Resistance to Starvation of Triatoma rubrofasciata (De Geer, 1773) under Laboratory Conditions (Hemiptera: Reduviidae: Triatominae)

The present work aims at learning the period of resistance to starvation (molting/death) of Triatoma rubrofasciata in different stages of development and the respective loss of weight until death. Eggs of specimens from the greater area of the city of São Luis in the State of Maranhão, Brazil, yielded approximately 300 nymphs. These nymphs were placed in labelled Borrel glasses, in which they were weekly fed on rats (Rattus norvegicus), until reaching the stage to be observed. The experiments were conducted in a climatic chamber regulated at 29 ± 1° C, 70% relative humidity and 12 hr photoperiod. The resistance to starvation increased according to the stage of development, except for adult bugs, whose results were similar to the 3rd stage nymphs. In all these development stages there was an abrupt loss of weight in the first week, followed by a gradual loss until death. Comparing this work with those of other authors, it was observed that T. rubrofasciata is among the less resistant triatomine species.

Chromatin regulation in schistosomes and histone modifying enzymes as drug targets

Only one drug is currently available for the treatment and control of schistosomiasis and the increasing risk of selecting strains of schistosome that are resistant to praziquantel means that the development of new drugs is urgent. With this objective we have chosen to target the enzymes modifying histones and in particular the histone acetyltransferases and histone deacetylases (HDAC). Inhibitors of HDACs (HDACi) are under intense study as potential anti-cancer drugs and act via the induction of cell cycle arrest and/or apoptosis. Schistosomes like other parasites can be considered as similar to tumours in that they maintain an intense metabolic activity and rate of cell division that is outside the control of the host. We have shown that HDACi can induce apoptosis and death of schistosomes maintained in culture and have set up a consortium (Schistosome Epigenetics: Targets, Regulation, New Drugs) funded by the European Commission with the aim of developing inhibitors specific for schistosome histone modifying enzymes as novel lead compounds for drug development.

The potential of different fruit species as food for Harmonia axyridis (Pallas, 1773) (Coleoptera: Coccinellidae)

Harmonia axyridis was detected for the first time in Brazil in 2002. Since then, it has been recorded from the South to the Midwest of the country. Until now, almost all the registered cases were associated with the presence of aphids, its preferred source of food. Due to the occurrence of this species in areas of fruit growing in the South and Southeast of Brazil, the aim of this study was to analyze the preference and use of three different cultivars of fruit. The tests were set at 25ºC ± 1ºC, RH 70% ± 10%, and in a photophase of 12 h and with apples (Gala and Fuji), grapes (Niágara and Rubi), and pears (Williams and Asian). In the undamaged fruit experiment, the insects did not cause any noticeable damage. In the damaged and undamaged fruit experiment, a higher and statistically significant percentage of H. axyridis adults were found in the three damaged fruits. In the different cultivar experiment the Niágara grape, the Gala apple, and the Williams pear were significantly preferred by H. axyridis adults. These results may help in the management of this insect, preventing damage, which have been observed in other places where H. axyridis was introduced.

Prêmio Nobel de Química em 1998: Walter Kohn e John A. Pople

A résumé of the evolution of quantum chemistry methodologies is presented. The pioneering contributions of John A. Pople and Water Kohn, the 1998 Nobel Prize Laureates in Chemistry, to the development of quantum chemistry computational methods for studying the properties of molecules and their interaction is discussed.

Development of a scar marker for Pierce's Disease strains of Xylella fastidiosa

The objective of this research was to develop a primer for a polymerase chain reaction specific for Xylella fastidiosa strains that cause Pierce's Disease (PD) in grapes (Vitis vinifera). The DNA amplification of 23 different strains of X. fastidiosa, using a set of primers REP1-R (5'-IIIICGICGIATCCIGGC-3') and REP 2 (5'-ICGICTTATCIGGCCTAC-3') using the following program: 94 ºC/2 min; 35 X (94 ºC/1 min, 45 ºC/1 min and 72 ºC/1 min and 30 s) 72 ºC/5 min, produced a fragment of 630 bp that differentiated the strains that cause disease in grapes from the other strains. However, REP banding patterns could not be considered reliable for detection because the REP1-R and REP 2 primers correspond to repetitive sequences, which are found throughout the bacterial genome. The amplified product of 630 bp was eluted from the agarose gel, purified and sequenced. The nucleotide sequence information was used to identify and synthesize an specific oligonucleotide for X. fastidiosa strains that cause Pierce's Disease denominated Xf-1 (5'-CGGGGGTGTAGGAGGGGTTGT-3') which was used jointly with the REP-2 primer at the following conditions: 94 ºC/2 min; 35 X (94 ºC/1 min, 62 ºC/1 min; 72 ºC/1 min and 30 s) 72 ºC/10 min. The DNAs isolated from strains of X. fastidiosa from other hosts [almond (Prumus amygdalus), citrus (Citrus spp.), coffee (Coffea arabica), elm (Ulmus americana), mulberry (Morus rubra), oak (Quercus rubra), periwinkle wilt (Catharantus roseus), plums (Prunus salicina) and ragweed (Ambrosia artemisiifolia)] and also from other Gram negative and positive bacteria were submitted to amplification with a pair of primers Xf-1/REP 2 to verify its specificity. A fragment, about 350 bp, was amplified only when the DNA from strains of X. fastidiosa isolated from grapes was employed.

Os elementos republicanos na tolerância de John Locke

John Locke é conhecido, sobretudo, por ser um dos fundadores do liberalismo. No entanto, pesquisas recentes apontam para novas interpretações do pensador inglês. No caminho dessa tendência, o objetivo deste texto é analisar os elementos republicanos na "Carta sobre a tolerância" de Locke.

Fitossociologia de um remanescente de mata mesófila semidecídua urbana, bosque John Kennedy, Araguari, MG, Brasil

RESUMO - (Fitossociologia de um remanescente de mata mesófila semidecídua urbana, Bosque John Kennedy, Araguari, MG, Brasil). Um estudo fitossociológico foi realizado no Bosque Municipal John Kennedy, município de Araguari, MG (48°11'19''W e 18°38'35''S). O bosque, com aproximadamente 11,2 ha, situa-se no perímetro urbano e é formado principalmente por uma mata mesófila semidecídua. No levantamento fitossociológico realizado, amostraram-se, em 1,2 ha, 1827 indivíduos, pertencentes a 46 famílias; 88 gêneros e 113 espécies, com um índice de diversidade de Shannon de H' = 3,75 nats/indivíduo. Os parâmetros fitossociológicos indicam as espécies mais importantes: Licania apetala, Micrandra elata, Copaifera langsdorffii, Astronium nelson-rosae e Alchornea glandulosa, e as famílias mais importantes: Chrysobalanaceae, Euphorbiaceae, Caesalpinaceae, Mimosaceae e Anacardiaceae. A ocorrência de algumas espécies incluidas entre as principais, caracteristicamente, pioneiras (Alchornea glandulosa e Piptocarpha macropoda), ou secundárias (Didymopanax macrocarpum e Maprounea guianensis), pode ser explicada pela existência de algumas áreas de clareiras, no interior dessa mata semidecídua, mas que, aparentemente, ainda não interferiram na estrutura da mata como um todo.

Lei e ordem econômica no pensamento de John R. Commons

The paper has analyzed John R. Commons' contribution to the comprehension of Law and Economics relationship. In contrast to the orthodox economics, Commons has shown that the capitalist economic order emerges and functions regulated by laws and institutions. These approaches made possible to him to understand the nature of the modern capitalist wealth and the problems that time introduces in economic transactions.

Continuidade ou ruptura? Uma análise de alguns aspectos da filosofia social de John Stuart Mill, Alfred Marshall e John Maynard Keynes

O artigo argumenta que é possível se falar em uma 'tradição' no campo de filosofia social e econômica unindo as obras de J.S. Mill e Alfred Marshall e J.M. Keynes. Essa 'tradição' pode ser caracterizada pelas seguintes concepções: (a) pela rejeição moral aos valores aquisitivos do capitalismo; (b) pela visão de que o sistema capitalista seria incapaz de resolver de forma espontânea as questões das desigualdades de renda e riqueza e da pobreza; (c) pela ideia de que, por uma questão de garantia de liberdade e da diversidade, além de por questões de eficiência econômica, dever-se-ia deixar a iniciativa individual agir livremente nas esferas em que é capaz de engendrar bons resultados, mas que o Estado deveria intervir, quando essa falha, atuando em benefício da coletividade; (d) pela crença de que seria possível melhorar significativamente esse sistema por meio de mudanças pontuais e graduais.

Teoria da justiça de John Jawls: entre o liberalismo e o comunitarismo

O objetivo do presente artigo é realizar uma análise da teoria da justiça como eqüidade de John Rawls em A Theory of Justice e no Political Liberalism, destacando seu modelo de complementaridade entre o deontológico e o procedimental com o teleológico e substancial, buscando responder algumas das críticas levantadas por autores comunitaristas à teoria rawlsiana de justiça e procurando apontar para suas semelhanças. Parto das críticas dos comunitaristas à teoria da justiça como eqüidade; posteriormente, analiso os aspectos teleológicos contidos em seu modelo deontológico; em seguida, destaco a intrínseca relação entre o procedimental e o substancial; por fim, apresento as respostas às críticas comunitaristas à Rawls, destacando a especificidade de sua concepção de justiça.