Aspectos analíticos e regulatórios na determinação de resíduos de macrolídeos em alimentos de origem animal por cromatografia líquida associada à espectrometria de massas

This is an overview of LC-MS techniques applied for macrolide determination in food, including sample preparation and method validation, as well as the policies adopted by international agencies regarding their presence in food. Techniques for the analysis of macrolides in food normally include solid phase or liquid-liquid extraction followed by HPLC. UHPLC presents advantages in running time, detectability and solvent consumption. Triple-quadrupoles are the most common analyzers in instruments used for the determination of contaminants in food, but time-of-flight and ion-trap spectrometers have been successfully applied for analyses focusing on the investigation of structural formula or the presence of degradation products.

O estado da arte na determinação de resíduos de medicamentos veterinários em alimentos de origem animal empregando técnicas cromatográficas acopladas à espectrometria de massas

The determination of veterinary drug residues in foods of animal origin is an important issue because of the risk these compounds pose to human health in addition to their persistence and tendency to bioaccumulate. In recent years, significant progress has been made in the area and this review presents the state of the art in sample preparation procedures associated with chromatographic techniques coupled to mass spectrometry for multiresidue determination of veterinary drugs in food of animal origin at concentration levels suitable for the control of residues and contaminants in food.

OMNIS HOMO DE NECESSITATE EST ANIMAL: SIGNIFICACIÓN Y REFERENCIA VACÍA EN LA SEGUNDA MITAD DEL SIGLO 13

A questão "se uma elocução (palavra/termo/nome) perde seu significado com a destruição das coisas (quer dizer, as coisas significadas)"surge como uma questão sobre o valor-verdade de declarações com um termo vazio como sujeito, a saber, como um subproblema do sofisma "Se 'omnis homo de necessitate est animal' é verdade quando não há homem algum (=OHNEA)". Neste trabalho, trarei as discussões conforme elas se apresentam em "De signis" IV.2 de Roger Bacon, em "Quaestiones logicales", q. 2–3 de Peter John Olivi, no OHNEA de Boethius of Dacia, e no OHNEA de Anonymus Alani. Tais textos nos apresentam quatro modos diversos de nos relacionarmos com a questão sobre significação para OHNEA e, assim, com quatro posições diferentes sobre a relação entre significação e referência vazia. Usarei Anonymus Alani como o fio conductor de minha análise, inserindo outras posições onde tal se fizer relevante.

Zoneamento bioclimático da região sudeste do Brasil para o conforto térmico animal e humano

O objetivo deste trabalho foi avaliar as condições de clima da região Sudeste do Brasil, por meio do índice de temperatura e umidade (ITU), e apresentar o zoneamento bioclimático relacionado ao conforto térmico animal e humano. Os valores de ITU foram estimados a partir de dados de temperatura e umidade relativa do ar para o período de 1980 a 2000. Quatro intervalos de ITU foram usados para classificar o desempenho humano (ITU < 74: conforto; 74 < ITU < 79: quente; 79 < ITU < 84: muito quente, e ITU > 84: extremamente quente), e dois intervalos para classificar a produção animal (79 < ITU < 84: perigoso e ITU > 84: emergência). Os resultados mostraram maior risco de desconforto térmico no período de outubro a abril, comparado ao período entre maio e setembro. O período mais crítico ocorreu entre dezembro e março. Apesar de se ter observado que valores de ITUméd entre 79 e 84 ocorreram em menos de 3,6% do tempo, humanos, bem como animais, podem sofrer algum grau de estresse térmico durante as horas mais quentes do dia, afetando ambos negativamente, produção animal e rendimento humano. Valores de ITU maiores que 84 não foram observados para a região estudada.

Produção animal e o meio ambiente: uma comparação entre potencial de emissão de metano dos dejetos e a quantidade de alimento produzido

O objetivo do trabalho foi efetuar a comparação das principais espécies de interesse agropecuário, em relação à eficiência de conversão das dietas em produtos de origem animal (carne ou ovo), produção de resíduo e potencial de emissão de metano, a partir da fermentação dos resíduos. Para isso, foram selecionadas cinco espécies de animais durante a fase de produção: 1) suínos, do nascimento ao abate (peso vivo (PV) final de 90 kg) ; 2) bovinos, do desmame ao abate (PV: 520 kg); 3) caprinos, do desmame ao abate (PV: 30 kg); 4) aves, durante toda a fase de postura (14,7 kg de ovos); 5) frango de corte, do nascimento ao abate (PV: 3,1 kg). Para a estimativa dos parâmetros propostos, foram medidos os dados do desempenho e efetuou-se a biodigestão anaeróbia dos dejetos produzidos pelos animais. De maneira geral, os dejetos dos ruminantes apresentaram altas concentrações de fibra e baixos potenciais de produção de biogás; no entanto, o menor desempenho destes animais na conversão do alimento em produto e a maior produção de dejetos fizeram com que eles apresentassem maiores produções de metano por kg de alimento produzido.

Method of numerical correction of errors occasioned by delay of records during the monitoring of environmental variables of interest for animal production

Thermal and air conditions inside animal facilities change during the day due to the influence of the external environment. For statistical and geostatistical analyses to be representative, a large number of points spatially distributed in the facility area must be monitored. This work suggests that the time variation of environmental variables of interest for animal production, monitored within animal facility, can be modeled accurately from discrete-time records. The aim of this study was to develop a numerical method to correct the temporal variations of these environmental variables, transforming the data so that such observations are independent of the time spent during the measurement. The proposed method approached values recorded with time delays to those expected at the exact moment of interest, if the data were measured simultaneously at the moment at all points distributed spatially. The correction model for numerical environmental variables was validated for environmental air temperature parameter, and the values corrected by the method did not differ by Tukey's test at 5% significance of real values recorded by data loggers.

Evaluation of roof slope and exposure with different roofing materials in reduced models of animal production facilities in spring and summer

The present study was conducted at the Department of Rural Engineering and the Department of Animal Morphology and Physiology of FCAV/Unesp, Jaboticabal, SP, Brazil. The objective was to verify the influence of roof slope, exposure and roofing material on the internal temperature of reduced models of animal production facilities. For the development of the research, 48 reduced and dissemble models with dimensions 1.00 × 1.00 × 0.50 m were used. The roof was shed-type, and the models faced to the North or South directions, with 24 models for each side of exposure. Ceramic, galvanized-steel and fibro tiles were used to build the roofs. Slopes varied between 20, 30, 40 and 50% for the ceramic tile and 10, 30, 40 and 50% for the other two. Inside the models, temperature readings were performed at every hour, for 12 months. The results were evaluated in a general linear model in a nested 3 × 4 × 2 factorial arrangement, in which the effects of roofing material and exposure were nested on the factor Slope. Means were compared by the Tukey test at 5% of probability. After analyzing the data, we observed that with the increase in the slope and exposure to the South, there was a drop in the internal temperature within the model at the geographic coordinates of Jaboticabal city (SP/Brazil).

Ultrasound evaluation on carpal tunnel syndrome before and after bariatric surgery

Objective:To evaluate the prevalence of carpal tunnel syndrome in candidates for bariatric surgery comparing with the non-obese population and verify the effects on it of bariatric treatment. Methods:We studiedthree groups of individuals: 1) patients waiting for bariatric surgery (preoperative); 2) individuals who had already undergone the procedure (postoperative); and 3) control group. We collected demographic and clinical data of carpal tunnel syndrome. The Ultrasound examination was carried out to diagnose the syndrome by measuring the median nerve area. Results:We included 329 individuals (114 in the preoperative group, 90 in the postoperative group and 125 controls). There was a higher prevalence of paresthesias (p=0.0003), clinical tests (p=0.0083) on the preoperative group when compared with controls (p<0.00001). There were lowe levels of paresthesias (p=0.0002) and median nerve area (p=0.04) in postoperative patients but with no significant difference in general. A significant difference was found between the preoperative and postoperative groups (p=0.05) in those who performed non-manual work.Conclusion: There was a higher prevalence of carpal tunnel syndrome in the preoperative group compared with the control one, but no significant difference was observed between the pre and postoperative groups in general. There was difference between pre and postoperative groups for non-manual workers.

Assessment of pelvic floor by three-dimensional-ultrasound in primiparous women according to delivery mode: initial experience from a single reference service in Brazil

PURPOSE: To evaluate changes to the pelvic floor of primiparous women with different delivery modes, using three-dimensional ultrasound. METHODS: A prospective cross-sectional study on 35 primiparae divided into groups according to the delivery mode: elective cesarean delivery (n=10), vaginal delivery (n=16), and forceps delivery (n=9). Three-dimensional ultrasound on the pelvic floor was performed on the second postpartum day with the patient in a resting position. A convex volumetric transducer (RAB4-8L) was used, in contact with the large labia, with the patient in the gynecological position. Biometric measurements of the urogenital hiatus were taken in the axial plane on images in the rendering mode, in order to assess the area, anteroposterior and transverse diameters, average thickness, and avulsion of the levator ani muscle. Differences between groups were evaluated by determining the mean differences and their respective 95% confidence intervals. The proportions of levator ani muscle avulsion were compared between elective cesarean section and vaginal birth using Fisher's exact test. RESULTS: The mean areas of the urogenital hiatus in the cases of vaginal and forceps deliveries were 17.0 and 20.1 cm², respectively, versus 12.4 cm² in the Control Group (elective cesarean). Avulsion of the levator ani muscle was observed in women who underwent vaginal delivery (3/25), however there was no statistically significant difference between cesarean section and vaginal delivery groups (p=0.5). CONCLUSION: Transperineal three-dimensional ultrasound was useful for assessing the pelvic floor of primiparous women, by allowing pelvic morphological changes to be differentiated according to the delivery mode.

Changes in motor behavior during pregnancy in rats: the basis for a possible animal model of restless legs syndrome

PURPOSE:Pregnant women have a 2-3 fold higher probability of developing restless legs syndrome (RLS – sleep-related movement disorders) than general population. This study aims to evaluate the behavior and locomotion of rats during pregnancy in order to verify if part of these animals exhibit some RLS-like features.METHODS:We used 14 female 80-day-old Wistar rats that weighed between 200 and 250 g. The rats were distributed into control (CTRL) and pregnant (PN) groups. After a baseline evaluation of their behavior and locomotor activity in an open-field environment, the PN group was inducted into pregnancy, and their behavior and locomotor activity were evaluated on days 3, 10 and 19 of pregnancy and in the post-lactation period in parallel with the CTRL group. The serum iron and transferrin levels in the CTRL and PN groups were analyzed in blood collected after euthanasia by decapitation.RESULTS:There were no significant differences in the total ambulation, grooming events, fecal boli or urine pools between the CTRL and PN groups. However, the PN group exhibited fewer rearing events, increased grooming time and reduced immobilization time than the CTRL group (ANOVA, p<0.05).CONCLUSION:These results suggest that pregnant rats show behavioral and locomotor alterations similar to those observed in animal models of RLS, demonstrating to be a possible animal model of this sleep disorder.