to determine the optimal duration and efficacy of this treatment. (J Vasc Surg 2008;48:1514-9.)

BIOMETRIA DE FRUTOS DE ARAÇÁ-BOI (EUGENIA STIPITATA MC VAUGH)

Neste trabalho são apresentados dados referentes a biometria de frutos de araçá-boi (Eugenia stipitata Mc Vaugh). Pelos dados obtidos, observa-se que os frutos de araça-boi apresentam uma grande variação quanto ao peso total (média 161diâmetro (média 7,25 cm), comprimento (média 6,08 cm), número de sementes (média 12), peso das sementes (média 33,58 g), peso da casca (média 25,63 g) e peso da polpa (média 102,30 g). As variáveis estudadas, em geral, foram correlacionadas positiva e significativamente, podenso serem utilizadas para estimar uma outra variável, isoladamente.

Calagem e adubação fosfatada para formação de mudas de araçá-boi

O araçá-boi (Eugenia stipitata McVaugh) é uma espécie frutífera cultivada em escala doméstica na região Amazônica. Entretanto, pouco se conhece sobre seu requerimento nutricional na fase de muda. O objetivo deste trabalho foi avaliar o efeito da calagem e da adubação fosfatada sobre o crescimento e nutrição de mudas de araçá-boi. O experimento foi conduzido em viveiro, com amostras de um Latossolo Amarelo distrófico, em delineamento experimental de blocos casualizados e esquema fatorial 5 x 5: cinco doses de calcário (0; 1,37; 2,83; 4,29 e 5,75 g por muda) e cinco de fósforo (0, 100, 200, 400 e 600 mg kg-1 de P), com quatro repetições. Dez meses após o transplante, foram avaliados o crescimento em altura e em diâmetro, a matéria seca da parte aérea e das raízes e o acúmulo de nutrientes na parte aérea das plantas. As doses de calcário influenciaram significativamente a altura, o diâmetro, a matéria seca da parte aérea, das raízes e total das mudas. As doses de fósforo influenciaram apenas o crescimento em diâmetro a matéria seca da parte aérea e total. Houve efeito significativo das doses de calcário e de fósforo sobre o acúmulo de macro e micronutrientes na parte aérea das plantas, exceto para o Cu, influenciado apenas pelas doses de calcário. O melhor desenvolvimento das mudas de araçá-boi pode ser obtido com a aplicação de até 3,77 g de calcário e 6,21 g de superfosfato triplo por muda.