SPATIAL DISTRIBUTION OF PHLEBOTOMINAE IN PUERTO IGUAZU-MISIONES, ARGENTINA-BRAZIL-PARAGUAY BORDER AREA

SUMMARY The first Argentinian autochthonous human case of visceral leishmaniasis (VL) was confirmed in Posadas (Misiones) in 2006. Since then, the disease has increased its incidence and geographical distribution. In the 2006-2012 period, 107 human cases were detected (11 deaths). The presence of Lutzomyia longipalpis was detected in peridomiciles in Puerto Iguazú urban area in 2010; some of these findings were associated with households where cases of canine VL had already been reported. The objective of this study was to ascertain the abundance and spatial distribution of Lu. longipalpis in Puerto Iguazú City, on the Argentina-Brazil-Paraguay border. Lu. longipalpis proved to be exclusively urban and was found in 31% of the households sampled (n = 53), 67% of which belonged to areas of low abundance, 20% to areas of moderate abundance and 13% to areas of high abundance. Nyssomyia whitmani was the only species found both in urban and peri-urban environments, and Migonemyia migonei was registered only on the outskirts of the city. Due to the fact that Puerto Iguazú is considered to be at moderate risk at the moment, it is necessary to intensify human and canine case controls, as well as take integrated prevention and control measures regarding the environment, vectors and reservoirs on the Argentina-Brazil-Paraguay border area.

VERTICAL TRANSMISSION OF DENGUE VIRUS IN Aedes aegypti COLLECTED IN PUERTO IGUAZÚ, MISIONES, ARGENTINA

A finding of vertical transmission of the DEN 3 virus in male specimens of Aedes aegypti, collected in the 2009 fall-winter period, in Puerto Iguazú city, Misiones, Argentina, using the RT-PCR technique in a 15-specimen pool is reported. This result is analyzed within the context of the epidemiological situation of Argentina's northeast border.

IDENTIFICATION OF Leishmania infantum IN PUERTO IGUAZÚ, MISIONES, ARGENTINA

 The emergence of zoonotic visceral leishmaniasis (ZVL) in Latin America is a growing public health problem. The urbanization of ZVL has been observed in different countries around the world, and there are a growing number of reports drawing attention to the emergence of this infection in new locations, as well as its increase in previously established areas of endemicity. In the city of Posadas, Misiones province, Northeastern Argentina, the transmission of ZVL associated with canines and Lutzomyia longipalpis was first reported in 2006. In the city of Puerto Iguazú, also in Misiones province, the first human case of ZVL was reported in February 2014. From 209 surveyed dogs, 15 (7.17%) were identified as positive by serological and/or parasitological methods. Amplification was observed in 14 samples and in all cases the species implicated was Leishmania infantum. To the authors’ knowledge, this is the first molecular characterization of L. infantum from dogs in this area.

Cytokine responses to Dengue Infection among Puerto Rican Patients

Recently, a strong correlation between high concentration of tumor necrosis factor (TNFalpha) in blood and severity of dengue hemorrhagic fever/dengue shock syndrome has been reported from Asia and the Pacific. We wished to determine if a similar relationship could be found in dengue patients in the Americas where adult patients with severe syndromes have been observed more frequently than in Asia where severe cases have been observed mostly among children. The concentrations of interleukin-1 (IL-1beta) in hospistalized adult groups were significantly lower than that in outpatient adults. In contrast, the levels of interleukin 6 (IL-6) were significantly higher in hospistalized adults and children than in the corresponding outpatients. Levels of TNFalpha were higher in hospistalized children than in outpatient children or hospistalized adults. There was no significant difference in the levels of these three cytokines among hospitalized patients with or without hemorrhagic manifestations. Thus, an elevated IL-6 level was positively associated with severity of dengue infection in both children and adults, but IL-1beta level was negatively associated with severity in adults.

First published record of urban malaria in Puerto Gaitan, Meta, Colombia

Patterns of malaria cases were compared between the department of Meta and the municipality of Puerto Gaitán, Colombia, to examine temporal change in malaria from 2005-2010. During this time frame in Meta the mean ratio was 2.53; in contrast, in Puerto Gaitán it was 1.41, meaning that a surprisingly high proportion of Plasmodium falciparum cases were reported from this municipality. A detailed analysis of data from Puerto Gaitán for 2009 and 2010 detected a significant difference (χ2, p < 0.001) in the distribution of plasmodia, with Plasmodium vivax more prevalent in 2009 and P. falciparum in 2010. Males had the highest number of cases but there was no difference in the distribution of cases between sexes and years. In both years, for both sexes, people 16-40 accounted for the majority of cases (58.9% in 2009; 60.4% in 2010). There were significant differences in the distribution of both P. vivax (χ2, p < 0.01) and P. falciparum cases (χ2, p < 0.05) by geographic setting (urban vs. non-urban) between years. Urban cases of both P. vivax and P. falciparum are recorded in this study for the first time in Puerto Gaitán, possibly the result of area wide displacement and migration due to armed conflict.

New distribution record of Cybocephalus kathrynae (Coleoptera, Cybocephalidae) on Mona Island, Puerto Rico

New distribution record of Cybocephalus Kathrynae (Coleoptera, Cybocephalidae) on Mona Island, Puerto Rico. A new record of Cybocephalus kathrynae T.R. Smith (Cybocephalidae) is reported for Puerto Rico. Adults were collected from the flowers of Mammillaria nivosa (Cactaceae) on Mona Island Reserve. Prior to this study, this beetle species was only reported for Monroe and Miami-Dade Counties, Florida, USA.

Conhecimentos, atitudes e prática do exame de Papanicolaou entre mulheres argentinas

OBJETIVO: Avaliar os conhecimentos, atitudes e prática acerca do exame de Papanicolaou e verificar sua associação com variáveis sociodemográficas entre mulheres. MÉTODOS: Por meio de inquérito domiciliar foram entrevistadas 200 mulheres da localidade de Puerto Leoni, Misiones, Argentina, selecionadas de forma aleatória simples. As respostas foram descritas quanto ao conhecimento, atitude e prática, e suas respectivas adequações para o exame de Papanicolaou, previamente definidas. A adequação foi comparada entre as categorias das variáveis de controle pelo teste chi2, com nível de significância de 5%. RESULTADOS: O conhecimento e prática do exame de Papanicolaou foram adequados em 49,5% e 30,5% das entrevistadas, respectivamente, embora a atitude frente ao exame tenha sido considerada adequada em 80,5% das entrevistadas. A falta de solicitação pelo médico ou por outros profissionais de saúde foi referida por 58,9% das mulheres como principal motivo para não realização do exame. CONCLUSÕES: Os resultados revelaram a necessidade, sobretudo entre os profissionais de saúde, de fornecerem mais informações sobre o exame, gerando conhecimento à população sobre as vantagens e benefícios do exame Papanicolaou.

Experimental infection with Schistosoma mansoni of Biomphalaria straminea from different parts of the Northeast of Brazil

In the western part of the State of Bahia Biomphalaria straminea and B. glabrata both occur, but in the majority of cases they do not share the same habitat. In the State of Ceará, however, B. straminea is the sole snail host of Schistosoma mansoni. In this survey, no naturally infected B. straminea was found among snails collected from Bahia and Ceará, evidently because of the very low infection rates. The susceptibility of laboratory-reared specimens to infection with a Puerto Rican strain of S. mansoni was then tested experimentally. In general, the snails showed very low susceptibility. The infection rates were 1.1% among snails from Redenção (Ceará); 2.3% in those from Pentecoste (Ceará); 2.9% in snails from São Desidério (Bahia), while they were very high among an albino strain (NIH) of B. glabrata used as control. Another group of B. straminea from São Desidério was exposed to a Bahian strain of S. mansoni and the infection rate was still very low (3.6%) Apparently, the very low susceptibility of B. straminea, despite high snail density, is correlated with moderate infection rates with S. mansoni among humans, as shown by the results of stool examinations conducted by SUCAM in the municipalities of Redenção and Pentecoste, in Ceará.

Strain variation in the infectivity of Schistosoma mansoni for Biomphalaria glabrata

Five strains of Schistosoma mansoni resistant and susceptible to schistosomicides were studied for infectivity of 2 strains of Biomphalaria glabrata one of Puerto Rican origin and the other of Brazilian origin. Puerto Rican strains of S. Mansoni developed more slowly and had a lower infectivity in Brazilian B. glabrata than did the Brazilian S. mansoni. However, Brazilian S. Mansoni developed as well in Puerto Rican snails as in Brazilian snails, indicating that drug resistant strains could easily be moved by travel of infected persons from one area to another.

Identificação de rotavirus associado ao sorotipo G2 em Yucatan, México

Neste estudo reporta-se a identificação de rotavirus sorotipo G2 proveniente de amostras fecais de crianças com gastroenterite, da Cidade de Mérida, Yucatán, México. O diagnóstico virológico foi feito através da eletroforese em gel de poliacrilamidia e ensaio imunoenzimático. Das 149 amostras estudadas, 25 (16,7%) foram positivas para rotavirus do grupo A; dessas 23 (92%) foram sorotipo G2, subgrupo I e padrão eletroforético curto e 2 (8%) subgrupo II e padrão eletroforético longo, porém, não foi possível determinar o sorotipo G. Desde 1985, até hoje, foi a única vez que o sorotipo G2 foi identificado, em mais de 90% das amostras analisadas. Acredita-se que, provavelmente, nos últimos anos tem acumulado na população, um grupo de pessoas suscetíveis ao sorotipo de RV, e num futuro poderia ter um surto importante de gastroenterite, associado ao sorotipo G2 de rotavirus.

Serological and parasitological study and report of the first case of human babesiosis in Colombia

A study on the presence of Babesia in humans was performed in Puerto Berrío (Latitude 6.50deg. Longitude: -74.38deg. River: Magdalena. Area: 74.410km², Colombia-South America). Indirect immunofluorescence, thin and thick blood smears were used to study 194 individuals. Patients were grouped according to their risk-factors for Babesia infection: (group 1) individuals with fever, chills, sweating and other malaria-type symptoms; (group 2) symptomatic and asymptomatic individuals from local cattle ranches, which were enrolled in an active form, and (group 3) workers from the local slaughterhouse. Seven individuals were serologically positive for Babesia: Three individuals presented IgM antibodies against B. bovis, while one had IgG against this species; one individual had IgM against B. bigemina, another had IgG and a third both IgM and IgG against this species. Only one individual was parasitologically positive for Babesiaand serologically positive for Babesia bovis (IgM 1:64)

Simultaneous occurrence of brain tumor and myeloradiculopathy in schistosomiasis mansoni: case report

Simultaneous occurrence of brain tumor and myeloradiculopathy in cases of Manson's schistosomiasis have only rarely been described. We report the case of a 38-year-old man who developed seizures during a trip to Puerto Rico and in whom a brain tumor was diagnosed by magnetic resonance imaging: brain biopsy revealed the diagnosis of schistosomiasis. He was transferred to a hospital in the United States and, during hospitalization, he developed sudden paraplegia. The diagnosis of myeloradiculopathy was confirmed at that time. He was administered praziquantel and steroids. The brain tumor disappeared, but the patient was left with paraplegia and fecal and urinary dysfunction. He has now been followed up in Brazil for one year, and his clinical state, imaging examinations and laboratory tests are presented here.

Notas e descrições em Parandrini (Coleoptera, Cerambycidae, Parandrinae)

The genus Parandra is reviewed and four genera are recognized: Parandra Latreille, 1804, Neandra Lameere, 1912, stat. nov., Archandra Lameere, 1912, stat. nov. and Acutandra gen. nov. The genus Parandra is subdivided in two subgenera: Parandra (Parandra) s. str. and Parandra (Birandra) subgen. nov. The geographical distribution of P. (P.) laevis Latreille, 1804 is commented and the probable synonymy between P. cubaecola Chevrolat, 1862 and P. (P.) cribrata Thomson, 1861 is discussed. New species described: P. (P.) tavakiliani from Puerto Rico and P. (Birandra) mariahelenae from Jamaica. New combinations: Neandra brunnea (Fabricius, 1798), Neandra marginicollis (Schaeffer, 1929), Archandra caspia (Ménétriès, 1832), Acutandra punctatissima (Thomson, 1861), A. degeeri (Thomson, 1867), A. murrayi (Lameere, 1912), A. araucana (Bosq, 1951), A. ubitiara (Santos-Silva & Martins, 2000), all from Parandra. Keys to genera of Parandrini, subgenera of Parandra and American species of Parandra and Acutandra are added.

Morphological development of Corydoras aff. paleatus (Siluriformes, Callichthyidae) and correlation with the emergence of motor and social behaviors

Here we examine major anatomical characteristics of Corydoras aff. paleatus (Jenyns, 1842) post-hatching development, in parallel with its neurobehavioral evolution. Eleutheroembryonic phase, 4.3-8.8 days post-fertilization (dpf); 4.3-6.4 mm standard length (SL) encompasses from hatching to transition to exogenous feeding. Protopterygiolarval phase (8.9-10.9 dpf; 6.5-6.7 mm SL) goes from feeding transition to the commencement of unpaired fin differentiation, which marks the start of pterygiolarval phase (11-33 dpf; 6.8-10.7 mm SL) defined by appearance of lepidotrichia in the dorsal part of the median finfold. This phase ends with the full detachment and differentiation of unpaired fins, events signaling the commencement of the juvenile period (34-60 dpf; 10.8-18.0 mm SL). Eleutheroembryonic phase focuses on hiding and differentiation of mechanosensory, chemosensory and central neural systems, crucial for supplying the larval period with efficient escape and nutrient detection-capture neurocircuits. Protopterygiolarval priorities include visual development and respiratory, digestive and hydrodynamic efficiencies. Pterygiolarval priorities change towards higher swimming efficacy, including carangiform and vertical swimming, necessary for the high social interaction typical of this species. At the end of the protopterygiolarval phase, simple resting and foraging aggregations are seen. Resting and foraging shoals grow in complexity and participant number during pterygiolarval phase, but particularly during juvenile period.