Promoção da informação sobre tecnologias e produtos orgânicos na Embrapa Hortaliças

Pesquisa descritiva, de caráter exploratório, com o objetivo de verificar como a informação sobre tecnologias e produtos orgânicos, divulgada pela Embrapa Hortaliças, é utilizada pelos produtores orgânicos de hortaliças do Distrito Federal. O estudo teve como universo os empregados da Embrapa Hortaliças envolvidos com o processo de divulgação dessa informação e os produtores orgânicos de hortaliças certificados do Distrito Federal. A coleta de dados foi realizada por meio da análise de documentos da empresa e do levantamento por entrevistas semiestruturadas realizadas com dois pesquisadores atuantes no Projeto Implantação do Centro de Desenvolvimento Tecnológico da Agricultura do Distrito Federal (CDTORG), nove funcionários da Área de Comunicação e Negócios (ACN) da Embrapa Hortaliças e com 18 produtores orgânicos de hortaliças certificados do Distrito Federal. As entrevistas foram transcritas e analisadas de acordo com a técnica de análise de conteúdo. Foi observado que o processo de divulgação da informação sobre tecnologias e produtos orgânicos realizado pela empresa não possui foco no público receptor dessa informação, uma vez que não eram realizados estudos para identificar suas necessidades e preferências. Verificou-se que os produtores orgânicos de hortaliças eram indivíduos experientes, bem informados e que estavam cientes de muitos problemas do processo de divulgação estudado.

Simultaneous determination of moxifloxacin and H2 receptor antagonist in pharmaceutical dosage formulations by RP-HPLC: application to in vitro drug interactions

Simultaneous determination of moxifloxacin (MOX) and H2-antagonists was first time developed in bulk and formulations. Purospher STAR C18 (250 x 4.6 mm, 5 μm) column was used. The mobile phase (methanol: water: ACN, 60:45:5 v/v/v, pH 2.7) was delivered at a flow rate of 1.0 mL min-1, eluent was monitored at 236, 270 and 310 nm for cimetidine, famotidine and ranitidine, respectively. The proposed method is specific, accurate (98-103%), precise (intra-day and inter-day variation 0.098-1.970%) and linear (r>0.998). The LOD and LOQ were 0.006-0.018 and 0.019-0.005 μg mL-1, respectively. The statistical parameters were applied to verify the results. The method is applicable to routine analysis of formulations and interaction of MOX with H2-antagonist.

CROMATOGRAFIA POR INTERAÇÕES HIDROFILICAS (HILIC): ESTADO DA ARTE E APLICAÇÕES

Hydrophilic interaction liquid chromatography (HILIC) has been gaining increased attention for its effective separation of highly polar compounds, which include carbohydrates, amino acids, pharmaceutical compounds, proteins, glycoproteins, nucleosides, etc. Polar compounds are usually poorly retained on reverse-phase liquid chromatography (RP-HPLC) columns or have poor solubility in the apolar mobile phase of normal-phase high performance liquid chromatography (NP-HPLC). Since HILIC uses organic solvents such as ACN or MeOH ( > 70%), also used in RP-HPLC and polar stationary phases similar to NP-HPLC (bare silica, diol, amino, amide, saccharide, zwitterionic stationary phases, etc.), it represents a hybrid of the two separation modes. The high organic content in the MP leads to good compatibility with mass spectrometry (MS), increasing the detectivity. This review describes the fundamentals of HILIC and highlights some interesting applications.

Thermodynamic quantities of solvation and dilution for some acetanilide derivatives in octanol and water mutually saturateds

Based on published thermodynamic quantities for solution, partitioning and sublimation of acetanilide (ACN), acetaminophen (ACP) and Phenacetin (PNC), the thermodynamic quantities for drugs solvation in octanol-saturated water (W(ROH)) and water-saturated octanol (ROH(W)) as well as the drugs dilution in ROH(W) were calculated. The Gibbs energies of solvation were favourable in all cases. The respective enthalpies and entropies were negative indicating an enthalpy-driving for the solvation process in all cases. On the other hand, the Gibbs energies of dilution were favourable for ACP and PNC but unfavourable for ACN, whereas the respective enthalpies and entropies were negative for ACP and PNC but positive for ACN indicating enthalpy-driving for the dilution process in the case of the former drugs and entropy-driving for the latter. From the obtained values for the transfer processes, an interpretation based on solute-solvent interactions was developed.