In vitro antimicrobial activity of a new series of 1,4-naphthoquinones

The antibacterial activity of a series of 1,4-naphthoquinones was demonstrated. Disk diffusion tests were carried out against several Gram-positive and Gram-negative bacteria. The compound 5-amino-8-hydroxy-1,4-naphthoquinone was the most effective, presenting inhibition zones measuring 20 mm against staphylococci, streptococci and bacilli at 50 µg/ml. Methicillin-resistant Staphylococcus aureus and several clinical isolates of this bacterium were also inhibited. Naphthazarin, 5-acetamido-8-hydroxy-1,4-naphthoquinone, and 2,3-diamino-1,4-naphthoquinone were the next most active compounds. The minimal inhibitory concentration of the active compounds was determined against S. aureus, ranging from 30 to 125 µg/ml. All compounds presented a minimal bactericidal concentration higher than 500 µg/ml, indicating that their effect was bacteriostatic. The EC50, defined as the drug concentration that produces 50% of maximal effect, was 8 µg/ml for 5-amino-8-hydroxy-1,4-naphthoquinone against S. aureus, S. intermedius, and S. epidermidis. These results indicate an effective in vitro activity of 5-amino-8-hydroxy-1,4-naphthoquinone and encourage further studies for its application in antibiotic therapy.

Sentinel node biopsy in breast cancer: results in a large series

Sentinel lymph node biopsy (SLNB) is an appropriate method for the evaluation of axillary status in cases of early breast cancer. We report our experience in treating cases evaluated using SLNB. We analyzed a total of 1192 cases assessed by means of SLNB from July 1999 to December 2007. SLNB processing was successfully completed in 1154 cases with the use of blue dye or radiolabeled 99mTc-Dextran-500, or both. Of these 1154 patients, 857 were N0(i-) (no regional lymph node metastasis, negative immunohistochemistry, IHC), 96 were N0(i+) (no regional lymph node metastasis histologically, positive IHC, no IHC cluster greater than 0.2 mm) and 201 were N1mi (greater than 0.2 mm, none greater than 2.0 mm). Most of the tumors (70%) were invasive ductal carcinomas and tumors were staged as T1 in 770 patients (65%). A total of 274 patients underwent SLNB and axillary dissections up to April 2003. The inclusion criteria were tumor size equal to or less than 3 cm in diameter, no clinically palpable axillary lymph nodes, no neoadjuvant therapy. In 19 cases, the SLN could not be identified intraoperatively. A false-negative rate of 11% and a negative predictive value of 88.2% were obtained for the 255 assessable patients. The overall concordance between SLNB and axillary lymph node status was 92%. SLNB sensitivity for nodes was 81% and specificity was 100%. The higher sensitivity, specificity, accuracy, and lower false-negative rates of SLNB suggest that this method may be an appropriate alternative to total axillary dissection in early breast cancer patients.

Early detection of metabolic and energy disorders by thermal time series stochastic complexity analysis

Maintenance of thermal homeostasis in rats fed a high-fat diet (HFD) is associated with changes in their thermal balance. The thermodynamic relationship between heat dissipation and energy storage is altered by the ingestion of high-energy diet content. Observation of thermal registers of core temperature behavior, in humans and rodents, permits identification of some characteristics of time series, such as autoreference and stationarity that fit adequately to a stochastic analysis. To identify this change, we used, for the first time, a stochastic autoregressive model, the concepts of which match those associated with physiological systems involved and applied in male HFD rats compared with their appropriate standard food intake age-matched male controls (n=7 per group). By analyzing a recorded temperature time series, we were able to identify when thermal homeostasis would be affected by a new diet. The autoregressive time series model (AR model) was used to predict the occurrence of thermal homeostasis, and this model proved to be very effective in distinguishing such a physiological disorder. Thus, we infer from the results of our study that maximum entropy distribution as a means for stochastic characterization of temperature time series registers may be established as an important and early tool to aid in the diagnosis and prevention of metabolic diseases due to their ability to detect small variations in thermal profile.

A time series analysis of meteorological factors and hospital outpatient admissions for cardiovascular disease in the Northern district of Guizhou Province, China

Findings on the effects of weather on health, especially the effects of ambient temperature on overall morbidity, remain inconsistent. We conducted a time series study to examine the acute effects of meteorological factors (mainly air temperature) on daily hospital outpatient admissions for cardiovascular disease (CVD) in Zunyi City, China, from January 1, 2007 to November 30, 2009. We used the generalized additive model with penalized splines to analyze hospital outpatient admissions, climatic parameters, and covariate data. Results show that, in Zunyi, air temperature was associated with hospital outpatient admission for CVD. When air temperature was less than 10°C, hospital outpatient admissions for CVD increased 1.07-fold with each increase of 1°C, and when air temperature was more than 10°C, an increase in air temperature by 1°C was associated with a 0.99-fold decrease in hospital outpatient admissions for CVD over the previous year. Our analyses provided statistically significant evidence that in China meteorological factors have adverse effects on the health of the general population. Further research with consistent methodology is needed to clarify the magnitude of these effects and to show which populations and individuals are vulnerable.

Julgar sem preceptivas, julgar pelo universal: glosas modernistas a Kant

Este artigo busca pensar os juízos estéticos da Terceira crítica de Kant, em relação aos discursos sobre a arte moderna. Propomos, de maneira didática, relacionar tais juízos kantianos à utopia da modernidade e às suas consequências (e críticas) contemporâneas. Não tencionamos, porém, fazer uma análise pormenorizada do pensamento de Kant, propriamente, mas expor as várias glosas que este recebeu, ao inaugurar um outro espaço para a Estética, principalmente no que se refere à arena comunitária na qual se agenciam os juízos e que torna possível uma arte ao mesmo tempo universal e sem regras fixas. Mais do que pensar a arte como "livre jogo", ou como "bela" e como "finalidade sem fim", pontos nodais da crítica de Kant, sem dúvida, interessa-nos investigar a perspectiva autorreflexiva que os juízos estéticos permitem. A interpretação do objeto-arte moderno não seria, em última instância, o exercício comunitário de um juízo?

Por uma antropologia de alcance universal

Partindo do pressuposto de que a antropologia e a educação tomam o homem como base comun de reflexão, o texto propõe uma discussão sobre as possíveis diferenças entre as abordagens desses campos do conhecimento, considerando a escolha do lugar teórico a partir do qual uma propósta investigativa é conduzida como o aspecto decisivo da questão. A discussão é estruturada a partir de uma crítica ao discurso pós-moderno que adere ao relativismo - historicamente associado ao sucesso público da antropologia no estudo da diversidade -, e se espraia por todas as ciências humanas no embalo do que se convencionou chamar de "crise de paradigmas", Argumenta-se que sem abrir da característica que dirige o trabalho dos antropólogos, qual seja, o profundo conhecimento de objetos singulares, não se pode furtar ao compromisso científico de inseri-los num contexto mais amplo de compreensão, numa perspectiva passível de encontrar ressonância entre outros pesquisadores sociais. A articulação dos valores universais e das especificidades culturais enquanto dimensões de uma mesma realidade, além de ser uma exigência teórica, impõe-se praticamente na medida em que a democracia passa a ser desafiada pelo movimento global da pobreza, exclusão social e surgimento de particularismos absolutos.