Distribution of versican and hyaluronan in the mouse uterus during decidualization

Preparation for embryo implantation requires extensive adaptation of the uterine microenvironment. This process consists of cell proliferation and cell differentiation resulting in the transformation of endometrial fibroblasts into a new type of cell called decidual cell. In the present study, we followed the space-time distribution of versican and hyaluronan (HA) in different tissues of the uterus before and after embryo implantation. Fragments of mouse uteri obtained on the fourth, fifth, sixth and seventh days of pregnancy were fixed in Methacarn, embedded in Paraplast and cut into 5-µm thick sections. HA was detected using a biotinylated fragment of the proteoglycan aggrecan, which binds to this glycosaminoglycan with high affinity and specificity. Versican was detected by a polyclonal antibody. Both reactions were developed by peroxidase methods. Before embryo implantation, both HA and versican were present in the endometrial stroma. However, after embryo implantation, HA disappeared from the decidual region immediately surrounding the implantation chamber, whereas versican accumulated in the same region. The differences observed in the expression of HA and versican suggest that both molecules may participate in the process of endometrial decidualization and/or embryo implantation.

Abundancia y distribución de las especies ícticas (Osteichthyes) del río San Francisco-Cosquín, Córdoba, Argentina

It was made the characterization of the San Francisco-Cosquín Mountain River fish community, Córdoba, Argentina. Samples of fish were taken from selected sites from November 1998 to November 1999. Eleven species were recorded, eight of which are first reports for this river. Relative abundance for each species, species richness, diversity and dominance were estimated. Bryconamericus iheringi (Boulenger, 1887) y Jenynsia multidentata (Jenyns, 1842) were the most abundant.

Population dynamics of intermediate snail hosts of Fasciola hepatica and some environmental factors in San Juan y Martinez municipality, Cuba

The variation of abundances of intermediate snail hosts of Fasciola hepatica in Cuba (Fossaria cubensis and Pseudosuccinea columella) was studied during one year under natural conditions at five sampling sites in San Juan y Martinez municipality, Pinar del Rio province, Cuba. The effect of some environmental variables on the lymnaeid abundances was also studied. A canonical correspondence analysis showed that both species do not generally occur together in the same habitat and that most factors affect them in an opposite fashion, although both of them correlate positively through time to the diversity of the habitats. F. cubensis prefers the sites that are in or closer to the city whereas P. columella is more abundant in rural sites. Lymnaeid abundances are mainly affected by nitrite and nitrate concentrations as well as by the abundance of the thiarid Tarebia granifera. F. cubensis is more abundant in polluted habitats with low densities (or absence) of T. granifera whereas P. columella prefers cleaner habitats and can coexist with the thiarid, even at its higher densities. The implications of divergent preferences of the two lymnaeids for the control of fasciolosis are discussed.

Embarazo, parto y puerperio: creencias y prácticas de parteras en San Luis Potosí, México

OBJETIVO: Documentar las creencias y las prácticas de las parteras tradicionales respecto al embarazo parto y puerperio. MÉTODOS: Aprovechando un curso de capacitación de 160 parteras tradicionales locales de las regiones Media y Huasteca del Estado de San Luis Potosí, México, dos de las enfermeras-instructoras entrevistaron a 25 de los capacitandos, apoyadas por un miembro de las comunidades náhuatl y tenek. La identidad de los participantes se mantiene anónima y se obtuvo permiso para publicar los resultados mediante consentimiento informado. RESULTADOS: Las prácticas de las parteras (os) tradicionales son comunes en los grupos sociales que carecen de servicios de salud. Sus funciones no están limitadas al parto e incluyen nutrición, cuidados prenatales, del puerperio y la lactancia, así como apoyo afectivo emocional de las madres parturientas y sus familiares. En este estudio se registraron varios mitos y terapias tradicionales. CONCLUSIÓN: Los recursos de la terapia tradicional y de la atención primaria de la salud de las parteras Náhuatl y Tenek son útiles y benéficos para los cuidados perinatales.

El primer aporte de los obispos de Brasil a la codificación del derecho canónico de 1917: los 'postulata' de los arzobispos de San Salvador de Bahía y de Rio de Janeiro

La redacción del primer Código de Derecho Canónico que tuvo la Iglesia latina fue ordenada por el Papa san Pío X en 1904. La tarea codificadora, empero, no fue obra de un grupo cerrado de expertos, sino que tuvo en cuenta el parecer del episcopado latino, el que fue consultado en dos momentos diferentes y en ambos fueron consultados los obispos de las provincias eclesiásticas de Brasil. En este trabajo se estudia, a partir de la documentación guardada en el Archivo Secreto Vaticano, el aporte de los obispos de las provincias eclesiásticas de Brasil en el primero de dichos momentos.