Questing one Brazilian query: reporting 16 cases of Q fever from Minas Gerais, Brazil

Q fever has been considered non-existing in Brazil where reports of clinical cases still cannot be found. This case-series of 16 patients is a result of a systematic search for such illness by means of clinical and serologic criteria. Serologic testing was performed by the indirect microimmunofluorescence technique using phase I/II C. burnetii antigens. Influenza-like syndrome was the most frequent clinical form (eight cases - 50%), followed by pneumonia, FUO (fever of unknown origin), mono-like syndrome (two cases - 12.5% each), lymphadenitis (one case - 6.3%) and spondylodiscitis associated with osteomyelitis (one case - 6.3%). The ages varied from four to 67 years old with a median of 43.5. All but one patient had positive serologic tests for phase II IgG whether or not associated with IgM positivity compatible with acute infection. One patient had both phase I and phase II IgG antibodies compatible with chronic Q fever. Seroconvertion was detected in 10 patients. Despite the known limitations of serologic diagnosis, the cases here reported should encourage Brazilian doctors to include Q fever as an indigenous cause of febrile illness.

A química newtoniana

The work of Newton exerted a profound influence on the development of science. In chemistry this newtonian influence was present in Query 31 of Newton's Optics. However, the incursion of Newton's thought into chemistry brought upon the chemists an epistemological question, that of the nature of their discipline. Would chemistry be a discipline in its own right, or simply a branch of physics? In this work we present the newtonian program for chemistry, as well as the reaction of traditional chemists to it. We conclude by proposing that Lavoisier carried through a synthesis between newtonian methodology and the singularity of traditional chemistry.

A significação conceitual pela escrita e reescrita orientada em aulas de Química

In this paper, the practice of guided writing and rewriting in Chemistry classes on a graduate degree in Science is shown as a teaching method that promotes conceptual meaning in Chemistry among students. The practice examined involves writing and rewriting of texts by the students- a process guided by the teacher - about main concepts in Chemistry. The experience, follow up of classes with students' reports and the results obtained confirm that the practice of guided writing and rewriting promotes conceptual meaning in Chemistry and a good level of learning.

pp-Blast: a "pseudo-parallel" Blast

We have developed a software called pp-Blast that uses the publicly available Blast package and PVM (parallel virtual machine) to partition a multi-sequence query across a set of nodes with replicated or shared databases. Benchmark tests show that pp-Blast running in a cluster of 14 PCs outperformed conventional Blast running in large servers. In addition, using pp-Blast and the cluster we were able to map all human cDNAs onto the draft of the human genome in less than 6 days. We propose here that the cost/benefit ratio of pp-Blast makes it appropriate for large-scale sequence analysis. The source code and configuration files for pp-Blast are available at http://www.ludwig.org.br/biocomp/tools/pp-blast.