The sandfly fauna, anthropophily and the seasonal activities of Pintomyia spinicrassa (Diptera: Psychodidae: Phlebotominae) in a focus of cutaneous leishmaniasis in northeastern Colombia

This study was conducted to identify the sandfly fauna and the anthropophilic species in a coffee-growing area of Villanueva, Norte de Santander, Colombia, a focus of American cutaneous leishmaniasis, and to analyse the relationship between the most frequent species and rainfall, relative humidity and temperature, with the aim of contributing to epidemiological surveillance in the area. Sandfly collections were performed fortnightly between February 2006-September 2007 using automatic light traps, Shannon traps, protected human bait and aspiration in resting places. A total of 7,051 sandflies belonging to 12 species were captured. Pintomyia spinicrassa (95.7%) predominated. Pintomyia oresbia and Lutzomyia sp. of Pichinde were found in the state of Norte de Santander for the first time. Pi. spinicrassa, Pintomyia nuneztovari, Micropygomyia venezuelensis, Lutzomyia (Helcocyrtomyia) scorzai and Lu. (Helcocyrtomyia) sp. were captured on the protected human bait. A significant association between Pi. spinicrassa abundance and the total rainfall and the average temperature and humidity 10 days before the collection was observed. The dominance of Pi. spinicrassa, a recognised vector of Leishmania braziliensis, especially during the dry periods, indicates that the risk of parasite transmission may increase.

The phlebotomine fauna (Diptera: Psychodidae) of Guaraí, state of Tocantins, with an emphasis on the putative vectors of American cutaneous leishmaniasis in rural settlement and periurban areas

Phlebotomine sandflies were captured in rural settlement and periurban areas of the municipality of Guaraí in the state of Tocantins (TO), an endemic area of American cutaneous leishmaniasis (ACL). Forty-three phlebotomine species were identified, nine of which have already been recognised as ACL vectors. Eleven species were recorded for the first time in TO. Nyssomyia whitmani was the most abundant species, followed by Evandromyia bourrouli, Nyssomyia antunesi and Psychodopygus complexus. The Shannon-Wiener diversity index and the evenness index were higher in the rural settlement area than in the periurban area. The evaluation of different ecotopes within the rural area showed the highest frequencies of Ev. bourrouli and Ny. antunesi in chicken coops, whereas Ny. whitmani predominated in this ecotope in the periurban area. In the rural settlement area, Ev. bourrouli was the most frequently captured species in automatic light traps and Ps. complexus was the most prevalent in Shannon trap captures. The rural settlement environment exhibited greater phlebotomine biodiversity than the periurban area. Ps. complexus and Psychodopygus ayrozai naturally infected with Leishmania (Viannia) braziliensis were identified. The data identified Ny. whitmani as a potential ACL vector in the periurban area, whereas Ps. complexus was more prevalent in the rural environment associated with settlements.

The sandfly fauna (Diptera: Psychodidae: Phlebotominae) of the Parque Estadual da Serra da Tiririca, Rio de Janeiro, Brazil

Cutaneous leishmaniasis (CL) in the state of Rio de Janeiro is sporadic and can be characterised as a peridomestic transmission that occurs in modified natural environments. The aim of this work was to study the fauna and ecological characteristics of sandflies in an environmentally protected area (the State Park of Serra da Tiririca) within the remnants of the Atlantic Forest in the municipalities of Niterói and Maricá and their possible relationship with leishmaniasis. Captures were performed using light traps during the night once a month for one year in both sylvatic environments and areas surrounding homes near the park. A total of 1,037 sandflies were captured, belonging to nine genera and 12 species: Evandromyia tupynambai (34.1%), Migonemyia migonei (20.6%), Brumptomyia cunhai (13.8%), Micropygomyia schreiberi (9.7%), Psathyromyia lanei (6.5%), Brumptomyia nitzulescui (5.7%), Evandromyia edwardsi (5.4%), Nyssomyia intermedia (2.8%), Evandromyia cortelezzii (0.6%), Pintomyia bianchigalatiae (0.5%), Lutzomyia longipalpis (0.2%) and Sciopemyia microps (0.1%). Both Mg. migonei and Ny. intermedia may be acting as vectors of CL in this area.

Large-scale patterns in morphological diversity and species assemblages in Neotropical Triatominae (Heteroptera: Reduviidae)

We analysed the spatial variation in morphological diversity (MDiv) and species richness (SR) for 91 species of Neotropical Triatominae to determine the ecological relationships between SR and MDiv and to explore the roles that climate, productivity, environmental heterogeneity and the presence of biomes and rivers may play in the structuring of species assemblages. For each 110 km x 110 km-cell on a grid map of America, we determined the number of species (SR) and estimated the mean Gower index (MDiv) based on 12 morphological attributes. We performed bootstrapping analyses of species assemblages to identify whether those assemblages were more similar or dissimilar in their morphology than expected by chance. We applied a multi-model selection procedure and spatial explicit analyses to account for the association of diversity-environment relationships. MDiv and SR both showed a latitudinal gradient, although each peaked at different locations and were thus not strictly spatially congruent. SR decreased with temperature variability and MDiv increased with mean temperature, suggesting a predominant role for ambient energy in determining Triatominae diversity. Species that were more similar than expected by chance co-occurred near the limits of the Triatominae distribution in association with changes in environmental variables. Environmental filtering may underlie the structuring of species assemblages near their distributional limits.

Phlebotomine fauna, natural infection rate and feeding habits of Lutzomyia cruzi in Jaciara, state of Mato Grosso, Brazil

Visceral leishmaniasis (VL) in Brazil is transmitted by the phlebotomine Lutzomyia longipalpis and in some midwestern regions by Lutzomyia cruzi. Studies of the phlebotomine fauna, feeding habits and natural infection rate by Leishmania contribute to increased understanding of the epidemiological chain of leishmaniases and their vectorial capacity. Collections were performed in Jaciara, state of Mato Grosso from 2010-2013, during which time 2,011 phlebotomines (23 species) were captured (68.70% Lu. cruzi and 20.52% Lutzomyia whitmani). Lu. cruzi females were identified by observing the shapes of the cibarium (a portion of the mouthpart) and spermatheca, from which samples were obtained for polymerase chain reaction to determine the rates of natural infection. Engorged phlebotomines were assessed to identify the blood-meal host by ELISA. A moderate correlation was discovered between the number of Lu. cruzi and the temperature and the minimum rate of infection was 6.10%. Twenty-two females were reactive to the antisera of bird (28%), dog (3.30%) and skunk (1.60%). We conclude that Lu. cruzi and Lu. whitmani have adapted to the urban environment in this region and that Lu. cruzi is the most likely vector of VL in Jaciara. Moreover, maintenance of Leishmania in the environment is likely aided by the presence of birds and domestic and synanthropic animals.

New classification of natural breeding habitats for Neotropical anophelines in the Yanomami Indian Reserve, Amazon Region, Brazil and a new larval sampling methodology

Here we present the first in a series of articles about the ecology of immature stages of anophelines in the Brazilian Yanomami area. We propose a new larval habitat classification and a new larval sampling methodology. We also report some preliminary results illustrating the applicability of the methodology based on data collected in the Brazilian Amazon rainforest in a longitudinal study of two remote Yanomami communities, Parafuri and Toototobi. In these areas, we mapped and classified 112 natural breeding habitats located in low-order river systems based on their association with river flood pulses, seasonality and exposure to sun. Our classification rendered seven types of larval habitats: lakes associated with the river, which are subdivided into oxbow lakes and nonoxbow lakes, flooded areas associated with the river, flooded areas not associated with the river, rainfall pools, small forest streams, medium forest streams and rivers. The methodology for larval sampling was based on the accurate quantification of the effective breeding area, taking into account the area of the perimeter and subtypes of microenvironments present per larval habitat type using a laser range finder and a small portable inflatable boat. The new classification and new sampling methodology proposed herein may be useful in vector control programs.

Multiple reproductives in nests of the Neotropical termite Constrictotermes cyphergaster (Isoptera, Termitidae, Nasutitermitinae)

Constrictotermes cyphergaster (Silvester, 1901) builds arboreal nests in Brazilian Cerrado stricto sensu, which are frequently cohabited by inquilines termites and by termitophiles. In a study made at the Parque Estadual da Serra de Caldas Novas, Goiás, Brazil, there were four nests with multiple reproductives, and secondary reproductives were found in 26 nests. Nymphs of Inquilinitermes were more abundant (9,47%) than nymphs of C. cyphergaster (0,78%).

Perfil da fauna de himenópteros parasitóides (Insecta, Hymenoptera) em uma área de Mata Atlântica do Parque Estadual da Fonte Grande, Vitória, ES, Brasil

Survey of the hymenopterous parasitoids (Insecta, Hymenoptera) from the Parque Estadual da Fonte Grande, Vitória, ES, Brazil. Parasitoids Hymenoptera were collected in a trail inside the Parque Estadual da Fonte Grande (20º17'51.7''S; 40º20'13.1''W), located in an area of the Atlantic Forest, during the period of June 2000 to July 2001. A total of 4,595 specimens, belonging to 28 families, have been collected. Braconidae, Eulophidae, and Scelionidae, were the most common families, with relative abundance of 22.5%, 14.32%, and 13.12%, respectively. Sixteen families showed the relative frequency lower than 1%. A preliminary list with 47 genera of some of the families is included.

Análise da riqueza da fauna de Braconidae (Hymenoptera, Ichneumonoidea) em remanescentes naturais da Área de Proteção Ambiental (APA) de Descalvado, SP

Analysis of the Braconidae (Hymenoptera: Ichneumonoidea) fauna richness in natural remnants of the Área de Proteção Ambiental (APA) of Descalvado, SP. A survey of the Braconidae fauna on fragmentary natural environments at northeast São Paulo State was conducted using Malaise traps installed in five sites of Área de Proteção Ambiental of Descalvado. A total of 2,262 specimens, representing 22 subfamilies and 94 genera, was sampled throughout a period of sixteen months (from May, 1999 to August, 2000). Biological data were obtained from current literature and behavior patterns of host utilization for the studied fauna, mainly Braconidae which have been successful in control of agricultural insect pests. Statistical analyses indicated that the asymptote of the genera richness has been approached using this sampling method. This is the most complete survey yet available from natural fragmentary areas of the Descalvado Braconidae fauna. Patterns of distribution and richness of the Braconidae genera were established. Cluster Analysis was adopted taking as attribute the number of Braconidae genera.

Revision of the Neotropical Xanthandrus Verral (Diptera, Syrphidae)

The Neotropical genus Xanthandrus Verral, 1901 is revised. Six species are redescribed: X. bucephalus (Wiedemann, 1830), X. cubanus Fluke, 1936, X. mellinoides (Macquart, 1846), X. mexicanus Curran, 1930, X. nitidulus Fluke, 1937, and X. plaumanni Fluke, 1937. Three species are included based on original descriptions: X. flavomaculatus Shannon, 1927, X. palliatus (Fluke, 1945), and X. simplex (Loew, 1861). New synonyms proposed: Argentinomyia longicornis (Walker, 1837) = Xanthandrus biguttatus Hull, 1945 syn. nov., and Xanthandrus bucephalus (Wiedemann, 1830) = Melanostoma quadrinotata Bigot, 1884 syn. nov. Description of terminalia, a key for Neotropical species, and illustrations are also presented.

Unanthribus, um novo gênero Neotropical de Ptychoderini Jekel, 1855 (Coleoptera, Anthribidae, Anthribinae)

Unanthribus gen. nov. is proposed to include Unanthribus maximus sp. nov. (type species) described from Brazil (Pará), and U. grandis (Jordan, 1911) comb. nov., which is redescribed. The species are illustrated and keyed.

Fauna de Muscidae (Diptera) em três localidades do Estado do Paraná, Brasil, capturada por armadilha Malaise

During two years, from August 1986 to July 1988, the entomofauna of some preserved areas of Parana State, southern Brazil, was sampled in a project called "Levantamento da Fauna Entomológica no Estado do Paraná (PROFAUPAR)". Specimens of Muscidae (Diptera) were sorted out from the material collected using Malaise traps in three of the eight sites sampled (Colombo, Ponta Grossa and Guarapuava) in the first year (August 1986 to July 1987). A total of 7,014 specimens of Muscidae was captured and 91 species were identified. Neodexiopsis flavipalpis Albuquerque was the most abundant species in Ponta Grossa (672 specimens) and in Guarapuava (332 specimens). For Colombo, the most abundant species was Neodexiopsis vulgaris Couri & Albuquerque (172 specimens). The highest richness of species and abundance were observed in Ponta Grossa (77 and 3,559 respectively). The total number of specimens and means values of capture were analyzed. Indices of diversity and evenness were used to discuss richness and dominance of species in each locality. Besides using ecological indices, species richness estimators were also used.

Notes and descriptions of Neotropical Macrotomini (Coleoptera, Cerambycidae, Prioninae)

Mallodon vermiculatus sp. nov. (from Panama) and Nothopleurus santacruzensis sp. nov. (from Bolivia) are described and illustrated. Mallodon dasystomus dasystomus (Say, 1824) = M. mandibularis Lackerbeck, 1998 syn. nov.

Novos táxons em Apomecynini (Coleoptera, Cerambycidae, Lamiinae) da Região Neotropical

Novos táxons descritos em Apomecynini: Eyiaba gen. nov., com duas espécies novas, E. picta sp. nov. (Brasil: São Paulo) e E. itapetinga sp. nov. (Brasil: Bahia); Adetus jacareacanga sp. nov. (Brasil: Pará) e Parmenonta dominicana sp. nov. (República Dominicana). Chave para identificação das espécies de Eyiaba é fornecida.

Comparative morphological study of the Neotropical Cleomenini genera and their transference to the tribes Rhopalophorini Blanchard and Rhinotragini Thomson (Coleoptera, Cerambycidae, Cerambycinae)

In order to investigate the affinities of the hitherto considered Neotropical Cleomenini genera, a detailed morphological comparative study was carried out based on representatives of their type species and other congeneric species. The results, when compared with representatives of Cleomenes Thomson and other non Neotropical Cleomenini genera together with representatives of Rhopalophorini Blanchard and Rhinotragini Thomson, show that: 1) Listroptera Audinet-Serville, 1834, Dihammaphora Chevrolat, 1859, Haenkea Tippmann, 1953, Aguassay Napp & Mermudes, 2001 and Timabiara Napp & Mermudes, 2001 form a very homogeneous group, not related to other Cleomenini, but sharing several synapomorphies with the Rhopalophorini; therefore they are herein transferred to this tribe; 2) the affinities of Dihammaphoroides Zajciw, 1967, were not clearly defined, needing further investigations; nevertheless, the genus is tentatively included in Rhopalophorini due to its morphological similarity with Dihammaphora and allied genera; 3) Pandrosos Bates, 1867 is brought back to Rhinotragini, in which it was originally placed. Therefore, the tribe Cleomenini Lacordaire is no longer represented in the New World.