Nitrate reductase activity and spad readings in leaf tissues of guinea grass submitted to nitrogen and potassium rates

Nitrogen and K deficiency are among the most yield limiting factors in Brazilian pastures. The lack of these nutrients can hamper the chlorophyll biosynthesis and N content in plant tissues. A greenhouse experiment was carried out to evaluate the relationship among N and K concentrations, the indirect determination of chlorophyll content (SPAD readings), nitrate reductase activity (RNO3-) in newly expanded leaf lamina (NL) and the dry matter yield for plant tops of Mombaça grass (Panicum maximum Jacq.). A fractionated 5² factorial design was used, with 13 combinations of N and K rates in the nutrient solution. The experimental units were arranged in a randomized block design, with four replications. Plants were harvested twice. The first harvest occurred 36 days after seedling transplanting and the second 29 days after the first. Significance occurred for the interaction between the N and K rates to SPAD readings and to RNO3- assessment taken on the NL during the first growth. Besides, RNO3- and SPAD readings increased only with the NL N concentration, reaching the highest values of both variables up to about 25 g kg-1, but were ratively constant at higher leaf N. Significant relationships either between SPAD readings or RNO3- activity and shoot dry mass weight were also observed. The critical levels of N concentration in the NL were, respectively, 22 and 17g kg-1 in the first and second harvest. Thus, SPAD instrument and RNO3- assessment can be used as complementary tools to evaluate the N status in forage grass.

Dry matter production of Tanzania grass as a function of agrometeorological variables

The objective of this work was to develop and validate linear regression models to estimate the production of dry matter by Tanzania grass (Megathyrsus maximus, cultivar Tanzania) as a function of agrometeorological variables. For this purpose, data on the growth of this forage grass from 2000 to 2005, under dry‑field conditions in São Carlos, SP, Brazil, were correlated to the following climatic parameters: minimum and mean temperatures, degree‑days, and potential and actual evapotranspiration. Simple linear regressions were performed between agrometeorological variables (independent) and the dry matter accumulation rate (dependent). The estimates were validated with independent data obtained in São Carlos and Piracicaba, SP, Brazil. The best statistical results in the development and validation of the models were obtained with the agrometeorological parameters that consider thermal and water availability effects together, such as actual evapotranspiration, accumulation of degree‑days corrected by water availability, and the climatic growth index, based on average temperature, solar radiation, and water availability. These variables can be used in simulations and models to predict the production of Tanzania grass.

Selection of full-sib families of Panicum maximum Jacq under low light conditions

The silvopastoral system is a viable technological alternative to extensive cattle grazing, however, for it to be successful, forage grass genotypes adapted to reduced light need to be identified. The objective of this study was to select progenies of Panicum maximum tolerant to low light conditions for use in breeding programs and to study the genetic control and performance of some traits associated with shade tolerance. Six full-sib progenies were evaluated in full sun, 50% and 70% of light reduction in pots and subjected to cuttings. Progeny genotypic values ​​(GV) increased with light reduction in relation to plant height (H) and specific leaf area (SLA). The traits total dry mass accumulation (DM) and leaf dry mass accumulation (LDM) had GV higher in 50% shade and intermediate in 70% shade. The GV of tiller number (TIL) and root dry mass accumulation (RDM) decreased with light reduction. The highest positive correlations were obtained for the traits H and RDM with SLA and DM; the highest negative correlations were between TIL and SLA and RDM, and H and LDM. The progenies showed higher tolerance to 50% light reduction and, among them, two stood out and will be used in breeding programs. It was also found that it is not necessary to evaluate some traits under all light conditions. All traits had high broad sense heritability and high genotypic correlation between progenies in all light intensities. There is genetic difference among the progenies regarding the response to different light intensities, which will allow selection for shade tolerance

BRACHIARIA IN SELENIUM-CONTAMINATED SOIL UNDER SULPHUR SOURCE APPLICATIONS

ABSTRACT High contents of plant-available selenium in the soil in the form of selenate, resulting from natural or anthropogenic action, jeopardizes agricultural areas and requires research for solutions to establish or re-establish agricultural or livestock operation, avoiding the risk of poisoning of plants, animals and humans. The purpose was to evaluate sulfur sources in the form of sulfate, e.g., ammonium sulfate, calcium sulfate, ferric sulfate, in the remediation of tropical soils anthropogenically contaminated with Se under the tropical forage grass Brachiaria brizantha (Hochst. ex A. Rich.) Stapf cv. Marandu. More clayey soils are less able to supply plants with Se, which influences the effects of S sources, but it was found that high soil Se concentrations negatively affected forage biomass production, regardless of the soil. Of the tested S sources, the highly soluble ammonium sulfate and ferric sulfate reduced plant Se uptake and raised the available sulfur content in the soil.

Diurnal variations of non-structural carbohydrates in vegetative tissues of Melinis minutiflora, Echinolaena inflexa and Lolium multiflorum (Poaceae)

Echinolaena inflexa (Poir.) Chase is a native C3 grass species with high biomass production and abundance in the cerrado comparable to Melinis minutiflora Beauv., a C4 forage grass of African origin, widespread in the cerrado, displacing some native herbaceous species. These species accumulate mainly starch, differently of Lolium multiflorum Lam. ssp. italicum Beck cv. Lema (ryegrass), a C3 temperate forage grass that accumulates fructans as the major storage carbohydrate in the vegetative tissues. In the present study, we analyzed diurnal variations in the content and composition of non-structural carbohydrates (NSC) in the aerial vegetative parts of the three species. Results showed similarity in water-soluble carbohydrates (WSC) and starch contents between Melinis minutiflora and Echinolaena inflexa, with the exotic grass accumulating higher amounts of NSC. However, the levels of sucrose, glucose and fructose were 10 fold higher in Lolium multiflorum, that accumulates fructan as the main storage carbohydrate. Although the contents of WSC and starch were similar in the tropical species E. inflexa and M. minutiflora, their diurnal variations were different and could be related to starch metabolism, especially in M. minutiflora.

Symmetric pollen mitosis I and suppression of pollen mitosis II prevent pollen development in Brachiaria jubata (Gramineae)

Microsporogenesis and pollen development were analyzed in a tetraploid (2n = 4x = 36) accession of the forage grass Brachiaria jubata (BRA 007820) from the Embrapa Beef Cattle Brachiaria collection that showed partial male sterility. Microsporocytes and pollen grains were prepared by squashing and staining with 0.5% propionic carmine. The meiotic process was typical of polyploids, with precocious chromosome migration to the poles and laggards in both meiosis I and II, resulting in tetrads with micronuclei in some microspores. After callose dissolution, microspores were released into the anther locule and appeared to be normal. Although each microspore initiated its differentiation into a pollen grain, in 11.1% of them nucleus polarization was not observed, i.e., pollen mitosis I was symmetric and the typical hemispherical cell plate was not detected. After a central cytokinesis, two equal-sized cells showing equal chromatin condensation and the same nuclear shape and size were formed. Generative cells and vegetative cells could not be distinguished. These cells did not undergo the second pollen mitosis and after completion of pollen wall synthesis each gave rise to a sterile and uninucleate pollen grain. The frequency of abnormal pollen mitosis varied among flowers and also among inflorescences. All plants were equally affected. The absence of fertile sperm cells in a considerable amount of pollen grains in this accession of B. jubata may compromise its use in breeding and could explain, at least in part, why seed production is low when compared with the amount of flowers per raceme.

Sulphuric acid scarification effects on Brachiaria brizantha, B. humidicola and Panicum maximum seed dormancy release

Most tropical forage grass species have dormant seeds, which reduce percentages in germination tests. The objective of this study was to evaluate H2SO4 scarification effects on seed dormancy releasing, through germination time (T50) and variability among germination test replicates, in 630, 94 and 82 seed samples of B. brizantha, B. humidicola and P. maximum, respectively, tested at the Central Seed Testing Laboratory, Campinas, Brazil, from 1991 to 1999. Germination tests used two 4 x 100 replicates of intact and scarified seeds (15-, 10-, 5-minute treatments, respectively). Mean germination time (T50) and variability among germination replicates were also analysed. Statistical analysis was performed by t-test paired samples for means. Scarification promoted general decreases in T50, while variability among germination test replicates was reduced in B. brizantha. Scarification increase germination in B. brizantha and P. maximum, but is deleterious in B. humidicola.

Tillering and forage accumulation in Marandu grass under different grazing intensities

Studies of plant responses to defoliation are important to develop pasture management strategies. The objective of this study was to evaluate the population density of basal, aerial and reproductive tillers, tiller appearance and mortality rates, forage accumulation and sward structure in Marandu grass pastures under different grazing intensities. The experimental period was from January to June 2006, divided in three seasons: summer, autumn and winter. The pastures were continuously grazed using variable stocking rates. The grazing intensities corresponded to 15, 30 and 45 cm of sward height. The experiment was arranged in a complete randomized block design with three treatments and two replicates. The sward heights were measured twice a week. The response variables were: forage accumulation, forage mass and its morphological components; and population densities of basal (TPDb), aerial (TPDa) and reproductive (TPDr) tillers. The highest TPDb (P > 0.05) was recorded for the shortest sward pasture, and the highest TPDr (P<0.05) for the tallest sward pasture. Swards showed a tiller size/density compensation mechanism and, consequently, the forage accumulation was similar (P > 0.05) among the grazing intensities. Pasture with a sward height of 35 cm had 94% of sun light interception. The highest variations in forage accumulation and sward structure were more influenced by seasonal differences than by grazing intensities. Pastures of Marandu grass showed large flexibility in grazing management, which allowed it to be maintained at sward heights between 15 and 35 cm.

Differential vegetative and reproductive performances among fifteen guinea grass hybrids

The main scope of this work was to detect (Panicum maximum Jacq.) genotype differences as to morphoagronomic and seed quality indices, and to establish character correlations useful for determining vegetative and reproductive trends. Besides the flowering cycle, eight phenological and two seed quality traits were scored in a greenhouse randomized complete block experiment, as follows: plant height (PH), reproductive tiller number/overall tiller number (RTN/OTN), panicle number/reproductive tillers (PN/RT), leaf length (LL), leaf width (LW), panicle length (PL), fresh weight (FW), dry weight (DW), number of seeds/g (NS/G) and seed sample physical purity (SPP). Very-early and early-flowering hybrids consistently showed the highest correlation values among flowering cycle and RTN/OTN (r = -0.59**), PN/RT (r = -0.48**), NS/G (r = -0.88**) and SPP (r = -0.80**) (reproductive parameters) while intermediate and late-flowering hybrids presented the highest values for LL (r = 0.53**), LW (r = 0.60**), PL (r = 0.77**), FW (r = 0.78**) and DW (r = 0.85**) (vegetative traits). The implications of these results for plant breeding and forage management purposes are discussed.

Morphogenetic characteristics and management of Tanzania grass

The objective of this work was to evaluate the effect of grazing interval and period of evaluation over tissue turnover in Tanzania grass pastures (Panicum maximum cv. Tanzania) and to ascertain if herbage accumulation rate can be used as a criterion to establish a defoliation schedule for this grass in Southeast of Brazil. A randomized block design with a split-plot arrangement was used. The effect of three grazing intervals was evaluated within seven periods between October 1995 and September 1996. Responses monitored were leaf and stem elongation rates, leaf senescence rate, stem length, and tiller density. Net herbage accumulation rate was calculated using tissue turnover data. The grazing intervals for Tanzania grass should be around 38 days between October and April (spring and early autumn) and 28 days during the reproductive phase of the grass (April/May). Between May and September (late autumn and winter), grazing interval should be around 48 days. Herbage accumulation rate is not a good criterion to establish defoliation time for Tanzania grass. Studies on the effects of stem production in grazing efficiency, animal intake and forage quality are needed to improve Tanzania grass management.

Forage yield and quality of a dense thorny and thornless "jurema-preta" stand

The objective of this work was to compare forage production and quality of thorny and thornless "jurema-preta" (Mimosa tenuiflora (Willd.) Poiret) in a dense planted stand, subjected to annual pruning of fine branches, in Patos, PB, Brazil. The experiment consisted of two treatments (thornless and thorny "jurema-preta") in a complete randomized block design, with ten replicates of two linear plots subdivided in time. Forage mass and chemical composition of fine branches and the basal diameter of plants were measured during five years. Pruning decreased (p<0.05) increments in basal diameter and forage production. Annual dry matter yields reached 4,108 and 5,833 kg ha-1, respectively, for thornless and thorny plants, and forage quality was similar (p>0.05) for both genotypes. This roughage fodder (minimum NDF and ADF averages were 56±1.1% and 43±1.0%, respectively) had low P and K concentrations. Its average crude protein content was greater than 9.9±0.5%, which exceeds the minimum necessary for animal maintenance. Both "jurema-preta" genotypes tolerated pruning of fine branches and contributed with a significant amount of roughage fodder for animal maintenance in the dry season.

Forage production of elephant grass under intermittent stocking

The objective of this work was to evaluate the dry matter production of elephant grass (Pennisetum purpureum) genotypes, managed under intermittent stocking. A completely randomized design was used, with two genotypes and three replicates. The treatments consisted of factorial combinations (2x2x2) of genotypes ('BRS Kurumi' and the clone CNPGL 00‑1‑3), two light interception levels (LI) at the onset of grazing (90 and 95%), and two post‑grazing canopy heights (30 and 50 cm). A total of 24 Holstein x Zebu crossbred heifers were used. The stocking density varied in order to finish the grazing periods in two days. The interval between the defoliation, based on 95% LI, resulted in a higher leaf mass per grazing cycle. The post‑grazing height of 30 cm did not affect the number of grazing cycles but provided a greater herbage accumulation rate. The cultivar BRS Kurumi has higher pasture growth, lower rest period, and greater number of grazing cycles, which results in increased forage production in the growing season.

Forage potential and silage quality of four varieties of saccharine sorghum

Abstract: The objective of this work was to evaluate four cultivars of saccharine sorghum (Sorghum bicolor) regarding productivity, chemical composition of plant parts, and quality of the ensiling process. The tested varieties of saccharine sorghum were BRS 506, BRS 508, BRS 509, and BRS 511. The experiment was divided into two trials, which assessed: production, morphological composition, and nutritional quality of the saccharine varieties; and fermentation quality and nutritional value of the silage produced from the saccharine varieties. Of the tested varieties, BRS 509 and BRS 511 showed the highest total dry matter (DM) production. The BRS 508 variety presented the highest in vitro digestibility of the whole plant (70.65% DM). During ensiling, the BRS 509 variety showed the lowest DM loss (8.87%). The highest effluent production was observed for BRS 506 and BRS 508, with yields of 521.87 and 393.16 kg Mg-1 ensiled DM, respectively. The BRS 511 variety is the most recommended because of the best results for plant production and nutritional quality. Regarding the ensiling process, BRS 509 presents the lowest fermentation losses and the highest nutritional value of silage.

Effect of the application of different water depths and nitrogen and potassium doses on quality of Tanzania grass

The objective of this study was to evaluate the effects of the application of different water depths and nitrogen and potassium doses in the quality of Tanzania grass, in the southern of the state of Tocantins. The experiment was conducted on strips of traditional sprinklers, and used, as treatments, a mixture of fertilizer combinations of N and K2O always in the ratio of 1 N:0.8 K2O. This study determined throughout the experiment: plant height (PH), the crude protein (CP) and neutral detergent fiber (NDF). The highest plant height obtained was 132.4 cm, with a fertilizer dose of 691.71 kg ha-1 in the proportion of N:0.8 K2O, in other words, 384.28 kg ha-1 of N and 307.43 kg ha-1 of K2O, and water depth of 80% of the ETc. The highest crude protein content was 12.2%, with the fertilizer dose application of 700 kg ha-1 yr-1 in the proportion of 1 N to 0.8 of K2O, in other words, 388.89 kg ha-1 of N and 311.11 kg ha-1 of K2O and absence of irrigation. The lowest level of neutral detergent fiber was 60.7% with the application of the smallest dose of fertilizer and highest water depth. It was concluded in this study that there was an increase in plant height by increasing the fertilizer dose and water depth. The crude protein content increased 5.4% in the dry season, by increasing the fertilizer dose and water depth. In the dry season, there was an increase of NDF content by 4.5% by increasing the application of fertilizer and water depth.

Yield and quality of elephant grass biomass produced in the cerrados region for bioenergy

The objective of this study was to evaluate the performance of two genotypes of elephant grass, fertilized with and without N, for biomass production for energy use under the edaphoclimatic conditions of the Cerrado. The genotypes Roxo and Paraíso, grown in a field experiment in a Latosol in the Cerrado region were evaluated for biomass yield, nitrogen accumulation, C:N and stem:leaf ratios, fibre, ash and P and K contents and calorific value. The accumulated dry biomass ranged from 30 to 42 Mg ha-1 and showed no response to nitrogen fertilization with the lowest biomass obtained by the genotype Paraíso and the highest by Roxo. The total N accumulation followed the same pattern as for dry matter, ranging from 347 to 539 kg N ha-1. C:N and stem:leaf ratio of the biomass produced did not vary with treatments. The fibre contents were higher in genotype Paraíso and the highest levels of ash in the genotype Roxo. The K content in the biomass was higher in genotype Roxo and P did not vary between genotypes. The calorific value averaged 18 MJ kg-1 of dry matter and did not vary with the levels of N in leaves and stems of the plant. Both genotypes, independent of N fertilization, produced over 30 Mg ha-1 of biomass under Cerrado conditions.