Influence of angiotensin II receptor subtypes of the paraventricular nucleus on the physiological responses induced by angiotensin II injection into the medial septal area

OBJECTIVE: We determined the effects of losartan and PD 123319 (antagonists of the AT1 and AT2 angiotensin receptors, respectively), and [Sar¹, Ala8] ANG II (a relatively peptide antagonist of angiotensin receptors) injected into the paraventricular nucleus (PVN) on water and 3% NaCl intake, and the diuretic, natriuretic, and pressor effects induced by administration of angiotensin II (ANG II) into the medial septal area (MSA) of conscious rats. METHODS: Holtzman rats were used . Animals were anesthetized with tribromoethanol (20 mg) per 100 grams of body weight, ip. A stainless steel guide cannula was implanted into the MSA and PVN. All drugs were injected in 0.5-mul volumes for 10-15 seconds. Seven days after brain surgery, water and 3% NaCl intake, urine and sodium excretion, and arterial blood pressure were measured. RESULTS: Losartan (40 nmol) and [Sar¹, Ala8] ANG II (40 nmol) completely eliminated whereas PD 123319 (40 nmol) partially blocked the increase in water and sodium intake and the increase in arterial blood pressure induced by ANG II (10 nmol) injected into the MSA. The PVN administration of PD 123319 and [Sar¹, Ala8] ANG II blocked whereas losartan attenuated the diuresis and natriuresis induced by MSA administration of ANG II. CONCLUSION: MSA involvement with PVN on water and sodium homeostasis and arterial pressure modulation utilizing ANGII receptors is suggested.

Effects of high glucose concentrations on the endothelial function of the renal microcirculation of rabbits

OBJECTIVE: To assess the acute effects of high glucose concentrations on vascular reactivity in the isolated non diabetic rabbit kidney. METHODS: Rabbits were anaesthetized for isolation of the kidneys. Renal arteries and veins were cannulated for perfusion with Krebs-Henselleit solution and measurement of perfusion pressure. After 3 hours of perfusion with glucose 5,5 mM (control ) and 15 mM, the circulation was submitted to sub maximal precontraction (80% of maximal response) trough continuous infusion of noradrenaline 10 mM. Vascular reactivity was then assessed trough dose-responses curves with endothelium-dependent (acetylcholine) and independent (sodium nitroprusside) vasodilators. The influence of hyperosmolarity was analyzed with perfusion with mannitol 15mM. RESULTS: A significant reduction in the endothelium-dependent vasodilation in glucose 15mM group was observed compared to that in control, but there was no difference in endothelium-independent vasodilation. After perfusion with mannitol 15 mM, a less expressive reduction in endothelium-dependent vasodilation was observed, only reaching significance in regard to the greatest dose of acetylcholine. CONCLUSION: High levels of glucose similar to those found in diabetic patients in the postprandial period can cause significant acute changes in renal vascular reactivity rabbits. In diabetic patients these effects may also occur and contribute to diabetes vascular disease.

Treatment of Dyslipidemia with Statins and Physical Exercises: Recent Findings of Skeletal Muscle Responses

Statin treatment in association with physical exercise practice can substantially reduce cardiovascular mortality risk of dyslipidemic individuals, but this practice is associated with myopathic event exacerbation. This study aimed to present the most recent results of specific literature about the effects of statins and its association with physical exercise on skeletal musculature. Thus, a literature review was performed using PubMed and SciELO databases, through the combination of the keywords “statin” AND “exercise” AND “muscle”, restricting the selection to original studies published between January 1990 and November 2013. Sixteen studies evaluating the effects of statins in association with acute or chronic exercises on skeletal muscle were analyzed. Study results indicate that athletes using statins can experience deleterious effects on skeletal muscle, as the exacerbation of skeletal muscle injuries are more frequent with intense training or acute eccentric and strenuous exercises. Moderate physical training, in turn, when associated to statins does not increase creatine kinase levels or pain reports, but improves muscle and metabolic functions as a consequence of training. Therefore, it is suggested that dyslipidemic patients undergoing statin treatment should be exposed to moderate aerobic training in combination to resistance exercises three times a week, and the provision of physical training prior to drug administration is desirable, whenever possible.

Nota sôbre um possível fator sub-letal em gado Holstein-Friesian

In this note the A. A. relate the occurrence of a possible sub-lethal factor, on the Holstein-Friesian herd of Escola Superior de Agricultura "Luiz de Queiroz", Piracicaba. The sire Horto was mated with his own mother, Brisa, and so, were obtained two calves, a male and a female, consecutively. Both the calves presented flexion and deviation of the fore legs. The sire's death has not alloved further observations. The study of these history cases excludes the mother's nutritional deficiency, as the cause of related phenomenon. In the consulted literature, VEIGA and MEAD et al. relate similar cases, although these are observed in other breeds of cattle The A. A. admit that cause of occurrence is a possible sublethal recessive factor, put in evidence by inbreeding.

Desempenho de bovinos confinados com ração ã base de bagaço de cana-de-açúcar auto-hidrolisado, levedura e vinhaça, sub-produtos da indústria de açúcar e álcool

O uso de levedura seca e concentrada de vinhaça, associados ao bagaço de cana-de-açúcar auto-hi drolisado (BAH) em rações de confinamento, foi testado em comparação com rações também à base de BAH, contendo farelo de algodão como fonte de proteína. Durante 122 dias foi conduzido ura experimento de ganho de peso com novilhos confinados (peso vivo inicial = 316kg). As rações, formuladas para proporcionarem ganho de peso lkg por dia, continham 50% de BAH, 12,7% da fonte de proteína (farelo de algodão ou concentrado de vinhaça ou levedura), 17,8% de milho e 12,5% de cana picada como volumoso complementar, na materia seca (M.S.). 0 ganho de peso médio foi de 843, 989 e 580g/cabeça/dia para as rações contendo farelo de algodão, levedura e concentrado de vinhaça, respectivamente. As rações contendo levedura e farelo de algodão proporcionaram ganhos de peso significativamente superiores (R <_ 0,01) quando comparadas à ração contendo concentrado de vinhaça, não ocorrendo diferenças significativas entre as duas primeiras, nem mesmo ao nível de 5% pelo teste de Tukey. Conclui-se que a levedura seca pode substituir totalmente o farelo de algodão em rações completas para confinamento contendo bagaço de cana auto-hidrolisado, devendo-se, entretanto, considerar os aspectos econômicos dessa substituição.

Dial behavioral responses of Metamysidopsis elongata atlantica (Crustacea, Mysida) to gradients of salinity and temperature

Metamysidopsis atlantica elongata (Bascescu, 1968) is a common mysid in the surf zone of sandy beaches from the state of Rio Grande do Sul, Brazil, where it is frequently recorded forming dense aggregations. Trough laboratory trials, behavioral responses to salinity (10, 20, 25, 28, 30, 40 e 45), temperature (10, 15, 20, 30±1ºC) and light (yes/no) were tested using adult males, adult females and juveniles. Although there was no response to temperature, the species showed clear response to salinity and light. In the presence of light, organisms remained in the bottom of the aquaria, but moved to surface when bottom salinities were increased. In the absence of light, adults moved to the surface. However, juveniles moved down to or remained on the bottom, maybe as a response to avoid adult predation.

Os anofelinos do sub-gênero kerteszia em relação à distribuição das bromeliáceas em comunidades florestais do município de Brusque, Estado de Santa Catarina

The present work is part of the studies realized under the authority of the National Service of Malaria (Brazil), with the collaboration of scientists of the Oswaldo Cruz Institute, in some forests of the southern part of Brazil.This is the first of a series and its subject is the development of the Anopheles mosquitoes of the kerteszia in water collected in Bromeliaceae leaves. The ecology of Bromeliaceae was studied in a previous work. The botanical material was classified by specialists from several botanical institutions from Europe and the United States of America. The most important ecological relations of the “bromeliad-kerteszia” problem were presented through four indices: 1st Positivity index – Relative frequency of bromeliad with watery forms in the bromeliad examined. 2nd Larval index – Mean number of watery forms in the positive bromeliad. 3rd Ovoposition index – Product of the Positivy index by the Larval index. 4th MK index – Product of the Ovoposition index by the total number of bromeliad, positive or not, in a unity of area (1.000 m²). The capture of flying forms in relation to the relative humidity was also studied. From the several forests of the Brusque region we have selected one community of each type, which were the most representative forests in Southern Brazil. Conclusions on the “bromeliad-kerteszia” problem – From a general point of view only a few factors are really important and these are listed below: 1°) The volum of water on the bromeliad. 2°) The level where the bromeliad is fixed. 3°) The number of bromeliad in unity of area. The distribution of microclimas in the forest through the considered levels has a direct influence on the species of subgenus Kerteszia (qualitative influence) and an indirect influence through the ecological distribution of the more frequent bromeliad with best qualities as biotope for the watery forms (qualitative influence). The MK index is roughly proportional to the square of half the total number of Bromeliaceae in a certain type of forest. Then the MK index would be a certain function of the ecological type of the forest and of the total number of bromeliad in a unity of area. MK approximately α x (x/10)² . x = n° of bromeliad in a unity of área (1.000 m²); α = qualitative factor. It would be interesting to see if this proportion is maintained when we have examined a greater number of forests of different types.