Benthic macroinvertebrates as bioindicators of water quality in an Atlantic forest fragment

The objective of this study was to evaluate benthic macroinvertebrate communities as bioindicators of water quality in five streams located in the "Reserva Particular do Patrimônio Natural" (RPPN) Mata Samuel de Paula and its surroundings, in the municipality of Nova Lima near the city of Belo Horizonte, Minas Gerais State, southeastern Brazil. This region has been strongly modified by human activities including mining and urbanization. Samples were collected in the field every three months between August 2004 and November 2005, totaling six samplings in the rainy and dry seasons. This assessment identified one area ecologically altered while the other sampling sites were found to be minimally disturbed systems, with well-preserved ecological conditions. However, according to the Biological Monitoring Work Party (BMWP) and the Average Score Per Taxon (ASPT) indices, all sampling sites had excellent water quality. A total of 14,952 organisms was collected, belonging to 155 taxa (148 Insecta, two Annelida, one Bivalvia, one Decapoda, one Planariidae, one Hydracarina, and one Entognatha). The most abundant benthic groups were Chironomidae (47.9%), Simuliidae (12.3%), Bivalvia (7.5%), Decapoda (6.1%), Oligochaeta (5.2%), Polycentropodidae (3.7%), Hydropsychidae (2.5%), Calamoceratidae (1.8%), Ceratopogonidae (1.7%), and Libellulidae (1.2%). The assessment of the benthic functional feeding groups showed that 34% of the macroinvertebrates were collector-gatherers, 29% predators, 24% collector-filterers, 8% shredders, and 5% scrapers. The RPPN Mata Samuel de Paula comprises diversified freshwater habitats that are of great importance for the conservation of many benthic taxa that are intolerant to organic pollution.

Cytogenetic study in natural hybrids of Callithrix (Callitrichidae: Primates) in the Atlantic forest of the state of Rio de Janeiro, Brazil

In the Atlantic forest of Rio de Janeiro, Callithrix aurita (É. Geoffroy in Humboldt, 1812) is a native species vulnerable to extinction and C. jacchus (Linnaeus, 1758) and C. penicillata (É. Geoffroy, 1812) are invasive species. The major threats to the native species are habitat degradation and hybridization, although there are currently no genetic data about natural hybrids available. Previous studies have revealed that species of the Callithrix genus are extremely homogeneous in their karyotypes with the exceptions of the morphology and size of the Y chromosome and its nucleolar organizer region (NOR) banding pattern. Three male marmosets captured in the wild in Guapimirim municipality, Rio de Janeiro, Brazil, considered as possible hybrids between C. aurita and C. jacchus or C. penicillata on the basis of pelage pattern, were cytogenetically studied. Metaphase chromosomes were obtained by using short-term lymphocyte cultures and Ag-NOR staining was performed. The hybrids karyotypes were 2n=46, 14 uni- and 30 bi-armed autosomes, a median size submetacentric X and NOR bearing autosomes, being compatible with that observed for the genus. In the three individuals studied, Y chromosomes were similar to those found for C. aurita, without NORs. The data obtained suggest the involvement of C. aurita in natural hybridization with one of the invasive species. We discuss the possible consequences of this hybridization.

Use of camera-traps in natural trails and shelters for the mammalian survey in the Atlantic Forest

In order to evaluate the efficiency of different mammalian survey methods, we compared traditional sampling techniques (use of camera-traps on roads and artificial trails, track censuses, and direct field visualization) with an alternative sampling design (camera-traps positioned in natural areas such as natural trails and shelters). We conducted the study in a deciduous Atlantic-Forest park in southern Brazil, and additionally compared our results with a previous intensive study carried out in the same area. Our considerably smaller sampling effort (example: 336 trap.day for our camera-traps versus 2,154 trap.day for the earlier study) registered the presence of 85% of the local known species, with camera-traps being 68% efficient. Moreover, shelter camera-traps revealed a different species composition regarding most of other sampling methods. This sampling strategy involving natural forest sites was therefore able to effectively optimize the chances of evaluating species composition in a shorter period, especially with respect to lower-density and cryptic species, as well as to detect species that avoid open, disturbed sites such as roads and man-made forest trails.

Bird community in an Araucaria forest fragment in relation to changes in the surrounding landscape in Southern Brazil

The dynamics of the bird community in a small forest fragment was evaluated along seven years in relation to changes in the surrounding landscape. The study area is an Araucaria forest fragment in Southern Brazil (state of Paraná). The sampling period covered the years 1988 through 1994 and the mark-release-recapture method was utilized. The landscape analysis was based on Landsat TM images, and changes in exotic tree plantations, native forest, open areas (agriculture, pasture, bare soil, and abandoned field), and "capoeira"(native vegetation < 2 m) were quantified. The relationship between landscape changes and changes in abundance diversity of forest birds, open-area birds, forest-edge birds, and bamboo specialists was evaluated. Richness estimates were run for each year studied. The richness recorded in the study area comprised 96 species. The richness estimates were 114, 118 and 110 species for Chao 1, Jackknife 1 and Bootstrap, respectively. The bird community varied in species richness, abundance and diversity from year to year. As for species diversity, 1991, 1993 and 1994 were significantly different from the other years. Changes in the landscape contributed to the increase in abundance and richness for the groups of forest, open-area and bamboo-specialist species. An important factor discussed was the effect of the flowering of "taquara" (Poaceae), which contributed significantly to increasing richness of bamboo seed eaters, mainly in 1992 and 1993. In general, the results showed that landscape changes affected the dynamics and structure of the bird community of this forest fragment over time, and proved to have an important role in conservation of the avian community in areas of intensive forestry and agricultural activities.

Bird communities in three forest types in the Pernambuco Centre of Endemism, Alagoas, Brazil

The Pernambuco Center of Endemism (PCE) in northeastern Brazil is highly fragmented and degraded. Despite its potential conservation importance the bird fauna in this area is still relatively unknown and there are many remnant fragments that have not been systematically surveyed. Here, we report the results of bird surveys in five forest fragments (one pioneer, two ombrophilous and two seasonal). In total, 162 taxa were recorded, 12 of which are endemic to the PCE. The frequency of endangered species was lower than what has been reported in studies from the same area and most of the taxa considered to be at risk of extinction were sub-species of uncertain taxonomic validity. The comparatively low number of endemic/threatened species may be due to the small size of the fragments in the present study - a consequence of the high levels of habitat loss in this region. Analysis of species richness patterns indicates that ombrophilous forest fragments are acting as refuges for those bird species that are most sensitive to environmental degradation.

Effects of anthropogenic impacts on benthic macroinvertebrates assemblages in subtropical mountain streams

The nature of the riparian and surrounding landscape has been modified by anthropogenic activities, which may subsequently alter the composition and functional structure of macroinvertebrate assemblages. The effect of these changes on function of benthic fauna is difficult to assess due to the scarce knowledge on functional structures in tropical streams. In this study we evaluate whether sites impacted and unimpacted by anthropogenic alterations differed in assemblage composition and density, richness and diversity of each functional feeding group. The selection of the sites was related to their distinct riparian characteristics, following the QBRy riparian quality index. Collector-gatherer was the dominant functional feeding group, comprising 91% of total density, whereas the proportion of shredders was very low, representing less of 0.5% of total density. Asemblage composition of macroinvertebrates differed between impacted and unimpacted sites. Predators were dominant in taxa number, representing about 60% of total taxa richness. In addition, the diversity and richness of collector-gatherers differed significantly between degraded and unimpacted sites, reflecting the sensitivity of this group to environmental changes and the utility to be used in the assessment of anthropogenic modifications. The results of this study reinforce the idea that riparian corridor management is critical for the distribution of macroinvertebrate assemblages as well as functional organization of lotic streams.

Diet of Procyon cancrivorus (Carnivora, Procyonidae) in restinga and estuarine environments of southern Brazil

Despite its wide range and abundance on certain habitats, the crab-eating raccoon Procyon cancrivorus (G. Cuvier, 1798) is considered one of the less known Neotropical carnivore species. In the present study we analyzed the diet of P. cancrivorus in a peat forest and in an estuarine island in southernmost Brazil. Fruits of the gerivá palm tree Syagrus romanzoffiana were the most consumed item in the peat forest, followed by insects and mollusks. Small mammals, followed by Bromelia antiacantha (Bromeliaceae) fruits and brachyuran crustaceans were the most frequent items in the estuarine island. Other items found in lower frequencies were Solanum sp., Psidium sp., Smilax sp. and Dyospiros sp. fruits, diplopods, scorpions, fishes, anuran amphibians, reptiles (black tegu lizard and snakes), birds and medium-sized mammals (white-eared opossum, armadillo and coypu). Levin’s index values (peat forest: 0.38; estuarine island: 0.45) indicate an approximation to a median position between a specialist and a well distributed diet. Pianka’s index (0.80) showed a considerable diet similarity between the two systems. Procyon cancrivorus presented a varied diet in the studied areas and may play an important role as seed disperser on coastal environments in southernmost Brazil.

Diversity and habitat preference of medium and large-sized mammals in an urban forest fragment of southwestern Amazon

We assessed the species composition and abundance of medium and large-sized mammals in an urban forest fragment in the Brazilian Amazon, and recorded the preference of some species for particular phytophysiognomies. We placed nine transects with 20 sand plots each in three phytophysiognomies: open rainforest with a dominance of bamboos (OFB), open rainforest with palm trees (OFP), and dense rainforest (DF). We calculated species abundance as the number of records/plot.day, in a total of 2,700 plots.day. We recorded twelve mammal species; Sylvilagus brasiliensis (Linnaeus, 1758) and Dasyprocta fuliginosa (Wagler, 1831) were the most abundant. The results differed among phytophysiognomies: DF presented the highest mammal diversity, whereas the species composition of OFP was less similar than that of other phytophysiognomies. Rodents showed higher preference for OFP and Sylvilagus brasiliensis was more abundant in OFB. The study area showed high species richness, with the occurrence of mesopredators, but there was a predominance of common species adaptable to disturbed environments, which reflects the severe isolation degree of the forest fragment and the hunting pressure that is still present.

Diversity and population characteristics of terrestrial isopods (Crustacea, Oniscidea) across three forest environments in southern Brazil

Terrestrial isopods are important and dominant component of meso and macrodecomposer soil communities. The present study investigates the diversity and species composition of terrestrial isopods on three forests on the Serra Geral of the state of Rio Grande do Sul, Brazil. The area has two natural formations (Primary Woodland and Secondary Woodland) and one plantation of introduced Pinus. The pitfall traps operated from March 2001 to May 2002, with two summer periods and one winter. There were 14 sampling dates overall. Of the five species found: Alboscia silveirensis Araujo, 1999, Atlantoscia floridana (van Name, 1940), Benthana araucariana Araujo & Lopes, 2003 (Philoscidae), Balloniscus glaber Araujo & Zardo, 1995 (Balloniscidae) and Styloniscus otakensis (Chilton, 1901) (Styloniscidae); only A. floridana is abundant on all environments and B. glaber is nearly exclusive for the native forests. The obtained data made it possible to infer about population characteristics of this species. The Similarity Analysis showed a quantitative difference among the Secondary forest and Pinus plantation, but not a qualitative one. The operational sex ratio (OSR) analysis for A. floridana does not reveal significant differences in male and female proportions among environments. The reproductive period identified in the present study for A. floridana was from spring to autumn in the primary forest and Pinus plantation and during all year for the secondary forest. The OSR analysis for B. glaber reveals no significant differences in abundance between males and females for secondary forest, but the primary forest was a significant difference. The reproductive period for B. glaber extended from summer to autumn (for primary and secondary forest). This is the first record for Brazil of an established terrestrial isopod population in a Pinus sp. plantation area, evidenced by the presence of young, adults and ovigerous females, balanced sex ratio, expected fecundity and reproduction pattern, as compared to populations from native vegetation areas.

Ecological relations between mangrove leaf litter and the spatial distribution of the gastropod Melampus coffeus in a fringe mangrove forest

Leaf litter represents a food source to many organisms that may directly contribute to organic matter decomposition. In addition, the physical presence of these vegetal detritus contributes for the modification of some environmental areas and produce microhabitats that may act as a refuge against predators and desiccation for many animals. The pulmonate gastropod Melampus coffeus (Linnaeus, 1758) (Ellobiidae) is a very common specie in Atlantic Coast mangrove forests and feeds on fallen mangrove leaves. It was hypothesized that the spatial distribution of Melampus coffeus is directly affected by mangrove leaf litter biomass deposition. Thus, this research aimed at evaluating the spatial distribution of these gastropods in relation to the biomass of mangrove leaf litter through a twelve-month period. The study area was established in the middle estuary of Pacoti River, state of Ceará, Brazil where two adjacent zones with different topographic profiles were determined. Samples of Melampus coffeus and leaf litter were collected monthly, throughout a year, from the mangrove ground surface. The results indicated that the presence of twigs in mangrove litter favor the occupation by smaller individuals of M. coffeus, probably because smaller individuals are more susceptible to predator attacks and desiccation than larger ones, and twigs and branches may provide a safe microhabitat.

Use of tree species by White-throated treerunner (Pygarrhichas albogularis King) in a secondary native forest of southern Chile

ABSTRACT In forest ecosystems, numerous species of insectivorous birds use certain tree species as feeding and nesting substrates. Between 2009 and 2010, the use of different floristic components as feeding substrate by the Pygarrhichas albogularis King, 1831 was evaluated in a southern Chilean secondary native forest. From a total of 13 trees and bush species, six tree species were used by P. albogularis as a feeding substrate. Tree use was limited to intermediate heights (11-20 m) and, mainly, to the trunk (40% of observations) and secondary branches (26%). Pygarrhichas albogularis showed a disproportionated use of N. dombeyi and an important use of trees with a greater age structure (DBH 81-100 cm). Nothofagus dombeyi presented a significantly greater tree bark crevice depth than E. cordifolia. In turn, covariance between crevice depth and invertebrate supply in tree bark was positive and significant. We consider bark depth and invertebrate supply to be the proximate causes explaining P. albogularis disproportionated use of Nothofagus dombeyi.

Density estimates and conservation of Leopardus pardalis southernmost population of the Atlantic Forest

ABSTRACT Using camera traps and capture/recapture analyses we recorded the presence and abundance of cat species at Turvo State Park, in southern Brazil. Ocelot [Leopardus pardalis (Linnaeus, 1758)] population density was estimated for two areas of the park, with differing management profiles. Density estimates varied from 0.14 to 0.26 indiv. km2. Another five cat species were recorded at very low frequencies, precluding more accurate analyses. We estimate 24 to 45 ocelots occur in the reserve, which is probably too small for long-term maintenance of the population, if isolated. However, if habitat integrity and connectivity between the Park and the Green Corridor of Misiones is maintained, an estimated ocelot population of 1,680 individuals should have long-term viability.

The vertical dispersión of Anopheles (Kerteszia) cruzi in a forest in southern Brazil suggests that human cases of malaria of simian origin might be expected

By staining females of Anopheles cruzi with fluorescent coloured powders in a forest in the State of Santa Catarina, we showed that they move from canopy to ground and vice-versa to feed. This suggests that in areas where this mosquito is a vector of human and simian malarias sporadic infections of man with monkey plasmodia might be expected.

Ovipositional substrates used by Calyptrate Diptera in Tijuca forest, Rio de Janeiro

Twenty seven species of calyptrate muscoids were reared from a forested area of Rio de Janeiro (Tijuca Forest). Substrates for obtaining flies were beef liver, fish, mouse, frog, shrimp, snail carcasses, human faeces, banana and papaya fruits. The most frequent species found were: Fannia sp. (subgroup pusio) (49.9% on shrimp). Hemilucilia flavifacies (95.0% on liver). Phaenicia eximia (49.4% on mouse), Synthesiomyia nudiseta ( 100.0% on fish), Ophyra aenescens (100.0% on shrimp), Oxyvinia excisa (100.0% on faeces), Euboettecheria collusor (52.4% on faeces) and Pattonella intermutans (61.0% on frog).