A collagen-poly(lactic acid-co-ɛ-caprolactone) hybrid scaffold for bladder tissue regeneration.

Scaffold materials should favor cell attachment and proliferation, and provide designable 3D structures with appropriate mechanical strength. Collagen matrices have proven to be beneficial scaffolds for tissue regeneration. However, apart from small intestinal submucosa, they offer a limited mechanical strength even if crosslinking can enhance their mechanical properties. A more cell-friendly way to increase material strength is to combine synthetic polymer meshes with plastic compressed collagen gels. This work describes the potential of plastic compressed collagen-poly(lactic acid-co-ɛ-caprolactone) (PLAC) hybrids as scaffolds for bladder tissue regeneration. Human bladder smooth muscle and urothelial cells were cultured on and inside collagen-PLAC hybrids in vitro. Scaffolds were analyzed by electron microscopy, histology, immunohistochemistry, and AlamarBlue assay. Both cell types proliferated in and on the hybrid, forming dense cell layers on top after two weeks. Furthermore, hybrids were implanted subcutaneously in the backs of nude mice. Host cell infiltration, scaffold degradation, and the presence of the seeded bladder cells were analyzed. Hybrids showed a lower inflammatory reaction in vivo than PLAC meshes alone, and first signs of polymer degradation were visible at six months. Collagen-PLAC hybrids have potential for bladder tissue regeneration, as they show efficient cell seeding, proliferation, and good mechanical properties.

In vitro and in vivo ocular biocompatibility of electrospun poly(ɛ-caprolactone) nanofibers.

Biocompatibility is a requirement for the development of nanofibers for ophthalmic applications. In this study, nanofibers were elaborated using poly(ε-caprolactone) via electrospinning. The ocular biocompatibility of this material was investigated. MIO-M1 and ARPE-19 cell cultures were incubated with nanofibers and cellular responses were monitored by viability and morphology. The in vitro biocompatibility revealed that the nanofibers were not cytotoxic to the ocular cells. These cells exposed to the nanofibers proliferated and formed an organized monolayer. ARPE-19 and MIO-M1 cells were capable of expressing GFAP, respectively, demonstrating their functionality. Nanofibers were inserted into the vitreous cavity of the rat's eye for 10days and the in vivo biocompatibility was investigated using Optical Coherence Tomography (OCT), histology and measuring the expression of pro-inflammatory genes (IL-1β, TNF-α, VEGF and iNOS) (real-time PCR). The OCT and the histological analyzes exhibited the preserved architecture of the tissues of the eye. The biomaterial did not elicit an inflammatory reaction and pro-inflammatory cytokines were not expressed by the retinal cells, and the other posterior tissues of the eye. Results from the biocompatibility studies indicated that the nanofibers exhibited a high degree of cellular biocompatibility and short-term intraocular tolerance, indicating that they might be applied as drug carrier for ophthalmic use.

Arkistoluettelo 603: Tapio Lipponen

Luettelo Kansalliskirjastossa olevan Tapio Lipposen arkiston sisällöstä

Decreto rectoral No. 603 :Apruébase la extensión de la Especialización en Derecho Financiero en convenio con la Universidad de San Buenaventura de Cartegena

Que es política de la Universidad hacer presencia académica en el país. Que la Universidad de San Buenaventura, de la ciudad de Cartegena, tiene interés en ofrecer en asocio con el Colegio Mayor de Nuestra Señora del Rosario el programa en Especialización en Derecho Financiero.

Revista del Colegio Mayor de Nuestra Señora del Rosario Número 603

La Revista del Rosario es una publicación institucional que busca hacer visible a la Universidad ante la comunidad interna (estudiantes, docentes y directivas), y externa (padres de familia, alumnos de Colegios Convenio, empresas amigas, donantes) en cuatro ejes: docencia, investigación, extensión y cultura y patrimonio. Asimismo, se piensa como un espacio para la expresión de las diferentes visiones de los miembros de nuestra comunidad, frente a los diversos temas que conforman la vida nacional.

Na 50 Nachlass Arthur Schopenhauer - 'Schopenhauer-Archiv', 603 - Angebot zur Veröffentlichung der Preisschrift

u.a.: Hamburger Verlagsfirma Perthes, Besser & Mauke;

Ms.Ff.F.Stoltze 3. 603 - Brief von Heinrich Stoltze an Marie Stoltze

Familie

Na 1 Nachlass Max Horkheimer, 603 - Habilitationsschrift: "Über Kants Kritik an der Urteilskraft als Bindeglied zwischen theoretischer und praktischer Philosophie"; Kollegheft zu einer Vorlesung von K. Goldstein über Gehirnpsychologie (p. VII 7 - VII 11)

"Über Kants Kritik der Urteilskraft als Bindeglied zwischen theoretischer und praktischer Philosophie", Habilitationsschrift, Typoskript, gebunden, 109 Blatt (GS 2, S. 74-146); Zur Habilitation, "Referat Habilitationsschrift", Typoskript, 2 Blatt (GS 11, S. 76-79); Zur Habilitation, "Lebenslauf", Typoskript, 4 Blatt; "Über Husserls erkenntnistheoretische Fundierung der Wesensschau", Habilitations- Probevortrag, vom 27.02.1925, Manuskript, 1 Heft, 18 Blatt, davon 4 leer, 1 zusätzliches Blatt (GS 11, S. 80-99); Kollegheft zu einer Vorlesung von K. Goldstein über Gehirnpsychologie" eigenhändige Notizen, 1 Heft, 19 Blatt, davon 1 leer; Über Kant, Vorlesungsskript einer Begleitveranstaltung zu einer Vorlesung von Hans Cornelius, enthält auch Notizen zu einer Vorlesung über die Anfänge der bürgerlichen Philosophie, Manuskript, 1 Heft, 11 Blatt, Datierung unklar;