Imaturos de mosquito (Diptera, Culicidae) de áreas urbana e rural no norte do Estado do Paraná, Brasil

The results of larval collections of mosquitoes from artificial containers and natural breeding at urban and rural areas carried out at Sertaneja, northern State Paraná, Brazil, from February to April, 1995, are presented. Among the 4534 immature forms collected, belonging to 21 species or species-groups, the species with higher density were Aedes aegypti (Linnaeus, 1762), Aedes albopictus (Skuse, 1894), Culex quinquefasciatus Say, 1823 and Limatus durhami Theobald, 1901.

Feeding of two Cichlidae species (Perciformes) in an hypertrophic urban lake

Diet of two cichlid species, Cichlasoma facetum (Jenyns, 1842), and Gymnogeophagus rhabdotus Hensel, 1870, was studied in Rodó Lake, an urban hypertrophic lake in Uruguay. The stomach contents from 192 individuals of C. facetum and 202 of G. rhabdotus, obtained through seasonal sampling in the year 2000, were analyzed. The occurrence frequency and the alimentary importance index of each food item were calculated for each season and size class in both species. Cichlasoma facetum fed upon insects (mainly chironomid larvae and pupae), fish (Cnesterodon decemmaculatus Jenyns, 1842), and vegetals (algae, periphyton and macrophytes debris); large individuals also fed upon the freshwater shrimp Palaemonetes argentinus Nobili, 1901. Gymnogeophagus rhabdotus consumed zooplankton (mainly copepods), vegetals (algae and detritus) and Chironomidae larvae in a lesser extent.

Descriptions and biological notes of immatures of Microctenochira Difficilis (Coleoptera, Chrysomelidae, Hispinae, Cassidini)

Descriptions and illustrations of egg, larva and pupa of Microctenochira difficilis (Boheman, 1855) are presented for the first time. Biological notes and bionomic informations are also included. Eggs, larvae, pupae and adults were collected on Tabebuia sp. (Bignoniaceae) in Monjolinho arboretum at Centro Experimental Santa Elisa, Campinas, State of São Paulo, and kept in laboratory for rearing.

Evaluación de palaemonetes argentinus (Decapoda, Natantia) en el control biológico de larvas de Culex pipiens (Diptera, Culicidae) en condiciones de laboratorio

The predator-prey relationship of Palaemonetes argentinus Nobili, 1901 and Culex pipiens s.l. larvae was studied under laboratory conditions. The prawns were separated in two groups, isolated and grouped ones. Mosquitoe larvae were offered to prawns in two forms, limited and unlimited offer to both groups. In the first analysis, values of predation did not differ significantly between males and females of P. argentinus. Predation in 24 h was 14,9 ± 4,2 larvae/prawn. A reduction of predation was observed with unlimited offer for the same hour, during all the experiment. Predation with unlimited offer was higher on the first day than on the second, but returned to high values on the third day, for both groups. In the grouped experiment, predation with limited offer was total on the first days, decreasing to the last day. As a result, P. argentinus was considered an efficient predator of C. pipiens s.l. larvae under laboratory conditions.

Larvas de simulídeos (Diptera, Simuliidae) do centro oeste, sudeste e sul do Brasil, parasitadas por microsporídeos (Protozoa) e mermitídeos (Nematoda)

A survey of simulid larval parasites was carried out in different localities of the states of Mato Grosso, Minas Gerais, São Paulo, Paraná, Santa Catarina and Rio Grande do Sul, Brazil, from February 1996 to May 1998. Prevalences for the microsporidian Polydispyrenia simulii Lutz & Splendore, 1908 were found in Morungaba and Leme, São Paulo, ranging from around 0.7 to 66.7%, depending mainly on the host simulid species. Microsporidiosis was registered in localities of São Paulo, Paraná, Santa Catarina and Rio Grande do Sul. Parasitism by Isomermis sp. (Nematoda, Mermithidae) was found in Simulium larvae from Serra do Japi, ranging from 0.8 to 45.8%, depending on the simulid species and the larval microhabitat in the stream, whether a cemented ramp in a lake outlet or the natural stream bed. Parasitism by mermithids was also found in ten localities. Mycoses caused by Coelomycidium sp. were for the first time recorded for larvae of Simulium (Chirostilbia) pertinax Kollar, 1832.

Culicídeos associados a entrenós de bambu e bromélias, com ênfase em Aedes (Stegomyia) albopictus (Diptera, Culicidae) na Mata Atlântica, Paraná, Brasil

Aedes (Stegomyia) albopictus (Skuse, 1894) larvae were recorded for the first time in the internodes of bamboo cuts found in the Atlantic Forest in the State of Paraná, Brazil. The bamboo, Bambusa sp., was introduced in the area of Imbucuí-Mirim town, across the municipal district of Paranaguá and towards the interior of the Atlantic Forest. A total of 251 larvae of Aedes albopictus (37.9%), Culex (Microculex) spp. (13.9%), Limatus durhami Theobald, 1901 (39.8%), Toxorhynchites sp. (0.4%) and Wyeomyia spp. (8.0%) were collected from bamboo internodes filled with rain and from the terrestrial Bromeliaceae and epiphytas found near the bamboo bushes. Aedes albopictus was found in bamboo internodes containing 30 to 254 ml of rain, sometimes coexisting with Li. durhami. Borders of forest ecosystems should be characterized as risk areas for arbovirosis transmission, since they sustain the circulation of vertebrates and arthropodal hematophagus, not only in the wild but also in the anthropic areas.

Dieta de espécies de Anostomidae (Teleostei, Characiformes) na área de influência do reservatório de Manso, Mato Grosso, Brasil

The diet of four species of Anostomidae (Leporinus friderici Bloch, 1794, L. striatus Kner, 1858, L. elongatus Valenciennes, 1849 and Leporinus sp.) were investigated in the Manso Reservoir, Mato Grosso State, Brazil. Fish were sampled in three sites: upriver, in the main body of the reservoir, and below the dam. Were analized 276 stomachs. The diet was evaluated using the frequency of ocorrence and volumetric methods. Leporinus friderici showed tendency to herbivory, mainly in the upriver site. In the reservoir and below the dam, it consumed large quantities of fish; Isoptera only in the reservoir. Leporinus striatus and L. elongatus have similar diets, consuming Chironomidae larvae (Diptera), whereas Leporinus sp. was more generalist, feeding similar proportions of vegetable, detritus and insect (Chironomidae and Ephemeroptera). The mouths of the species have different positions: terminal in L. friderici, subterminal in L. elongatus and intermediate in L. striatus and Leporinus sp. The diet overlap values were low, except for L. friderici and Leporinus sp. (0.7) in the upriver, and L. striatus and L. elongatus (0.6) below the dam.

Dispersão larval radial pós-alimentar em Lucilia cuprina (Diptera, Calliphoridae): profundidade, peso e distância de enterramento para pupação

Blowflies utilize discrete and ephemeral sites for breeding and larval nutrition. After the exhaustion of food, the larvae begin dispersing to search for sites to pupate or for additional food source, process referred as postfeeding larval dispersal. Some aspects of this process were investigated in Lucilia cuprina (Wiedemann, 1830), utilizing a circular arena to permit the radial dispersion of larvae from the food source in the center. To determine the localization of each pupa, the arena was split into 72 equal sectors from the center. For each pupa, distance from the center of arena, weight and depth were determined. Statistical tests were performed to verify the relation among weight, depth and distance of burying for pupation. It was verified that the larvae that disperse farthest are those with lowest weights. The majority of individuals reached the depth of burying for pupation between 7 and 18 cm. The study of this process of dispersion can be utilized in the estimation of postmortem interval (PMI) for human corpses in medico-criminal investigations.

Exigências térmicas de Culex quinquefasciatus (Diptera, Culicidae) em Pelotas, Rio Grande do Sul, Brasil

The thermal requeriments of Culex quinquefasciatus (Say, 1823) and the number of generations in the year are determined. The colony to obtain eggs, larvae, pupae and adults was established under laboratory conditions. Every stage was maintained at constant temperature (15, 20, 25 and 30ºC), in cameras, with relative humidity of 80% ± 5 and photophase of 12 hours, to settle down the thermal inferior limit and the thermal constant by the method of the hiperbole. The thermal inferior limit to phase of egg, larvae and pupa were respectively 10.0, 9.1 and 10.2ºC, and 10.2ºC to all the aquatic cycle, with a thermal constant of 207.2 degree-day, with the mean of 15.5 generations per year in Pelotas, State of Rio Grande do Sul.

Descrição de larva de Zenithoptera anceps (Odonata, Libellulidae)

The larva of Zenithoptera anceps Pujol-Luz, 1993 is described and figured for the first time. A comparison among the known larvae of Palpopleurinae is presented. A key to the larvae of the neotropical genera of Palpopleurinae is added.

Hydrophilus (Dibolocelus) palpalis (Coleoptera, Hydrophilidae, Hydrophilinae): descrição dos estágios imaturos

Egg case, larvae and pupa of Hydrophilus (Dibolocelus) palpalis are described and illustrated. The aquaria-terraria method was used to rear immature instars in the laboratory.

Feeding ecology in the small neotropical amphisbaenid Amphisbaena munoai (Amphisbaenidae) in southern Brazil

We analyzed the alimentary tract of 66 specimens of Amphisbaena munoai Klappenbach, 1969 from the Serra do Sudeste, state of Rio Grande do Sul, southern Brazil. Forty specimens (60.6%) had prey items in their gut. The diet consisted mainly of small invertebrate prey, such as termites, insect larvae and ants. The most abundant prey item was termites, found in 62.5% of the non empty stomachs. The high number of individual prey items in the majority of stomachs, the small size of the regular prey items, and the absence of gut content in specimens of A. munoai kept alive for about two days, indicate that this species forages very frequently. The predominance of fossorial prey items and the occasional records of nomadic ants lead us to suggest that A. munoai usually feeds underground, and occasionally forages on the surface.

Diet and microhabitat use by two Hylodinae species (Anura, Cycloramphidae) living in sympatry and syntopy in a Brazilian Atlantic Rainforest area

We analyzed the diet and microhabitat use for two Hylodinae anurans (Cycloramphidae), Hylodes phyllodes Heyer & Cocroft, 1986 and Crossodactylus gaudichaudii Duméril & Bibron, 1841, living in sympatry at an Atlantic Rainforest area of Ilha Grande, in southeastern Brazil. The two species live syntopically at some rocky streams. The two species differed strongly in microhabitat use. Hylodes phyllodes occurred mainly on rocks, whereas C. gaudichaudii was observed mostly on the water. Regarding diet, coleopterans, hymenopterans (ants), and larvae were the most important prey item consumed by both species. Data suggest that microhabitat use appears to be an important parameter differentiating these frogs with respect to general resource utilization.

Hexamermis paranaense new species (Nematoda, Mermithidae): a parasite of Diloboderus abderus (Coleoptera, Scarabaeidae) in Argentina

A new species, Hexamermis paranaense n. sp. (Nematoda, Mermithidae), a parasite of larvae of Diloboderus abderus Sturm, 1826 (Coleoptera, Scarabaeidae) is described and illustrated. This new species is characterized by amphids small, amphidial opening pocket-shaped, the anterior portion of the vagina muscularized and slightly protruding with a descending branch forming a loop before joining the uterus, and three rows of genital papillae: the ventrolateral divided in two rows with eight papillae in the outer row and with six papillae in the inner one; the ventral row with four pairs and one single preanal papillae, and with two pairs, a triplete, one pair, a single and one pair postanal papillae.

Sexual and asexual reproduction in Didemnum rodriguesi (Ascidiacea, Didemnidae)

Sexual and asexual reproduction and associated population dynamics were investigated in the colonial ascidian Didemnum rodriguesi Rocha & Monniot, 1993 (Didemnidae) in southern Brazil. Investment in sexual (production of new individuals) and asexual (colony growth) reproduction was compared between seasons. Permanently marked quadrats were repeatedly photographed to measure changes in colonies. Eggs and larvae were counted monthly in collected colonies. This species alternates seasonally between sexual (summer) and asexual (winter) reproduction. In summer, colonies were smaller, brooded eggs and larvae and recruitment rates were greater, while in winter, colony size was larger and eggs and larvae were absent. There is a relationship between fecundity and colony area. Fragmentation and fusion of colonies were similar in summer and winter, as well as mortality. In conclusion, D. rodriguesi has a lifecycle usual for high latitude ascidians with a limited time length for sexual reproduction and alternate investment in sexual and asexual reproduction along the year.