Infestation and natural parasitism of aphids in single and mixed pastures of black oats and ryegrass

Some species of aphids are major pests on cereal crops and grass pastures. Usually these pests are not adequately controlled in pasture lands that become sources of aphid infestations to cereal crops. The dynamics of aphids and the incidence of natural enemies are less known in pasture systems than in cereal fields. The objective of this work was to assess the aphid infestation and natural aphid parasitism in different pasture composition. Three hypotheses were tested: 1- the aphid species composition in pastures may vary according to the cereal species in the field; 2- the mixture of two plant species can modify the amount and diversity of aphids; 3- the plant species composition of pasture fields influences the parasitism of aphids. Empirical data were obtained from three Poaceae fields: black oats (Avena strigosa L.), ryegrass (Lolium multiflorum L.), and a mixed field of black oats and ryegrass. The most abundant aphid species was Rhopalosiphum padi followed by Sitobion avenae. Plant species composition increases the amount and the parasitism rates of aphids. The mixture of heavily infested black oats with a poorly infested ryegrass resulted in reduction of aphid infestation in comparison with heavily-infested single plant species field. This is possible because the conditions are favorable for the development of parasitoid populations. Aphidius colemani was the main parasitoid found in these areas.

Atributos microbianos do solo após a adição de lodo anaeróbio da estação de tratamento de efluentes de parboilização do arroz

O cultivo do arroz é destaque na região Sul, com 60 % da produção total desse cereal no país. A parboilização do arroz é um tratamento hidrotérmico que cozinha parcialmente os grãos ainda em casca, melhorando seu valor nutricional. Desse processo resulta um efluente rico em matéria orgânica e nutrientes, que pode ser utilizado como adubo orgânico na agricultura. O presente trabalho objetivou avaliar a qualidade de um solo com base nas alterações de alguns atributos microbianos decorrentes do acréscimo de lodo anaeróbio da estação de tratamento de efluentes de parboilização do arroz. Foram realizados dois experimentos, sendo o primeiro em casa de vegetação, por meio do cultivo de milho em um Argissolo acrescido de lodo (base seca) nas doses de 2,15; 4,31; 8,62; 12,93; e 17,24 g kg-1, em que foram analisados os teores de C orgânico total (COT) e N total (NT)do solo e da biomassa microbiana. O segundo foi desenvolvido em laboratório, utilizando-se vasos respirométricos, com o mesmo solo e tratamentos do experimento anterior, onde se analisou a atividade da microbiota do solo pela determinação da respiração basal. Como tratamentos controle foram utilizados: solo sem adubação e solo adubado com NPK. Os tratamentos foram dispostos em delineamento completamente casualizado com quatro repetições. A atividade microbiana do solo e o C (CM) e N (NM) da biomassa microbiana aumentaram com as doses de lodo e proporcionaram diminuição no quociente metabólico do solo. A aplicação do lodo não alterou as relações COT/NT, CM/NM, CM/COT e NM/NT, em comparação com o tratamento NPK. A aplicação do lodo ao solo aumentou os teores de COT e causou diminuição nos teores de N mineral (NH4+ e NO3-), quando comparados aos do tratamento NPK. O lodo originado da parboilização do arroz pode ser aplicado ao solo, em doses recomendadas, com benefícios à atividade e à biomassa microbiana.

Ammonia volatilization from nitrogen fertilizers in no-till wheat and maize in southern Brazil

Crop residues on the soil surface of no-till systems can intensify ammonia volatilization from N fertilizers applied to cereal crops. This study assessed the magnitude of N losses through ammonia volatilization from urea applied to no-till winter (wheat) and summer crops (maize) on a Typic Hapludox in the south-central region of Paraná, southern Brazil. In addition, the potential of alternative N sources (urea with urease inhibitor, liquid fertilizer, ammonium nitrate and ammonium sulfate) and different urea managements (fertilizer applied in the morning or afternoon) were evaluated. Two experiments with maize and wheat were carried out for two years, arranged in a randomized block design with four replications. Nitrogen volatilization losses were assessed with a semi-open static collector until 21 days after fertilization. In winter, the losses were low (<5.5 % of applied N) for all N sources, which were not distinguishable, due to the low temperatures. In the summer, volatilization rates from urea were higher than in the winter, but did not exceed 15 % of applied N. The main factor decreasing N losses in the summer was the occurrence of rainfall in the first five days after fertilization. Urea with urease inhibitor, nitrate and ammonium sulfate were efficient to decrease ammonia volatilization in maize, whereas the application time (morning or afternoon) had no influence.

Molecular Identification of Trichoderma spp. in Garlic and Onion Fields and In Vitro Antagonism Trials on Sclerotium cepivorum

ABSTRACT Trichoderma species are non-pathogenic microorganisms that protect against fungal diseases and contribute to increased crop yields. However, not all Trichoderma species have the same effects on crop or a pathogen, whereby the characterization and identification of strains at the species level is the first step in the use of a microorganism. The aim of this study was the identification – at species level – of five strains of Trichoderma isolated from soil samples obtained from garlic and onion fields located in Costa Rica, through the analysis of the ITS1, 5.8S, and ITS2 ribosomal RNA regions; as well as the determination of their individual antagonistic ability over S. cepivorum Berkeley. In order to distinguish the strains, the amplified products were analyzed using MEGA v6.0 software, calculating the genetic distances through the Tamura-Nei model and building the phylogenetic tree using the Maximum Likelihood method. We established that the evaluated strains belonged to the species T. harzianum and T. asperellum; however it was not possible to identify one of the analyzed strains based on the species criterion. To evaluate their antagonistic ability, the dual culture technique, Bell’s scale, and the percentage inhibition of radial growth (PIRG) were used, evidencing that one of the T. asperellum isolates presented the best yields under standard, solid fermentation conditions.

Influência do manejo do solo e da rotação de culturas na qualidade industrial do trigo

A qualidade industrial do trigo é afetada por fatores genéticos e ambientais, como solo, clima, tratos culturais, e outros. O presente trabalho teve como finalidade avaliar o efeito de sistemas de manejo de solo e de rotação de culturas sobre características que definem a qualidade industrial de trigo (peso do hectolitro, peso de mil grãos, extração experimental de farinha, alveografia, teste de microssedimentação com lauril sulfato de sódio e número de queda). O delineamento experimental foi em blocos ao acaso, com parcelas subdivididas e três repetições. A parcela principal foi constituída pelos sistemas de manejo de solo (plantio direto, preparos convencionais de solo com arado de discos e de aivecas, e cultivo mínimo), e a subparcela, pelos sistemas de rotação de culturas (monocultura, um inverno e dois invernos sem trigo). O preparo convencional de solo com arado de discos e o cultivo mínimo reduziu o número de queda; o sistema de rotação com dois invernos sem trigo elevou o peso do hectolitro; a monocultura desse cereal reduziu o peso do hectolitro, elevou a força geral de glúten e a microssedimentação com lauril sulfato de sódio; a interação sistemas de manejo de solo, rotação de culturas e ano de cultivo afetou o peso de mil grãos e, o ano de cultivo teve grande influência em todas as características de qualidade industrial de trigo estudadas.

Effect of intercropping wheat with forage legumes on wheat production and ground cover

The use of winter legumes in southern Brazil is hindered by the slow growth of these species during establishment exposing soil surface to erosion. Introduction of these species along with spring wheat (Triticum aestivum L.) was studied as a means of increasing ground cover during their initial establishment period, without reducing wheat grain yield. Two experiments were conducted in nearby areas, one in each year. Birdsfoot trefoil (Lotus corniculatus L.), red clover (Trifolium pratense L.) cultivar Quiñequelli, white clover (T. repens L.), and arrowleaf clover (T. vesiculosum Savi) did not reduce cereal yield in either year. Wheat yield was reduced by intercropped red clover cultivar Kenland and by subclover (T. subterraneum L.) in the first year. No grain yield differences due to intercropping with any legume were detected in the second year, when rainfall was below normal. Intercropping with wheat showed to be a practical alternative to enhance ground cover at establishing forage legumes.

Influence of micronutrients on dry matter yield and interaction with other nutrients in annual crops

The objective of this work was to determine the influence of Zn, Mn and Cu on shoot dry matter yield and uptake of macro and micronutrients in upland rice, common bean and corn. Six greenhouse experiments were conducted using a Dark Red Latosol (Typic Haplusthox). Treatments consisted of application of Zn at 0, 5, 10, 20, 40, 80 and 120 mg kg-1, of Mn at 0, 10, 20, 40, 80, 160, 320 and 640 mg kg-1 and of Cu application at 0, 2, 4, 8, 32, 64 and 96 mg kg-1. Zinc increased yield of rice, Mn increased yields of corn and bean and Cu improved yields of rice and bean. Uptake of N, Ca, and Cu in rice was decreased by zinc treatment. In common bean, uptake of N, Mg, and Cu was increased by zinc application, whereas, uptake of P was decreased. Manganese increased uptake of Mg, Zn and Fe and decreased uptake of Ca, in corn. Uptake of K, Zn and Mn was increased and uptake of P and Cu was decreased by Mn application, in bean. Copper had positive and negative interactions in the uptake of macro and micronutrients, depending on crop species and nutrients involved.

Effects of plant population and nitrogen fertilizer on yield and efficiency of maize-bean intercropping

Nitrogen supply and plant population are basic parameters for cereal-legume intercropping. In order to study plant population and nitrogen fertilizer effects on yield and yield efficiency of maize-bean intercropping, a field experiment was established. Three bean plant populations and three nitrogen levels were used. Maize dry matter accumulation decreased with increases in bean plant population. Competitive effect of intercrop beans on maize yields was high at higher plant populations, being decreased by nitrogen fertilizer; application of 50 kg ha-1 N was very efficient in increasing maize cob yield. Intercropping significantly decreased harvest index of beans in all plant population and nitrogen fertilizer situations. The efficiency of intercropping, compared to sole cropping, was evidenced by the values obtained for Land Equivalent Ratio (LER) for biomass, cob and pod yields that increased with increases in bean plant populations and nitrogen fertilizer levels.

Nitrogen fixation by groundnut and velvet bean and residual benefit to a subsequent maize crop

Chemical fertilisers are rarely avaiable to poor farmers, for whom the nitrogen (N) is often the most limiting element for cereal grain production. The objective of this study was to quantify the contribution of biological nitrogen fixation (BNF) to groundnut (Arachis hypogaea) and velvet bean (Mucuna pruriens) crops using the 15N natural abundance (delta15N) technique and to determine their residual effect and that of a natural fallow, on growth and N accumulation by two rustic maize varieties. The contribution of BNF calculated from delta15N data was 40.9, 59.6 and 30.9 kg ha-1, for groundnut, velvet bean and the natural fallow, respectively. The only legume grain harvested was from the groundnut, which yielded approximately 1.000 kg ha-1. The subsequent maize varieties ("Sol de Manhã" and "Caiana Sobralha") yielded between 1.958 and 2.971 kg ha-1, and were higher after velvet bean for both maize varieties and "Sol da Manhã" groundnut, followed by "Caiana" after groundnut and, finally, the natural fallow. For a small-holder producer the most attractive system is the groundnut followed by maize, as, in this treatment, both groundnut and maize grain harvest are possible. However, a simple N balance calculation indicated that the groundnut-maize sequence would, in the long term, deplete soil N reserves, while the velvet bean-maize sequence would lead to a build up of soil nitrogen.

MN 716: nova cultivar de cevada com estabilidade de produção e qualidade cervejeira

A cevada MN 716, lançada em 2004, é uma das cultivares recomendadas para cultivo com maior estabilidade de produção. Nos anos em que participou do ensaio para determinação de seu valor de cultivo e uso, apresentou excelente produtividade de grãos, com média superior a 3.000 kg ha-1, e desempenho equilibrado quanto à qualidade, com destaque para seu teor de beta-glucanas e índice enzimático. A cultivar constitui um avanço do melhoramento desse cereal no Brasil, aliando características de interesse do produtor e da indústria.

Occurrence of Aceria tosichella in Brazil

The objective of this work was to evaluate the occurrence of Aceria tosichella Keifer (Prostigmata: Eriophyidae), the wheat curl mite, in Rio Grande do Sul, Brazil. Samples to detect A. tosichella specimens were collected in wheat, corn, oat crops and potential host grasses in 46 localities, in October 2006, August and October 2007. Samples of wheat were also collected in experimental greenhouses at Embrapa Trigo, Passo Fundo, RS, Brazil. A. tosichella specimens were found in wheat samples from Passo Fundo, Palmeira das Missões, São Luís Gonzaga, and Santo Antônio das Missões, RS, Brazil. Symptoms due to A. tosichella infestations were observed only in greenhouse conditions. This is the first report of A. tosichella in Brazil and the second in South America.

Spittlebug impacts on sugarcane quality and ethanol production

The objective of this work was to evaluate the impacts of spittlebug (Mahanarva fimbriolata) attack on sugarcane quality and ethanol production. Technological and microbiological parameters of juice and fermentation process were evaluated in ten fermentation cycles and two harvest seasons. Treatments consisted of different spittlebug stalk damage levels: control, with 100% of apparently healthy stalks; medium, with 15% of damaged or dry stalks (DDS); high, with 30% of DDS; and very high, with 60% of DDS. Spittlebug attack caused significant losses in cane quality, reducing total soluble solids, sucrose content, total reducing sugars, and pH, and increasing total phenolic compounds, and total and volatile juice acidity. The fermentation process was also significantly affected, resulting in lower ethanol content in wine. There was an increase in acetaldehyde concentration in the distillate. The spittlebug attack caused negative impacts on sugarcane quality and fermentation process, and these impacts are stronger in late season harvests.

Changes in the isoflavone profile and in the chemical composition of tempeh during processing and refrigeration

The objective of this work was to analyze changes in the isoflavone profile, determined by high performance liquid chromatography, at different processing stages and after refrigeration of tempeh. For tempeh production, clean soybean grains from cultivars BR 36 (low isoflavone content) and IAS 5 (high) were dehulled, and the separated cotyledons were hydrated and then cooked in boiling water for 30 min. Spores of the fungus Rhizopus microsporus var. oligosporus were inoculated in the cooked and cooled cotyledons, and incubated at 32ºC for 6, 12, 18, and 24 hours in perforated polypropylene bags, for fermentation. The resulting tempeh was stored at 4ºC for 6, 12, 18, and 24 hours. After 24-hour fermentation, isoflavone glucosides were 50% reduced, and the aglycone forms in the tempeh from both cultivars was increased. The malonyl forms reduced 83% after cooking. Less than 24 hours of refrigeration did not affect the isoflavone profile of tempeh from either cultivar, which is a good indicator of its quality. The tempeh maintains the high and low isoflavone content of the cultivars, which indicates that cultivar differences in this trait should be considered when processing tempeh.

Forage potential and silage quality of four varieties of saccharine sorghum

Abstract: The objective of this work was to evaluate four cultivars of saccharine sorghum (Sorghum bicolor) regarding productivity, chemical composition of plant parts, and quality of the ensiling process. The tested varieties of saccharine sorghum were BRS 506, BRS 508, BRS 509, and BRS 511. The experiment was divided into two trials, which assessed: production, morphological composition, and nutritional quality of the saccharine varieties; and fermentation quality and nutritional value of the silage produced from the saccharine varieties. Of the tested varieties, BRS 509 and BRS 511 showed the highest total dry matter (DM) production. The BRS 508 variety presented the highest in vitro digestibility of the whole plant (70.65% DM). During ensiling, the BRS 509 variety showed the lowest DM loss (8.87%). The highest effluent production was observed for BRS 506 and BRS 508, with yields of 521.87 and 393.16 kg Mg-1 ensiled DM, respectively. The BRS 511 variety is the most recommended because of the best results for plant production and nutritional quality. Regarding the ensiling process, BRS 509 presents the lowest fermentation losses and the highest nutritional value of silage.

Estudo da fermentação alcoólica de soluções diluídas de diferentes açucares utilizando microcalorimetria de fluxo

The present study shows that with liquid nitrogen stored inocula of Saccharomyces cerevisiae, and standardized experimental procedure, flow microcalorimetry can be a valuable tool for monitoring in real time the alcoholic fermentation processes on line. The avaliation of cultural conditions contained different carbon sources for alcohol fermentation (sucrose, glucose, fructose, manose, maltose, galactose, molasses, honey and sugar cane) and their effects on the heat output recording is discussed. Some examples of diauxic growth is given, where the microcalorimeters serves to detect the temporal order of succession of alternating metabolic pathways.